scholarly journals Degradation of Methanethiol by Methylotrophic Methanogenic Archaea in a Lab-Scale Upflow Anaerobic Sludge Blanket Reactor

2006 ◽  
Vol 72 (12) ◽  
pp. 7540-7547 ◽  
Author(s):  
F. A. M. de Bok ◽  
R. C. van Leerdam ◽  
B. P. Lomans ◽  
H. Smidt ◽  
P. N. L. Lens ◽  
...  
Keyword(s):  
Treatment Plant ◽  
Methanogenic Archaea ◽  
Archaeal Community ◽  
Upflow Anaerobic Sludge Blanket ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Anaerobic Sludge ◽  
Microbial Composition ◽  
Volumetric Loading ◽  
Anaerobic Sludge Blanket

ABSTRACT In a lab-scale upflow anaerobic sludge blanket reactor inoculated with granular sludge from a full-scale wastewater treatment plant treating paper mill wastewater, methanethiol (MT) was degraded at 30°C to H2S, CO2, and CH4. At a hydraulic retention time of 9 h, a maximum influent concentration of 6 mM MT was applied, corresponding to a volumetric loading rate of 16.5 mmol liter−1 day−1. The archaeal community within the reactor was characterized by anaerobic culturing and denaturing gradient gel electrophoresis analysis, cloning, and sequencing of 16S rRNA genes and quantitative PCR. Initially, MT-fermenting methanogenic archaea related to members of the genus Methanolobus were enriched in the reactor. Later, they were outcompeted by Methanomethylovorans hollandica, which was detected in aggregates but not inside the granules that originated from the inoculum, the microbial composition of which remained fairly unchanged. Possibly other species within the Methanosarcinacaea also contributed to the fermentation of MT, but they were not enriched by serial dilution in liquid media. The archaeal community within the granules, which was dominated by Methanobacterium beijingense, did not change substantially during the reactor operation. Some of the species related to Methanomethylovorans hollandica were enriched by serial dilutions, but their growth rates were very low. Interestingly, the enrichments could be sustained only in the presence of MT and did not utilize any of the other typical substrates for methylotrophic methanogens, such as methanol, methyl amine, or dimethylsulfide.

scholarly journals Microbial and Physicochemical Characteristics of Compact Anaerobic Ammonium-Oxidizing Granules in an Upflow Anaerobic Sludge Blanket Reactor

2010 ◽  
Vol 76 (8) ◽  
pp. 2652-2656 ◽  
Author(s):  
Bing-Jie Ni ◽  
Bao-Lan Hu ◽  
Fang Fang ◽  
Wen-Ming Xie ◽  
Boran Kartal ◽  
...  
Keyword(s):  
Upflow Anaerobic Sludge Blanket ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Uasb Reactor ◽  
Anaerobic Sludge ◽  
Stable Operation ◽  
Ammonium Oxidation ◽  
N Removal ◽  
Start Up ◽  
Anaerobic Sludge Blanket

ABSTRACT Anaerobic ammonium oxidation (anammox) is a promising new process to treat high-strength nitrogenous wastewater. Due to the low growth rate of anaerobic ammonium-oxidizing bacteria, efficient biomass retention is essential for reactor operation. Therefore, we studied the settling ability and community composition of the anaerobic ammonium-oxidizing granules, which were cultivated in an upflow anaerobic sludge blanket (UASB) reactor seeded with aerobic granules. With this seed, the start-up period was less than 160 days at a NH4 +-N removal efficiency of 94% and a loading rate of 0.064 kg N per kg volatile suspended solids per day. The formed granules were bright red and had a high settling velocity (41 to 79 m h−1). Cells and extracellular polymeric substances were evenly distributed over the anaerobic ammonium-oxidizing granules. The high percentage of anaerobic ammonium-oxidizing bacteria in the granules could be visualized by fluorescent in situ hybridization and electron microscopy. The copy numbers of 16S rRNA genes of anaerobic ammonium-oxidizing bacteria in the granules were determined to be 4.6 � 108 copies ml−1. The results of this study could be used for a better design, shorter start-up time, and more stable operation of anammox systems for the treatment of nitrogen-rich wastewaters.

