Quinolone accumulation in Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus.

ABSTRACT Akway (Drimys piperita) is a woody, evergreen and aromatic plant that belongs to family winteraceae. This plant is used by Sougb tribe lived in Sururey village, District of Anggi, to enhance the vitality of body. The objectives of the research were to determine antimicrobial stability of akway bark extracts influenced by heating time of 100OC, levels of acidity (pH) and salt contents.. Antimicrobial assays were done by using agar well diffusion method against four species of bacteria, i.e. Escherichia coli ATCC25922, Bacillus cereus ATCC10876, Pseudomonas aeruginosa ATCC27853 and Staphylococcus aureus ATCC25923. The results showed that ethanolic extracts of akway bark only inhibited growth of B. cereus and S. aureus with minimum inhibitory concentration 0,99% and 0,89% . The levels of concentration and acidity of ethanol extracts influenced the antimicrobial capacity of extracts.. Whereas heating time on 100OC during 25 minutes and salt contents up to 5% of extract solution did not influence the antimicrobial stability of akway bark extracts. Key words : akway, extracts, antimicrobe, pH, Heating, salt ABSTRAK Akway (Drimys piperita) adalah tumbuhan berkayu, aromatik dan hijau sepanjang tahun dan tergolong dalam suku winteraceae. Tumbuhan ini digunakan oleh Suku Sougb yang bermukim di desa Sururey Distrik Anggi, untuk mengobati malaria dan meningkatkan vitalitas tubuh. Tujuan dari penelitian ini adalah untuk menentukan stabilitas antimikroba ekstrak kulit kayu akway pada waktu pemanasan ekstrak pada 100OC, tingkat keasaman (pH) dan kandungan garam. Pengujian antimikroba dilakukan dengan menggunakan metode difusi agar terhadap empat spesies bakteri yaitu Escherichia coli ATCC25922, Bacillus cereus ATCC10876, Pseudomonas aeruginosa ATCC27853 and Staphylococcus aureus ATCC25923. Hasil menunjukkan bahwa ekstrak etanol kulit kayu akway hanya dapat menghambat bakteri Bacillus cereus dan Staphylococcus aureus dengan konsentrasi hambat minimum masing-masing adalah 0,99% dan 0,89%. Tingkat konsentrasi dan keasaman (pH) mempengaruhi kapasitas antimikroba ekstrak etanol kulit kayu akway. Sedangkan perlakuan pemanasan pada suhu 100OC dengan lama pemanasan sampai dengan 25 menit dan penambahan garam NaCl sampai konsentrasi 5% tidak berpengaruh pada stabilitas antimikroba ekstrak etanol kulit kayu akway.

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Aerobic and Anaerobic Bacteriology of Perirectal Abscess in Children

PEDIATRICS ◽

10.1542/peds.66.2.282 ◽

1980 ◽

Vol 66 (2) ◽

pp. 282-284

Author(s):

Itzhak Brook ◽

William J. Martin

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Chronic Disease ◽

Anaerobic Bacteria ◽

Oral Antibiotic ◽

Incision And Drainage ◽

Group A ◽

Aerobic And Anaerobic ◽

And Staphylococcus Aureus

Aspirates of pus from perirectal abscesses in 28 children were studied for aerobic and anaerobic bacteria. A total of 87 isolates (64 anaerobic and 23 aerobic) were recovered from the patients, an average of 2.3 anaerobes and 0.8 aerobes per specimen. Anaerobic organisms alone were recovered from 15 specimens (54%), and in nine specimens (32%) they were mixed with aerobic organisms. Aerobic organisms were recovered in pure culture in only four patients (14%). The predominant anaerobic organisms were Bacteroides sp (32 isolates, including 14 B fragilis group and seven B melaninogenicus group), Gram-positive anaerobic cocci(15), Fusobacterium sp (six), and Clostridium sp (three). The predominant aerobic organisms were Escherichia coli and Staphylococcus aureus (six of each), group A β-hemolytic streptococci, Pseudomonas aeruginosa, and Proteus morganii (two of each). Five children had a serious underlying chronic disease; three of these had an accompanying bacteremia and two died. Incision and drainage were performed on all patients: some children also received parenteral, or oral antibiotic treatment, or both.

