scholarly journals Analysis of the Clonal Relationship of Serotype O26:H11 Enterohemorrhagic Escherichia coli Isolates from Cattle

2009 ◽  
Vol 75 (21) ◽  
pp. 6947-6953 ◽  
Author(s):  
Lutz Geue ◽  
Sabrina Klare ◽  
Christina Schnick ◽  
Birgit Mintel ◽  
Katharina Meyer ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cluster Analysis ◽  
Human Infection ◽  
Enterohemorrhagic Escherichia Coli ◽  
Clonal Relationship ◽  
Space And Time ◽  
Virulence Markers ◽  
Relationship Of ◽  
Cattle Farms

ABSTRACT Twelve cluster groups of Escherichia coli O26 isolates found in three cattle farms were monitored in space and time. Cluster analysis suggests that only some O26:H11 strains had the potential for long-term persistence in hosts and farms. As judged by their virulence markers, bovine enterohemorrhagic O26:H11 isolates may represent a considerable risk for human infection.

scholarly journals Analysis of the Clonal Relationship of Shiga Toxin-Producing Escherichia coli Serogroup O165:H25 Isolated from Cattle

2006 ◽  
Vol 72 (3) ◽  
pp. 2254-2259 ◽  
Author(s):  
Lutz Geue ◽  
Thomas Selhorst ◽  
Christina Schnick ◽  
Birgit Mintel ◽  
Franz J. Conraths
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Human Infection ◽  
Time And Space ◽  
Cattle Farm ◽  
Virulence Marker ◽  
Clonal Group ◽  
E Coli ◽  
Clonal Relationship ◽  
Relationship Of

ABSTRACT Variations in time and space of a clonal group of Escherichia coli O165:H25 on a cattle farm were monitored. The virulence marker pattern (stx genes, eae gene, hly EHEC gene, katP gene, espP gene, efa gene) suggests that E. coli O165:H25 of bovine origin may represent a risk for human infection.

scholarly journals Rapid Microarray-Based Genotyping of Enterohemorrhagic Escherichia coli Serotype O156:H25/H−/Hnt Isolates from Cattle and Clonal Relationship Analysis

2010 ◽  
Vol 76 (16) ◽  
pp. 5510-5519 ◽  
Author(s):  
Lutz Geue ◽  
Susann Schares ◽  
Birgit Mintel ◽  
Franz J. Conraths ◽  
Elke Müller ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Wide Spectrum ◽  
Simultaneous Detection ◽  
Human Infection ◽  
Enterohemorrhagic Escherichia Coli ◽  
Nucleotide Exchange ◽  
Oligonucleotide Arrays ◽  
E Coli ◽  
Relationship Analysis ◽  
Virulence Markers

ABSTRACT Since enterohemorrhagic Escherichia coli (EHEC) isolates of serogroup O156 have been obtained from human diarrhea patients and asymptomatic carriers, we studied cattle as a potential reservoir for these bacteria. E. coli isolates serotyped by agglutination as O156:H25/H−/Hnt strains (n = 32) were isolated from three cattle farms during a period of 21 months and characterized by rapid microarray-based genotyping. The serotyping by agglutination of the O156 isolates was not confirmed in some cases by the results of DNA-based serotyping as only 25 of the 32 isolates were conclusively identified as O156:H25. In the multilocus sequence typing (MLST) analysis, all EHEC O156:H25 isolates were characterized as sequence type 300 (ST300) and ST688, which differ by a single-nucleotide exchange in the purA gene. Oligonucleotide microarrays allow simultaneous detection of a wider range of EHEC-associated and other E. coli virulence markers than other methods. All O156:H25 isolates showed a wide spectrum of virulence factors typical for EHEC. The stx 1 genes combined with the EHEC hlyA (hlyA EHEC) gene, the eae gene of the ζ subtype, as well as numerous other virulence markers were present in all EHEC O156:H25 strains. The behavior of eight different cluster groups, including four that were EHEC O156:H25, was monitored in space and time. Variations in the O156 cluster groups were detected. The results of the cluster analysis suggest that some O156:H25 strains had the genetic potential for a long persistence in the host and on the farm, while other strains did not. As judged by their pattern of virulence markers, E. coli O156:H25 isolates of bovine origin may represent a considerable risk for human infection. Our results showed that the miniaturized E. coli oligonucleotide arrays are an excellent tool for the rapid detection of a large number of virulence markers.

