scholarly journals Genetic Determinants for Cadmium and Arsenic Resistance among Listeria monocytogenes Serotype 4b Isolates from Sporadic Human Listeriosis Patients

2013 ◽  
Vol 79 (7) ◽  
pp. 2471-2476 ◽  
Author(s):  
Sangmi Lee ◽  
M. Rakic-Martinez ◽  
L. M. Graves ◽  
T. J. Ward ◽  
R. M. Siletzky ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Listeria Monocytogenes ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Genetic Determinants ◽  
Chromosomal Locus ◽  
Cadmium Resistance ◽  
Arsenic Resistance ◽  
Content Type ◽  
Serotype 4B

ABSTRACTInListeria monocytogenesserotype 4b isolates from sporadic listeriosis, heavy metal resistance was primarily encountered in certain clonal groups (ECI, ECII, and ECIa). All arsenic-resistant isolates harbored the arsenic resistance cassette previously identified in pLI100; ECIa harbored additional arsenic resistance genes and a novel cadmium resistance determinant in a conserved chromosomal locus.

scholarly journals The Arsenic Resistance-Associated Listeria Genomic Island LGI2 Exhibits Sequence and Integration Site Diversity and a Propensity for Three Listeria monocytogenes Clones with Enhanced Virulence

2017 ◽  
Vol 83 (21) ◽  
Author(s):  
Sangmi Lee ◽  
Todd J. Ward ◽  
Dereje D. Jima ◽  
Cameron Parsons ◽  
Sophia Kathariou
Keyword(s):  
Listeria Monocytogenes ◽  
Genomic Island ◽  
Integration Site ◽  
Pcr Analysis ◽  
Cadmium Resistance ◽  
Arsenic Resistance ◽  
Content Type ◽  
Dna Integration ◽  
Accessory Genes ◽  
Serotype 4B

ABSTRACT In the foodborne pathogen Listeria monocytogenes, arsenic resistance is encountered primarily in serotype 4b clones considered to have enhanced virulence and is associated with an arsenic resistance gene cluster within a 35-kb chromosomal region, Listeria genomic island 2 (LGI2). LGI2 was first identified in strain Scott A and includes genes putatively involved in arsenic and cadmium resistance, DNA integration, conjugation, and pathogenicity. However, the genomic localization and sequence content of LGI2 remain poorly characterized. Here we investigated 85 arsenic-resistant L. monocytogenes strains, mostly of serotype 4b. All but one of the 70 serotype 4b strains belonged to clonal complex 1 (CC1), CC2, and CC4, three major clones associated with enhanced virulence. PCR analysis suggested that 53 strains (62.4%) harbored an island highly similar to LGI2 of Scott A, frequently (42/53) in the same location as Scott A (LMOf2365_2257 homolog). Random-primed PCR and whole-genome sequencing revealed seven novel insertion sites, mostly internal to chromosomal coding sequences, among strains harboring LGI2 outside the LMOf2365_2257 homolog. Interestingly, many CC1 strains harbored a noticeably diversified LGI2 (LGI2-1) in a unique location (LMOf2365_0902 homolog) and with a novel additional gene. With few exceptions, the tested LGI2 genes were not detected in arsenic-resistant strains of serogroup 1/2, which instead often harbored a Tn554-associated arsenic resistance determinant not encountered in serotype 4b. These findings indicate that in L. monocytogenes, LGI2 has a propensity for certain serotype 4b clones, exhibits content diversity, and is highly promiscuous, suggesting an ability to mobilize various accessory genes into diverse chromosomal loci. IMPORTANCE Listeria monocytogenes is widely distributed in the environment and causes listeriosis, a foodborne disease with high mortality and morbidity. Arsenic and other heavy metals can powerfully shape the populations of human pathogens with pronounced environmental lifestyles such as L. monocytogenes. Arsenic resistance is encountered primarily in certain serotype 4b clones considered to have enhanced virulence and is associated with a large chromosomal island, Listeria genomic island 2 (LGI2). LGI2 also harbors a cadmium resistance cassette and genes putatively involved in DNA integration, conjugation, and pathogenicity. Our findings indicate that LGI2 exhibits pronounced content plasticity and is capable of transferring various accessory genes into diverse chromosomal locations. LGI2 may serve as a paradigm on how exposure to a potent environmental toxicant such as arsenic may have dynamically selected for arsenic-resistant subpopulations in certain clones of L. monocytogenes which also contribute significantly to disease.

scholarly journals Genome Sequences of Listeria monocytogenes Strains with Resistance to Arsenic

