scholarly journals Waterborne Viruses and F-Specific Coliphages in Mixed-Use Watersheds: Microbial Associations, Host Specificities, and Affinities with Environmental/Land Use Factors

2016 ◽  
Vol 83 (3) ◽  
Author(s):  
Tineke H. Jones ◽  
Julie Brassard ◽  
Edward Topp ◽  
Graham Wilkes ◽  
David R. Lapen
Keyword(s):  
Land Use ◽  
Surface Water ◽  
Surface Waters ◽  
Water Samples ◽  
Land Development ◽  
Hepatitis E ◽  
Surface Water Flow ◽  
Mixed Use ◽  
Human Viruses ◽  
Waterborne Viruses

ABSTRACT From the years 2008 to 2014, a total of 1,155 water samples were collected (spring to fall) from 24 surface water sampling sites located in a mixed-used but predominantly agricultural (i.e., dairy livestock production) river basin in eastern Ontario, Canada. Water was analyzed for viable F-specific DNA (F-DNA) and F-specific RNA (F-RNA) (genogroup I [GI] to GIV) coliphage and a suite of molecularly detected viruses (norovirus [GI to GIV], torque teno virus [TTV], rotavirus, kobuvirus, adenovirus, astrovirus, hepatitis A, and hepatitis E). F-DNA and F-RNA coliphage were detected in 33 and 28% of the samples at maximum concentrations of 2,000 and 16,300 PFU · 100 ml−1, respectively. Animal TTV, human TTV, kobuvirus, astrovirus, and norovirus GIII were the most prevalent viruses, found in 23, 20, 13, 12, and 11% of samples, respectively. Viable F-DNA coliphage was found to be a modest positive indicator of molecularly detected TTV. F-RNA coliphage, unlike F-DNA coliphage, was a modest positive predictor of norovirus and rotavirus. There were, however, a number of significant negative associations among F-specific coliphage and viruses. F-DNA coliphage densities of >142 PFU · 100 ml−1 delineated conditions when ∼95% of water samples contained some type of virus. Kobuvirus was the virus most strongly related to detection of any other virus. Land use had some associations with virus/F-specific coliphage detection, but season and surface water flow were the variables that were most important for broadly delineating detection. Higher relative levels of detection of human viruses and human F-RNA coliphage were associated with higher relative degrees of upstream human land development in a catchment. IMPORTANCE This study is one of the first, to our knowledge, to evaluate relationships among F-specific coliphages and a large suite of enteric viruses in mixed-use but agriculturally dominated surface waters in Canada. This study suggested that relationships between viable F-specific coliphages and molecularly detected viruses do exist, but they are not always positive. Caution should be employed if viable F-specific coliphages are to be used as indicators of virus presence in surface waters. This study elucidates relative effects of agriculture, wildlife, and human activity on virus and F-specific coliphage detection. Seasonal and meteorological attributes play a strong role in the detection of most virus and F-specific coliphage targets.

scholarly journals Fast UHPLC-MS/MS for the Simultaneous Determination of Azithromycin, Erythromycin, Fluoxetine and Sotalol in Surface Water Samples

2021 ◽  
Vol 11 (18) ◽  
pp. 8316
Author(s):  
Mira Azzi ◽  
Sylvain Ravier ◽  
Assem Elkak ◽  
Bruno Coulomb ◽  
Jean-Luc Boudenne
Keyword(s):  
Surface Water ◽  
Simultaneous Determination ◽  
Surface Waters ◽  
Water Samples ◽  
High Performance ◽  
Chemical Properties ◽  
Environmental Water ◽  
Butyl Ether ◽  
Flight Mass Spectrometry

