scholarly journals Molecular Epidemiologic Investigation of Campylobacter coli in Swine Production Systems, Using Multilocus Sequence Typing

2006 ◽  
Vol 72 (8) ◽  
pp. 5666-5669 ◽  
Author(s):  
Siddhartha Thakur ◽  
W. E. Morgan Morrow ◽  
Julie A. Funk ◽  
Peter B. Bahnson ◽  
Wondwossen A. Gebreyes
Keyword(s):  
Multilocus Sequence Typing ◽  
Clonal Complex ◽  
Production Systems ◽  
Genotypic Diversity ◽  
Sequence Type ◽  
Campylobacter Coli ◽  
Swine Production ◽  
Epidemiologic Investigation ◽  
Resistant Strains

ABSTRACT Multilocus sequence typing of 151 Campylobacter coli isolates from swine reared in conventional (n = 74) and antimicrobial-free (n = 77) production systems revealed high genotypic diversity. Sequence type (ST) 1413 was predominant and observed among ciprofloxacin-resistant strains. We identified a C. coli ST 828 clonal complex consisting of isolates from both production systems.

scholarly journals Molecular Epidemiologic Investigation of Campylobacter coli in Swine Production Systems, Using Multilocus Sequence Typing

2008 ◽  
Vol 74 (1) ◽  
pp. 342-342
Author(s):  
Siddhartha Thakur ◽  
W. E. Morgan Morrow ◽  
Julie A. Funk ◽  
Peter B. Bahnson ◽  
Wondwossen A. Gebreyes
Keyword(s):  
Multilocus Sequence Typing ◽  
Production Systems ◽  
Campylobacter Coli ◽  
Swine Production ◽  
Epidemiologic Investigation

Variation in Campylobacter Multilocus Sequence Typing Subtypes from Chickens as Detected on Three Plating Media

2016 ◽  
Vol 79 (11) ◽  
pp. 1986-1989 ◽  
Author(s):  
M. E. BERRANG ◽  
S. R. LADELY ◽  
R. J. MEINERSMANN ◽  
J. E. LINE ◽  
B. B OAKLEY ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Multilocus Sequence Typing ◽  
Clonal Complex ◽  
Sequence Type ◽  
Campylobacter Coli ◽  
Processing Plant ◽  
Full Genome Sequencing ◽  
Multiple Sequence ◽  
Commercial Broiler ◽  
Sequence Types

ABSTRACT The objective of this study was to compare subtypes of Campylobacter jejuni and Campylobacter coli detected on three selective Campylobacter plating media to determine whether each medium selected for different subtypes. Fifty ceca and 50 carcasses (representing 50 flocks) were collected from the evisceration line in a commercial broiler processing plant. Campylobacter was cultured and isolated from cecal contents and carcass rinses on Campy-Cefex, Campy Line, and RF Campylobacter jejuni/coli agars. When a positive result was obtained with all three media, one colony of the most prevalent morphology on each medium was selected for further analysis by full genome sequencing and multilocus sequence typing. Sequence types were assigned according to PubMLST. A total of 49 samples were positive for Campylobacter on all three media. Forty samples contained only C. jejuni, three had only C. coli, and both species were detected in six samples. From 71% of samples, Campylobacter isolates of the same sequence type were recovered on all three media. From 81.6% of samples, isolates were all from the same clonal complex. From significantly fewer samples (26%, P < 0.01), one medium recovered an isolate with a sequence type different from the type recovered on the other two media. When multiple sequence types were detected, six times the medium with the odd sequence type was Campy-Cefex, four times it was Campy-Line, and six times it was RF Campylobacter jejuni/coli. From one sample, three sequence types were detected. In most cases, all three plating media allowed detection of the same type of Campylobacter from complex naturally contaminated chicken samples.

scholarly journals Genotypic Diversity of Methicillin-Resistant Staphylococcus aureus Isolates in Korean Hospitals

2005 ◽  
Vol 49 (8) ◽  
pp. 3583-3585 ◽  
Author(s):  
Kwan Soo Ko ◽  
Yeon-Sook Kim ◽  
Jae-Hoon Song ◽  
Joon-Sup Yeom ◽  
Hyuck Lee ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Multilocus Sequence Typing ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Clonal Complex ◽  
Genotypic Diversity ◽  
Predominant Genotype ◽  
Methicillin Resistant ◽  
Mrsa Strains

ABSTRACT Ninety-six methicillin-resistant Staphylococcus aureus (MRSA) isolates from eight Korean hospitals were analyzed by multilocus sequence typing, SCCmec typing, and spa typing. The predominant genotype was ST5-MRSA-II of clonal complex 5, which was found in 36 isolates from six hospitals, but ST239-MRSA-III was also common. Overall, results showed a notable genotypic diversity of MRSA strains circulating in Korean hospitals.

