scholarly journals Isolation, Identification And Prevalence Of Pseudomonas Aeruginosa Isolates From Clinical And Environmental Sources In Onitsha Metropolis, Anambra State

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
C. O. Ezeador ◽  
P. C. Ejikeugwu ◽  
S. N. Ushie ◽  
N. R. Agbakoba
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Prevalence Rate ◽  
Nasal Swab ◽  
Blood Agar ◽  
Biochemical Tests ◽  
Anambra State ◽  
Mueller Hinton ◽  
Bluish Green ◽  
Mueller Hinton Agar

This study was aimed to isolate and identify Pseudomonas aeruginosa and to determine the prevalence rate of isolated P. aeruginosa in Hospitals in Onitsha. Isolates of P. aeruginosa were recovered from both clinical and environmental sources using Cetrimide agar, Blood agar, Mueller-Hinton agar and MacConkey agar.  All the inoculated plates were incubated at 37°C for 24-48 hours and growth was evaluated on these media. Isolates were identified on the basis of standard bacteriological methods like morphology, colonial characteristics, smell in culture, haemolysis, as well as pigment production on these media. All suspected isolates were further characterized and identified by many biochemical reactions. Results revealed that only 22 (18.3%) isolates were P. aeruginosa, while other 98 (81.7%) represented other bacterial genera. The 22 isolates included 19 (86.4%) environmental isolates and 3 (13.6%) clinical isolates. Pseudomonas aeruginosa was most commonly isolated from sink (13.6%), then mops and cleaning buckets (9.1%) and least from theatre bed, nasal swab, floor, disinfectant, ear and wound swab (4.5%). The pigment varied from bluish-green to yellowish-green with a grape-like odor. All isolates were Gram negative, produced β-hemolysis on blood agar and were motile. The biochemical tests showed all the isolates to be strongly positive for catalase, oxidase, citrate, and casein hydrolysis. The prevalence rate of P. aeruginosa is relatively high and its isolation from sources like sinks and theatre bed could be suggestive of the role of this pathogen in nosocomial infections.

scholarly journals Uji Daya Hambat Isolat Actinomycetes sebagai Antibakteri terhadap Pertumbuhan Pseudomonas aeruginosa ATCC 27853 secara In Vitro

2021 ◽  
Vol 10 (1) ◽  
pp. 16
Author(s):  
Saskia Arientika Wahyuningrum ◽  
Meiskha Bahar ◽  
Andri Pramesyanti Pramono
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
P Value ◽  
Gram Positive Bacteria ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
The One ◽  
One Way Anova

Pneumonia is a lung parenchymal infection caused by Pseudomonas aeruginosa.It is Gram negative bacteria that have developed antibiotic resistance. Actinomycetes are Gram-positive bacteria that produce secondary metabolites which have the ability as antimicrobial. Objectives: To identified the ability of Actinomycetes isolates to inhibit the growth of the bacterium Pseudomonas aeruginosa ATCC 27853. The samples in this experiment were from Kebun Raya Bogor that had been rejuvenated on Starch Casein Agar (SCA). Methods: Six dilution series 10-1; 10-2; 10-3; 10-4; 10-5; 10-6 Actinomycetes isolates were used to observe the inhibition zone of P.aeruginosa growth on Mueller Hinton Agar (MHA) media by diffusion method. Results: The effective incubation time occurred at 24 hours, and then it resulted in the average clear zone diameter of 14.70 mm, 10.57 mm, 8.53 mm, 8.47 mm, 6.97 mm, and 5.30 mm. The results of the One – Way Anova test with p-value = 0.000 (p < 0.005) showed some differences at each concentration to inhibit the growth of P.aeruginosa ATCC 27853 at 24 hours incubation period. Conclusion: The most effective concentration of Actinomycetes isolates that can potentially be antibacterial was the concentration of 10-1 with potential solid inhibitory power.Keywords: Actinomycetes, antibacterial, Pseudomonas aeruginosa

scholarly journals Effect of zinc concentration in Mueller-Hinton agar on susceptibility of Pseudomonas aeruginosa to imipenem.