Chromatographic development for the determination of pharmaceuticals in environmental water samples is particularly challenging when the analytes have significantly different physico-chemical properties (solubility, polarity, pKa) often requiring multiple chromatographic methods for each active component. This paper presents a method for the simultaneous determination of azithromycin, erythromycin (antibiotics), fluoxetine (anti-depressant) and sotalol (b-blocker) in surface waters by ultra-high-performance liquid chromatography coupled with ultra-high-resolution time-of-flight mass spectrometry. These pharmaceuticals—presenting a broad spectrum of polarity (0.24 ≤ log Kow ≤ 4.05)—were separated on a C-18 analytical column, after a simple filtration step for freshwater samples or after a liquid–liquid extraction with Methyl-tertio-butyl ether (MTBE) for seawater samples. The optimized separation method (in terms of nature of column and eluent, elution gradient, and of mass spectrometric parameters), enable one to reach limits of detection ranging between 2 and 7 ng L−1 and limits of quantification between 7 and 23 ng L−1 for the four targeted molecules, within a three minute run. This method was validated using samples collected from three different surface waters in Lebanon (freshwater and seawater) and analytical results were compared with those obtained in surface waters sampled in a French river, equivalent in terms of human activities. Using this method, we report the highest concentration of pharmaceuticals found in surface water (up to 377 ng L−1 and 268 ng L−1, respectively, for azithromycin and erythromycin, in the Litani river, Lebanon).

scholarly journals Concentration and Detection of Cryptosporidium Oocysts in Surface Water Samples by Method 1622 Using Ultrafiltration and Capsule Filtration

2001 ◽  
Vol 67 (3) ◽  
pp. 1123-1127 ◽  
Author(s):  
Otto D. Simmons ◽  
Mark D. Sobsey ◽  
Christopher D. Heaney ◽  
Frank W. Schaefer ◽  
Donna S. Francy
Keyword(s):  
Surface Water ◽  
Surface Waters ◽  
Hollow Fiber ◽  
Water Samples ◽  
Environmental Protection Agency ◽  
Fluorescent Antibody ◽  
Immunomagnetic Separation ◽  
Recovery Rates ◽  
Microscopic Evaluation ◽  
Surface Water Samples

ABSTRACT The protozoan parasite Cryptosporidium parvumis known to occur widely in both source and drinking water and has caused waterborne outbreaks of gastroenteritis. To improve monitoring, the U.S. Environmental Protection Agency developed method 1622 for isolation and detection of Cryptosporidium oocysts in water. Method 1622 is performance based and involves filtration, concentration, immunomagnetic separation, fluorescent-antibody staining and 4′,6-diamidino-2-phenylindole (DAPI) counterstaining, and microscopic evaluation. The capsule filter system currently recommended for method 1622 was compared to a hollow-fiber ultrafilter system for primary concentration of C. parvum oocysts in seeded reagent water and untreated surface waters. Samples were otherwise processed according to method 1622. Rates of C. parvumoocyst recovery from seeded 10-liter volumes of reagent water in precision and recovery experiments with filter pairs were 42% (standard deviation [SD], 24%) and 46% (SD, 18%) for hollow-fiber ultrafilters and capsule filters, respectively. Mean oocyst recovery rates in experiments testing both filters on seeded surface water samples were 42% (SD, 27%) and 15% (SD, 12%) for hollow-fiber ultrafilters and capsule filters, respectively. Although C. parvum oocysts were recovered from surface waters by using the approved filter of method 1622, the recovery rates were significantly lower and more variable than those from reagent grade water. In contrast, the disposable hollow-fiber ultrafilter system was compatible with subsequent method 1622 processing steps, and it recovered C. parvum oocysts from seeded surface waters with significantly greater efficiency and reliability than the filter suggested for use in the version of method 1622 tested.

scholarly journals Occurrence and Distribution of Pharmaceuticals and their Transformation Products in Luxembourgish Surface Waters

2021 ◽  
Author(s):  
Randolph Singh ◽  
Adelene Lai ◽  
Jessy Krier ◽  
Todor Kondić ◽  
Philippe Diderich ◽  
...  
Keyword(s):  
Surface Water ◽  
Surface Waters ◽  
Water Samples ◽  
Transformation Products ◽  
Computational Tools ◽  
Data Files ◽  
Surface Water Samples

