Genome Sequence of Serratia fonticola Strain S14, Isolated from the Mosquito Aedes triseriatus

ABSTRACT Here, we present the 3.9-Mb draft genome sequence of Nitrobacter vulgaris strain Ab1, which was isolated from a sewage system in Hamburg, Germany. The analysis of its genome sequence will contribute to our knowledge of nitrite-oxidizing bacteria and acyl-homoserine lactone quorum sensing in nitrifying bacteria.

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Complete Genome Sequence of Pseudomonas putida Strain TS312, Harboring an HdtS-Type N-Acyl-Homoserine Lactone Synthase, Isolated from a Paper Mill

Microbiology Resource Announcements ◽

10.1128/mra.00055-20 ◽

2020 ◽

Vol 9 (13) ◽

Author(s):

Ayaka Hosoe ◽

Toshikazu Suenaga ◽

Takumi Sugi ◽

Taro Iizumi ◽

Naohiro Nagai ◽

...

Keyword(s):

Pseudomonas Putida ◽

Genome Sequence ◽

Complete Genome Sequence ◽

Complete Genome ◽

Paper Mill ◽

Homoserine Lactone ◽

Acyl Homoserine Lactone ◽

Gene Encoding ◽

Content Type ◽

Engineered Systems

We report the complete genome sequence of Pseudomonas putida strain TS312, in the class of Gammaproteobacteria. The strain, isolated from a paper mill, harbors the hdtS gene, encoding N-acyl-homoserine lactone synthase. Deciphering the genome contributes to revealing the mechanisms of quorum sensing and associated biofilm formation in engineered systems.

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Disruption of Quorum Sensing and Virulence inBurkholderia cenocepaciaby a Structural Analogue of thecis-2-Dodecenoic Acid Signal

Applied and Environmental Microbiology ◽

10.1128/aem.00105-19 ◽

2019 ◽

Vol 85 (8) ◽

Cited By ~ 4

Author(s):

Chaoyu Cui ◽

Shihao Song ◽

Chunxi Yang ◽

Xiuyun Sun ◽

Yutong Huang ◽

...

Keyword(s):

Quorum Sensing ◽

Bacterial Infections ◽

Opportunistic Pathogen ◽

Homoserine Lactone ◽

Burkholderia Cenocepacia ◽

Enoic Acid ◽

Acyl Homoserine Lactone ◽

Structural Analogue ◽

Content Type ◽

Signal Production

ABSTRACTQuorum sensing (QS) signals are widely used by bacterial pathogens to control biological functions and virulence in response to changes in cell population densities.Burkholderia cenocepaciaemploys a molecular mechanism in which thecis-2-dodecenoic acid (namedBurkholderiadiffusiblesignalfactor [BDSF]) QS system regulatesN-acyl homoserine lactone (AHL) signal production and virulence by modulating intracellular levels of cyclic diguanosine monophosphate (c-di-GMP). Thus, inhibition of BDSF signaling may offer a non-antibiotic-based therapeutic strategy against BDSF-regulated bacterial infections. In this study, we report the synthesis of small-molecule mimics of the BDSF signal and evaluate their ability to inhibit BDSF QS signaling inB. cenocepacia. A novel structural analogue of BDSF, 14-Me-C16:Δ2(cis-14-methylpentadec-2-enoic acid), was observed to inhibit BDSF production and impair BDSF-regulated phenotypes inB. cenocepacia, including motility, biofilm formation, and virulence, while it did not inhibit the growth rate of this pathogen. 14-Me-C16:Δ2also reduced AHL signal production. Genetic and biochemical analyses showed that 14-Me-C16:Δ2inhibited the production of the BDSF and AHL signals by decreasing the expression of their synthase-encoding genes. Notably, 14-Me-C16:Δ2attenuated BDSF-regulated phenotypes in variousBurkholderiaspecies. These findings suggest that 14-Me-C16:Δ2could potentially be developed as a new therapeutic agent against pathogenicBurkholderiaspecies by interfering with their QS signaling.IMPORTANCEBurkholderia cenocepaciais an important opportunistic pathogen which can cause life-threatening infections in susceptible individuals, particularly in cystic fibrosis and immunocompromised patients. It usually employs two types of quorum sensing (QS) systems, including thecis-2-dodecenoic acid (BDSF) system andN-acyl homoserine lactone (AHL) system, to regulate virulence. In this study, we have designed and identified an unsaturated fatty acid compound (cis-14-methylpentadec-2-enoic acid [14-Me-C16:Δ2]) that is capable of interfering withB. cenocepaciaQS signaling and virulence. We demonstrate that 14-Me-C16:Δ2reduced BDSF and AHL signal production inB. cenocepacia. It also impaired QS-regulated phenotypes in variousBurkholderiaspecies. These results suggest that 14-Me-C16:Δ2could interfere with QS signaling in manyBurkholderiaspecies and might be developed as a new antibacterial agent.

