Genome-Wide Identification of H-NS-Controlled, Temperature-Regulated Genes in Escherichiacoli K-12

ABSTRACT DNA microarrays demonstrate that H-NS controls 69% of the temperature regulated genes in Escherichia coli K-12. H-NS is shown to be a common regulator of multiple iron and other nutrient acquisition systems preferentially expressed at 37°C and of general stress response, biofilm formation, and cold shock genes highly expressed at 23°C.

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THE ROLE OF GENERAL STRESS RESPONSE REGULATOR RpoS IN BIOFILM FORMATION BY ESCHERICHIA COLI IN THE PRESENCE OF PLANT POLYPHENOLS

Book of proceedings of the All-Russian Scientific Conference with International Participation and Schools of Young Scientists "Mechanisms of resistance of plants and microorganisms to unfavorable environmental" (parts I, II) ◽

10.31255/978-5-94797-319-8-703-705 ◽

2018 ◽

Author(s):

Z.Y. Samoylova ◽

G.V. Smirnova ◽

O.N. Oktyabrsky

Keyword(s):

Escherichia Coli ◽

Stress Response ◽

Biofilm Formation ◽

Response Regulator ◽

Plant Polyphenols ◽

General Stress Response ◽

General Stress

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Genome-wide analysis of the general stress response in Bacillus subtilis

Molecular Microbiology ◽

10.1046/j.1365-2958.2001.02534.x ◽

2002 ◽

Vol 41 (4) ◽

pp. 757-774 ◽

Cited By ~ 195

Author(s):

Chester W. Price ◽

Paul Fawcett ◽

Hélène Cérémonie ◽

Nancy Su ◽

Christopher K. Murphy ◽

...

Keyword(s):

Bacillus Subtilis ◽

Stress Response ◽

General Stress Response ◽

Genome Wide Analysis ◽

General Stress ◽

Genome Wide

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Transcriptome analysis of intraspecific competition in Arabidopsis thaliana reveals organ-specific signatures related to nutrient acquisition and general stress response pathways

BMC Plant Biology ◽

10.1186/1471-2229-12-227 ◽

2012 ◽

Vol 12 (1) ◽

pp. 227 ◽

Cited By ~ 18

Author(s):

Frédéric G Masclaux ◽

Friederike Bruessow ◽

Fabian Schweizer ◽

Caroline Gouhier-Darimont ◽

Laurent Keller ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Stress Response ◽

Transcriptome Analysis ◽

Intraspecific Competition ◽

Nutrient Acquisition ◽

General Stress Response ◽

General Stress ◽

Organ Specific

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Genome-Wide Analysis of the General Stress Response Network in Escherichia coli: σS-Dependent Genes, Promoters, and Sigma Factor Selectivity

Journal of Bacteriology ◽

10.1128/jb.187.5.1591-1603.2005 ◽

2005 ◽

Vol 187 (5) ◽

pp. 1591-1603 ◽

Cited By ~ 514

Author(s):

Harald Weber ◽

Tino Polen ◽

Johanna Heuveling ◽

Volker F. Wendisch ◽

Regine Hengge

Keyword(s):

Escherichia Coli ◽

Stress Response ◽

Rna Polymerase ◽

Sigma Factor ◽

Regulatory Network ◽

Cell Physiology ◽

Data Set ◽

General Stress Response ◽

General Stress ◽

Genome Wide

ABSTRACT The σS (or RpoS) subunit of RNA polymerase is the master regulator of the general stress response in Escherichia coli. While nearly absent in rapidly growing cells, σS is strongly induced during entry into stationary phase and/or many other stress conditions and is essential for the expression of multiple stress resistances. Genome-wide expression profiling data presented here indicate that up to 10% of the E. coli genes are under direct or indirect control of σS and that σS should be considered a second vegetative sigma factor with a major impact not only on stress tolerance but on the entire cell physiology under nonoptimal growth conditions. This large data set allowed us to unequivocally identify a σS consensus promoter in silico. Moreover, our results suggest that σS-dependent genes represent a regulatory network with complex internal control (as exemplified by the acid resistance genes). This network also exhibits extensive regulatory overlaps with other global regulons (e.g., the cyclic AMP receptor protein regulon). In addition, the global regulatory protein Lrp was found to affect σS and/or σ70 selectivity of many promoters. These observations indicate that certain modules of the σS-dependent general stress response can be temporarily recruited by stress-specific regulons, which are controlled by other stress-responsive regulators that act together with σ70 RNA polymerase. Thus, not only the expression of genes within a regulatory network but also the architecture of the network itself can be subject to regulation.

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ygsIs a Novel Gene That Influences Biofilm Formation and the General Stress Response ofStaphylococcus epidermidis

Infection and Immunity ◽

10.1128/iai.00916-10 ◽

2010 ◽

Vol 79 (3) ◽

pp. 1007-1015 ◽

Cited By ~ 13

Author(s):

Xing Wang ◽

Chen Niu ◽

Gang Sun ◽

Dandan Dong ◽

Amer E. Villaruz ◽

...

Keyword(s):

Stress Response ◽

Staphylococcus Epidermidis ◽

Biofilm Formation ◽

Gene Encoding ◽

General Stress Response ◽

General Stress ◽

Infection Models ◽

Implanted Medical Devices ◽

Biofilm Development ◽

Stress Response Gene

ABSTRACTInfections caused by the nosocomial pathogenStaphylococcus epidermidisfrequently develop on implanted medical devices and involve biofilm formation. Biofilms are surface-attached microbial communities that show increased resistance to drug treatment and mechanisms of innate host defense. In this study, a mutant library of the clinical isolateS. epidermidis1457 was constructed using mariner-based transposon mutagenesis. About a thousand mutants were screened, and 12 mutants were identified as significantly defective in biofilm formation. We focused on a mutant in which the transposon had inserted in a gene with unknown function,SERP0541, which is annotated as a gene encoding a GSP13-like general stress response protein. The gene was namedygs(encoding an unknowngeneralstress protein). Various stresses, including heat, pH, high osmolarity, and ethanol affected the survival of theygsmutant to a significantly higher degree than the wild-type strain and led to increased expression ofygs. Furthermore, synthesis of polysaccharide intercellular adhesin (PIA) and transcription of the PIA biosynthetic operon were significantly decreased in theygsmutant. These results are in accordance with the putative involvement ofygsin stress-response gene regulation and indicate thatygsinfluences biofilm development by controlling PIA-dependent biofilm accumulation. Moreover,ygshad a significant impact on the formation of biofilms and metastatic disease in two catheter-related rat infection models. Our study shows that theygsgene controlsS. epidermidisbiofilm accumulation and stress resistance, representing a key regulator of both structural and physiological biofilm characteristics with a significant impact on biofilm-associated infection.

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