scholarly journals The Drosophila Selenoprotein BthD Is Required for Survival and Has a Role in Salivary Gland Development

2003 ◽  
Vol 23 (23) ◽  
pp. 8495-8504 ◽  
Author(s):  
So Yeon Kwon ◽  
Paul Badenhorst ◽  
F. Javier Martin-Romero ◽  
Bradley A. Carlson ◽  
Bruce M. Paterson ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Molecular Level ◽  
Developmental Expression ◽  
Protein Levels ◽  
Gland Morphogenesis ◽  
Gland Development ◽  
Genomic Cdna ◽  
Activation Sequence ◽  
Inducible Gene

ABSTRACT Selenium is implicated in many diseases, including cancer, but its function at the molecular level is poorly understood. BthD is one of three selenoproteins recently identified in Drosophila. To elucidate the function of BthD and the role of selenoproteins in cellular metabolism and health, we analyzed the developmental expression profile of this protein and used inducible RNA interference (RNAi) to ablate function. We find that BthD is dynamically expressed during Drosophila development. bthD mRNA and protein are abundant in the ovaries of female flies and are deposited into the developing oocyte. Maternally contributed protein and RNA persist during early embryonic development but decay by the onset of gastrulation. At later stages of embryogenesis, BthD is expressed highly in the developing salivary gland. We generated transgenic fly lines carrying an inducible gene-silencing construct, in which an inverted bthD genomic-cDNA hybrid is under the control of the Drosophila Gal4 upstream activation sequence system. Duplex RNAi induced from this construct targeted BthD mRNA for destruction and reduced BthD protein levels. We found that loss of BthD compromised salivary gland morphogenesis and reduced animal viability.

scholarly journals 06-P040 The role of Eda signalling in salivary gland development

2009 ◽  
Vol 126 ◽  
pp. S131-S132
Author(s):  
Kirsty Wells ◽  
Denis Headon ◽  
Abigail Tucker
Keyword(s):  
Salivary Gland ◽  
Gland Development

scholarly journals Insights into the role of connexins in mammary gland morphogenesis and function

Reproduction ◽  
2015 ◽  
Vol 149 (6) ◽  
pp. R279-R290 ◽  
Author(s):  
Michael K G Stewart ◽  
Jamie Simek ◽  
Dale W Laird
Keyword(s):  
Mammary Gland ◽  
Gap Junctions ◽  
Normal Tissue ◽  
Mammary Gland Development ◽  
Mammary Gland Morphogenesis ◽  
Gland Morphogenesis ◽  
Cell Death Mechanisms ◽  
And Function ◽  
Gland Development

Gap junctions formed of connexin subunits link adjacent cells by direct intercellular communication that is essential for normal tissue homeostasis in the mammary gland. The mammary gland undergoes immense remodeling and requires exquisite regulation to control the proliferative, differentiating, and cell death mechanisms regulating gland development and function. The generation of novel genetically modified mice with reduced or ablated connexin function within the mammary gland has advanced our understanding of the role of gap junctions during the complex and dynamic process of mammary gland development. These studies have revealed an important stage-specific role for Cx26 (GJA1) and Cx43 (GJB2), while Cx30 (GJB6) and Cx32 (Gjb1) can be eliminated without compromising the gland. Yet, there remain gaps in our understanding of the role of mammary gland gap junctions.

scholarly journals Role of the mTOR signalling pathway in salivary gland development

FEBS Journal ◽  
2019 ◽  
Vol 286 (18) ◽  
pp. 3701-3717
Author(s):  
Manabu Sakai ◽  
Moe Fukumoto ◽  
Kazuki Ikai ◽  
Hitomi Ono Minagi ◽  
Shinobu Inagaki ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Signalling Pathway ◽  
Gland Development ◽  
Mtor Signalling ◽  
Mtor Signalling Pathway

The Role of Extracellular Matrix in the Morphogenesis and Differentiation of Salivary Glands

1990 ◽  
Vol 4 (1) ◽  
pp. 27-33 ◽  
Author(s):  
L.S. Cutler
Keyword(s):  
Extracellular Matrix ◽  
Salivary Gland ◽  
Type Iv Collagen ◽  
The Body ◽  
Secretory Cells ◽  
Type Iv ◽  
Preliminary Information ◽  
Extracellular Matrix Molecules ◽  
Gland Morphogenesis

The processes of morphogenesis and cytodifferentiation are partially linked, independently regulated processes. The full expression of both processes is modulated or controlled, at least in part, by components of the extracellular matrix. This paper reviews the body of work that demonstrates a role for epithelial-mesenchymal interactions and various extracellular matrix molecules in the induction, control, and maintenance of salivary gland morphogenesis and cytodifferentiation. In addition, new, preliminary information which further elucidates the role of laminin and type IV collagen in the processes of morphogenesis and cytodifferentiation is presented. With regard to the role of extracellular matrix molecules in the regulation of salivary gland morphogenesis and cytodifferentiation, it appears that types I, III, and IV collagen, laminin, and chondroitin sulfate proteoglycan play roles in the control of glandular morphogenesis. With the exception of type IV collagen, these molecules do not appear to be involved in the regulation of cytodifferentiation of salivary gland secretory cells. On the other hand, of the extracellular matrix molecules tested so far, only type IV collagen appears to play a role in the regulation of salivary gland secretory cell differentiation.