2017 ◽  
Vol 5 (19) ◽  
Author(s):  
Vikrant Dutta ◽  
Sangmi Lee ◽  
Todd J. Ward ◽  
Nathane Orwig ◽  
Eric Altermann ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Listeria Monocytogenes ◽  
Draft Genome ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Genome Sequences ◽  
Content Type

ABSTRACT Listeria monocytogenes frequently exhibits resistance to arsenic. We report here the draft genome sequences of eight genetically diverse arsenic-resistant L. monocytogenes strains from human listeriosis and food-associated environments. The availability of these genomes will help elucidate the role of heavy-metal resistance in the ecology of L. monocytogenes.

scholarly journals Draft Genome Sequence of Heavy Metal-Resistant Bacillus cereus NWUAB01

2019 ◽  
Vol 8 (7) ◽  
Author(s):  
Olubukola Oluranti Babalola ◽  
Bukola Rhoda Aremu ◽  
Ayansina Segun Ayangbenro
Keyword(s):  
Heavy Metal ◽  
Bacillus Cereus ◽  
Genome Sequence ◽  
Gold Mining ◽  
Draft Genome ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Draft Genome Sequence ◽  
Mining Site ◽  
Content Type

Bacillus cereus NWUAB01 was isolated from a gold-mining site in Vryburg, South Africa, for its multiple heavy metal resistance properties. Here, we report the draft genome sequence of B. cereus NWUAB01 obtained with Illumina sequencing.

scholarly journals Heavy Metal and Disinfectant Resistance of Listeria monocytogenes from Foods and Food Processing Plants

2012 ◽  
Vol 78 (19) ◽  
pp. 6938-6945 ◽  
Author(s):  
Shakir S. Ratani ◽  
Robin M. Siletzky ◽  
Vikrant Dutta ◽  
Suleyman Yildirim ◽  
Jason A. Osborne ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Listeria Monocytogenes ◽  
Food Processing ◽  
Cadmium Resistance ◽  
Content Type ◽  
Ecological History ◽  
Resistance Determinants ◽  
Processing Plants ◽  
History Of ◽  
Serotype 4B

ABSTRACTThe persistence ofListeria monocytogenesin food processing plants and other ecosystems reflects its ability to adapt to numerous stresses. In this study, we investigated 138 isolates from foods and food processing plants for resistance to the quaternary ammonium disinfectant benzalkonium chloride (BC) and to heavy metals (cadmium and arsenic). We also determined the prevalence of distinct cadmium resistance determinants (cadA1,cadA2, andcadA3) among cadmium-resistant isolates. Most BC-resistant isolates were resistant to cadmium as well. Arsenic resistance was encountered primarily in serotype 4b and was an attribute of most isolates of the serotype 4b epidemic clonal group ECIa. Prevalence of the known cadmium resistance determinants was serotype associated:cadA1was more common in isolates of serotypes 1/2a and 1/2b than 4b, whilecadA2was more common in those of serotype 4b. A subset (15/77 [19%]) of the cadmium-resistant isolates lacked the known cadmium resistance determinants. Most of these isolates were of serotype 4b and were also resistant to arsenic, suggesting novel determinants that may confer resistance to both cadmium and arsenic in these serotype 4b strains. The findings may reflect previously unrecognized components of the ecological history of different serotypes and clonal groups ofL. monocytogenes, including exposures to heavy metals and disinfectants.

scholarly journals Draft Genome Sequence of Arthrobacter oryzae TNBS02, a Bacterium Containing Heavy Metal Resistance Genes, Isolated from Soil of Antarctica

2019 ◽  
Vol 8 (4) ◽  
Author(s):  
Yong-Joon Cho ◽  
Ahnna Cho ◽  
Soon Gyu Hong ◽  
Han-Gu Choi ◽  
Ok-Sun Kim
Keyword(s):  
Heavy Metal ◽  
Genome Sequence ◽  
Resistance Genes ◽  
Draft Genome ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Draft Genome Sequence ◽  
Content Type ◽  
Terra Nova Bay ◽  
Victoria Land

Arthrobacter oryzae TNBS02 was isolated from soil at Terra Nova Bay of Victoria Land, Antarctica. The genome consists of a chromosome with 4,248,670 bp which contains a total of 3,994 genes.

scholarly journals Coselection of Cadmium and Benzalkonium Chloride Resistance in Conjugative Transfers from Nonpathogenic Listeria spp. to Other Listeriae