scholarly journals Community and Proteomic Analysis of Anaerobic Consortia Converting Tetramethylammonium to Methane

Archaea ◽  
2017 ◽  
Vol 2017 ◽  
pp. 1-14
Author(s):  
Wei-Yu Chen ◽  
Lucia Kraková ◽  
Jer-Horng Wu ◽  
Domenico Pangallo ◽  
Lenka Jeszeová ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
High Throughput Sequencing ◽  
Molecular Techniques ◽  
Upflow Anaerobic Sludge Blanket ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Uasb Reactor ◽  
Anaerobic Sludge ◽  
Methane Formation ◽  
Proteomic Approach

Tetramethylammonium-degrading methanogenic consortia from a complete-mixing suspended sludge (CMSS) and an upflow anaerobic sludge blanket (UASB) reactors were studied using multiple PCR-based molecular techniques and shotgun proteomic approach. The prokaryotic 16S rRNA genes of the consortia were analyzed by quantitative PCR, high-throughput sequencing, and DGGE-cloning methods. The results showed that methanogenicarchaeawere highly predominant in both reactors but differed markedly according to community structure. Community and proteomic analysis revealed thatMethanomethylovoransandMethanosarcinawere the major players for the demethylation of methylated substrates and methane formation through the reduction pathway of methyl-S-CoM and possibly, acetyl-CoA synthase/decarbonylase-related pathways. Unlike high dominance of oneMethanomethylovoranspopulation in the CMSS reactor, diverse methylotrophicMethanosarcinaspecies inhabited in syntrophy-like association with hydrogenotrophicMethanobacteriumin the granular sludge of UASB reactor. The overall findings indicated the reactor-dependent community structures of quaternary amines degradation and provided microbial insight for the improved understanding of engineering application.

scholarly journals Quali-quantitative characterization of biogas with the temporal behavior of organic load on wastewater treatment plant with upflow anaerobic sludge blanket reactors through measurement in full‑scale systems

2021 ◽  
Vol 56 (4) ◽  
pp. 621-629
Author(s):  
Orlando Antonio Duarte Hernandez ◽  
Ana Caroline Paula ◽  
Gustavo Rafael Collere Possetti ◽  
Mauricio Pereira Cantão ◽  
Miguel Mansur Aisse
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Full Scale ◽  
Upflow Anaerobic Sludge Blanket ◽  
Organic Load ◽  
Anaerobic Sludge ◽  
Time Behavior ◽  
Flow Meter ◽  
Anaerobic Sludge Blanket

This study aims to present the time behavior of wastewater flow parameters, organic matter, biogas flow, biogas composition, and its relations, measured through online sensors, in a municipal wastewater treatment plant (WWTP) operating full-scale upflow anaerobic sludge blanket (UASB) reactors, installed in the south of Brazil. WWTP has online measurement devices to evaluate some physicochemical variables of the sewage and the biogas. The COD analyzer (UV– Vis probe), ultrasonic flow meter, biogas flow meter, and biogas composition analyzer were the equipment used. The monitoring occurred for two time periods each of 72 h and one time period for 48 h in the year 2018. Data were checked with descriptive statistics, data independence was checked through the autocorrelation Box– Ljung test, normality behavior was checked with several tests (Shapiro– Wilk, Kolmogorov–Smirnov, Lilliefors, Anderson–Darling, D’Agostino K2, and Chen–Shapiro), and Spearman’s correlation coefficient was used to evaluate the correlations among the parameters. The mean sewage flow was 345 ± 120 L.s-1; removed organic load was, in average, 48%; biogas quality values were 82.32% ± 3.62% v/v (CH4), 2.66% ± 1.19% v/v (CO2), and 3453 ± 1268 ppm (H2S); and the production per capita obtained was 4.51 ± 1.65 NL.hab-1.d-1. It was estimated an electric power generation of 3118.6 kWh.d-1, which is equivalent to an installed power of 130 KW. The behavior of removed organic load and biogas flow (Nm3.h-1), produced in the treatment plant, showed variable, periodic, and nonstationary time behavior.  