scholarly journals AnIn VitroCulture System for Long-Term Expansion of Epithelial and Mesenchymal Salivary Gland Cells: Role of TGF-β1 in Salivary Gland Epithelial and Mesenchymal Differentiation

2013 ◽  
Vol 2013 ◽  
pp. 1-20 ◽  
Author(s):  
Kajohnkiart Janebodin ◽  
Worakanya Buranaphatthana ◽  
Nicholas Ieronimakis ◽  
Aislinn L. Hays ◽  
Morayma Reyes
Keyword(s):  
Salivary Gland ◽  
Culture System ◽  
Gland Cell ◽  
Mesenchymal Cells ◽  
Salivary Gland Cell ◽  
Salivary Gland Cells ◽  
Gland Development

Despite a pivotal role in salivary gland development, homeostasis, and disease, the role of salivary gland mesenchyme is not well understood. In this study, we used theCol1a1-GFPmouse model to characterize the salivary gland mesenchymein vitroandin vivo. TheCol1a1-GFPtransgene was exclusively expressed in the salivary gland mesenchyme.Ex vivoculture of mixed salivary gland cells in DMEM plus serum medium allowed long-term expansion of salivary gland epithelial and mesenchymal cells. The role of TGF-β1 in salivary gland development and disease is complex. Therefore, we used thisin vitroculture system to study the effects of TGF-β1 on salivary gland cell differentiation. TGF-β1 induced the expression of collagen, and inhibited the formation of acini-like structures in close proximity to mesenchymal cells, which adapted a fibroblastic phenotype. In contrast, TGF-βR1 inhibition increased acini genes and fibroblast growth factors (Fgf-7andFgf-10), decreased collagen and induced formation of larger, mature acini-like structures. Thus, inhibition of TGF-βsignaling may be beneficial for salivary gland differentiation; however, due to differential effects of TGF-β1 in salivary gland epithelial versus mesenchymal cells, selective inhibition is desirable. In conclusion, this mixed salivary gland cell culture system can be used to study epithelial-mesenchymal interactions and the effects of differentiating inducers and inhibitors.

scholarly journals BAD regulates mammary gland morphogenesis by 4E-BP1-mediated control of localized translation in mouse and human models

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
John Maringa Githaka ◽  
Namita Tripathi ◽  
Raven Kirschenman ◽  
Namrata Patel ◽  
Vrajesh Pandya ◽  
...  
Keyword(s):  
Cell Migration ◽  
Mammary Gland ◽  
Mammary Gland Development ◽  
Focal Adhesions ◽  
Mrna Translation ◽  
Protein Functions ◽  
Mammary Gland Morphogenesis ◽  
Gland Morphogenesis ◽  
Gland Development

AbstractElucidation of non-canonical protein functions can identify novel tissue homeostasis pathways. Herein, we describe a role for the Bcl-2 family member BAD in postnatal mammary gland morphogenesis. In Bad3SA knock-in mice, where BAD cannot undergo phosphorylation at 3 key serine residues, pubertal gland development is delayed due to aberrant tubulogenesis of the ductal epithelium. Proteomic and RPPA analyses identify that BAD regulates focal adhesions and the mRNA translation repressor, 4E-BP1. These results suggest that BAD modulates localized translation that drives focal adhesion maturation and cell motility. Consistent with this, cells within Bad3SA organoids contain unstable protrusions with decreased compartmentalized mRNA translation and focal adhesions, and exhibit reduced cell migration and tubulogenesis. Critically, protrusion stability is rescued by 4E-BP1 depletion. Together our results confirm an unexpected role of BAD in controlling localized translation and cell migration during mammary gland development.

scholarly journals Epithelial-Mesenchymal Interactions in the Development of Salivary Glands

1991 ◽  
Vol 2 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Leslie S. Cutler ◽  
Waldemiro Gremski
Keyword(s):  
Extracellular Matrix ◽  
Salivary Gland ◽  
Cell Differentiation ◽  
Salivary Glands ◽  
Secretory Cell ◽  
Full Expression ◽  
Extracellular Matrix Molecules ◽  
Gland Morphogenesis

The full expression of both morphogenesis and secretory cell differentiation in salivary glands is modulated or controlled, at least in part, by interactions between the salivary epithelium and the surrounding mesenchyme. Salivary gland morphogenesis and cytodifferentiation are partially linked but independently regulated processes. This presentation reviews the information that establishes the role of various extracellular matrix molecules and direct epithelial-mesenchymal interactions in the induction, control, and maintenance of morphogenesis and secretory cell differentiation in salivary glands.

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