Whole-Genome Sequences of Seven Strains of
                    Bacillus cereus
                    Isolated from Foodstuff or Poisoning Incidents: TABLE 1

Abstract Background The complete genome sequences of 44 Bacillus cereus group isolates collected from diverse sources in Japan were analyzed to determine their genetic backgrounds and diversity levels in Japan. Multilocus sequence typing (MLST) and core-genome single-nucleotide polymorphism (SNP) typing data from whole-genome sequences were analyzed to determine genetic diversity levels. Virulence-associated gene profiles were also used to evaluate the genetic backgrounds and relationships among the isolates. Results The 44 B. cereus group isolates, including soil- and animal-derived isolates and isolates recovered from hospitalized patients and food poisoning cases, were genotyped by MLST and core-genome SNP typing. Genetic variation among the isolates was identified by the MLST and core-genome SNP phylogeny comparison against reference strains from countries outside of Japan. Exploratory principal component analysis and nonmetric multidimensional scaling (NMDS) analyses were used to assess the genetic similarities among the isolates using gene presence and absence information and isolate origins as the metadata. A significant correlation was seen between the principal components and the presence of genes encoding hemolysin BL and emetic genetic determinants in B. cereus, and the capsule proteins in B. anthracis. NMDS showed that the cluster of soil isolates overlapped with the cluster comprising animal-derived and clinical isolates. Conclusions Molecular and epidemiological analyses of B. cereus group isolates in Japan suggest that the soil- and animal-derived bacteria from our study are not a significant risk to human health. However, because several of the clinical isolates share close genetic relationships with the environmental isolates, both molecular and epidemiological surveillance studies could be used effectively to estimate virulence in these important pathogens.

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Draft Whole-Genome Sequences of 11 Bacillus cereus Food Isolates: TABLE 1

Genome Announcements ◽

10.1128/genomea.00485-16 ◽

2016 ◽

Vol 4 (3) ◽

Cited By ~ 8

Author(s):

Hasmik Hayrapetyan ◽

Jos Boekhorst ◽

Anne de Jong ◽

Oscar P. Kuipers ◽

Masja N. Nierop Groot ◽

...

Keyword(s):

Bacillus Cereus ◽

Foodborne Pathogen ◽

Whole Genome ◽

Genome Sequences

Bacillus cereus is a foodborne pathogen causing emetic and diarrheal-type syndromes. Here, we report the whole-genome sequences of 11 B. cereus food isolates.

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Whole-Genome Sequences of 94 Environmental Isolates of Bacillus cereus Sensu Lato

Genome Announcements ◽

10.1128/genomea.00380-13 ◽

2013 ◽

Vol 1 (5) ◽

Cited By ~ 16

Author(s):

G. A. Van der Auwera ◽

M. Feldgarden ◽

R. Kolter ◽

J. Mahillon

Keyword(s):

Bacillus Cereus ◽

Whole Genome ◽

Environmental Isolates ◽

Genome Sequences ◽

Bacillus Cereus Sensu Lato

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Next-Generation Whole-Genome Sequencing of Eight Strains of Bacillus cereus , Isolated from Food: TABLE 1

Genome Announcements ◽

10.1128/genomea.01480-15 ◽

2015 ◽

Vol 3 (6) ◽

Cited By ~ 6

Author(s):

Antonina O. Krawczyk ◽

Anne de Jong ◽

Robyn T. Eijlander ◽

Erwin M. Berendsen ◽

Siger Holsappel ◽

...

Keyword(s):

Bacillus Cereus ◽

Whole Genome Sequencing ◽

Genome Sequencing ◽

Foodborne Illness ◽

Food Sources ◽

Whole Genome ◽

Next Generation ◽

Genome Sequences

Bacillus cereus can contaminate food and cause emetic and diarrheal foodborne illness. Here, we report whole-genome sequences of eight strains of B. cereus , isolated from different food sources.

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Fulminant Bacillus cereus food poisoning with fatal multi-organ failure

BMJ Case Reports ◽

10.1136/bcr-2020-238716 ◽

2021 ◽

Vol 14 (1) ◽

pp. e238716

Author(s):

Clinton M G Colaco ◽

Kerri Basile ◽

Jenny Draper ◽

Patricia E Ferguson

Keyword(s):

Intensive Care ◽

Metabolic Acidosis ◽

Renal Replacement Therapy ◽

Bacillus Cereus ◽

Genome Sequencing ◽

Food Poisoning ◽

Food Preparation ◽

Whole Genome ◽

Blood Products ◽

And Storage

This case represents a rare fulminant course of fried-rice associated food poisoning in an immunocompetent person due to pre-formed exotoxin produced by Bacillus cereus, with severe manifestations of sepsis, including multi-organ (hepatic, renal, cardiac, respiratory and neurological) failure, shock, metabolic acidosis, rhabdomyolysis and coagulopathy. Despite maximal supportive measures (continuous renal replacement therapy, plasmapheresis, N-acetylcysteine infusion and blood products, and broad-spectrum antimicrobials) and input from a multidisciplinary team (consisting of infectious diseases, intensive care, gastroenterology, surgery, toxicology, immunology and haematology), mortality resulted. This case is the first to use whole genome sequencing techniques to confirm the toxigenic potential of B. cereus. It has important implications for food preparation and storage, particularly given its occurrence in home isolation during the COVID-19 pandemic.

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KEJADIAN LUAR BIASA KERACUNAN “CUMI-CUMIAN” DI SEKOLAH DASAR NEGERI 1 TRASAN BANDONGAN KABUPATEN MAGELANG

Media Ilmu Kesehatan ◽

10.30989/mik.v7i2.280 ◽

2018 ◽

Vol 7 (2) ◽

pp. 131-136

Author(s):

Nasir Ahmad

Keyword(s):

Abdominal Pain ◽

Bacillus Cereus ◽

Rhodamine B ◽

Attack Rate ◽

Food Poisoning ◽

Health Department ◽

Analysis Data ◽

Food Samples ◽

Bivariate Analysis ◽

School Committee

Background: On May 4th, 2016, at 12:30 district surveillance officer of Magelang Health Department received reports from Public Health Center of Bandongan about 21 students of SDN 1 Trasan who suffered from the same food-poisoning symptoms. Objective: Investigation was carried out to identify the source, how it spread and how to control it. Methods: This study used descriptive analytic and mapping the cases distribution location. The case was people experiencing symptoms of dizziness or abdominal pain or nausea or vomiting. Data analysis was done by using bivariate analysis. Data collection were done through interviews, observations and laboratory tests on the food samples. Results: The case was 50 students (from 1-6 grade students). The perceived symptoms were dizziness (77%), nausea (42%), abdominal pain (40%) and vomiting (8%). Attack rate found ranged from 14.3% to 60% with the highest Attack rate found on class three (60%). The incubation period of 15-240 minutes (mean 72.3 minutes). Calamari like positive Bacillus cereus and Rhodamine-B 10 mg/kg. Conclusion: The outbreak of food poisoning because calamari like contaminated Bacillus cereus. We suggested the school committee to provide the socialization of harmful food for the students. The teachers should restrict the permission for the food vendor to sell at school. Keywords: Bacillus cereus, , Food Poisoning, Outbreak, Rhodamine B, School Food

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