scholarly journals Paenibacillus sp. strain UY79, isolated from a root nodule of Arachis villosa , displays a broad spectrum of antifungal activity

2021 ◽  
Author(s):  
Andrés Costa ◽  
Belén Corallo ◽  
Vanesa Amarelle ◽  
Silvina Stewart ◽  
Dinorah Pan ◽  
...  
Keyword(s):  
Volatile Compounds ◽  
Broad Spectrum ◽  
Growth Promotion ◽  
Pythium Ultimum ◽  
Macrophomina Phaseolina ◽  
Antagonistic Activity ◽  
Phytophthora Sojae ◽  
Whole Genome Sequence ◽  
Symbiotic Association ◽  
Paenibacillus Sp

A nodule-inhabiting Paenibacillus sp. strain (UY79) isolated from wild peanut ( Arachis villosa ) was screened for its antagonistic activity against diverse fungi and oomycetes ( Botrytis cinerea , Fusarium verticillioides , Fusarium oxysporum , Fusarium graminearum , Fusarium semitectum , Macrophomina phaseolina , Phomopsis longicolla , Pythium ultimum, Phytophthora sojae, Rhizoctonia solani , Sclerotium rolfsii and Trichoderma atroviride ). Results obtained show that Paenibacillus sp. UY79 was able to antagonize these fungi/oomycetes and that agar-diffusible compounds and volatile compounds (different from HCN), participate in the antagonism exerted. Acetoin, 2,3-butanediol and 2-methyl-1-butanol were identified among the volatile compounds produced by UY79 strain with possible antagonistic activity against fungi/oomycetes. Paenibacillus sp. strain UY79 did not affect symbiotic association or growth promotion of alfalfa plants when co-inoculated with rhizobia. By whole genome sequence analysis, we determined that strain UY79 is a new species of Paenibacillus within the Paenibacillus polymyxa complex. Diverse genes putatively involved in biocontrol activity were identified in the UY79 genome. Furthermore, according to genome mining and antibiosis assays, strain UY79 would have the capability to modulate the growth of bacteria commonly found in soil/plant communities. IMPORTANCE Phytopathogenic fungi and oomycetes are responsible for causing devastating losses in agricultural crops. Therefore, there is an enormous interest in the development of effective and complementary strategies that allow the control of the phytopathogens, reducing the input of agrochemicals in croplands. Discovery of new strains with expanded antifungal activities and with a broad spectrum of action is challenging and of great future impact. Diverse strains belonging to the P. polymyxa complex have been reported to be effective biocontrol agents. Results presented here show that the novel discovered strain of Paenibacillus sp. presents diverse traits involved in antagonistic activity against a broad spectrum of pathogens and would be a potential and valuable strain to be further assessed for the development of biofungicides.

scholarly journals Paenibacillus sp. strain UY79, isolated from a root nodule of Arachis villosa, displays a broad spectrum of antifungal activity

2021 ◽  
Author(s):  
Andrés Costa ◽  
Belén Corallo ◽  
Vanesa Amarelle ◽  
Silvina Stewart ◽  
Dinorah Pan ◽  
...  
Keyword(s):  
Growth Promotion ◽  
Fusarium Verticillioides ◽  
Genome Mining ◽  
Sclerotium Rolfsii ◽  
Pythium Ultimum ◽  
Macrophomina Phaseolina ◽  
Phytophthora Sojae ◽  
Whole Genome Sequence ◽  
Symbiotic Association ◽  
Paenibacillus Sp

A nodule-inhabiting Paenibacillus sp. strain (UY79) isolated from wild peanut (Arachis villosa) was screened for its antagonistic activity against diverse fungi and oomycetes (Botrytis cinerea, Fusarium verticillioides, Fusarium oxysporum, Fusarium graminearum, Fusarium semitectum, Macrophomina phaseolina, Phomopsis longicolla, Pythium ultimum, Phytophthora sojae, Rhizoctonia solani, Sclerotium rolfsii and Trichoderma atroviride). Results obtained show that Paenibacillus sp. UY79 was able to antagonize these fungi/oomycetes and that agar-diffusible metabolites and enzymes, as well as volatile compounds (different from HCN), participate in the antagonism exerted. We found that Paenibacillus sp. strain UY79 did not affect symbiotic association or growth promotion of alfalfa plants when co-inoculated with rhizobia. By whole genome sequence analysis, we determined that strain UY79 is a new species of Paenibacillus within the Paenibacilluspolymyxa complex. Diverse genes putatively involved in biocontrol activity were identified in the UY79 genome. Moreover, according to genome mining and antibiosis assays, strain UY79 would have the capability to modulate the growth of bacteria commonly found in soil/plant communities.