Effect of sulfate and lactate loading rates on the respiration process and microbial population changes measured by ecological indices

2014 ◽  
Vol 71 (1) ◽  
pp. 97-104
Author(s):  
C. García-Saucedo ◽  
F. J. Fernández ◽  
F. M. Cuervo-López ◽  
J. Gómez
Keyword(s):  
Microbial Population ◽  
Random Amplified Polymorphic Dna ◽  
Upflow Anaerobic Sludge Blanket ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Anaerobic Sludge ◽  
Ecological Indices ◽  
Population Changes ◽  
Loading Rates ◽  
Sulfate Reducing

In a sulfate reducing process, increasing loading rates and sulfide accumulation may induce population changes resulting in decreasing effectiveness of the process. Thus, the relationship between microbial metabolism changes and population dynamics was studied. An upflow anaerobic sludge blanket reactor was operated at different sulfate loading rates (SLR), from 290 to 981 mg SO4 − S/L d at a constant carbon/sulfur ratio of 0.75. When the SLR was increased, the total organic carbon and sulfate consumption efficiencies decreased to nearly 30% and 25%, respectively. The acetate and propionate yields increased with increasing SLR and 385 ± 7 mg sulfide-S/L d was reached. The ecological indices, determined by random amplified polymorphic DNA and denaturing gradient gel electrophoresis techniques, diversity and evenness were found to be constant, and similarity coefficient values remained higher than 76%. The results suggest that the microbial population changes were negligible compared with metabolic changes when SLR was increased. The sulfide accumulation did not modify the microbial diversity. The sequencing of 16S rRNA genes showed strains related to sulfate reducing, fermentation, and methanogenesis processes. The results indicated that the decreasing of effectiveness, under the experimental conditions tested, was dependent more on operational parameters than microbial changes.

Buoyant Filter Bio-Reactor (BFBR)–a novel anaerobic wastewater treatment unit

2008 ◽  
Vol 58 (2) ◽  
pp. 373-377
Author(s):  
Soosan J. Panicker ◽  
M. C. Philipose ◽  
Ajit Haridas
Keyword(s):  
Removal Efficiency ◽  
Treatment Plant ◽  
Cod Removal ◽  
Upflow Anaerobic Sludge Blanket ◽  
Organic Loading Rate ◽  
Anaerobic Sludge ◽  
Treatment Unit ◽  
Biogas Yield ◽  
Cod Removal Efficiency ◽  
Anaerobic Sludge Blanket

The Buoyant Filter Bio-Reactor (BFBR) is a novel and very efficient method for the treatment of complex wastewater. Sewage is a complex wastewater containing insoluble COD contributed by fat and proteins. The fat and proteins present in the domestic sewage cause operational problems and underperformance in the Upflow Anaerobic Sludge Blanket Reactor, used now for treating sewage anaerobically. The biogas yield from the BFBR is 0.36 m3/kg COD reduced and the methane content was about 70–80%. Production of methane by anaerobic digestion of organic waste had the benefit of lower energy costs for treatment and is thus environmentally beneficial to the society by providing a clean fuel from renewable feed stocks. The BFBR achieved a COD removal efficiency of 80–90% for an organic loading rate of 4.5 kg/m3/d at a hydraulic retention time of 3.25 hours. The effluent COD was less than 100 mg/l, thus saving on secondary treatment cost. No pretreatment like sedimentation was required for the influent to the BFBR. The BFBR can produce low turbidity effluent as in the activated sludge process (ASP). The land area required for the BFBR treatment plant is less when compared to ASP plant. Hence the problem of scarcity of land for the treatment plant is reduced. The total expenditure for erecting the unit was less than 50% as that of conventional ASP for the same COD removal efficiency including land cost.

scholarly journals Bacterial community dynamics in tropical soil after sewage sludge amendment

