scholarly journals Draft Genomic Sequencing of Six Potential Extraintestinal Pathogenic Escherichia coli Isolates from Retail Chicken Meat

2018 ◽  
Vol 6 (21) ◽  
Author(s):  
Aixia Xu ◽  
James R. Johnson ◽  
Shiowshuh Sheen ◽  
David S. Needleman ◽  
Christopher Sommers

ABSTRACT Potential extraintestinal pathogenic Escherichia coli strains DP254, WH333, WH398, F356, FEX675, and FEX725 were isolated from retail chicken meat products. Here, we report the draft genome sequences for these six E. coli isolates, which are currently being used in food safety research.

2014 ◽  
Vol 81 (3) ◽  
pp. 1177-1187 ◽  
Author(s):  
Natalie M. Mitchell ◽  
James R. Johnson ◽  
Brian Johnston ◽  
Roy Curtiss ◽  
Melha Mellata

ABSTRACTChicken products are suspected as a source of extraintestinal pathogenicEscherichia coli(ExPEC), which causes diseases in humans. The zoonotic risk to humans from chicken-sourceE. coliis not fully elucidated. To clarify the zoonotic risk posed by ExPEC in chicken products and to fill existing knowledge gaps regarding ExPEC zoonosis, we evaluated the prevalence of ExPEC on shell eggs and compared virulence-associated phenotypes between ExPEC and non-ExPEC isolates from both chicken meat and eggs. The prevalence of ExPEC among egg-source isolates was low, i.e., 5/108 (4.7%). Based on combined genotypic and phenotypic screening results, multiple human and avian pathotypes were represented among the chicken-source ExPEC isolates, including avian-pathogenicE. coli(APEC), uropathogenicE. coli(UPEC), neonatal meningitisE. coli(NMEC), and sepsis-associatedE. coli(SEPEC), as well as an undefined ExPEC group, which included isolates with fewer virulence factors than the APEC, UPEC, and NMEC isolates. These findings document a substantial prevalence of human-pathogenic ExPEC-associated genes and phenotypes amongE. coliisolates from retail chicken products and identify key virulence traits that could be used for screening.


2017 ◽  
Vol 5 (20) ◽  
Author(s):  
Daniel F. Monte ◽  
Miriam R. Fernandes ◽  
Louise Cerdeira ◽  
Tiago A. de Souza ◽  
Andressa Mem ◽  
...  

ABSTRACT We present here the draft genome sequences of two colistin-resistant mcr-1-carrying Escherichia coli strains belonging to sequence type 74 (ST74) and ST1850, isolated from commercial chicken meat in Brazil. Assembly of this draft genome resulted in 5,022,083 and 4,950,681 bp, respectively, revealing the presence of the IncX4 plasmid-mediated mcr-1 gene responsible for resistance to colistin.


2018 ◽  
Vol 7 (7) ◽  
Author(s):  
Aixia Xu ◽  
Shannon Tilman ◽  
Kristy Wisser-Parker ◽  
O. Joseph Scullen ◽  
Christopher H. Sommers

Extraintestinal pathogenic Escherichia coli strains were isolated from retail chicken skin. Here, we report the draft genomic sequences for these nine E. coli isolates, which are currently being used in agricultural and food safety research.


2017 ◽  
Vol 5 (11) ◽  
Author(s):  
Soo Jin Jeon ◽  
Federico Cunha ◽  
Amber Ginn ◽  
KwangCheol Casey Jeong ◽  
Klibs N. Galvão

ABSTRACT Escherichia coli is involved in the pathogenicity of metritis in cows. We report here the genome sequences of E. coli strains isolated at calving from the uterus, vagina, vulva, and rectoanal junction of a dairy cow that later developed metritis. The genomic similarities will give an insight into phylogenetic relationships among strains.


2021 ◽  
Vol 10 (23) ◽  
Author(s):  
Michael J. Sikorski ◽  
Tracy H. Hazen ◽  
Gopi Vyas ◽  
Jane M. Michalski ◽  
David A. Rasko

There are six described pathotypes of Escherichia coli that cause significant clinical illness in humans. Enteroinvasive E. coli (EIEC) strains have been shown to be separated into three phylogenomic clades. To add to a limited body of EIEC genomic data, we report two high-quality draft genomes representing different EIEC phylogenomic clades.


2021 ◽  
Vol 10 (7) ◽  
Author(s):  
Dongyun Jung ◽  
Soyoun Park ◽  
Janina Ruffini ◽  
Simon Dufour ◽  
Jennifer Ronholm

ABSTRACT Escherichia coli is one of the most common etiological agents responsible for clinical bovine mastitis. Here, we report the draft genome sequences and annotations of 113 E. coli strains that were isolated from Holstein cows with intramammary infections in Canada.


2018 ◽  
Vol 6 (11) ◽  
Author(s):  
Aixia Xu ◽  
Sarah Hertrich ◽  
David S. Needleman ◽  
Shiowshuh Sheen ◽  
Christopher Sommers

ABSTRACT Uropathogenic Escherichia coli serotype O4:H5 isolates (ATCC 700414, 700415, 700416, and 700417) were recovered from women with first-time urinary tract infections. Here, we report the draft genome sequences for these four E. coli isolates, which are currently being used to validate food safety processing technologies.


2017 ◽  
Vol 5 (18) ◽  
Author(s):  
Muhammad A. Rehman ◽  
Catherine Carrillo ◽  
François Malouin ◽  
Moussa S. Diarra

ABSTRACT Enterohemorrhagic Escherichia coli serotype O157:H7 is a major cause of foodborne outbreaks and hemolytic-uremic syndrome. Here, we report the draft genome sequences of three antibiotic-resistant E. coli O157:H7 strains isolated from feedlot cattle. These draft genome sequences will aid in the development of sequence-based tools for the detection of virulence and antimicrobial resistance genotypes.


2018 ◽  
Vol 6 (16) ◽  
pp. e00091-18 ◽  
Author(s):  
Aixia Xu ◽  
James R. Johnson ◽  
Shiowshuh Sheen ◽  
Christopher Sommers

ABSTRACTNeonatal meningitis-causingEscherichia coliisolates (SP-4, SP-5, SP-13, SP-46, and SP-65) were recovered between 1989 and 1997 from infants in the Netherlands. Here, we report the draft genome sequences of these fiveE. coliisolates, which are currently being used to validate food safety processing technologies.


2017 ◽  
Vol 5 (4) ◽  
Author(s):  
Annika Cimdins ◽  
Petra Lüthje ◽  
Fengyang Li ◽  
Irfan Ahmad ◽  
Annelie Brauner ◽  
...  

ABSTRACT Strains of Escherichia coli exhibit diverse biofilm formation capabilities. E. coli K-12 expresses the red, dry, and rough (rdar) morphotype below 30°C, whereas clinical isolates frequently display the rdar morphotype semiconstitutively. We sequenced the genomes of eight E. coli strains to subsequently investigate the molecular basis of semiconstitutive rdar morphotype expression.


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