Special peptidyl-tRNA molecules can promote translational frameshifting without slippage.

Recently we described an unusual programmed +1 frameshift event in yeast retrotransposon Ty3. Frameshifting depends on the presence of peptidyl-tRNA(AlaCGC) on the GCG codon in the ribosomal P site and on a translational pause stimulated by the slowly decoded AGU codon. Frameshifting occurs on the sequence GCG-AGU-U by out-of-frame binding of a valyl-tRNA to GUU without slippage of peptidyl-tRNA(AlaCGC). This mechanism challenges the conventional understanding that frameshift efficiency must correlate with the ability of mRNA-bound tRNA to slip between cognate or near-cognate codons. Though frameshifting does not require slippery tRNAs, it does require special peptidyl-tRNAs. We show that overproducing a second isoacceptor whose anticodon had been changed to CGC eliminated frameshifting; peptidyl-tRNA(AlaCGC) must have a special capacity to induce +1 frameshifting in the adjacent ribosomal A site. In order to identify other special peptidyl-tRNAs, we tested the ability of each of the other 63 codons to replace GCG in the P site. We found no correlation between the ability to stimulate +1 frameshifting and the ability of the cognate tRNA to slip on the mRNA--several codons predicted to slip efficiently do not stimulate frameshifting, while several predicted not to slip do stimulate frameshifting. By inducing a severe translational pause, we identified eight tRNAs capable of inducing measurable +1 frameshifting, only four of which are predicted to slip on the mRNA. We conclude that in Saccharomyces cerevisiae, special peptidyl-tRNAs can induce frameshifting dependent on some characteristic(s) other than the ability to slip on the mRNA.

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Actualisation du catalogue des pteridophytes du Nord Ouest Algerien (region de Tlemcen). An update of the cheklist of pteridophytes from Nordwest Algeria (Tlemcen region)

Acta Botanica Malacitana ◽

10.24310/abm.v38i0.2638 ◽

2013 ◽

Vol 38 ◽

pp. 33-39 ◽

Cited By ~ 6

Author(s):

Boumediene Medjahdi ◽

Assia Ltreuch-Belarouci ◽

Rémy Prelli

Keyword(s):

Nous Avons ◽

Genetic Resources ◽

Threatened Species ◽

The Other ◽

Natural Distribution ◽

Ecological Characteristics ◽

Statutory Protection ◽

Ouest Algérien ◽

A Site ◽

Ressources Génétiques

Français. Un inventaire des ptéridophytes a été entrepris dans les forêts de la région de Tlemcen. L’inventaire de ces populations constitue une étape importante pour le développement des stratégies de conservation des ressources génétiques et de la diversité de ces populations sur l’ensemble de leur aire de distribution naturelle. Nous avons ainsi effectué le recensement et l’identification des fougères existantes dans la région de Tlemcen. Au total, plusieurs stations dont les caractéristiques écologiques diffèrent d’un site à un autre ont été prospectées, cela a permis l’identification de 26 taxons (dont 5 exceptionnellement rare). La création de réserves naturelles forestières renforcée par une protection réglementaire des espèces les plus menacées est nécessaire pour le maintien de ces communautés si particulières. English. An inventory of Pteridophyta was begun in the forests of the Tlemcen region. The inventory of these populations constitutes an important stage for the developement of the strategies of preservation of the genetic resources and the diversity of these populations on their whole area of natural distribution. We so made the inventory and the identification of the existing ferns in the region of Tlemcen. On the whole, several stations the ecological characteristics differ from a site in the other one were canvassed; they allowed the identification of 26 taxes (among which 5 exceptionally rare). The creation of forest nature reserves strengthened by a statutory protection of the most threatened species is necessary for the preservation of these particular communities.

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Recombination of Ty elements in yeast can be induced by a double-strand break.

Genetics ◽

10.1093/genetics/140.1.67 ◽

1995 ◽

Vol 140 (1) ◽

pp. 67-77 ◽

Cited By ~ 1

Author(s):

A Parket ◽

O Inbar ◽

M Kupiec

Keyword(s):

Saccharomyces Cerevisiae ◽

Cell Cycle ◽

Strand Break ◽

Double Strand Break ◽

The Other ◽

Direct Repeats ◽

Yeast Saccharomyces Cerevisiae ◽

Low Frequencies ◽

Ty Elements ◽

Main Family

Abstract The Ty retrotransposons are the main family of dispersed repeated sequences in the yeast Saccharomyces cerevisiae. These elements are flanked by a pair of long terminal direct repeats (LTRs). Previous experiments have shown that Ty elements recombine at low frequencies, despite the fact that they are present in 30 copies per genome. This frequency is not highly increased by treatments that cause DNA damage, such as UV irradiation. In this study, we show that it is possible to increase the recombination level of a genetically marked Ty by creating a double-strand break in it. This break is repaired by two competing mechanisms: one of them leaves a single LTR in place of the Ty, and the other is a gene conversion event in which the marked Ty is replaced by an ectopically located one. In a strain in which the marked Ty has only one LTR, the double-strand break is repaired by conversion. We have also measured the efficiency of repair and monitored the progression of the cells through the cell-cycle. We found that in the presence of a double-strand break in the marked Ty, a proportion of the cells is unable to resume growth.