2012 ◽  
Vol 78 (21) ◽  
pp. 7549-7556 ◽  
Author(s):  
S. Katharios-Lanwermeyer ◽  
M. Rakic-Martinez ◽  
D. Elhanafi ◽  
S. Ratani ◽  
J. M. Tiedje ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Benzalkonium Chloride ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Processing Plant ◽  
Cadmium Resistance ◽  
Transfer Resistance ◽  
Content Type ◽  
Plant Environment ◽  
Disinfectant Resistance

ABSTRACTResistance to the quaternary ammonium disinfectant benzalkonium chloride (BC) may be an important contributor to the ability ofListeriaspp. to persist in the processing plant environment. Although a plasmid-borne disinfectant resistance cassette (bcrABC) has been identified inListeria monocytogenes, horizontal transfer of these genes has not been characterized. NonpathogenicListeriaspp. such asL. innocuaandL. welshimeriare more common thanL. monocytogenesin food processing environments and may contribute to the dissemination of disinfectant resistance genes in listeriae, includingL. monocytogenes. In this study, we investigated conjugative transfer of resistance to BC and to cadmium from nonpathogenicListeriaspp. to other nonpathogenic listeriae, as well as toL. monocytogenes. BC-resistantL. welshimeriandL. innocuaharboringbcrABC, along with the cadmium resistance determinantcadA2, were able to transfer resistance to other nonpathogenic listeriae as well as toL. monocytogenesof diverse serotypes, including strains from the 2011 cantaloupe outbreak. Transfer among nonpathogenicListeriaspp. was noticeably higher at 25°C than at 37°C, whereas acquisition of resistance byL. monocytogeneswas equally efficient at 25 and 37°C. When the nonpathogenic donors were resistant to both BC and cadmium, acquisition of cadmium resistance was an effective surrogate for transfer of resistance to BC, suggesting coselection between these resistance attributes. The results suggest that nonpathogenicListeriaspp. may behave as reservoirs for disinfectant and heavy metal resistance genes for other listeriae, including the pathogenic speciesL. monocytogenes.

scholarly journals Draft Genome Sequence of Megaplasmid-Bearing Staphylococcus sciuri Strain B9-58B, Isolated from Retail Pork

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Leena Neyaz ◽  
Anand B. Karki ◽  
Mohamed K. Fakhr
Keyword(s):  
Heavy Metal ◽  
Stress Response ◽  
Antimicrobial Agent ◽  
Genome Sequence ◽  
Draft Genome ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Draft Genome Sequence ◽  
Content Type

Here, we report the genome sequence of the megaplasmid-bearing Staphylococcus sciuri strain B9-58B, isolated from retail pork. This strain contains a 2,761,440-bp chromosome and a 162,858-bp megaplasmid. The genome contains putative genes involved in virulence, the stress response, and antimicrobial agent and heavy metal resistance.

scholarly journals Full-Length Nucleotide Sequences ofmcr-1-Harboring Plasmids Isolated from Extended-Spectrum-β-Lactamase-Producing Escherichia coli Isolates of Different Origins

2016 ◽  
Vol 60 (9) ◽  
pp. 5589-5591 ◽  
Author(s):  
Katrin Zurfluh ◽  
Jochen Klumpp ◽  
Magdalena Nüesch-Inderbinen ◽  
Roger Stephan
Keyword(s):  
Escherichia Coli ◽  
Heavy Metal ◽  
Heavy Metal Resistance ◽  
Multidrug Resistant ◽  
Metal Resistance ◽  
Content Type ◽  
Esbl Gene ◽  
Extended Spectrum ◽  
Different Origins ◽  
First Time

ABSTRACTHere, we present the full sequences of threemcr-1-carrying plasmids isolated from extended-spectrum-β-lactamase (ESBL)-producingEscherichia coli. The plasmids belong to three different replicon types and are 34,640 bp, 209,401 bp, and 247,885 bp in size. We describe for the first time a composite transposon containingmcr-1localized on a multidrug-resistant (MDR) IncHI2 plasmid harboring additional determinants of resistance to six different classes of antibiotics, including the ESBL geneblaCTX-M-1, and heavy metal resistance.

scholarly journals Transcriptome Response to Heavy Metals in Sinorhizobium meliloti CCNWSX0020 Reveals New Metal Resistance Determinants That Also Promote Bioremediation by Medicago lupulina in Metal-Contaminated Soil

2017 ◽  
Vol 83 (20) ◽  
Author(s):  
Mingmei Lu ◽  
Shuo Jiao ◽  
Enting Gao ◽  
Xiuyong Song ◽  
Zhefei Li ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Contaminated Soils ◽  
Sinorhizobium Meliloti ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Content Type ◽  
Resistance Determinants ◽  
Medicago Lupulina ◽  
Legume Symbiosis ◽  
Cu And Zn

