scholarly journals Physical and Genetic Map of the Obligate Intracellular Bacterium Coxiella burnetii

1998 ◽  
Vol 180 (15) ◽  
pp. 3816-3822 ◽  
Author(s):  
H. Willems ◽  
Cornelie Jäger ◽  
Georg Baljer
Keyword(s):  
Genetic Map ◽  
Coxiella Burnetii ◽  
Physical Map ◽  
Gene Organization ◽  
Intracellular Bacterium ◽  
Rrna Gene ◽  
Obligate Intracellular Bacterium ◽  
Obligate Intracellular ◽  
Great Heterogeneity ◽  
Physical And Genetic Map

ABSTRACT Pulsed-field gel electrophoresis and PCR techniques have been used to construct a NotI macrorestriction map of the obligate intracellular bacterium Coxiella burnetii Nine Mile. The size of the chromosome has been determined to be 2,103 kb comprising 29NotI restriction fragments. The average resolution is 72.5 kb, or about 3.5% of the genome. Experimental data support the presence of a linear chromosome. Published genes were localized on the physical map by Southern hybridization. One gene, recognized as transposable element, was found to be present in at least nine sites evenly distributed over the whole chromosome. There is only one copy of a 16S rRNA gene. The putative oriC has been located on a 27.5-kb NotI fragment. Gene organization upstream theoriC is almost identical to that of Pseudomonas putida and Bacillus subtilis, whereas gene organization downstream the oriC seems to be unique among bacteria. The physical map will be helpful in investigations of the great heterogeneity in restriction fragment length polymorphism patterns of different isolates and the great variation in genome size. The genetic map will help to determine whether gene order in different isolates is conserved.

scholarly journals Molecular pathogenesis of the obligate intracellular bacterium Coxiella burnetii

2013 ◽  
Vol 11 (8) ◽  
pp. 561-573 ◽  
Author(s):  
Erin J. van Schaik ◽  
Chen Chen ◽  
Katja Mertens ◽  
Mary M. Weber ◽  
James E. Samuel
Keyword(s):  
Coxiella Burnetii ◽  
Molecular Pathogenesis ◽  
Intracellular Bacterium ◽  
Obligate Intracellular Bacterium ◽  
Obligate Intracellular

Effects of temperature upon the cell-free translation system from Coxiella burnetii

1987 ◽  
Vol 33 (7) ◽  
pp. 642-646 ◽  
Author(s):  
John P. Donahue ◽  
Herbert A. Thompson
Keyword(s):  
Protein Synthesis ◽  
Coxiella Burnetii ◽  
Intracellular Bacterium ◽  
Physiological Characteristics ◽  
Translation System ◽  
Obligate Intracellular Bacterium ◽  
Rna Translation ◽  
Obligate Intracellular ◽  
Free Translation ◽  
Effects Of Temperature

The rate and extent of coliphage Qβ RNA translation by cell-free extracts prepared from Coxiella burnetii were studied. When translations were conducted at temperatures elevated above 37 °C, both polypeptide elongation and frequency of initiation were by comparison increased. The ratios of products synthesized from the polycistronic phage mRNA also changed upon increases in translation temperature, especially at 45 °C. Although the organism is a moderate acidophile, initiation of protein synthesis in extracts did not occur below pH 6.2, and was superior when the pH was 6.8–7.2. The results are discussed in context with the known physiological characteristics of this obligate intracellular bacterium.

scholarly journals Efficient Method of Cloning the Obligate Intracellular Bacterium Coxiella burnetii

2007 ◽  
Vol 73 (12) ◽  
pp. 4048-4054 ◽  
Author(s):  
Paul A. Beare ◽  
Dale Howe ◽  
Diane C. Cockrell ◽  
Robert A. Heinzen
Keyword(s):  
Coxiella Burnetii ◽  
Parasitophorous Vacuole ◽  
Vero Cells ◽  
Intracellular Bacterium ◽  
Obligate Intracellular Bacterium ◽  
Screening Process ◽  
Pcr Screening ◽  
Cell Monolayers ◽  
Obligate Intracellular ◽  
Cloning Method