Molecular Characterization of Methicillin-ResistantStaphylococcus aureusSpread by Neonates Transferred From Primary Obstetrics Clinics to a Tertiary Care Hospital in Korea

2006 ◽  
Vol 27 (6) ◽  
pp. 593-597 ◽  
Author(s):  
Kwan Soo Ko ◽  
Sulhee Park ◽  
Kyong Ran Peck ◽  
Eun Jung Shin ◽  
Won Sup Oh ◽  
...  
Keyword(s):  
Primary Care ◽  
Multilocus Sequence Typing ◽  
Clonal Complex ◽  
Tertiary Care Hospital ◽  
Medical Center ◽  
Tertiary Care ◽  
Sequence Type ◽  
Care Hospital ◽  
Mrsa Strains ◽  
Genotypic Characteristics

Objective.To investigate the characteristics and origins of methicillin-resistantStaphylococcus aureus(MRSA) strains isolated from neonatal patients admitted to a tertiary care hospital from local and primary care obstetrics clinics.Design.Molecular typing study.Setting.A 1,278-bed tertiary care hospital (Samsung Medical Center) and 2 primary obstetrics clinics in Seoul, Korea.Patients.The genotypic characteristics of 12 MRSA samples isolated from 11 neonatal patients transferred from 2 primary care obstetrics clinics to a tertiary care hospital were investigated by means of multilocus sequence typing,spa(staphylococcal protein A) typing, andSCCmectyping. Ten MRSA strains isolated from workers and environments in the associated obstetrics clinics were also investigated.Results.Although the antibiograms of isolates from 2 obstetrics clinics differed, no strain showed multidrug resistance to antimicrobials. Multilocus sequence typing analysis showed that all 22 MRSA isolates analyzed in this study had sequence type 1 (with the allelic profile 1-1-1-1-1-1-1), sequence type 493 (62-1-1-1-1-1-1), or a novel sequence type (25-1-1-1-1-1-1) and that all belonged to a single clonal complex (clonal complex 1). Moreover, they all containedSCCmectype IVA and the identicalspatype (UJEBKBP). These genotypic characteristics are similar to those of typical community-associated MRSA strains rather than the hospital-acquired MRSA strains common in Korea.Conclusion.The findings of this study suggest that community-acquired MRSA strains can spread in primary care clinics and be imported into tertiary care settings.

scholarly journals Emergence of the First Levofloxacin-Resistant Strains of Streptococcus agalactiae Isolated in Italy

2015 ◽  
Vol 59 (4) ◽  
pp. 2466-2469 ◽  
Author(s):  
G. Piccinelli ◽  
F. Gargiulo ◽  
S. Corbellini ◽  
G. Ravizzola ◽  
C. Bonfanti ◽  
...  
Keyword(s):  
Clonal Complex ◽  
Group B Streptococcus ◽  
Sequence Type ◽  
Fluoroquinolone Resistance ◽  
Topoisomerase Iv ◽  
Double Mutation ◽  
Unknown Significance ◽  
Resistant Strains ◽  
Group B ◽  
High Level

ABSTRACTOf 901 group B streptococcus strains analyzed, 13 (1.4%) were resistant to levofloxacin (MICs of >32 μg/ml for seven isolates, 2 μg/ml for four isolates, and 1.5 μg/ml for four isolates). Mutations in the quinolone resistance-determining regions (QRDRs) of gyrase and topoisomerase IV were identified. A double mutation involving the Ser-81 change to Leu forgyrAand the Ser-79 change to Phe or to Tyr forparCwas linked to a high level of fluoroquinolone resistance. In addition, two other mutational positions inparCwere observed, resulting in an Asp-83-to-Tyr substitution and an Asp-83-to-Asn substitution. Different mutations were also observed ingyrB, with unknown significance. Most levofloxacin-resistant GBS strains were of serotype Ib and belonged to sequence type 19 (ST19) and clonal complex 19 (CC-19). Most of them exhibited theepsilongene.

Associations between capsular serotype, multilocus sequence type, and macrolide resistance inStreptococcus agalactiaeisolates from Japanese infants with invasive infections

2013 ◽  
Vol 142 (4) ◽  
pp. 812-819 ◽  
Author(s):  
M. MOROZUMI ◽  
T. WAJIMA ◽  
Y. KUWATA ◽  
N. CHIBA ◽  
K. SUNAOSHI ◽  
...  
Keyword(s):  
Clonal Complex ◽  
Vaccine Development ◽  
Late Onset ◽  
Group B Streptococcus ◽  
Sequence Type ◽  
Macrolide Resistance ◽  
Invasive Infections ◽  
Resistant Strains ◽  
Group B ◽  
Sequence Types