1997 ◽  
Vol 35 (4) ◽  
pp. 1027-1029 ◽  
Author(s):  
J S Daly ◽  
R A Dodge ◽  
R H Glew ◽  
D T Soja ◽  
B A DeLuca ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Zinc Concentration ◽  
Mueller Hinton ◽  
Mueller Hinton Agar

scholarly journals Antimicrobial sensitivity of Avibacterium paragallinarum isolates from layers in the special region of Yogyakarta, Indonesia

2021 ◽  
pp. 1124-1127
Author(s):  
Ima Fauziah ◽  
Widya Asmara ◽  
Agnesia Endang Tri Hastuti Wahyuni
Keyword(s):  
Clinical Symptoms ◽  
Antibiotic Sensitivity ◽  
Biochemical Tests ◽  
Antimicrobial Sensitivity ◽  
Gram Staining ◽  
Mueller Hinton ◽  
Avibacterium Paragallinarum ◽  
Antibiotic Sensitivity Test ◽  
Mueller Hinton Agar ◽  
Special Region

Background and Aim: Infectious coryza (IC) is an upper respiratory disease of chicken caused by Avibacterium paragallinarum. Its clinical symptoms are swollen face and malodorous sinus exudate. This study was conducted to determine the antimicrobial sensitivity of A. paragallinarum isolates from layers in the Special Region of Yogyakarta, Indonesia. Materials and Methods: The samples used in this study were 30 layers that showed IC symptoms. The colony and cell morphology were observed with Gram staining; then, biochemical tests (catalase, oxidase, urease, indole, and motility tests, and carbohydrate fermentation tests using lactose, maltose, mannitol, and sorbitol) were performed to the suspected colony to identify A. paragallinarum. An antibiotic sensitivity test was performed using several antibiotic disks against A. paragallinarum isolates that were cultured on Mueller-Hinton Agar. Results: Out of 30 samples, 24 samples (80%) were found positive for A. paragallinarum. All isolates were sensitive to ampicillin (AMP) and amoxicillin (AML) (100%), and chloramphenicol (C) (91.6%). The antibiotics with intermediate sensitivity were enrofloxacin (79.2%), fosfomycin (75%), and ciprofloxacin (54.2%). The isolates were most resistant to erythromycin (100%), followed by tetracycline (87.5%), streptomycin (83.3%), doxycycline and kanamycin (70.8%), and trimethoprim (62.5%). Conclusion: Out of the total samples, 24 samples (80%) from layers with IC symptoms were identified biochemically as A. paragallinarum. It was sensitive to AMP, AML, and C.

scholarly journals Optochin Revisited: Defining the Optimal Type of Blood Agar for Presumptive Identification of Streptococcus pneumoniae

1998 ◽  
Vol 36 (3) ◽  
pp. 833-834 ◽  
Author(s):  
M. A. Gardam ◽  
M. A. Miller
Keyword(s):  
Streptococcus Pneumoniae ◽  
Susceptibility Testing ◽  
Blood Agar ◽  
Sheep Blood ◽  
Mueller Hinton ◽  
Columbia Agar ◽  
Presumptive Identification ◽  
Mueller Hinton Agar ◽  
Inhibition Zones ◽  
Optimal Type

To determine the optimal media for optochin susceptibility testing of Streptococcus pneumoniae, we measured inhibition zones for 72 S. pneumoniae and 22 Streptococcus viridans isolates on three blood-containing media. Because 15.3, 0, and 22.2% of S. pneumoniae organisms were misidentified on Columbia agar, Trypticase soy agar (TSA), and Mueller-Hinton agar, respectively, each containing sheep blood, we recommend that TSA-sheep blood agar be used.

scholarly journals Cation Components of Mueller-Hinton Agar Affecting Testing of Pseudomonas aeruginosa Susceptibility to Gentamicin

1980 ◽  
Vol 17 (1) ◽  
pp. 55-62 ◽  
Author(s):  
M. A. Kenny ◽  
H. M. Pollock ◽  
B. H. Minshew ◽  
E. Casillas ◽  
F. D. Schoenknecht
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Mueller Hinton ◽  
Mueller Hinton Agar