2020 ◽  
Vol 82 (12) ◽  
pp. 2937-2947
Author(s):  
Bruna C. Lopes ◽  
Raíssa S. Figueiredo ◽  
Juliana C. Araújo ◽  
Antonio T. Matos
Keyword(s):  
Sewage Sludge ◽  
Bacterial Community ◽  
Community Dynamics ◽  
Tropical Soils ◽  
Tropical Soil ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Anaerobic Sludge ◽  
Microbial Composition ◽  
Bacterial Community Dynamics

Abstract Although the widespread use of sewage sludge in developing countries is common, little is known about how sludge disposal can affect the microbial composition and diversity of tropical soils. We evaluated the effects of the sewage sludges of two types of anaerobic digestors differing, by the biological treatment they have undergone (uplow anaerobic sludge blanket and activated sludge digester), and two different disposal methods (surface and incorporated) on tropical soils. Samples were taken from topsoil (0–10 cm) and analyzed by amplifying the 16S rRNA genes to study the microbial community, and physicochemical analysis was performed concomitantly. The results indicated that, in general, sewage sludge amendment (SSA) significantly changed the tropical soil bacterial community by the sludge type and by application method. Moreover, the redundancy analysis diagram indicates that changes in soil chemical parameters over time due to SSA resulted in changes in the bacterial community's composition, increasing the population responsible for recycling nutrients in the soil.

Pilot study of UASB process treating PTA manufacturing wastewater

1997 ◽  
Vol 36 (6-7) ◽  
pp. 73-82 ◽  
Author(s):  
Sheng-Shung Cheng ◽  
Chiou-Yuan Ho ◽  
Jer-Horng Wu
Keyword(s):  
Acetic Acid ◽  
Pilot Scale ◽  
Upflow Anaerobic Sludge Blanket ◽  
Uasb Reactor ◽  
Anaerobic Sludge ◽  
High Concentration ◽  
Refractory Component ◽  
Anaerobic Biodegradability ◽  
Volumetric Loading ◽  
Anaerobic Sludge Blanket

A pilot-scale upflow anaerobic sludge blanket (UASB) reactor was employed to treat the wastewater of a purified-terephthalic-acid (PTA) manufacturing factory. The performance of UASB reactor in terms of COD removal was achieved 62% at the volumetric loading rate of 2.93 kg COD / m3/day. One of the major constituents, p-toluic acid in PTA wastewater was the refractory component due to the methyl substituent on the aromatic ring, which restricted the biodegradation performance. Moreover, from our study, it was surprising that high concentration of acetic acid would retard the degradation of aromatics in the sludge bed. A control strategy of acetic acid level in the sludge bed was suggested during the start-up period. Comparison of the anaerobic biodegradability of aromatic substituents in PTA wastewater was concluded as the following sequence: –COOH > –CHO ≥ –CH3 in terms of the derivative functional groups based on the benzoic acid. Observation of bacterial population of the sludge granules showed high diversity of syntrophic structure on the biogranular surface as well as acetoclastic methanogens.

scholarly journals Scum yield and effectiveness of a hydrostatic removal device: case study of Laboreaux STP

2017 ◽  
Vol 12 (1) ◽  
pp. 202-210 ◽  
Author(s):  
A. P. Rosa ◽  
A. V. Santos ◽  
A. D. U. C. Schmidt ◽  
J. M. Borges ◽  
C. A. L. Chernicharo
Keyword(s):  
Sewage Treatment ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Domestic Sewage ◽  
Operating Conditions ◽  
Upflow Anaerobic Sludge Blanket ◽  
Anaerobic Sludge ◽  
Yield Coefficient ◽  
Anaerobic Sludge Blanket

This paper aimed to evaluate the effectiveness of a device to remove scum from the inner part of gas-liquid-solid separators of a full-scale upflow anaerobic sludge blanket reactor treating domestic sewage, as well as determining the scum yield coefficient for different reactors’ operating conditions. The experiments were carried out at the Laboreaux sewage treatment plant (Itabira, Minas Gerais, Brazil) and the effectiveness of the scum removal device was fully demonstrated, with median efficiencies ranging from 65 to 90%. The average scum yield varied from 6.8 to 14.6 mL of scum formed per kg COD applied.

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