scholarly journals A long-term study on the prevalence of shiga toxin-producing Escherichia coli (STEC) on four German cattle farms

2002 ◽  
Vol 129 (1) ◽  
pp. 173-185 ◽  
Author(s):  
L. GEUE ◽  
M. SEGURA-ALVAREZ ◽  
F. J. CONRATHS ◽  
T. KUCZIUS ◽  
J. BOCKEMÜHL ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Pulsed Field Gel Electrophoresis ◽  
Single Group ◽  
Term Study ◽  
Virulence Markers ◽  
Long Term Study ◽  
Healthy Cattle ◽  
Cattle Farms

The occurrence of Shiga toxin-producing Escherichia coli (STEC) was studied on four cattle farms. STEC were detected in 29–82% of the cattle. STEC with additional EHEC markers were detected on all farms. The occurrence of the complete virulence marker pattern (stx1 and/or stx2, eae, EHEChlyA, katP, espP) was correlated with the presence of known STEC serotypes. STEC O26[ratio ]H11 and O165[ratio ]H25 with the complete pattern of virulence markers were the most prevalent. STEC O157 (H7/H-) STEC O103[ratio ]H2 and STEC O145[ratio ]H- were found sporadically. Five clonal subgroups of the STEC O26[ratio ]H11 isolates were identified by pulsed-field gel electrophoresis. STEC O26[ratio ]H11 were present in three groups of cattle. This serotype was detected in a single group over the entire fattening period. Most STEC O26[ratio ]H11 with the complete pattern of potential virulence markers were found in clinically healthy cattle. These animals may represent a risk factor for farmers and consumers.

scholarly journals Comparison between human and animal isolates of Shiga toxin-producing Escherichia coli O157 from Australia

2002 ◽  
Vol 128 (3) ◽  
pp. 357-362 ◽  
Author(s):  
N. FEGAN ◽  
P. DESMARCHELIER
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Human Infection ◽  
Pulsed Field Gel Electrophoresis ◽  
Toxin Gene ◽  
Escherichia Coli O157 ◽  
Animal Population ◽  
E Coli ◽  
Virulence Markers ◽  
Animal Isolates

There is very little human disease associated with enterohaemorrhagic Escherichia coli O157 in Australia even though these organisms are present in the animal population. A group of Australian isolates of E. coli O157:H7 and O157:H- from human and animal sources were tested for the presence of virulence markers and compared by XbaI DNA macrorestriction analysis using pulsed-field gel electrophoresis (PFGE). Each of 102 isolates tested contained the gene eae which encodes the E. coli attaching and effacing factor and all but one carried the enterohaemolysin gene, ehxA, found on the EHEC plasmid. The most common Shiga toxin gene carried was stx2c, either alone (16%) or in combination with stx1 (74%) or stx2 (3%). PFGE grouped the isolates based on H serotype and some clusters were source specific. Australian E. coli O157:H7 and H- isolates from human, animal and meat sources carry all the virulence markers associated with EHEC disease in humans therefore other factors must be responsible for the low rates of human infection in Australia.

scholarly journals Identification of Enterohemorrhagic Escherichia coli O26:H− Genes Required for Intestinal Colonization in Calves

2005 ◽  
Vol 73 (3) ◽  
pp. 1735-1743 ◽  
Author(s):  
Pauline M. van Diemen ◽  
Francis Dziva ◽  
Mark P. Stevens ◽  
Timothy S. Wallis
Keyword(s):  
Escherichia Coli ◽  
Public Health Problem ◽  
The United States ◽  
Human Infection ◽  
Enterohemorrhagic Escherichia Coli ◽  
Secreted Proteins ◽  
Type I ◽  
Intestinal Colonization ◽  
Important Public Health Problem ◽  
Type Iii