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Draft Genome Sequence of Lactobacillus paraplantarum OSY-TC318, a Producer of the Novel Lantibiotic Paraplantaracin TC318

Microbiology Resource Announcements ◽

10.1128/mra.00274-19 ◽

2019 ◽

Vol 8 (19) ◽

Cited By ~ 2

Author(s):

Walaa E. Hussein ◽

En Huang ◽

Ismet Ozturk ◽

Ahmed E. Yousef

Keyword(s):

Genome Size ◽

Genome Sequence ◽

Draft Genome ◽

Gc Content ◽

Peptide Sequencing ◽

Draft Genome Sequence ◽

The Novel ◽

Content Type ◽

A Genome ◽

Lactobacillus Paraplantarum

Lactobacillus paraplantarum OSY-TC318 was isolated from Turkish Tulum cheese and found to produce a potent anti-Gram-positive peptide. Sequencing of the OSY-TC318 genome revealed a genome size of 3,587,488 bp and an average GC content of 43.4%.

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Acyl-Homoserine Lactone Production in Nitrifying Bacteria of the Genera Nitrosospira, Nitrobacter, and Nitrospira Identified via a Survey of Putative Quorum-Sensing Genes

Applied and Environmental Microbiology ◽

10.1128/aem.01540-17 ◽

2017 ◽

Vol 83 (22) ◽

Cited By ~ 29

Author(s):

Brett L. Mellbye ◽

Eva Spieck ◽

Peter J. Bottomley ◽

Luis A. Sayavedra-Soto

Keyword(s):

Gene Regulation ◽

Quorum Sensing ◽

Nitrogen Oxide ◽

Nitrogen Cycling ◽

Bacterial Cell ◽

Homoserine Lactone ◽

Nitrifying Bacteria ◽

Acyl Homoserine Lactone ◽

Content Type ◽

Bacterial Communication

ABSTRACT The genomes of many bacteria that participate in nitrogen cycling through the process of nitrification contain putative genes associated with acyl-homoserine lactone (AHL) quorum sensing (QS). AHL QS or bacterial cell-cell signaling is a method of bacterial communication and gene regulation and may be involved in nitrogen oxide fluxes or other important phenotypes in nitrifying bacteria. Here, we carried out a broad survey of AHL production in nitrifying bacteria in three steps. First, we analyzed the evolutionary history of AHL synthase and AHL receptor homologs in sequenced genomes and metagenomes of nitrifying bacteria to identify AHL synthase homologs in ammonia-oxidizing bacteria (AOB) of the genus Nitrosospira and nitrite-oxidizing bacteria (NOB) of the genera Nitrococcus, Nitrobacter, and Nitrospira. Next, we screened cultures of both AOB and NOB with uncharacterized AHL synthase genes and AHL synthase-negative nitrifiers by a bioassay. Our results suggest that an AHL synthase gene is required for, but does not guarantee, cell density-dependent AHL production under the conditions tested. Finally, we utilized mass spectrometry to identify the AHLs produced by the AOB Nitrosospira multiformis and Nitrosospira briensis and the NOB Nitrobacter vulgaris and Nitrospira moscoviensis as N-decanoyl-l-homoserine lactone (C10-HSL), N-3-hydroxy-tetradecanoyl-l-homoserine lactone (3-OH-C14-HSL), a monounsaturated AHL (C10:1-HSL), and N-octanoyl-l-homoserine lactone (C8-HSL), respectively. Our survey expands the list of AHL-producing nitrifiers to include a representative of Nitrospira lineage II and suggests that AHL production is widespread in nitrifying bacteria. IMPORTANCE Nitrification, the aerobic oxidation of ammonia to nitrate via nitrite by nitrifying microorganisms, plays an important role in environmental nitrogen cycling from agricultural fertilization to wastewater treatment. The genomes of many nitrifying bacteria contain genes associated with bacterial cell-cell signaling or quorum sensing (QS). QS is a method of bacterial communication and gene regulation that is well studied in bacterial pathogens, but less is known about QS in environmental systems. Our previous work suggested that QS might be involved in the regulation of nitrogen oxide gas production during nitrite metabolism. This study characterized putative QS signals produced by different genera and species of nitrifiers. Our work lays the foundation for future experiments investigating communication between nitrifying bacteria, the purpose of QS in these microorganisms, and the manipulation of QS during nitrification.

