scholarly journals Prevalence of Bartonella clarridgeiae and Bartonella henselae in Domestic Cats from France and Detection of the Organisms in Erythrocytes by Immunofluorescence

2004 ◽  
Vol 11 (2) ◽  
pp. 423-425 ◽  
Author(s):  
Jean-Marc Rolain ◽  
Caroline Locatelli ◽  
Luc Chabanne ◽  
Bernard Davoust ◽  
Didier Raoult
Keyword(s):  
Bartonella Henselae ◽  
Domestic Cats ◽  
First Time ◽  
Number Of Bacteria

ABSTRACT The prevalence of Bartonella infection in a pet cat population from France was found to be 8.1% (8 of 99 cats). The intraerythrocytic location of Bartonella clarridgeiae is shown for the first time, and we show that immunofluorescence detection of the organism in erythrocytes correlates with the number of bacteria in blood.

scholarly journals Prevalence, hematological findings and genetic diversity ofBartonellaspp. in domestic cats from Valdivia, Southern Chile

Parasitology ◽  
2016 ◽  
Vol 144 (6) ◽  
pp. 773-782 ◽  
Author(s):  
ANANDA MÜLLER ◽  
ROMINA WALKER ◽  
PEDRO BITTENCOURT ◽  
ROSANGELA ZACARIAS MACHADO ◽  
JYAN LUCAS BENEVENUTE ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Nucleotide Diversity ◽  
Complete Blood Count ◽  
Bartonella Henselae ◽  
Hematological Parameters ◽  
Species Differentiation ◽  
Southern Chile ◽  
Domestic Cats ◽  
Real Time Quantitative Pcr ◽  
First Time

SUMMARYThe present study determined the prevalence, hematological findings and genetic diversity ofBartonellaspp. in domestic cats from Valdivia, Southern Chile. A complete blood count andnuoGgene real-time quantitative PCR (qPCR) forBartonellaspp. were performed in 370 blood samples from cats in Valdivia, Southern Chile.nuoGqPCR-positive samples were submitted to conventional PCR for thegltAgene and sequencing for species differentiation and phylogenetic analysis. Alignment ofgltAgene was used to calculate the nucleotide diversity, polymorphic level, number of variable sites and average number of nucleotide differences.BartonellaDNA prevalence in cats was 18·1% (67/370). Twenty-nine samples were sequenced with 62·0% (18/29) identified asBartonella henselae, 34·4% (10/29) asBartonella clarridgeiae, and 3·4% (1/29) asBartonella koehlerae. Bartonella-positive cats had low DNA bacterial loads and their hematological parameters varied minimally. EachBartonellaspecies from Chile clustered together and with otherBartonellaspp. described in cats worldwide.Bartonella henselaeandB. clarridgeiaeshowed a low number of variable sites, haplotypes and nucleotide diversity.Bartonella clarridgeiaeandB. koehleraeare reported for the first time in cats from Chile and South America, respectively.

scholarly journals Molecular Survey of Vector-Borne Pathogens of Dogs and Cats in Two Regions of Saudi Arabia

Pathogens ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 25
Author(s):  
Abdullah D. Alanazi ◽  
Abdulaziz S. Alouffi ◽  
Mohamed S. Alyousif ◽  
Mohammad Y. Alshahrani ◽  
Hend H. A. M. Abdullah ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Real Time ◽  
Real Time Pcr ◽  
Bartonella Henselae ◽  
Public Awareness ◽  
Effective Control ◽  
Vector Borne Diseases ◽  
Babesia Vogeli ◽  
Vector Borne ◽  
First Time