EVALUATION THE EFFECT OF PRODIGIOSIN ON ALGD EXPRESSION IN CLINICAL ISOLATES OF PSEUDOMONAS AERUGINOSA

2021 ◽  
Vol 74 (9) ◽  
pp. 2265-2276
Author(s):  
Safiya Saad Dhaif ◽  
Nagham Shakir Al-Attar
Keyword(s):  
Gene Expression ◽  
Pseudomonas Aeruginosa ◽  
Biofilm Formation ◽  
Inhibitory Concentration ◽  
Bacterial Isolates ◽  
Quantitative Real Time Pcr ◽  
Biochemical Tests ◽  
Gram Negative ◽  
Vitek 2

The aim of this study is to investigate the role of prodigiosin on P. aeruginosa' s biofilm genes involved in the pathogenicity and persistency of the bacteria; Materials and methods: Gram negative bacterial isolates were taken from burn and wounds specimen obtained from some of Baghdad hospitals. Forty six isolates were identified as Pseudomonas aeruginosa and four isolates as Serratia marcescens by using biochemical tests and VITEK 2 compact system. Susceptibility test was performed for all P. aeruginosa isolates, the results showed that 100% were resistant to Amikacin and 98% were sensitive to Meropenem. Resistant isolates were tested for biofilm formation; the strong and moderate isolates (17) were detected by PCR for AlgD gene presence. From 17 isolates only two had AlgD gene. All serratia isolates were screened for prodigiosin production, which were extracted from the best producer isolate. Minimal inhibitory concentration was assessed for prodigiosin and ciprofloxacin and synergism between them against the two isolates of P. aeruginosa. Results and conclusions: The results showed that the synergistic effect decreased MIC of both prodigiosin and ciprofloxacin by combination, and reduction of biofilm formation was detected. RNA was extracted from the two selected isolates as control in addition to three treatments. The result of quantitative real time PCR showed down regulation in the AlgD gene expression level under some treatments, while there was no gene expression in most treatments with both sub-MICs treatment

Prevalence, diversity and antimicrobial resistance of Salmonella enterica and Pseudomonas aeruginosa isolates from spring water in a rural area of northwestern Morocco

2021 ◽  
Vol 22 (3) ◽  
Author(s):  
Ilham Nassri ◽  
LATIFA TAHRI ◽  
AMAL SAIDI ◽  
NAJIA AMEUR ◽  
MOHAMMED FEKHAOUI
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Rural Area ◽  
Salmonella Enterica ◽  
Spring Water ◽  
Diffusion Method ◽  
Group Iii ◽  
Mueller Hinton ◽  
Mueller Hinton Agar

Abstract. Nassri I, Tahri L, Saidi A, Ameur N, Fekhaoui M. 2021. Prevalence, diversity and antimicrobial resistance of Salmonella enterica and Pseudomonas aeruginosa isolates from spring Water in a rural area of northwestern Morocco. Biodiversitas 22: 1363-1370. The persistence and diversity of serotypes belonging to pathogens in environmental waters including surface and groundwater have been widely documented and that the aquatic environment represents a relatively stable environment for these microorganisms. Study was carried out on the prevalence, diversity, and antibiotic resistance of Salmonella enterica and Pseudomonas aeruginosa isolates taken from 102 samples of spring water in a rural region of Northwestern Morocco (Rabat-Salé-Zemour-Zaer) collected for two seasonal campaigns between March 2010 and June 2011. The search and identification of S. enterica and P. aeruginosa were carried out according to ISO 19250 and ISO 16266 methods respectively. The serotyping of S. enterica and P. aeruginosa was carried out according to the Kauffmann and White and IATS (International Antigenic Typing System) schemes respectively. Antibiotic resistance of S. enterica and P. aeruginosa were carried out by the Mueller-Hinton agar diffusion method (Biorad). S. enterica showed a prevalence of 10.7% with 09 different serotypes circulating including S. paratyphi B, S. brandenburg, S. kentucky, S. group III b (serotype 50: z52: z53,), S. boon, S. tshiongwe, S. assinie, S. togo, and S. tanger. In contrast, P. aeruginosa showed a prevalence of 21.6% with 07 different serotypes circulating including O6, O1, O7, O9, O4, O3, and O10. In this study, antimicrobial resistance revealed that all isolated strains of S. enterica and P. aeruginosa still exhibit a wild resistance phenotype. Contaminated water sources are reservoirs of these pathogens but do not yet present the risk factors for these bacteria to develop antibiotic resistance.

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