Antibacterial activity of BMS-180680, a new catechol-containing monobactam.

The in vitro activities of a new catechol-containing monobactam, BMS-180680 (SQ 84,100), were compared to those of aztreonam, ceftazidime, imipenem, piperacillin-tazobactam, ciprofloxacin, amikacin, and trimethoprim-sulfamethoxazole. BMS-180680 was often the most active compound against many species of the family Enterobacteriaceae, with MICs at which 90% of the isolates were inhibited (MIC90s) of < or = 0.5 microg/ml for Escherichia coli, Klebsiella spp., Citrobacter diversus, Enterobacter aerogenes, Serratia marcescens, Proteus spp., and Providencia spp. BMS-180680 had moderate activities (MIC90s of 2 to 8 microg/ml) against Citrobacter freundii, Morganella morganii, Shigella spp., and non-E. aerogenes Enterobacter spp. BMS-180680 was the only antibiotic evaluated that was active against >90% of the Pseudomonas aeruginosa (MIC90, 0.25 microg/ml), Burkholderia cepacia, and Stenotrophomonas maltophilia (MIC90s, 1 microg/ml) strains tested. BMS-180680 was inactive against most strains of Pseudomonas fluorescens, Pseudomonas stutzeri, Pseudomonas diminuta, and Burkholderia pickettii. BMS-180680 was moderately active (MIC90s of 4 to 8 microg/ml) against Alcaligenes spp. and Acinetobacter lwoffii and less active (MIC90, 16 microg/ml) against Acinetobacter calcoaceticus-Acinetobacter baumanii complex. BMS-180680 lacked activity against gram-positive bacteria and anaerobic bacteria. Both tonB and cir fiu double mutants of E. coli had greatly decreased susceptibility to BMS-180680. Of the TEM, PSE, and chromosomal-encoded beta-lactamases tested, only the K1 enzyme hydrolyzed BMS-180680 to any measurable extent. Like aztreonam, BMS-180680 bound preferentially to penicillin-binding protein 3. The MICs of BMS-180680 were not influenced by the presence of hematin or 5% sheep blood in the test medium or with incubation in an atmosphere containing 5% CO2. BMS-180680 MICs obtained under strict anaerobic conditions were significantly higher than those obtained in ambient air.

Download Full-text

Antibacterial Spectrum of a Novel Des-Fluoro(6) Quinolone, BMS-284756

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.44.12.3351-3356.2000 ◽

2000 ◽

Vol 44 (12) ◽

pp. 3351-3356 ◽

Cited By ~ 133

Author(s):

Joan C. Fung-Tomc ◽

Beatrice Minassian ◽

Benjamin Kolek ◽

Elizabeth Huczko ◽

Lauren Aleksunes ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Enterococcus Faecalis ◽

Stenotrophomonas Maltophilia ◽

Anaerobic Bacteria ◽

Pseudomonas Stutzeri ◽

Resistant Strains ◽

Vancomycin Resistant ◽

Antibacterial Spectrum ◽

Broad Antibacterial Spectrum

ABSTRACT The in vitro spectrum of a novel des-fluoro(6) quinolone, BMS-284756, was compared with those of five fluoroquinolones (trovafloxacin, moxifloxacin, levofloxacin, ofloxacin, and ciprofloxacin). BMS-284756 was among the most active and often was the most active quinolone against staphylococci (including methicillin-resistant strains), streptococci, pneumococci (including ciprofloxacin-nonsusceptible and penicillin-resistant strains), andEnterococcus faecalis. BMS-284756 inhibited ≈60 to ≈70% of the Enterococcus faecium (including vancomycin-resistant) strains and 90 to 100% of theEnterobacteriaceae strains and gastroenteric bacillary pathogens at the anticipated MIC susceptible breakpoint (≤4 μg/ml). Against the nonfermenters, BMS-284756 inhibited 90 to 100% ofPseudomonas fluorescens, Pseudomonas stutzeri,Stenotrophomonas maltophilia, Flavobacteriumspp., and Acinetobacter spp. and 72% ofPseudomonas aeruginosa strains at 4 μg/ml. Against anaerobic bacteria, BMS-284756 was among the most active, inhibiting essentially all strains tested. It had very low MICs against the fastidious and atypical microbial species, in particular against mycoplasmas or ureaplasmas, Borrelia burgdorferi, chlamydia, and gonococci. These results indicate that with its broad antibacterial spectrum, BMS-284756 should be evaluated clinically for the treatment of community and nosocomial infections.