ABSTRACT Enterohemorrhagic Escherichia coli (EHEC) infections in humans are an important public health problem and are commonly acquired via contact with ruminant feces. The serogroups that are predominantly associated with human infection in the United States and Europe are O157 and O26. Serotypes O157:H7 and O26:H− differ in their virulence and tissue tropism in calves and therefore may colonize calves by distinct mechanisms. The mechanisms underlying EHEC intestinal colonization and pathogenesis are poorly understood. Signature-tagged mutagenesis was used to identify 59 genes of EHEC O26:H− that are required for the intestinal colonization of calves. Our results indicate important roles for locus of enterocyte effacement (LEE)-encoded type III secreted proteins in intestinal colonization. In addition, colonization is facilitated by cytotoxins, putative type III secreted proteins unlinked to the LEE, a putative fimbrial operon, and numerous genes involved in central metabolism and transport and genes of unknown function. Our data also imply that the elaboration of type I fimbriae by EHEC O26:H− is disadvantageous for persistence within the bovine intestines. These observations have important implications for the design of vaccines to control these important zoonotic pathogens.

Molecular Screening, Isolation, and Characterization of Enterohemorrhagic Escherichia coli O157:H7 from Retail Shrimp

2010 ◽  
Vol 73 (1) ◽  
pp. 97-103 ◽  
Author(s):  
ALAGARSAMY SURENDRARAJ ◽  
NIRMALA THAMPURAN ◽  
TOMS C. JOSEPH
Keyword(s):  
Escherichia Coli ◽  
Resistance Index ◽  
Enterohemorrhagic Escherichia Coli ◽  
Direct Pcr ◽  
Strict Adherence ◽  
E Coli ◽  
Virulence Markers ◽  
Isolation And Characterization ◽  
Shrimp Sample ◽  
Fish And Shellfish

Foodborne outbreaks attributed to the contamination of foods with enterohemorrhagic Escherichia coli (EHEC) O157:H7 are a growing global concern. Fish and shrimp samples obtained from different retail fish markets in Cochin, India, were screened by direct PCR assays targeting three important virulence markers of EHEC, the intimin protein (eaeA gene), enterohemolysin (hlyA gene), and Shiga toxin (stx gene). One shrimp (Fenneropenaeus indicus) sample was positive for all these virulence markers, and seven typical E. coli O157:H7 isolates were recovered from the marker-positive shrimp sample. This is the first report of recovery of typical E. coli O157:H7 from fish or shellfish in India. All the typical EHEC isolates had a characteristic reaction in eosin methylene blue agar and belonged to IMViC (indole, methyl red, Voges Proskauer, Simmons citrate reactions) biotype I. These isolates also were negative for sorbitol and methylumbelliferyl-β -glucuronide and exhibited β-hemolytic activity. One isolate showed self-agglutination for E. coli O157 antisera and produced a false-positive reaction with CHROMagar O157. These typical EHEC isolates belonged to a restricted biotype group and had a very low multiple antibiotic resistance index. Isolation of E. coli O157:H7 in fish and shellfish indicates that strict adherence to hygienic handling methods and proper cooking or processing is needed before consumption of these products.

scholarly journals Clonal Relationship among Atypical Enteropathogenic Escherichia coli Strains Isolated from Different Animal Species and Humans

2009 ◽  
Vol 75 (23) ◽  
pp. 7399-7408 ◽  
Author(s):  
Rodrigo A. Moura ◽  
Marcelo P. Sircili ◽  
Luciana Leomil ◽  
Maria Helena Matté ◽  
Luiz R. Trabulsi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gel Electrophoresis ◽  
Animal Species ◽  
Pulsed Field Gel Electrophoresis ◽  
Farm Animals ◽  
Atypical Epec ◽  
Clonal Relationship ◽  
Virulence Markers ◽  
Sources Of Infection ◽  
Animal Isolates

ABSTRACT Forty-nine typical and atypical enteropathogenic Escherichia coli (EPEC) strains belonging to different serotypes and isolated from humans, pets (cats and dogs), farm animals (bovines, sheep, and rabbits), and wild animals (monkeys) were investigated for virulence markers and clonal similarity by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). The virulence markers analyzed revealed that atypical EPEC strains isolated from animals have the potential to cause diarrhea in humans. A close clonal relationship between human and animal isolates was found by MLST and PFGE. These results indicate that these animals act as atypical EPEC reservoirs and may represent sources of infection for humans. Since humans also act as a reservoir of atypical EPEC strains, the cycle of mutual infection of atypical EPEC between animals and humans, mainly pets and their owners, cannot be ruled out since the transmission dynamics between the reservoirs are not yet clearly understood.

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