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Upstream – News in Genomics

Comparative and Functional Genomics ◽

10.1002/cfg.172 ◽

2002 ◽

Vol 3 (3) ◽

pp. 221-225

Keyword(s):

Saccharomyces Cerevisiae ◽

Ovarian Cancer ◽

Oryza Sativa ◽

Schizosaccharomyces Pombe ◽

Fission Yeast ◽

Proteomic Analysis ◽

Large Scale ◽

Protein Complexes ◽

The Other ◽

Blood Samples

In recent months a bumper crop of genomes has been completed, including the fission yeast (Schizosaccharomyces pombe) and rice (Oryza sativa). Two large-scale studies ofSaccharomyces cerevisiaeprotein complexes provided a picture of the eukaryotic proteome as a network of complexes. Amongst the other stories of interest was a demonstration that proteomic analysis of blood samples can be used to detect ovarian cancer, perhaps even as early as stage I.

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β-Li0.37Na0.63Fe(MoO4)2

Acta Crystallographica Section E Structure Reports Online ◽

10.1107/s1600536814000646 ◽

2014 ◽

Vol 70 (2) ◽

pp. i9-i10 ◽

Cited By ~ 6

Author(s):

Amira Souilem ◽

Mohamed Faouzi Zid ◽

Ahmed Driss

Keyword(s):

Crystal Structure ◽

Solid State ◽

Solid State Reaction ◽

Title Compound ◽

General Position ◽

Site Occupancy ◽

The Other ◽

Main Structure ◽

Axis Direction ◽

A Site

The title compound, lithium/sodium iron(III) bis[orthomolybdate(VI)], was obtained by a solid-state reaction. The main structure units are an FeO6octahedron, a distorted MoO6octahedron and an MoO4tetrahedron sharing corners. The crystal structure is composed of infinite double MoFeO11chains along theb-axis direction linked by corner-sharing to MoO4tetrahedra so as to form Fe2Mo3O19ribbons. The cohesion between ribbonsviamixed Mo—O—Fe bridges leads to layers arranged parallel to thebcplane. Adjacent layers are linked by corners shared between MoO4tetrahedra of one layer and FeO6octahedra of the other layer. The Na+and Li+ions partially occupy the same general position, with a site-occupancy ratio of 0.631 (9):0.369 (1). A comparison is made withAFe(MoO4)2(A= Li, Na, K and Cs) structures.

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The Cloning and Mapping of ADR6, a Gene Required for Sporulation and for Expression of the Alcohol Dehydrogenase II Isozyme From Saccharomyces cerevisiae

Genetics ◽

10.1093/genetics/116.4.531 ◽

1987 ◽

Vol 116 (4) ◽

pp. 531-540

Author(s):

Aileen K W Taguchi ◽

Elton T Young

Keyword(s):

Saccharomyces Cerevisiae ◽

Alcohol Dehydrogenase ◽

Single Gene ◽

Carbon Sources ◽

Transcription Unit ◽

Yeast Cells ◽

Recessive Mutation ◽

Yeast Saccharomyces Cerevisiae ◽

Upstream Activating Sequence ◽

A Site

ABSTRACT The alcohol dehydrogenase II (ADH2) gene of the yeast, Saccharomyces cerevisiae, is not transcribed during growth on fermentable carbon sources such as glucose. Growth of yeast cells in a medium containing only nonfermentable carbon sources leads to a marked increase or derepression of ADH2 expression. The recessive mutation, adr6-1, leads to an inability to fully derepress ADH2 expression and to an inability to sporulate. The ADR6 gene product appears to act directly or indirectly on ADH2 sequences 3' to or including the presumptive TATAA box. The upstream activating sequence (UAS) located 5' to the TATAA box is not required for the Adr6- phenotype. Here, we describe the isolation of a recombinant plasmid containing the wild-type ADR6 gene. ADR6 codes for a 4.4-kb RNA which is present during growth both on glucose and on nonfermentable carbon sources. Disruption of the ADR6 transcription unit led to viable cells with decreased ADHII activity and an inability to sporulate. This indicates that both phenotypes result from mutations within a single gene and that the adr6-1 allele was representative of mutations at this locus. The ADR6 gene mapped to the left arm of chromosome XVI at a site 18 centimorgans from the centromere.

