scholarly journals Single-Cell Analysis of the Impact of Host Cell Heterogeneity on Infection with Foot-and-Mouth Disease Virus

2018 ◽  
Vol 92 (9) ◽  
pp. e00179-18 ◽  
Author(s):  
Xiu Xin ◽  
Hailong Wang ◽  
Lingling Han ◽  
Mingzhen Wang ◽  
Hui Fang ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Single Cell ◽  
Host Cell ◽  
Cell Size ◽  
Single Cell Analysis ◽  
Foot And Mouth Disease ◽  
Cell Analysis ◽  
Cell Heterogeneity ◽  
Mouth Disease Virus ◽  
Foot And Mouth

ABSTRACTViral infection and replication are affected by host cell heterogeneity, but the mechanisms underlying the effects remain unclear. Using single-cell analysis, we investigated the effects of host cell heterogeneity, including cell size, inclusion, and cell cycle, on foot-and-mouth disease virus (FMDV) infection (acute and persistent infections) and replication. We detected various viral genome replication levels in FMDV-infected cells. Large cells and cells with a high number of inclusions generated more viral RNA copies and viral protein and a higher proportion of infectious cells than other cells. Additionally, we found that the viral titer was 10- to 100-fold higher in cells in G2/M than those in other cell cycle phases and identified a strong correlation between cell size, inclusion, and cell cycle heterogeneity, which all affected the infection and replication of FMDV. Furthermore, we demonstrated that host cell heterogeneity influenced the adsorption of FMDV due to differences in the levels of FMDV integrin receptors expression. Collectively, these results further our understanding of the evolution of a virus in a single host cell.IMPORTANCEIt is important to understand how host cell heterogeneity affects viral infection and replication. Using single-cell analysis, we found that viral genome replication levels exhibited dramatic variability in foot-and-mouth disease virus (FMDV)-infected cells. We also found a strong correlation between heterogeneity in cell size, inclusion number, and cell cycle status and that all of these characteristics affect the infection and replication of FMDV. Moreover, we found that host cell heterogeneity influenced the viral adsorption as differences in the levels of FMDV integrin receptors' expression. This study provided new ideas for the studies of correlation between FMDV infection mechanisms and host cells.

scholarly journals Perturbations in the surface structure of A22 Iraq foot-and-mouth disease virus accompanying coupled changes in host cell specificity and antigenicity

Structure ◽  
1996 ◽  
Vol 4 (2) ◽  
pp. 135-145 ◽  
Author(s):  
Stephen Curry ◽  
Elizabeth Fry ◽  
Wendy Blakemore ◽  
Robin Abu Ghazaleh ◽  
Terry Jackson ◽  
...  
Keyword(s):  
Surface Structure ◽  
Host Cell ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Cell Specificity ◽  
Mouth Disease Virus ◽  
Foot And Mouth

scholarly journals Methods and sensors for functional genomic studies of cell-cycle transitions in single cells

2020 ◽  
Vol 52 (10) ◽  
pp. 468-477
Author(s):  
Alexander C. Zambon ◽  
Tom Hsu ◽  
Seunghee Erin Kim ◽  
Miranda Klinck ◽  
Jennifer Stowe ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Single Cell ◽  
Single Cell Analysis ◽  
Imaging System ◽  
Single Cells ◽  
Cell Types ◽  
Cell Analysis ◽  
Mcf7 Cells ◽  
Genomic Studies ◽  
Cell Subpopulations

Much of our understanding of the regulatory mechanisms governing the cell cycle in mammals has relied heavily on methods that measure the aggregate state of a population of cells. While instrumental in shaping our current understanding of cell proliferation, these approaches mask the genetic signatures of rare subpopulations such as quiescent (G0) and very slowly dividing (SD) cells. Results described in this study and those of others using single-cell analysis reveal that even in clonally derived immortalized cancer cells, ∼1–5% of cells can exhibit G0 and SD phenotypes. Therefore to enable the study of these rare cell phenotypes we established an integrated molecular, computational, and imaging approach to track, isolate, and genetically perturb single cells as they proliferate. A genetically encoded cell-cycle reporter (K67p-FUCCI) was used to track single cells as they traversed the cell cycle. A set of R-scripts were written to quantify K67p-FUCCI over time. To enable the further study G0 and SD phenotypes, we retrofitted a live cell imaging system with a micromanipulator to enable single-cell targeting for functional validation studies. Single-cell analysis revealed HT1080 and MCF7 cells had a doubling time of ∼24 and ∼48 h, respectively, with high duration variability in G1 and G2 phases. Direct single-cell microinjection of mRNA encoding (GFP) achieves detectable GFP fluorescence within ∼5 h in both cell types. These findings coupled with the possibility of targeting several hundreds of single cells improves throughput and sensitivity over conventional methods to study rare cell subpopulations.

scholarly journals Host Cell Selection of Antigenic Variants of Foot-and-Mouth Disease Virus

1989 ◽  
Vol 70 (1) ◽  
pp. 45-57 ◽  
Author(s):  
C. Bolwell ◽  
A. L. Brown ◽  
P. V. Barnett ◽  
R. O. Campbell ◽  
B. E. Clarke ◽  
...  
Keyword(s):  
Host Cell ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Cell Selection ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Selection Of

scholarly journals Foot-and-mouth disease virus protease 3C induces specific proteolytic cleavage of host cell histone H3.

1990 ◽  
Vol 64 (2) ◽  
pp. 748-756 ◽  
Author(s):  
M M Falk ◽  
P R Grigera ◽  
I E Bergmann ◽  
A Zibert ◽  
G Multhaup ◽  
...  
Keyword(s):  
Host Cell ◽  
Disease Virus ◽  
Histone H3 ◽  
Foot And Mouth Disease ◽  
Proteolytic Cleavage ◽  
Mouth Disease ◽  
Mouth Disease Virus ◽  
Foot And Mouth

scholarly journals Single-cell analyses uncover granularity of muscle stem cells

F1000Research ◽  
2020 ◽  
Vol 9 ◽  
pp. 31 ◽  
Author(s):  
John Saber ◽  
Alexander Y.T. Lin ◽  
Michael A. Rudnicki
Keyword(s):  
Single Cell ◽  
Cell Fate ◽  
Satellite Cells ◽  
Muscle Regeneration ◽  
Single Cell Analysis ◽  
Cell Analysis ◽  
Cell Heterogeneity ◽  
Muscle Stem Cells ◽  
Cell Fate Decisions ◽  
Cell Groups

Satellite cells are the main muscle-resident cells responsible for muscle regeneration. Much research has described this population as being heterogeneous, but little is known about the different roles each subpopulation plays. Recent advances in the field have utilized the power of single-cell analysis to better describe and functionally characterize subpopulations of satellite cells as well as other cell groups comprising the muscle tissue. Furthermore, emerging technologies are opening the door to answering as-yet-unresolved questions pertaining to satellite cell heterogeneity and cell fate decisions.

Sign in / Sign up

Export Citation Format

Share Document