Dogs and cats play an important role as reservoirs of vector-borne pathogens, yet reports of canine and feline vector-borne diseases in Saudi Arabia are scarce. Blood samples were collected from 188 free-roaming dogs and cats in Asir (70 dogs and 44 cats) and Riyadh (74 dogs), Saudi Arabia. The presence of Anaplasma spp., Bartonella spp., hemotropic Mycoplasma spp., Babesia spp., and Hepatozoon spp. was detected using a multiplex tandem real-time PCR. PCR-positive samples were further examined with specific conventional and real-time PCR followed by sequencing. Dogs from Riyadh tested negative for all pathogens, while 46 out of 70 dogs (65.7%) and 17 out of 44 cats (38.6%) from Asir were positive for at least one pathogen. Positive dogs were infected with Anaplasma platys (57.1%), Babesia vogeli (30%), Mycoplasma haemocanis (15.7%), and Bartonella henselae (1.4%), and cats were infected with Mycoplasma haemofelis (13.6%), Candidatus Mycoplasma haemominutum (13.6%), B. henselae (9.2%), and A. platys (2.27%), all of which are reported for the first time in Saudi Arabia. Co-infection with A. platys and B. vogeli was detected in 17 dogs (24.28%), while coinfections were not detected in cats. These results suggest that effective control and public awareness strategies for minimizing infection in animals are necessary.

Infection of human CD34+ progenitor cells with Bartonella henselae results in intraerythrocytic presence of B henselae

Blood ◽  
2005 ◽  
Vol 106 (4) ◽  
pp. 1215-1222 ◽  
Author(s):  
Tanja Mändle ◽  
Hermann Einsele ◽  
Martin Schaller ◽  
Diana Neumann ◽  
Wichard Vogel ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Laser Scanning ◽  
Bartonella Henselae ◽  
Bacterial Pathogen ◽  
Erythroid Differentiation ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Facs Analysis ◽  
Human Cd34 ◽  
First Time

Abstract Although there is evidence that endothelial cells are important targets for human pathogenic Bartonella species, the primary niche of infection is unknown. Here we elucidated whether human CD34+ hematopoietic progenitor cells (HPCs) internalize B henselae and may serve as a potential niche of the pathogen. We showed that B henselae does not adhere to or invade human erythrocytes. In contrast, B henselae invades and persists in HPCs as shown by gentamicin protection assays, confocal laser scanning microscopy (CLSM), and electron microscopy (EM). Fluorescence-activated cell sorting (FACS) analysis of glycophorin A expression revealed that erythroid differentiation of HPCs was unaffected following infection with B henselae. The number of intracellular B henselae continuously increased over a 13-day period. When HPCs were infected with B henselae immediately after isolation, intracellular bacteria were subsequently detectable in differentiated erythroid cells on day 9 and day 13 after infection, as shown by CLSM, EM, and FACS analysis. Our data provide, for the first time, evidence that a bacterial pathogen is able to infect and persist in differentiating HPCs, and suggest that HPCs might serve as a potential primary niche in Bartonella infections.

scholarly journals Sero-prevalence of Bartonella henselae Infection in Domestic Cats and Dogs in Yamaguchi Prefecture, Japan

2004 ◽  
Vol 57 (4) ◽  
pp. 257-261
Author(s):  
Masaaki TOMITA ◽  
Junko YABATA ◽  
Kiyoshi TOMINAGA ◽  
Masatoshi SUGAWARA ◽  
Motohide HASHIMOTO ◽  
...  
Keyword(s):  
Bartonella Henselae ◽  
Domestic Cats

scholarly journals ASSESSMENT OF THE CURRENT STATE OF COASTAL WATERS OF THE SOUTHERN PART OF LAKE BAIKAL ON SANITARY AND MICROBIOLOGICAL INDICATORS WITH USE OF THE TEST SYSTEMS

2018 ◽  
pp. 54-58
Author(s):  
I.V. Mosharova ◽  
V.V. Il’inskij ◽  
O.V. Il’ina
Keyword(s):  
Lake Baikal ◽  
Coastal Waters ◽  
Test Systems ◽  
Current State ◽  
Wide Range ◽  
Microbial Number ◽  
Southern Site ◽  
First Time ◽  
Number Of Bacteria ◽  
Microbiological Indicators

Sanitary and microbiological researches of a coastal surface water of the southern part of Lake Baikal (from the Listvyanka to the Tanghui) were conducted in June, 2017. Total number of bacteria varied over a wide range – from 0.93 million сells/ml up to 2:05 million сells/ml, with an average 1.41 ± 0.3 million сells/ml. Biomass of bacteria varied from 11.05 to 305.00 mg С/m3, with an average of 123.34 mg С/m3. Coastal waters of the southern site of Lake Baikal mainly had a beta-meso-saprobic status in June, 2017. The total microbial number was determined with the use of the test systems for the first time. The values of the total microbial number were less than 1 000 COU/ml and varied from 9 to 412 COU/ml. Innovative test systems Petrifilm are of great interest for rapid assessments of the sanitary and microbiological status of reservoirs directly in the field conditions.

scholarly journals Bartonella henselae prevalence in domestic cats in California: risk factors and association between bacteremia and antibody titers.