Download Full-text

Bacterial Identification And Antibiotics Sensitivity Of Ventilator-Associated Pneumonia (VAP) Patients At RSD Dr. Soebandi Jember

Journal of Health Sciences ◽

10.33086/jhs.v14i02.1891 ◽

2021 ◽

Vol 14 (02) ◽

pp. 103-111

Author(s):

Muhammad Ali Shodikin ◽

Mira Haninda Ramadhanty ◽

I Nyoman Semita

Keyword(s):

Burkholderia Cepacia ◽

Antibiotic Sensitivity ◽

Enterobacter Aerogenes ◽

Study Data ◽

Ventilator Associated Pneumonia ◽

Bacterial Identification ◽

Microsoft Excel ◽

Exclusion Criteria ◽

Acinetobacter Baumanii ◽

Antibiotics Sensitivity

ABSTRACT Ventilator-Associated Pneumonia (VAP) is pneumonia in patients who use mechanical ventilator. The empirical antibiotic therapy is given to VAP patients based on bacterial mapping and its antibiotic sensitivity. The aim of this study is to determine bacterial identification and antibiotic sensitivity of VAP patients at RSD dr. Soebandi Jember. This study is a retrospective study, the data collected from medical record of VAP patients from September to October 2019. All samples that is meet the inclusion and exclusion criteria was recruited in this study. Data analysis was use Microsoft Excel 2010. This study obtained 15 samples. The most frequents bacteria that causes VAP is Acinetobacter baumanii. It is also caused by Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterobacter aerogenes, Burkholderia cepacia, Pseudomonas fluorescence, Salmonella arizonae, and Escherichia coli. The bacteria are most sensitive to amikacin, meropenem, and piperacillin-tazobactam and most resistant to cefixime, cefotaxime, and ceftriaxone.

Download Full-text

In-vitro susceptibility of anaerobic bacteria to gar-936, a new glycylcycline

Clinical Microbiology and Infection ◽

10.1046/j.1469-0691.2000.00034-6.x ◽

2000 ◽

Vol 6 (3) ◽

pp. 159-163 ◽

Cited By ~ 46

Author(s):

C. Edlund ◽

C. E. Nord

Keyword(s):

Anaerobic Bacteria ◽

In Vitro Susceptibility

Download Full-text

In Vitro Activity of New Quinolones against Anaerobic Bacteria

Drugs ◽

10.2165/00003495-199500492-00052 ◽

1995 ◽

Vol 49 (Supplement 2) ◽

pp. 233-234

Author(s):

Carl Erik Nord ◽

Ann Lindmark ◽

Ingela Persson

Keyword(s):

Anaerobic Bacteria ◽

Vitro Activity ◽

In Vitro Activity ◽

New Quinolones

Download Full-text

Ambient air pollutant exposure and in vitro fertilization treatment outcomes in Zhengzhou, China

Ecotoxicology and Environmental Safety ◽

10.1016/j.ecoenv.2021.112060 ◽

2021 ◽

Vol 214 ◽

pp. 112060

Author(s):

Wei Dai ◽

Hao Shi ◽

Zhiqin Bu ◽

Yiping Yu ◽

Zhimin Sun ◽

...

Keyword(s):

In Vitro Fertilization ◽

Treatment Outcomes ◽

Ambient Air ◽

Air Pollutant ◽

Vitro Fertilization

Download Full-text

Characterization of Gelatin Hydrogels Cross-Linked with Microbial Transglutaminase as Engineered Skeletal Muscle Substrates

Bioengineering ◽

10.3390/bioengineering8010006 ◽

2021 ◽

Vol 8 (1) ◽

pp. 6

Author(s):

Divya Gupta ◽

Jeffrey W. Santoso ◽

Megan L. McCain

Keyword(s):