ABSTRACT Coxiella burnetii is an obligate intracellular bacterium that replicates in a large lysosome-like parasitophorous vacuole (PV). Current methods of cloning C. burnetii are laborious and technically demanding. We have developed an alternative cloning method that involves excision of individual C. burnetii-laden PVs from infected cell monolayers by micromanipulation. To demonstrate the cloning utility and efficiency of this procedure, we coinfected Vero cells with isogenic variants of the Nine Mile strain of C. burnetii. Coinhabited PVs harboring Nine Mile phase II (NMII) and Nine Mile phase I (NMI) or Nine Mile crazy (NMC) were demonstrated by immunofluorescence. PVs were then randomly excised from cells coinfected with NMI and NMC by micromanipulation, and PVs harboring both strains were identified by PCR. Fresh Vero cells were subsequently infected with organisms from coinhabited PVs, and the PV excision and PCR screening process was repeated. Without exception, PVs obtained from second-round excisions contained clonal populations of either NMII or NMC, demonstrating that micromanipulation is an efficient and reproducible procedure for obtaining C. burnetii clones.

Host range of obligate intracellular bacterium Parachlamydia acanthamoebae

2010 ◽  
Vol 54 (11) ◽  
pp. 707-713 ◽  
Author(s):  
Yasuhiro Hayashi ◽  
Shinji Nakamura ◽  
Junji Matsuo ◽  
Tatsuya Fukumoto ◽  
Mitsutaka Yoshida ◽  
...  
Keyword(s):  
Host Range ◽  
Intracellular Bacterium ◽  
Obligate Intracellular Bacterium ◽  
Obligate Intracellular

scholarly journals Discovery of Putative Small Non-Coding RNAs from the Obligate Intracellular Bacterium Wolbachia pipientis

PLoS ONE ◽  
2015 ◽  
Vol 10 (3) ◽  
pp. e0118595 ◽  
Author(s):  
Megan Woolfit ◽  
Manjula Algama ◽  
Jonathan M. Keith ◽  
Elizabeth A. McGraw ◽  
Jean Popovici
Keyword(s):  
Intracellular Bacterium ◽  
Obligate Intracellular Bacterium ◽  
Wolbachia Pipientis ◽  
Obligate Intracellular ◽  
Non Coding Rnas

scholarly journals Antibody-Mediated Elimination of the Obligate Intracellular Bacterial Pathogen Ehrlichia chaffeensisduring Active Infection

2000 ◽  
Vol 68 (4) ◽  
pp. 2187-2195 ◽  
Author(s):  
Gary M. Winslow ◽  
Eric Yager ◽  
Konstantin Shilo ◽  
Erin Volk ◽  
Andrew Reilly ◽  
...  
Keyword(s):  
Bacterial Pathogen ◽  
Immune Serum ◽  
Intracellular Bacterium ◽  
Bacterial Clearance ◽  
Obligate Intracellular Bacterium ◽  
Active Infection ◽  
Obligate Intracellular ◽  
Humoral Responses ◽  
Human Monocytic Ehrlichiosis ◽  
Dose Dependent

ABSTRACT It is generally accepted that cellular, but not humoral immunity, plays an important role in host defense against intracellular bacteria. However, studies of some of these pathogens have provided evidence that antibodies can provide immunity if present during the initiation of infection. Here, we examined immunity against infection byEhrlichia chaffeensis, an obligate intracellular bacterium that causes human monocytic ehrlichiosis. Studies with mice have demonstrated that immunocompetent strains are resistant to persistent infection but that SCID mice become persistently and fatally infected. Transfer of immune serum or antibodies obtained from immunocompetent C57BL/6 mice to C57BL/6 scid mice provided significant although transient protection from infection. Bacterial clearance was observed when administration occurred at the time of inoculation or well after infection was established. The effect was dose dependent, occurred within 2 days, and persisted for as long as 2 weeks. Weekly serum administration prolonged the survival of susceptible mice. Although cellular immunity is required for complete bacterial clearance, the data show that antibodies can play a significant role in the elimination of this obligate intracellular bacterium during active infection and thus challenge the paradigm that humoral responses are unimportant for immunity to such organisms.