1995 ◽  
Vol 33 (9) ◽  
pp. 2445-2450 ◽  
Author(s):  
B B Chomel ◽  
R C Abbott ◽  
R W Kasten ◽  
K A Floyd-Hawkins ◽  
P H Kass ◽  
...  
Keyword(s):  
Risk Factors ◽  
Bartonella Henselae ◽  
Domestic Cats ◽  
Antibody Titers

scholarly journals Survey of lungworm infection of domestic cats in Hungary

2019 ◽  
Vol 67 (3) ◽  
pp. 407-417 ◽  
Author(s):  
Sára Kiszely ◽  
Mónika Gyurkovszky ◽  
Norbert Solymosi ◽  
Róbert Farkas
Keyword(s):  
Clinical Signs ◽  
Morphological Identification ◽  
Domestic Cats ◽  
Aelurostrongylus Abstrusus ◽  
Respiratory Disorders ◽  
First Instar ◽  
Faecal Samples ◽  
First Time ◽  
Apparently Healthy

From 61 settlements of 12 Hungarian counties, 303 domestic cats were included in this survey. Between autumn 2016 and spring 2018, fresh faecal samples were randomly collected and examined by flotation and by the Baermann–Wetzel method for the presence of lungworm infection. No eggs of Eucoleus aerophilus were detected. Morphological identification of first instar larvae (L1) was also carried out. In the faeces of 60 cats (19.8%) from 17 settlements and Budapest, L1 of Aelurostrongylus abstrusus were found. More than half of the cats were from the western part of the country. The average number of larvae per gram of faeces was 190.2 ± 304.88. These results are in line with the former findings on the prevalence of aelurostrongylosis of domestic cats in Hungary. In addition, Oslerus rostratus was also found for the first time in the faecal samples of three cats from the eastern part of the country, infected also with Ae. abstrusus. The average age (2.51 ± 1.26 years) of infected cats indicates that lungworm infection is more common among younger cats. No relationship was found between the lung-worm infection and the sex of cats. Non-neutered cats had a significantly higher proportion of lungworm infections. Two-thirds of the infected cats were apparently healthy, and only 19 individuals showed clinical signs of respiratory disorders.

Occurrence of Bartonella henselae types I and II in Central Italian domestic cats

2012 ◽  
Vol 93 (1) ◽  
pp. 63-66 ◽  
Author(s):  
Valentina V. Ebani ◽  
Fabrizio Bertelloni ◽  
Filippo Fratini
Keyword(s):  
Bartonella Henselae ◽  
Domestic Cats

scholarly journals PCR detection of Bartonella spp. in the dog

2014 ◽  
Vol 83 (2) ◽  
pp. 79-82 ◽  
Author(s):  
Jarmila Konvalinová ◽  
Vlasta Svobodová ◽  
Dobromila Molinková ◽  
Miroslav Svoboda
Keyword(s):  
Polymerase Chain Reaction ◽  
Czech Republic ◽  
Clinical Symptoms ◽  
Bartonella Henselae ◽  
Chain Reaction ◽  
The Czech Republic ◽  
Two Samples ◽  
Healthy Dogs ◽  
Polymerase Chain ◽  
First Time

Our study aimed at using PCR to identify the incidence ofBartonellaspp. in blood of dogs. Altogether 286 dogs of 92 breeds aged 3 month to 17 years were tested from October 2008 to December 2009. Healthy dogs as well as dogs with various clinical symptoms of disease were included in the group. Samples were tested by polymerase chain reaction (PCR) specific for the presence ofBartonellaspp. Following the DNA examination in 286 dogs by PCR and subsequent sequencing, two samples were identified asBartonella henselae(0.7%). Other species ofBartonellawere not found. It was the first time in the Czech Republic when incidence ofBartonellaspp. was determined in dogs.

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