<p>This pre-print describes the analysis of pharmaceuticals and their transformation products in surface water samples collected in Luxembourg from 2019 to 2020. Details of the experimental and computational tools and workflows used are fully described in the manuscript. Links to the suspect lists, codes used, and data files are also provided.</p>

scholarly journals DISTRIBUTION OF CHEMICAL ELEMENTS IN SURFACE WATERS FROM THE CRNA RIVER BASIN, REPUBLIC OF MACEDONIA

2018 ◽  
Vol 39 (1) ◽  
pp. 31 ◽  
Author(s):  
Dimitri Tomovski ◽  
Trajče Stafilov ◽  
Robert Šajn ◽  
Katerina Bačeva Andonovska
Keyword(s):  
Surface Water ◽  
Surface Waters ◽  
Water Samples ◽  
Inductively Coupled Plasma ◽  
Chemical Elements ◽  
Emission Spectrometry ◽  
Anthropogenic Influence ◽  
Mean Values ◽  
Republic Of Macedonia ◽  
Surface Water Samples

An investigation of the distribution of 23 chemical elements (Ag, Al, As, B, Ba, Ca, Cd, Co, Cr, Cu, Fe, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, Sr, V and Zn) in surface water samples from the entire basin of the Crna River, Republic of Macedonia, was carried out. In total 31 water samples were collected, from which 8 samples from the Crna River and 4 samples from four main tributaries of Crna River in the Pelagonia Valley (Blato, Prilepska Reka, Dragor and Jelaška Reka). Also, surface water samples were collected from 3 locations in the Tikveš Lake, 8 locations from the Majdanska River and river of Blašnica before its inflow into the Tikveš Lake and from 7 locations of the lower course of the Crna River after the dam of Tikveš Lake until its inflow into the river of Vardar. Determination of the concen-tration of the investigated elements was performed by using atomic emission spectrometry with inductively coupled plasma (ICP-AES). All data obtained for the analyzed samples were statistically processed using software Stat Soft 11.0 where the descriptive statistical analysis of the value for the concentration of the elements was performed. The maps of spatial distribution of the concentration for each element and a histograms for the representation of elements with mean values of the concentrations by regions, were also prepared. The obtained results show that the concentration of investigated element are mainly followed the lithology of the region. However, higher concentrations of arsenic were found in the water samples from the river of Blašnica which is a result of anthropogenic influence from the abounded Allchar mine on the Kožuf Mountain. Also, the increased concentrations of nickel were found in the samples from the lower couse of the Crna River after the dam of Tikveš Lake due to the anthropogenic influence from the ferronickel smelter plant Feni Industry, which can influence the quality parameters of surface waters.

scholarly journals Chemical Quality of Surface Water of Bhola Sadar Upazila in Bangladesh for Irrigation, Aquaculture, Drinking and Livestock Consumption

2015 ◽  
Vol 6 (1) ◽  
pp. 131-140
Author(s):  
MF Karim ◽  
MW Zaman ◽  
R Sultana ◽  
MU Nizam ◽  
MR Kamruzzaman
Keyword(s):  
Surface Water ◽  
Surface Waters ◽  
Water Samples ◽  
Pond Water ◽  
Magnesium Content ◽  
Low Salinity ◽  
Quality Of Water ◽  
Hard Limit ◽  
Surface Water Samples

A study was carried out with 25 pond water samples of Bhola Sadar Upazila to assess the quality of surface water for irrigation, aquaculture, drinking and livestock consumption. Chemical analyses of different parameters were done to assess the quality of water. All of the water samples showed slightly acidic in nature. Sixteen surface water samples were not suitable for drinking and aquaculture in respect of pH (pH <6.5). Electrical conductivity (EC) categorized the waters as “low salinity” (C1) to “medium salinity” (C2) class for irrigation. With respect to total dissolved solids surface waters were within “highest desirable limit” for drinking and irrigation and suitable for livestock consumption and aquaculture. Calcium and Magnesium content rated the samples as “maximum permissible” and “highest desirable” limit for drinking. All the samples were suitable for drinking in case of Na and K, 21 samples were not suitable for aquaculture due to higher (>5.0 mg L-1) K content. Six samples were unsuitable for livestock due to higher (Cl >30mgL- 1) Cl values. SSP rated 9 samples as “good”, 1 as “excellent”, 6 as “doubtful” and 9 as “permissible” for irrigation. With respect to RSC 21 samples were “suitable”, 3 were “marginal” and 1 was “unsuitable” for irrigation. Hardness classified 14 samples within “moderately hard”, 10 within “soft” and only one as “hard” limit for irrigation and 1 sample (No. 16) was unsuitable for livestock consumption. P, B, Cu and As concentration categorized all the samples suitable for irrigation, aquaculture, drinking and livestock consumption.DOI: http://dx.doi.org/10.3329/jesnr.v6i1.22053 J. Environ. Sci. & Natural Resources, 6(1): 131-140 2013