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Two separate regions of the extrachromosomal ribosomal deoxyribonucleic acid of Tetrahymena thermophila enable autonomous replication of plasmids in Saccharomyces cerevisiae

Molecular and Cellular Biology ◽

10.1128/mcb.1.6.535-543.1981 ◽

1981 ◽

Vol 1 (6) ◽

pp. 535-543

Author(s):

G B Kiss ◽

A A Amin ◽

R E Pearlman

Keyword(s):

Saccharomyces Cerevisiae ◽

High Frequency ◽

Deoxyribonucleic Acid ◽

Tetrahymena Thermophila ◽

The Other ◽

Yeast Cells ◽

Origin Of Replication ◽

Autonomous Replication ◽

Coding Regions ◽

Dna Fragment

Plasmids containing the nontranscribed central and terminal, but not the coding, regions of the extrachromosomal ribosomal deoxyribonucleic acid (rDNA) of Tetrahymena thermophila are capable of autonomous replication in Saccharomyces cerevisiae. These plasmids transform S. cerevisiae at high frequency; transformants are unstable in the absence of selection, and plasmids identical to those used for transformation were isolated from the transformed yeast cells. One plasmid contains a 1.85-kilobase Tetrahymena DNA fragment which includes the origin of bidirectional replication of the extrachromosomal rDNA. The other region of Tetrahymena rDNA allowing autonomous replication of plasmids in S. cerevisiae is a 650-base pair, adenine plus thymine-rich segment from the rDNA terminus. Neither of these Tetrahymena fragments shares obvious sequence homology with the origin of replication of the S. cerevisiae 2-microns circle plasmid or with ars1, an S. cerevisiae chromosomal replicator.

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The HML mating-type cassette of Saccharomyces cerevisiae is regulated by two separate but functionally equivalent silencers

Molecular and Cellular Biology ◽

10.1128/mcb.9.11.4621-4630.1989 ◽

1989 ◽

Vol 9 (11) ◽

pp. 4621-4630

Author(s):

D J Mahoney ◽

J R Broach

Keyword(s):

Saccharomyces Cerevisiae ◽

Mating Type ◽

Binding Sites ◽

The Other ◽

Functional Domains ◽

Gene Products ◽

Cis Acting ◽

Full Expression ◽

Mating Type Genes ◽

Chromosome Iii

Mating-type genes resident in the silent cassette HML at the left arm of chromosome III are repressed by the action of four SIR gene products, most likely mediated through two cis-acting sites located on opposite sides of the locus. We showed that deletion of either of these two cis-acting sites from the chromosome did not yield any detectable derepression of HML, while deletion of both sites yielded full expression of the locus. In addition, each of these sites was capable of exerting repression of heterologous genes inserted in their vicinity. Thus, HML expression is regulated by two independent silencers, each fully competent for maintaining repression. This situation was distinct from the organization of the other silent locus, HMR, at which a single silencer served as the predominant repressor of expression. Examination of identifiable domains and binding sites within the HML silencers suggested that silencing activity can be achieved by a variety of combinations of various functional domains.

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SPT10 and SPT21 are required for transcription of particular histone genes in Saccharomyces cerevisiae

Molecular and Cellular Biology ◽

10.1128/mcb.14.8.5223-5228.1994 ◽

1994 ◽

Vol 14 (8) ◽

pp. 5223-5228

Author(s):

C Dollard ◽

S L Ricupero-Hovasse ◽

G Natsoulis ◽

J D Boeke ◽

F Winston

Keyword(s):

Saccharomyces Cerevisiae ◽

Differential Expression ◽

Double Mutant ◽

The Other ◽

Histone Genes ◽

Related Gene ◽

Histone Proteins ◽

Histone Locus

The Saccharomyces cerevisiae genome contains four loci that encode histone proteins. Two of these loci, HTA1-HTB1 and HTA2-HTB2, each encode histones H2A and H2B. The other two loci, HHT1-HHF1 and HHT2-HHF2, each encode histones H3 and H4. Because of their redundancy, deletion of any one histone locus does not cause lethality. Previous experiments demonstrated that mutations at one histone locus, HTA1-HTB1, do cause lethality when in conjunction with mutations in the SPT10 gene. SPT10 has been shown to be required for normal levels of transcription of several genes in S. cerevisiae. Motivated by this double-mutant lethality, we have now investigated the interactions of mutations in SPT10 and in a functionally related gene, SPT21, with mutations at each of the four histone loci. These experiments have demonstrated that both SPT10 and SPT21 are required for transcription at two particular histone loci, HTA2-HTB2 and HHF2-HHT2, but not at the other two histone loci. These results suggest that under some conditions, S. cerevisiae may control the level of histone proteins by differential expression of its histone genes.

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