ABSTRACT The symbiosis of the highly metal-resistant Sinorhizobium meliloti CCNWSX0020 and Medicago lupulina has been considered an efficient tool for bioremediation of heavy metal-polluted soils. However, the metal resistance mechanisms of S. meliloti CCNWSX00200 have not been elucidated in detail. Here we employed a comparative transcriptome approach to analyze the defense mechanisms of S. meliloti CCNWSX00200 against Cu or Zn exposure. Six highly upregulated transcripts involved in Cu and Zn resistance were identified through deletion mutagenesis, including genes encoding a multicopper oxidase (CueO), an outer membrane protein (Omp), sulfite oxidoreductases (YedYZ), and three hypothetical proteins (a CusA-like protein, a FixH-like protein, and an unknown protein), and the corresponding mutant strains showed various degrees of sensitivity to multiple metals. The Cu-sensitive mutant (ΔcueO) and three mutants that were both Cu and Zn sensitive (ΔyedYZ, ΔcusA-like, and ΔfixH-like) were selected for further study of the effects of these metal resistance determinants on bioremediation. The results showed that inoculation with the ΔcueO mutant severely inhibited infection establishment and nodulation of M. lupulina under Cu stress, while inoculation with the ΔyedYZ and ΔfixH-like mutants decreased just the early infection frequency and nodulation under Cu and Zn stresses. In contrast, inoculation with the ΔcusA-like mutant almost led to loss of the symbiotic capacity of M. lupulina to even grow in uncontaminated soil. Moreover, the antioxidant enzyme activity and metal accumulation in roots of M. lupulina inoculated with all mutants were lower than those with the wild-type strain. These results suggest that heavy metal resistance determinants may promote bioremediation by directly or indirectly influencing formation of the rhizobium-legume symbiosis. IMPORTANCE Rhizobium-legume symbiosis has been promoted as an appropriate tool for bioremediation of heavy metal-contaminated soils. Considering the plant-growth-promoting traits and survival advantage of metal-resistant rhizobia in contaminated environments, more heavy metal-resistant rhizobia and genetically manipulated strains were investigated. In view of the genetic diversity of metal resistance determinants in rhizobia, their effects on phytoremediation by the rhizobium-legume symbiosis must be different and depend on their specific assigned functions. Our work provides a better understanding of the mechanism of heavy metal resistance determinants involved in the rhizobium-legume symbiosis, and in further studies, genetically modified rhizobia harboring effective heavy metal resistance determinants may be engineered for the practical application of rhizobium-legume symbiosis for bioremediation in metal-contaminated soils.

scholarly journals Coexistence of Heavy Metal and Antibiotic Resistance within a Novel Composite Staphylococcal Cassette Chromosome in a Staphylococcus haemolyticus Isolate from Bovine Mastitis Milk

2015 ◽  
Vol 59 (9) ◽  
pp. 5788-5792 ◽  
Author(s):  
Huping Xue ◽  
Zhaowei Wu ◽  
Longping Li ◽  
Fan Li ◽  
Yiqing Wang ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Antibiotic Resistance ◽  
Bovine Milk ◽  
Bovine Mastitis ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Coagulase Negative Staphylococci ◽  
Content Type ◽  
Staphylococcus Haemolyticus ◽  
Staphylococcal Cassette Chromosome

ABSTRACTThe structure of a composite staphylococcal cassette chromosome (SCC) carried by a methicillin-resistantStaphylococcus haemolyticus(NW19A) isolated from a bovine milk sample was analyzed. The formation of the circular forms of both single SCC elements and composite SCC elements was detected in NW19A. Twenty heavy metal and antibiotic resistance-related genes coexisted in this composite SCC, suggesting that these genes might be coselected under environmental pressure. Themecgene complex in NW19A, designated type C3, is different from classic C1 or C2 gene complexes structurally and likely evolves differently. Furthermore, results from alignment of the SCC composite island of NW19A with 50 related sequences from different staphylococcal strains provided additional evidence to support the notion that coagulase-negative staphylococci (CoNS) are the original host of heavy metal resistance genes among staphylococci. Given that a SCC composite island could transfer freely among different staphylococcal species from different hosts, more attention should be paid to contamination with heavy metals and antibiotics in dairy farming environments, including wastewater, soil, feces, and feed.

Sign in / Sign up

Export Citation Format

Share Document