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Complete Genome Sequence of Adlercreutzia equolifaciens subsp. celatus DSM 18785

Microbiology Resource Announcements ◽

10.1128/mra.00354-21 ◽

2021 ◽

Vol 10 (19) ◽

Author(s):

Haruno Takahashi ◽

Jiayue Yang ◽

Hiromitsu Yamamoto ◽

Shinji Fukuda ◽

Kazuharu Arakawa

Keyword(s):

Genome Size ◽

Genome Sequence ◽

Complete Genome Sequence ◽

Complete Genome ◽

Content Type ◽

Obligately Anaerobic ◽

A Genome

ABSTRACT Adlercreutzia equolifaciens subsp. celatus DSM 18785 was isolated from the cecal contents of a rat and is an obligately anaerobic equol-producing bacterium. Here, we report the finished and annotated genome sequence of this organism, which has a genome size of 2,929,991 bp and a G+C content of 63.2%.

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ThePseudomonas aeruginosaOrphan Quorum Sensing Signal Receptor QscR Regulates Global Quorum Sensing Gene Expression by Activating a Single Linked Operon

mBio ◽

10.1128/mbio.01274-18 ◽

2018 ◽

Vol 9 (4) ◽

Cited By ~ 15

Author(s):

Fengming Ding ◽

Ken-Ichi Oinuma ◽

Nicole E. Smalley ◽

Amy L. Schaefer ◽

Omar Hamwy ◽

...

Keyword(s):

Gene Expression ◽

Pseudomonas Aeruginosa ◽

Transcription Factor ◽

Quorum Sensing ◽

Cell Communication ◽

Homoserine Lactone ◽

Acyl Homoserine Lactone ◽

Null Mutant ◽

Content Type ◽

Single Operon

ABSTRACTPseudomonas aeruginosauses two acyl-homoserine lactone signals and two quorum sensing (QS) transcription factors, LasR and RhlR, to activate dozens of genes. LasR responds toN-3-oxo-dodecanoyl-homoserine lactone (3OC12-HSL) and RhlR toN-butanoyl-homoserine lactone (C4-HSL). There is a thirdP. aeruginosaacyl-homoserine-lactone-responsive transcription factor, QscR, which acts to dampen or delay activation of genes by LasR and RhlR by an unknown mechanism. To better understand the role of QscR inP. aeruginosaQS, we performed a chromatin immunoprecipitation analysis, which showed this transcription factor bound the promoter of only a single operon of three genes linked toqscR, PA1895 to PA1897. Other genes that appear to be regulated by QscR in transcriptome studies were not direct targets of QscR. Deletion of PA1897 recapitulates the early QS activation phenotype of a QscR-null mutant, and the phenotype of a QscR-null mutant was complemented by PA1895-1897 but not by PA1897 alone. We conclude that QscR acts to modulate quorum sensing through regulation of a single operon, apparently raising the QS threshold of the population and providing a “brake” on QS autoinduction.IMPORTANCEQuorum sensing, a cell-cell communication system, is broadly distributed among bacteria and is commonly used to regulate the production of shared products. An important consequence of quorum sensing is a delay in production of certain products until the population density is high. The bacteriumPseudomonas aeruginosahas a particularly complicated quorum sensing system involving multiple signals and receptors. One of these receptors, QscR, downregulates gene expression, unlike the other receptors inP. aeruginosa. QscR does so by inducing the expression of a single operon whose function provides an element of resistance to a population reaching a quorum. This finding has importance for design of quorum sensing inhibitory strategies and can also inform design of synthetic biological circuits that use quorum sensing receptors to regulate gene expression.