scholarly journals ANTAGONISTIC ACTIVITY AND PLANT GROWTH PROMOTING RHIZOBACTERIA ISOLATED FROM FOREST PLANT RHIZOSPHERE AGAINST FUSARIUM SOLANI ON THUJA SEEDLINGS

2021 ◽  
Vol 52 (6) ◽  
pp. 1508-1515
Author(s):  
B. Yasin ◽  
O. Omer Ali ◽  
T. Sulaiman Rashid
Keyword(s):  
Plant Growth ◽  
Fusarium Solani ◽  
Untreated Control ◽  
Growth Promotion ◽  
Plant Growth Promoting Rhizobacteria ◽  
Good Health ◽  
Antagonistic Activity ◽  
Pseudomonas Sp ◽  
Chlorophyll Contents ◽  
Paenibacillus Sp

Root diseases are one of the main forest nursery problems that have a significant impact on forest production which are caused by Fusarium solani. Rhizobacteria from healthy forest soils were isolated and screened in streak method to select antagonistic strains against F. solani. Two isolates showed high antagonistic activity and molecularly identified as Paenibacillus sp. and Pseudomonas sp. The capability of the Paenibacillus sp. and Pseudomonas sp. were tested in greenhouse plastic containers experiments against F. solani. Soil bacterization with Paenibacillus sp. and Pseudomonas sp. significantly protected thuja seedlings from F. solani compared to the untreated control seedlings. The containers added by Paenibacillus sp. and pseudomonas sp. are also showed plant growth promotion including shoot length, root length, dry and wet weights of the seedlings as well as the chlorophyll contents of the thuja seedlings compared to the untreated control plants. In this research it has been showing that the rhizobacterial treatments have potential to decrease the effect of fungal disease severity, promoting the plant growth and also helps plants to maintain a good health. 

scholarly journals BIOTECHNOLOGICAL POTENTIAL OF SOYBEAN PLANT GROWTH-PROMOTING RHIZOBACTERIA

2021 ◽  
Vol 34 (2) ◽  
pp. 328-338
Author(s):  
GABRIEL FERREIRA DE PAULA ◽  
GILBERTO BUENO DEMÉTRIO ◽  
LEOPOLDO SUSSUMU MATSUMOTO
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Plant Growth Promoting Rhizobacteria ◽  
Macrophomina Phaseolina ◽  
Phytopathogenic Fungi ◽  
Antagonistic Activity ◽  
Soybean Plant ◽  
Bacterial Isolates ◽  
Iaa Production ◽  
Soybean Plants

ABSTRACT Technologies that use rhizobacteria to promote plant growth are increasing in agriculture, results have shown improvements in soil quality, increases in productivity, and decreases in the use of synthetic inputs, The objective of work was to characterize bacterial isolates regarding their biological activity and growth promotion of soybean plants grown in a controlled environment. Fifteen bacteria were isolated from soils with continuous use of biological fertilizer. They were evaluated for enzymes production (amylase and protease), nitrogen fixation, antagonistic activity to phytopathogenic fungi, and indoleacetic acid (IAA) production, Soybean seeds were inoculated with bacterial isolates in a greenhouse and evaluated for plant development and soil chemical attributes. The results showed that 8 of the 15 isolates presented production of amylase, protease, or both and 4 isolates presented nitrogen-fixing capacity. The percentage of isolates with high or moderate inhibitory action against the fungi Sclerotinia sclerotiorum, Macrophomina phaseolina, and Fusarium solani were 73.3%, 66.6%, and 73.3%, respectively. The IAA production varied from 8.56 to 31.33 µg mL-1 (5 isolates had low, 6 had moderate, and 4 had high production). The soybean development was significantly higher in 80% of the treatments with inoculation with bacterial isolates. Five bacterial isolates effectively present all characteristics for use as inoculant (biofertilizer) to promote the development of soybean plants.

Effect of the endophytic plant growth promoting Enterobacter ludwigii EB4B on tomato growth

2020 ◽  
Vol 13 (2) ◽  
pp. 54-65 ◽  
Author(s):  
M.E.A. Bendaha ◽  
H.A. Belaouni
Keyword(s):  
Root Length ◽  
Biocontrol Agent ◽  
Growth Promotion ◽  
Dry Weight ◽  
Antagonistic Activity ◽  
Rrna Gene ◽  
Plant Growth Promoting ◽  
Enterobacter Ludwigii ◽  
Tomato Growth