scholarly journals Spatiotemporal Analysis of Cryptosporidium Species/Genotypes and Relationships with Other Zoonotic Pathogens in Surface Water from Mixed-Use Watersheds

2012 ◽  
Vol 79 (2) ◽  
pp. 434-448 ◽  
Author(s):  
Graham Wilkes ◽  
Norma J. Ruecker ◽  
Norman F. Neumann ◽  
Victor P. J. Gannon ◽  
Cassandra Jokinen ◽  
...  
Keyword(s):  
Land Use ◽  
Surface Water ◽  
Water Samples ◽  
Regression Tree ◽  
Infection Risk ◽  
Human Infection ◽  
Classification And Regression Tree ◽  
Odds Ratios ◽  
Content Type ◽  
Zoonotic Pathogens

ABSTRACTNearly 690 raw surface water samples were collected during a 6-year period from multiple watersheds in the South Nation River basin, Ontario, Canada.Cryptosporidiumoocysts in water samples were enumerated, sequenced, and genotyped by detailed phylogenetic analysis. The resulting species and genotypes were assigned to broad, known host and human infection risk classes. Wildlife/unknown, livestock, avian, and human host classes occurred in 21, 13, 3, and <1% of sampled surface waters, respectively.Cryptosporidium andersoniwas the most commonly detected livestock species, while muskrat I and II genotypes were the most dominant wildlife genotypes. The presence ofGiardiaspp.,Salmonellaspp.,Campylobacterspp., andEscherichia coliO157:H7 was evaluated in all water samples. The greatest significant odds ratios (odds of pathogen presence when host class is present/odds of pathogen presence when host class is absent) forGiardiaspp.,Campylobacterspp., andSalmonellaspp. in water were associated, respectively, with livestock (odds ratio of 3.1), avian (4.3), and livestock (9.3) host classes. Classification and regression tree analyses (CART) were used to group generalized host and human infection risk classes on the basis of a broad range of environmental and land use variables while tracking cooccurrence of zoonotic pathogens in these groupings. The occurrence of livestock-associatedCryptosporidiumwas most strongly related to agricultural water pollution in the fall (conditions also associated with elevated odds ratios of other zoonotic pathogens occurring in water in relation to all sampling conditions), whereas wildlife/unknown sources ofCryptosporidiumwere geospatially associated with smaller watercourses where urban/rural development was relatively lower. Conditions that support wildlife may not necessarily increase overall human infection risks associated withCryptosporidiumsince mostCryptosporidiumgenotypes classed as wildlife in this study (e.g., muskrat I and II genotype) do not pose significant infection risks to humans. Consequently, from a human health perspective, land use practices in agricultural watersheds that create opportunities for wildlife to flourish should not be rejected solely on the basis of their potential to increase relative proportions of wildlife fecal contamination in surface water. The present study suggests that mitigating livestock fecal pollution in surface water in this region would likely reduce human infection risks associated withCryptosporidiumand other zoonotic pathogens.

scholarly journals Spatio-Temporal Analysis of Surface Water Quality in Mokopane Area, Limpopo, South Africa

Water ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 220
Author(s):  
Mmasabata Dolly Molekoa ◽  
Ram Avtar ◽  
Pankaj Kumar ◽  
Huynh Vuong Thu Minh ◽  
Rajarshi Dasgupta ◽  
...  
Keyword(s):  
South Africa ◽  
Water Quality ◽  
Heavy Metal ◽  
Land Use ◽  
Surface Water ◽  
Water Samples ◽  
Surface Water Quality ◽  
Quality Data ◽  
Chemical Parameters ◽  
Physico Chemical