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Draft Genome Sequence of the Salt Water Bacterium Oceanospirillum linum ATCC 11336T

Genome Announcements ◽

10.1128/genomea.00395-17 ◽

2017 ◽

Vol 5 (21) ◽

Cited By ~ 1

Author(s):

Ariel M. Trachtenberg ◽

Joshua G. Carney ◽

Joshua D. Linnane ◽

Bruce A. Rheaume ◽

Natalie L. Pitts ◽

...

Keyword(s):

Genome Size ◽

Genome Sequence ◽

Salt Water ◽

Long Island ◽

Draft Genome ◽

Long Island Sound ◽

Coding Sequences ◽

Content Type ◽

A Genome ◽

Island Sound

ABSTRACT Oceanospirillum linum ATCC 11336T is an aerobic, bipolar-tufted gammaproteobacterium first isolated in the Long Island Sound in the 1950s. This announcement offers a genome sequence for O. linum ATCC 11336T, which has a predicted genome size of 3,782,189 bp (49.13% G+C content) containing 3,540 genes and 3,361 coding sequences.

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Streptomycin Inhibits Quorum Sensing in Acinetobacter baumannii

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.02161-12 ◽

2013 ◽

Vol 57 (4) ◽

pp. 1926-1929 ◽

Cited By ~ 30

Author(s):

Sunil D. Saroj ◽

Philip N. Rather

Keyword(s):

Quorum Sensing ◽

Thin Layer ◽

Acinetobacter Baumannii ◽

Direct Analysis ◽

Homoserine Lactone ◽

Acyl Homoserine Lactone ◽

Content Type ◽

Signal Production ◽

Subinhibitory Concentrations ◽

Layer Chromatography

ABSTRACTStreptomycin at subinhibitory concentrations was found to inhibit quorum sensing inAcinetobacter baumannii. Conditioned medium prepared by growth ofA. baumanniiin the presence of subinhibitory concentrations of streptomycin exhibited reduced activation of two quorum-sensing-regulated genes,abaI, encoding an autoinducer synthase, and A1S_0112. The reduced expression of AbaI resulted in greatly decreased levels of 3-OH-C12-HSL as confirmed by direct analysis using thin-layer chromatography. The effect on acyl-homoserine lactone (AHL) signal production was specific to streptomycin, as gentamicin and myomycin had no significant effect at subinhibitory levels.

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Genome Sequence of Novosphingobium sp. Strain Rr 2-17, a Nopaline Crown Gall-Associated Bacterium Isolated from Vitis vinifera L. Grapevine

Journal of Bacteriology ◽

10.1128/jb.01159-12 ◽

2012 ◽

Vol 194 (18) ◽

pp. 5137-5138 ◽

Cited By ~ 10

Author(s):

Han Ming Gan ◽

Teong Han Chew ◽

André O. Hudson ◽

Michael A. Savka

Keyword(s):

Vitis Vinifera ◽

Genome Sequence ◽

Crown Gall ◽

Draft Genome ◽

Homoserine Lactone ◽

Vitis Vinifera L ◽

Acyl Homoserine Lactone ◽

Content Type ◽

Crown Gall Tumor

ABSTRACTNovosphingobiumsp. strain Rr 2-17 is anN-acyl homoserine lactone (AHL)-producing bacterium isolated from the crown gall tumor of a grapevine. To our knowledge, this is the first draft genome announcement of a plant-associated strain from the genusNovosphingobium.

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