scholarly journals Molecular Diagnostics of Hemorrhagic Fever with Renal Syndrome during a Dobrava Virus Infection Outbreak in the European Part of Russia

2009 ◽  
Vol 47 (12) ◽  
pp. 4029-4036 ◽  
Author(s):  
T. K. Dzagurova ◽  
B. Klempa ◽  
E. A. Tkachenko ◽  
G. P. Slyusareva ◽  
V. G. Morozov ◽  
...  
Author(s):  
A. S. Volynkina ◽  
A. N. Kulichenko

Presented are the results of gene-typing of CCHF virus detected in clinical samples from CHF patients from the Stavropol, Rostov and Astrakhan Regions in 2011. For 28 samples determined are nucleotide sequences of the fragments 115–652 (S segment) and fragments 984–1469 (M segment). Philogenetic analysis of these nucleotide sequences demonstrated that typical strains circulated in 2011 in the regions under surveillance, importation of the new genetic variants of the virus did not take place. CCHF virus variant affiliated to the subgroup “Astrakhan-2” was detected in the clinical samples for the first time and characterized for its genome S- and M-segment fragments.


Author(s):  
Vladimir Ternovoi ◽  
Anastasia Gladysheva ◽  
Alexandra Sementsova ◽  
Anna Zaykovskaya ◽  
Anna Volynkina ◽  
...  

Background: Recently, a new multicomponent RNA-containing virus was described and called as Jingmen tick virus (JMTV) supposedly belonging to flaviviruses. A virus consists of four viral particles and JMTV was firstly isolated from ticks in China and South America. Aims: Detection viral RNA specific for JMTV complex, sequencing genome fragments and taxonomy identification novel virus from JMTV complex in patients with Crimean-Congo hemorrhagic fever (CCHF) from southern European part of Russia. Materials and methods: Panel of 20 randomly selected sera from patients with confirmed Crimean-Congo hemorrhagic fever was collected in 2016 and was used for detection JMTV and CCHF viral RNA by PCR with experimental primers. Subsequent sequencing of isolated fragments of viral genomes was used for identification JMTV and CCHF virus genetic materials and phylogenetic analyses. Results: The viral RNAs of the CCHF virus and JMTV were detected in blood of four patients. Sequencing of the isolated PCR fragment of S segment CCHF virus allowed identifying these RNA isolates as Europe 1 lineage, subgroups Va and Vb of the CCHF virus that is a typical for the southern European part of the Russia. The nucleotide sequences of segment 2 (GP glycoprotein) of the JMTV were also detected by RP PCR and sequencing in these human sera. The new JMTV isolates were clustered together by phylogenetic analysis. The level of nucleotide identity for newly discovered JMTV isolates was only about 81-82% with comparison to the previously described European variants (Kosovo) of the JMTV. Conclusions: The results suggest that viral genomic RNA for new multicomponent flavivirus named as Manych virus and related to the JMTV complex was discovered in sera of CCHF patients in Russia.


Author(s):  
N.F. Vasilenko ◽  
O.V. Maletskaya ◽  
E.A. Manin ◽  
D.A. Prislegina ◽  
L.I. Shaposhnikova ◽  
...  

The results of epizootological monitoring of the Southern European part of Russia (Southern and North- Caucasian Federal Districts), which has been tested for 18 nosological forms of natural focal infections are presented. There have been tested the laboratory studies of 45 615 samples of field material, the markers of pathogens of 15 nosological forms are identified. The circulation of Crimean-Congo hemorrhagic fever virus is identified in 10 subjects, the pathogen of tularemia – in eight regions, Lyme borreliosis – in seven, Q fever – in six; pathogens of hemorrhagic fever with renal syndrome, West Nile fever, leptospirosis and human granulocytic anaplasmosis – in five subjects. The markers of pathogens of human monocytic ehrlichiosis and tick-borne viral encephalitis were detected in four subjects, pseudotuberculosis and intestinal yersiniosis – in three and Arboviruses (Sindbis, Inkoо, Tahyna) – in one region.


Pathogens ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 540 ◽  
Author(s):  
Yuriy Davidyuk ◽  
Anton Shamsutdinov ◽  
Emmanuel Kabwe ◽  
Ruzilya Ismagilova ◽  
Ekaterina Martynova ◽  
...  

Puumala orthohantavirus (PUUV) causes nephropathia epidemica (NE), a mild form of hemorrhagic fever with renal syndrome (HFRS) commonly diagnosed in Europe. The majority of HFRS cases in the European part of Russia are diagnosed in the Volga Federal District, which includes the Republic of Tatarstan (RT). The current study aims to analyze the genetic variability of PUUV in Pre-Kama region of the RT bounded by the Volga, Kama, and Vyatka rivers. In 2017, bank voles were caught in seven isolated forest traps in the Pre-Kama region and for the 26 PUUV-positive samples, the partial small (S), medium (M), and large (L) genome segment sequences were obtained and analyzed. It was determined that all identified PUUV strains belong to the Russian (RUS) genetic lineage; however, the genetic distance between strains is not directly correlated with the geographical distance between bank vole populations. One of the identified strains has S and L segments produced from one parental strain, while the M segment was supplied by another, suggesting that this strain could be the reassortant. We suggest that the revealed pattern of the PUUV strains distribution could be the result of a series of successive multidirectional migratory flows of the bank voles to the Pre-Kama region in the postglacial period.


Author(s):  
O. V. Maletskaya ◽  
T. V. Taran ◽  
D. A. Prislegina ◽  
A. E. Platonov ◽  
V. M. Dubyansky ◽  
...  

Author(s):  
Lev V. Razumovsky

On the basis of author's graphical analysis method, the typification of lake ecosystems transformation scenarios depending on the size of lakes was carried out. It was confirmed that the type of transformation depends not only on size of the lake, but also on the landscape and climatic region in which it is located. The distinctive features of lake ecosystems transformation types in the European part of Russia and in Western Siberia were analyzed and compared.


2011 ◽  
pp. 101-116 ◽  
Author(s):  
B. Yu. Teteryuk

The results of a sintaxonomical study of plant communities of the Yamozero lake (the North-East of the European part of Russia) are presented. The diversity of the aquatic and helophytic vegetation of the Yamozero lake consists of 16 associations and 2 communities of 6 unions, 4 orders and 2 classes of the floristic classification: Potamogetonetea (7 associations, 2 communities), Phragmito-Magnocaricetea (9 associations). Many of described associations are widely distributed in the Central and the Eastern Europe. Some associations have the boundaries of their ranges. Some communities include 2 rare species of regional level: Isoetes setacea and Sagittaria natans.


2018 ◽  
Vol 52 (1) ◽  
pp. 91-100
Author(s):  
E. Yu. Blagoveshchenskaya

The paper provides the results of seven-year study of downy mildew on Skadovsky Zvenigorod Biological Station of Moscow State University (ZBS MSU, Moscow Region). A total of 29 species of Peronosporales (Oomycota) were revealed during the study. An annotated list of species is presented, among them Peronospora anemones is recorded for the first time for Russia, P. chelidonii and P. stachydis are new for the European part of Russia, 8 species are new for the Moscow Region.


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