SummaryThis study aims to develop a biocontrol agent against Fusarium oxysporum f.sp. radicis-lycopersici (FORL) in tomato. For this, a set of 23 bacterial endophytic isolates has been screened for their ability to inhibit in vitro the growth of FORL using the dual plate assay. Three isolates with the most sound antagonistic activity to FORL have been qualitatively screened for siderophore production, phosphates solubilization and indolic acetic acid (IAA) synthesis as growth promotion traits. Antagonistic values of the three candidates against FORL were respectively: 51.51 % (EB4B), 51.18 % (EB22K) and 41.40 % (EB2A). Based on 16S rRNA gene sequence analysis, the isolates EB4B and EB22K were closely related to Enterobacter ludwigii EN-119, while the strain EB2A has been assigned to Leclercia adecarboxylata NBRC 102595. The promotion of tomato growth has been assessed in vitro using the strains EB2A, EB4B and EB22K in presence of the phytopathogen FORL. The treatments with the selected isolates increased significantly the root length and dry weight. Best results were observed in isolate EB4B in terms of growth promotion in the absence of FORL, improving 326.60 % of the root length and 142.70 % of plant dry weight if compared with untreated controls. In the presence of FORL, the strain EB4B improved both root length (180.81 %) and plant dry weight (202.15 %). These results encourage further characterization of the observed beneficial effect of Enterobacter sp. EB4B for a possible use as biofertilizer and biocontrol agent against FORL.

scholarly journals C4 Bacterial Volatiles Improve Plant Health

Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 682
Author(s):  
Bruno Henrique Silva Dias ◽  
Sung-Hee Jung ◽  
Juliana Velasco de Castro Oliveira ◽  
Choong-Min Ryu
Keyword(s):  
Plant Growth ◽  
Volatile Compounds ◽  
Large Scale ◽  
Growth Promotion ◽  
Plant Growth Promoting Rhizobacteria ◽  
Plant Health ◽  
Systemic Resistance ◽  
Potential Applications ◽  
Plant Growth Promoting ◽  
Bacterial Volatiles

Plant growth-promoting rhizobacteria (PGPR) associated with plant roots can trigger plant growth promotion and induced systemic resistance. Several bacterial determinants including cell-wall components and secreted compounds have been identified to date. Here, we review a group of low-molecular-weight volatile compounds released by PGPR, which improve plant health, mostly by protecting plants against pathogen attack under greenhouse and field conditions. We particularly focus on C4 bacterial volatile compounds (BVCs), such as 2,3-butanediol and acetoin, which have been shown to activate the plant immune response and to promote plant growth at the molecular level as well as in large-scale field applications. We also disc/ uss the potential applications, metabolic engineering, and large-scale fermentation of C4 BVCs. The C4 bacterial volatiles act as airborne signals and therefore represent a new type of biocontrol agent. Further advances in the encapsulation procedure, together with the development of standards and guidelines, will promote the application of C4 volatiles in the field.

Identification of three closely linked loci conferring broad-spectrum Phytophthora sojae resistance in soybean variety Tosan-231

2021 ◽  
Author(s):  
Jun-ichi Matsuoka ◽  
Mami Takahashi ◽  
Tetsuya Yamada ◽  
Yuhi Kono ◽  
Naohiro Yamada ◽  
...  
Keyword(s):  
Broad Spectrum ◽  
Phytophthora Sojae ◽  
Soybean Variety

scholarly journals Genetic Mapping and Molecular Characterization of a Broad-spectrum Phytophthora sojae Resistance Gene in Chinese Soybean

2019 ◽  
Vol 20 (8) ◽  
pp. 1809 ◽  
Author(s):  
Chao Zhong ◽  
Yinping Li ◽  
Suli Sun ◽  
Canxing Duan ◽  
Zhendong Zhu
Keyword(s):  
Genetic Mapping ◽  
Candidate Gene ◽  
Broad Spectrum ◽  
Genomic Region ◽  
Phytophthora Sojae ◽  
Genetic Population ◽  
Phytophthora Root Rot ◽  
Different Origins ◽  
Lrr Domain

Phytophthora root rot (PRR) causes serious annual soybean yield losses worldwide. The most effective method to prevent PRR involves growing cultivars that possess genes conferring resistance to Phytophthora sojae (Rps). In this study, QTL-sequencing combined with genetic mapping was used to identify RpsX in soybean cultivar Xiu94-11 resistance to all P. sojae isolates tested, exhibiting broad-spectrum PRR resistance. Subsequent analysis revealed RpsX was located in the 242-kb genomic region spanning the RpsQ locus. However, a phylogenetic investigation indicated Xiu94-11 carrying RpsX is distantly related to the cultivars containing RpsQ, implying RpsX and RpsQ have different origins. An examination of candidate genes revealed RpsX and RpsQ share common nonsynonymous SNP and a 144-bp insertion in the Glyma.03g027200 sequence encoding a leucine-rich repeat (LRR) region. Glyma.03g027200 was considered to be the likely candidate gene of RpsQ and RpsX. Sequence analyses confirmed that the 144-bp insertion caused by an unequal exchange resulted in two additional LRR-encoding fragments in the candidate gene. A marker developed based on the 144-bp insertion was used to analyze the genetic population and germplasm, and proved to be useful for identifying the RpsX and RpsQ alleles. This study implies that the number of LRR units in the LRR domain may be important for PRR resistance in soybean.