Considering the well-documented impacts of land-use change on water resources and the rapid land-use conversions occurring throughout Africa, in this study, we conducted a spatiotemporal analysis of surface water quality and its relation with the land use and land cover (LULC) pattern in Mokopane, Limpopo province of South Africa. Various physico-chemical parameters were analyzed for surface water samples collected from five sampling locations from 2016 to 2020. Time-series analysis of key surface water quality parameters was performed to identify the essential hydrological processes governing water quality. The analyzed water quality data were also used to calculate the heavy metal pollution index (HPI), heavy metal evaluation index (HEI) and weighted water quality index (WQI). Also, the spatial trend of water quality is compared with LULC changes from 2015 to 2020. Results revealed that the concentration of most of the physico-chemical parameters in the water samples was beyond the World Health Organization (WHO) adopted permissible limit, except for a few parameters in some locations. Based on the calculated values of HPI and HEI, water quality samples were categorized as low to moderately polluted water bodies, whereas all water samples fell under the poor category (>100) and beyond based on the calculated WQI. Looking precisely at the water quality’s temporal trend, it is found that most of the sampling shows a deteriorating trend from 2016 to 2019. However, the year 2020 shows a slightly improving trend on water quality, which can be justified by lowering human activities during the lockdown period imposed by COVID-19. Land use has a significant relationship with surface water quality, and it was evident that built-up land had a more significant negative impact on water quality than the other land use classes. Both natural processes (rock weathering) and anthropogenic activities (wastewater discharge, industrial activities etc.) were found to be playing a vital role in water quality evolution. This study suggests that continuous assessment and monitoring of the spatial and temporal variability of water quality in Limpopo is important to control pollution and health safety in the future.

scholarly journals Viable Putative Vibrio vulnificus and parahaemolyticus in the Pensacola and Perdido Bays: Water Column, Sediments, and Invertebrate Biofilms

2021 ◽  
Vol 8 ◽  
Author(s):  
Trupti V. Potdukhe ◽  
Jane M. Caffrey ◽  
Mackenzie J. Rothfus ◽  
Carrie E. Daniel ◽  
Michael E. Swords ◽  
...  
Keyword(s):  
Surface Water ◽  
Surface Waters ◽  
Water Samples ◽  
Bottom Water ◽  
Vibrio Vulnificus ◽  
General Term ◽  
Salinity Stratification ◽  
Sediment Samples ◽  
Wind Speeds ◽  
Water Ph

Vibriosis is the general term for human illnesses caused by infection of pathogenic Vibrio species. Vibrio vulnificus (Vv) and parahaemolyticus (Vp) are two problematic waterborne pathogens that have yet to be enumerated in northwest Florida coastal Gulf of Mexico estuaries. In this regionally novel study, we surveyed 43 locations in two subtropical estuarine systems, Perdido Bay and Pensacola Bay, over seven dates in winter 2020. Sampling included three substrate types: surface waters, sediments, and invertebrate biofilms. We determined baseline abundances of presumptive viable Vv and Vp appearing as colonies on CHROMagar (Vv, blue; Vp, purple). Vv was detected in 37 out of 43 water samples, with maximum levels of 3,556 CFU/mL. Vp was only detected in 15 water samples, with a maximum concentration of 8,919 CFU/mL. Sediments contained Vv in all but one sample, with concentrations ranging from 121 to 607,222 CFU/mL. In contrast, Vp were only detected in 33 sediment samples, where concentrations ranged from 28 to 77,333 CFU/mL. Opportunistically-sampled surface swabs (biofilms), collected from shells (either oyster or barnacle) and polychaete worms found in sediment samples, contained on average 7,735 and 1,490 CFU/mL of Vv and Vp, respectively. Surface water Vv abundances covaried with bottom water pH, maximum prior cumulative wind speeds, and tidal coefficient on the day of sampling. Vp surface water abundances negatively correlated with surface water salinity, surface water pH, and bottom water pH and positively correlated with total surface dissolved inorganic and total Kjeldahl nitrogen concentrations, and wind. Spatially, there was large variation in Vibrio densities in surface waters; abundances of both species were strongly correlated with wind, suggesting resuspension was important. Sedimentary abundances of both putative Vv and Vp shared a correlation with one parameter: salinity stratification. Due to the length of this study, temperature was not considered a major factor. This short-term (1 month) study was designed not to enumerate pathogenic Vv or Vp, but rather to establish the first winter baseline of Vibrio abundances for this region. Determination of these baseline winter cultivable putative Vibrio abundances will be valuable in predicting relative risk factors in each waterbody of interest.