A study of the correlation between Coxiella burnetii seropositivity and abortions in sheep in Eastern and Southeastern Turkey

2016 ◽  
Author(s):  
Ayse Kilic ◽  
Hakan Kalender
Keyword(s):  
Immunosorbent Assay ◽  
Coxiella Burnetii ◽  
Q Fever ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Eastern Anatolia ◽  
Obligate Intracellular Bacterium ◽  
Serum Samples ◽  
Obligate Intracellular ◽  
Elisa Kit

Q fever is a zoonotic disease that occurs worldwide and is caused by the obligate intracellular bacterium Coxiella burnetii. Infected animals are usually asymptomatic, but infection can cause abortion and stillbirth in ruminants. The main purpose of this study was to evaluate prevalance of Coxiella burnetii infection in aborted and nonaborted sheep serum samples in Eastern Anatolia region by using enzyme-linked immunosorbent assay (ELISA). The determine of prevalance in sheep flocks from four provinces (Elazig, Malatya, Tunceli, Bitlis) and tested for anti-C.burnetii antibody detection, by means of Chekit Q fever Elisa kit. 350 serum samples obtained from flocks belonging aborted sheep showed that a total of 56 (16%) were detected seropositivity, whereas 171 serum samples obtained from nonaborted sheep flocks in 13 of the 171 (7.60%) for C.burnetii in seropositivity were observed. Coxiellosis should be considered an important cause of sheep with abortion history and nonaborted in Elazig and neighboring provinces.

scholarly journals Whole-Genome Enrichment and Sequencing of Chlamydia trachomatis Directly from Patient Clinical Vaginal and Rectal Swabs

2020 ◽  
Author(s):  
Katherine E. Bowden ◽  
Sandeep J. Joseph ◽  
John Cartee ◽  
Noa Ziklo ◽  
Damien Danavall ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Intracellular Bacterium ◽  
Clinical Samples ◽  
Sexual Networks ◽  
Whole Genome ◽  
Target Enrichment ◽  
Obligate Intracellular Bacterium ◽  
Obligate Intracellular ◽  
Improve Efficiency ◽  
Sex With Men

AbstractChlamydia trachomatis is the most prevalent cause of bacterial sexually transmitted infections (STIs) worldwide. U.S. cases have been steadily increasing for more than a decade in both the urogenital tract and rectum. C. trachomatis is an obligate intracellular bacterium that is not easily cultured, limiting the capacity for genome studies to understand strain diversity and emergence among various patient populations globally. While Agilent SureSelectXT target-enrichment RNA bait libraries have been developed for whole-genome enrichment and sequencing of C. trachomatis directly from clinical urine, vaginal, conjunctival and rectal samples, efficiencies are only 60-80% for ≥95-100% genome coverage. We therefore re-designed and expanded the RNA bait library to augment enrichment of the organism from clinical samples to improve efficiency. We describe the expanded library, the limit of detection for C. trachomatis genome copy input, and the 100% efficiency and high-resolution of generated genomes where genomic recombination among paired vaginal and rectal specimens from four patients was identified. This workflow provides a robust approach for discerning genomic diversity and advancing our understanding of the molecular epidemiology of contemporary C. trachomatis STIs across sample types, among geographic populations, sexual networks, and outbreaks associated with proctitis/proctocolitis among women and men who have sex with men.ImportanceChlamydia trachomatis is an obligate intracellular bacterium that is not easily cultured, and there is limited information on rectal C. trachomatis transmission and its impact on morbidity. To improve efficiency of previous studies involving whole genome target enrichment and sequencing of C. trachomatis directly from clinical urine, vaginal, conjunctival, and rectal specimens, we expanded the RNA bait library to augment enrichment of the organism from clinical samples. We demonstrate an increased efficiency in the percentage of reads mapping to C. trachomatis. We show the new system is sensitive for near identical genomes of C. trachomatis from two body sites in four women. Further, we provide a robust genomic epidemiologic approach to advance our understanding of C. trachomatis strains causing ocular, urogenital and rectal infections, and to explore geo-sexual networks, outbreaks of colorectal infections among women and men who have sex with men, and the role of these strains in morbidity.

Sign in / Sign up

Export Citation Format

Share Document