SUMMARYStreptococcus agalactiae(group B streptococcus; GBS) isolates (n = 150) from infants with invasive infections between 2006 and 2011 were analysed for capsular serotype, multilocus sequence type, and antibiotic susceptibility. In cases with late-onset disease (n = 115), primary meningitis was predominant (62·6%), but represented only 39·1% in cases with early-onset disease (n = 23). The most common serotype was III (58·7%), followed by Ia (21·3%) and Ib (12·7%). Sequence types (STs) of serotype III strains included ST17 (50·0%), ST19 (26·1%), ST335 (18·2%), ST27 (4·5%), and ST1 (1·1%). Predominant STs of serotypes Ia and Ib were ST23 (81·3%) and ST10 (84·2%), respectively. No penicillin-resistant strains were detected, but 22·0% of strains hadmef(A/E),erm(A), orerm(B) genes, which mediate macrolide resistance. A new ST335, possessing anmef(A/E) gene belonging to clonal complex 19 gradually increased in frequency. Improved prevention of invasive GBS infections in infants requires timely identification, and ultimately vaccine development.

scholarly journals Molecular characteristics of Neisseria meningitidis in Qatar

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Manal Mahmoud Hamed ◽  
Fayaz Ahmad Mir ◽  
Emad Bashier Ibrahim Elmagboul ◽  
Abdullatif Al-Khal ◽  
Muna A. Rahman S. Al. Maslamani ◽  
...  
Keyword(s):  
Neisseria Meningitidis ◽  
Multilocus Sequence Typing ◽  
Clonal Complex ◽  
Tertiary Care ◽  
Sequence Type ◽  
The State ◽  
Empirical Treatment ◽  
Molecular Characteristics ◽  
Molecular Pattern ◽  
Hamad Medical Corporation

AbstractThe aim of the current study is to review the molecular characteristics of Neisseria meningitidis (N. meningitidis) in Hamad Medical Corporation, which is the provider of secondary and tertiary care in the state of Qatar. A total of 39 isolates of N. meningitidis from the period of 2013 to 2018 were revived and identified by Vitek, and susceptibility on the basis of the E test was retrieved from the patient’s files. The revived isolates were subjected to multilocus sequence typing. The most common serogroup (19) of N. meningitidis was W135, of which 12 were isolated from blood and CSF. ST-11 was the most predominant ST clonal complex causing N. meningitidis cases (61.53%). Clonal complex ST-41/44 was the second most observed complex (3, 2 of which were related to serogroup B). The most frequent sequence type was 9596 (8 isolates). Determining the molecular pattern of N. meningitidis in Qatar is helpful for understanding the strains circulating in Qatar, and the study of the resistance trend of such strains may be very helpful for empirical treatment of future patients.

scholarly journals Molecular characterization of methicillin-resistantStaphylococcus aureusresistant to tigecycline and daptomycin isolated in a hospital in Brazil

2013 ◽  
Vol 142 (3) ◽  
pp. 479-483 ◽  
Author(s):  
A. N. G. DABUL ◽  
I. L. B. C. CAMARGO
Keyword(s):  
Molecular Characterization ◽  
Clonal Complex ◽  
Type A ◽  
Infection Control Practice ◽  
Pulsed Field Gel Electrophoresis ◽  
Sequence Type ◽  
Control Practice ◽  
Mrsa Infection ◽  
Resistant Strains

SUMMARYWe report the molecular characterization of methicillin-resistantStaphylococcus aureus(MRSA) with resistance to tigecycline and to daptomycin isolated from intensive-care-unit patients in Brazil over a 6-month period. Thirty-six isolates (25 from infection sites, 11 from nasal sites) recovered from 23 patients who presented with MRSA infection during this period were characterized by pulsed-field gel electrophoresis, multilocus sequence typing, staphylococcal cassette chromosomemec(SCCmec) typing, and antimicrobial susceptibility profiling. Ten isolates from six patients and two isolates from different patients were resistant to tigecycline and daptomycin, respectively. Eight pulsotypes were identified and one, type A, accounted for 21 isolates from 12 patients; type A isolates were SCCmecII as were a further nine isolates of other pulsotypes. All but four of the total isolates were sequence type (ST) 5 or ST105 and classified as clonal complex (CC) 5; the historically prevalent lineage in Brazil, ST239-SCCmecIII, was identified in only three patients. Tigecycline-resistant strains were all ST105-SCCmecII and two patients were nasally colonized by strains of the same pulsotype found in infection sites. Two ST5-SCCmecII were daptomycin resistant after 48 h incubation. The origin and mechanism of these resistant strains remains unknown and further studies are warranted to determine whether such clones are becoming endemic in Brazilian hospitals and to assess their impact on infection control practice.

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