scholarly journals Nitrate Trends During the Seasons on Bahlui River and its Tributaries in Build-Up Area of Iasi City

2011 ◽  
Vol 68 (2) ◽  
Author(s):  
Ana Maria OIŞTE ◽  
Iuliana Gabriela
Keyword(s):  
Land Use ◽  
Surface Water ◽  
Surface Waters ◽  
Point Sources ◽  
Chemical Parameters ◽  
Nitrate Concentrations ◽  
Arc Gis ◽  
Agricultural Areas ◽  
Gis Software ◽  
The City

Nitrates are natural components of the surface water, the amounts in surface waters is very important, usually being insignificant, but in urban area and along agricultural areas situated inside the river basin, their concentration is increased, being non-point sources and point sources located in the city. The nitrate concentrations is influenced both by the seasonal changes of rainfall and temperature as well as by land-use transformations, the variation being closely related to them. This paper presents the nitrate trends during seasons, the samples was taken in December, April and June from 76 points, the influence of the season characteristics being obvious. Obtained data indicates an upward trend, so that nitrate concentrations increase from December to June, higher values registered on tributaries of Bahlui river, caused by of the land-use, smaller flow and seasons variation of other physic-chemical parameters, otherwise the synergistic action of the natural and anthropic sources and parameters. Arc GIS software was used for illustrate the results followed by a statistics software were used to establish some correlations based on multivariate analysis. The analysis shows that non-point and anthropic sources of nitrates, influence nitrate regime during the succession of the seasons, nitrate levels almost doubled in surface water, or even higher in Bahlui river and its tributaries.

Neonicotinoids Detection by new LC-MS/MS Method in Romanian Surface Waters

2017 ◽  
Vol 68 (8) ◽  
pp. 1716-1722 ◽  
Author(s):  
Vasile Ion Iancu ◽  
Toma Galaon ◽  
Marcela Niculescu ◽  
Carol Blaziu Lehr
Keyword(s):  
Surface Water ◽  
Surface Waters ◽  
Water Samples ◽  
Solid Phase ◽  
Monitoring Program ◽  
Danube River ◽  
Limit Of Quantification ◽  
Neonicotinoid Insecticides ◽  
Relative Standard ◽  
Surface Water Samples

Increasing and widespread use of neonicotinoid insecticides in all world, together with their highly toxicity to invertebrates and environmental persistence mean that surface waters need to be monitored for these compounds. In the 2015, neonicotinoid insecticides have been incorporated in the watch list of substances for a European Union monitoring program (495/2015/ EU). A new method using automated solid phase extraction (SPE) with polymeric cartridges (OASIS HLB) followed by LC-MS/MS provided good separation of the most common neonicotindoid compounds. The method was developed for the determination of four neonicotinoid insecticides (nitenpyram, thiamethoxam, clothianidin, acetamiprid) in surface water with low limit of quantification (0.3-0.9 ng/L, nanograms per liter). Recoveries in surface water samples fortified at 200 ng/L for each compound ranged from 71.4 to 109.9 %; relative standard deviation ranged from 4 to 9%. The method was applied to water samples from four streams in Romania, Danube River and its tributaries (Arges River, Jiu River, and Olt River). The surface water samples were found to be contaminated clothianidin (1.08-6.4 ng/L) and by thiamethoxam (1.1-3.8ng/L). The highest concentrations were recorded in Danube River in Oltenita point (6.4ng/L) and in Gura-Vaii point (5.5ng/L). The concentration of acetamiprid and nitenpyram were situated below limit of quantification in all samples.

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