Skeletal Muscle ◽

Elastic Modulus ◽

Ambient Air ◽

In Vitro Models ◽

Microbial Transglutaminase ◽

Conventional Culture ◽

Muscle Tissues ◽

Physical Features ◽

Phosphate Buffered Saline

Engineered in vitro models of skeletal muscle are essential for efficiently screening drug safety and efficacy. However, conventional culture substrates poorly replicate physical features of native muscle and do not support long-term culture, which limits tissue maturity. Micromolded gelatin hydrogels cross-linked with microbial transglutaminase (gelatin-MTG hydrogels) have previously been shown to induce C21C2 myotube alignment and improve culture longevity. However, several properties of gelatin-MTG hydrogels have not been systematically characterized, such as changes in elastic modulus during incubation in culture-like conditions and their ability to support sarcomere maturation. In this study, various gelatin-MTG hydrogels were fabricated and incubated in ambient or culture-like conditions. Elastic modulus, mass, and transmittance were measured over a one- or two-week period. Compared to hydrogels in phosphate buffered saline (PBS) or ambient air, hydrogels in Dulbecco’s Modified Eagle Medium (DMEM) and 5% CO2 demonstrated the most stable elastic modulus. A subset of gelatin-MTG hydrogels was micromolded and seeded with C2C12 or primary chick myoblasts, which aligned and fused into multinucleated myotubes with relatively mature sarcomeres. These data are important for fabricating gelatin-MTG hydrogels with predictable and stable mechanical properties and highlight their advantages as culture substrates for engineering relatively mature and stable muscle tissues.

Download Full-text

In Vitro Co-Exposure to CeO2 Nanomaterials from Diesel Engine Exhaust and Benzo(a)Pyrene Induces Additive DNA Damage in Sperm and Cumulus Cells but Not in Oocytes

Nanomaterials ◽

10.3390/nano11020478 ◽

2021 ◽

Vol 11 (2) ◽

pp. 478

Author(s):

Martina Cotena ◽

Mélanie Auffan ◽

Virginie Tassistro ◽

Noémie Resseguier ◽

Jérôme Rose ◽

...

Keyword(s):

Dna Damage ◽

Diesel Engine ◽

Fossil Fuels ◽

Cumulus Cells ◽

Ambient Air ◽

Cell Types ◽

Reproductive Organs ◽

Efficient System ◽

Polycyclic Aromatic

Benzo(a)pyrene (BaP) is a recognized reprotoxic compound and the most widely investigated polycyclic aromatic hydrocarbon in ambient air; it is widespread by the incomplete combustion of fossil fuels along with cerium dioxide nanomaterials (CeO2 NMs), which are used in nano-based diesel additives to decrease the emission of toxic compounds and to increase fuel economy. The toxicity of CeO2 NMs on reproductive organs and cells has also been shown. However, the effect of the combined interactions of BaP and CeO2 NMs on reproduction has not been investigated. Herein, human and rat gametes were exposed in vitro to combusted CeO2 NMs or BaP or CeO2 NMs and BaP in combination. CeO2 NMs were burned at 850 °C prior to mimicking their release after combustion in a diesel engine. We demonstrated significantly higher amounts of DNA damage after exposure to combusted CeO2 NMs (1 µg·L−1) or BaP (1.13 µmol·L−1) in all cell types considered compared to unexposed cells. Co-exposure to the CeO2 NMs-BaP mixture induced additive DNA damage in sperm and cumulus cells, whereas no additive effect was observed in rat oocytes. This result could be related to the structural protection of the oocyte by cumulus cells and to the oocyte’s efficient system to repair DNA damage compared to that of cumulus and sperm cells.

Download Full-text

Prevalence and antibiogram of non-fermenting Gram-negative bacilli in blood stream infections: Study in a tertiary care centre, Western Uttar Pradesh, India

Tropical Doctor ◽

10.1177/0049475520979298 ◽

2021 ◽

pp. 004947552097929

Author(s):

Tarana Sarwat ◽

Mariyah Yousuf ◽

Ambreen S Khan ◽

Dalip K Kakru ◽

Renu Dutta

Keyword(s):

Burkholderia Cepacia ◽

Tertiary Care ◽

Uttar Pradesh ◽

Blood Stream ◽

Care Centre ◽

Tertiary Care Centre ◽

Acinetobacter Baumanii ◽

Blood Stream Infections ◽

Antibiotic Susceptibility Pattern ◽

Gram Negative

Non-fermenting Gram-negative bacilli (NFGNB) are emerging as important cause of blood stream infections. We aimed to determine the prevalence and antibiotic susceptibility pattern of NFGNB isolated from blood of patients with sepsis. We found, in 176 patients, the most common to be Pseudomonas aeruginosa (74) and Acinetobacter baumanii complex (39) followed by Stenotrophomonas maltophilia (16), Sphingomonas paucimobilis (6), Burkholderia cepacia (5) and Ochrobactrum anthropic (1). Generally, organisms showed a good sensitivity towards colistin, carbapenems and fluoroquinolones, whereas cephalosporins were ineffective.

Download Full-text