scholarly journals Response of Soybean Pathogens to Glyceollin

2010 ◽  
Vol 100 (9) ◽  
pp. 897-903 ◽  
Author(s):  
Anatoly V. Lygin ◽  
Curtis B. Hill ◽  
Olga V. Zernova ◽  
Laura Crull ◽  
Jack M. Widholm ◽  
...  
Keyword(s):  
Hairy Root ◽  
Degradation Products ◽  
Macrophomina Phaseolina ◽  
Phytophthora Sojae ◽  
Plant Diseases ◽  
Phialophora Gregata ◽  
Diaporthe Phaseolorum ◽  
Significant Difference ◽  
Transformed Hairy Root ◽  
Soybean Genotypes

Plants recognize invading pathogens and respond biochemically to prevent invasion or inhibit colonization in plant cells. Enhancing this response in crop plants could improve sustainable methods to manage plant diseases. To enhance disease resistance in soybean, the soybean phytoalexin glyceollin was assessed in soybean hairy roots of two soybean genotypes, Spencer and PI 567374, transformed with either soybean isoflavone synthase (IFS2) or chalcone synthase (CHS6) genes that were inoculated with the soybean pathogens Diaporthe phaseolorum var. meridionales, Macrophomina phaseolina, Sclerotinia sclerotiorum, and Phytophthora sojae. The hairy-root-transformed lines had several-fold decreased levels of isoflavone daidzein, the precursor of glyceollin, and considerably lower concentrations of glyceollin induced by pathogens measured 5 days after fungal inoculation compared with the nontransformed controls without phenolic transgenes. M. phaseolina, P. sojae, and S. sclerotiorum grew much more on IFS2- and CHS6-transformed roots than on control roots, although there was no significant difference in growth of D. phaseolorum var. meridionales on the transformed hairy-root lines. In addition, glyceollin concentration was lower in D. phaseolorum var. meridionales-inoculated transformed and control roots than roots inoculated with the other pathogens. Glyceollin inhibited the growth of D. phaseolorum var. meridionales, M. phaseolina, P. sojae, S. sclerotiorum, and three additional soybean pathogens: Cercospora sojina, Phialophora gregata, and Rhizoctonia solani. The most common product of glyceollin conversion or degradation by the pathogens, with the exception of P. sojae, which had no glyceollin degradation products found in the culture medium, was 7-hydroxyglyceollin.

scholarly journals ​Antagonistic Activity of Panchagavya and Trichoderma spp. against Wilt Complex causing Pathogens of Chickpea (Cicer arietinum L.)

2022 ◽  
Author(s):  
H.V. Parmar ◽  
N.M. Gohel
Keyword(s):  
Low Cost ◽  
Trichoderma Viride ◽  
Macrophomina Phaseolina ◽  
Antagonistic Activity ◽  
Culture Technique ◽  
Plant Diseases ◽  
Dual Culture ◽  
Trichoderma Spp ◽  
Soil Borne Pathogens ◽  
Chickpea Wilt

Background: Chickpea wilt complex caused by several soil-borne pathogens is the major yield-reducing malady worldwide. Biological control is one of the best, low-cost and ecologically sustainable method for managing plant diseases caused by soil-borne pathogens. Methods: In this present investigation Panchagavya and Trichoderma spp. were evaluated by following poisoned food technique and dual culture technique against wilt complex causing pathogens i.e. Fusarium oxysporum f. sp. ciceri, Fusarium solani and Macrophomina phaseolina. Result: Among the different isolates of Trichoderma spp. evaluated, Trichoderma viride (AAU isolate) was highly antagonistic to F. oxysporum f. sp. ciceri (52.78%) and F. solani (65.37%) whereas, Trichoderma asperellum (AAU isolate) was highly antagonistic to M. phaseolina (65.93%). Panchagavya at the highest concentration (50%) showed significantly higher efficacy (80.74, 66.62 and 49.67%) in inhibiting the mycelial growth of all three pathogens and at the lowest concentration it was moderately effective.

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