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Diagnóstico molecular de Leptospira spp em matrizes suínas descartadas

Brazilian Journal of Veterinary Research and Animal Science ◽

10.11606/issn.1678-4456.bjvras.2007.26655 ◽

2007 ◽

Vol 44 (1) ◽

pp. 18 ◽

Cited By ~ 3

Author(s):

Sérgio José de Oliveira ◽

Fabrício Bortolanza ◽

Daniel Thompsen Passos ◽

José Antonio Simões Pires-Neto ◽

Luiz Cesar Bello Fallavena ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Proteus Mirabilis ◽

Rio Grande ◽

Leptospira Interrogans ◽

Rio Grande Do Sul ◽

Leptospira Spp ◽

And Staphylococcus Aureus

O Diagnóstico de leptospirose foi efetuado através de método molecular, histopatológico e sorológico em 30 matrizes suínas, descartadas, no Rio Grande do Sul, Brasil. Os objetivos foram comparar a eficiência dos 3 métodos, verificar a sensibilidade de um método de PCR que utiliza um primer único baseado na seqüência de um elemento repetitivo do genoma de Leptospira interrogans, bem como verificar a possível detecção de leptospiras em vários tecidos, incluindo o trato genital. Os animais foram selecionados com base no teste de aglutinação microscópica para incluir tanto animais negativos como positivos e com baixos e altos títulos sorológicos. As maiores freqüências (90 % dos aniamis positivos) e títulos (100 to 800) foram observados para L. interrogans serovar bratislava. Leptospiras foram detectadas por histopatologia em apenas 9 matrizes, todas com altos títulos (pelo menos 100). Um produto de PCR de 438 bp foi observado em todos os animais (fragmentos de 25 rins, 24 úteros e 9 ovidutos). Produtos de PCR similares foram obtidos em DNA de culturas de leptospiras patogênicas, enquanto a não patogênica, L. patoc apresentou um padrão distinto. Nenhum produto de amplificação de DNA de Leptospira spp foi detectado em DNA de culturas de Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella sp, Streptococcus sp and Staphylococcus aureus, ou de sangue de dois leitões. O método molecular foi, assim, específico e o mais eficiente para detectar baixos níveis de patógeno, sendo capaz de diferenciar leptospiras patogênicas e não patogênicas.

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Compatibility of Cephalosporins and Aminogl Ycosides in Peritoneal Dialysis Fluid

Peritoneal Dialysis International ◽

10.1177/089686088300300305 ◽

1983 ◽

Vol 3 (3) ◽

pp. 128-129 ◽

Cited By ~ 7

Author(s):

Carol Loeppky ◽

Eugene Tarka ◽

E. Dale Everett

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Peritoneal Dialysis ◽

In Vitro Studies ◽

Dialysis Fluid ◽

Peritoneal Dialysis Fluid ◽

The Individual ◽

And Staphylococcus Aureus

Often dialysis -associated peritonitis is treated before the results of cultures are known with a cephalosporin and an aminoglycoside in combination. Because there may be antagonism between the individual drugs in such combinations, we have investigated this possibility through the use of timed, killing curves in dialysate effluent. We tested various cephalosporins and aminoglycosides alone and in combination at concentrations usually instilled into the peritoneum and determined their activity against one strain each of Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. The results of these in-vitro studies show no evidence of antagonism but rather suggest an additive effect as evidenced by more rapid killing.

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The production of antibacterial tubing, sutures, and bandages by in situ precipitation of metallic salts

Canadian Journal of Microbiology ◽

10.1139/m91-073 ◽

1991 ◽

Vol 37 (6) ◽

pp. 445-449 ◽

Cited By ~ 5

Author(s):

Samuel R. Farrah ◽

Gregory W. Erdos

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Silver Ions ◽

Silicone Tubing ◽

Metallic Salts ◽

Second Procedure ◽

And Staphylococcus Aureus

Two procedures were used to modify gauze bandages, polyester sutures, silicone tubing, and polyvinyl chloride tubing. In one procedure, the materials were first modified by in situ precipitation of metallic hydroxides and then used to adsorb silver ions. In the second procedure, the materials were soaked in sodium pyrophosphate or sodium chloride, dried, and then soaked in silver nitrate. These procedures produced materials with silver deposited on the surface of the tubing and sutures and both on the surface and within the gauze fibers. The modified materials inhibited the growth of Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus in vitro. Key words: tubing, sutures, bandages, antibacterial.

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