scholarly journals Complete Genome Sequences of Four Putatively Antibiotic-Producing Bacteria Isolated from Soil in Arkansas, USA

2022 ◽  
Author(s):  
Ruth Plymale ◽  
Griffin Hopkins ◽  
Taylor Johnson ◽  
Taylor Savage ◽  
Danielle Schaal
Keyword(s):  
Complete Genome ◽  
Soil Bacteria ◽  
Hybrid Approach ◽  
Antimicrobial Compounds ◽  
Research Experience ◽  
Genome Sequences ◽  
Valuable Source ◽  
Oxford Nanopore ◽  
Complete Genomes ◽  
Oxford Nanopore Technologies

Soil bacteria can be a valuable source of antimicrobial compounds. Here, we report the complete genomes of four soil bacteria that were isolated by undergraduate microbiology students as part of a course-based research experience. These genomes were assembled using a hybrid approach combining paired-end Illumina reads with Oxford Nanopore Technologies MinION reads.

scholarly journals Complete Genome Sequences of Bordetella pertussis Clinical Isolate FR5810 and Reference Strain Tohama from Combined Oxford Nanopore and Illumina Sequencing

2018 ◽  
Vol 7 (19) ◽  
Author(s):  
Valérie Bouchez ◽  
Sarah Louise Baines ◽  
Sophie Guillot ◽  
Sylvain Brisse
Keyword(s):  
Clinical Isolate ◽  
Bordetella Pertussis ◽  
Complete Genome ◽  
Reference Strain ◽  
Sequence Data ◽  
Hybrid Approach ◽  
Genome Sequences ◽  
Content Type ◽  
Oxford Nanopore ◽  
Oxford Nanopore Technologies

Here, we describe the complete genome sequences of two Bordetella pertussis strains, FR5810, a clinical isolate recovered from the respiratory tract of an infant, and Tohama, a key reference strain for the species. Sequences were obtained using a hybrid approach combining Oxford Nanopore Technologies MinION and Illumina NextSeq 500 sequence data.

scholarly journals Complete Genome Sequences for Pseudomonas sp. Strains 29A and 43A

2020 ◽  
Vol 9 (39) ◽  
Author(s):  
David A. Baltrus ◽  
Meara Clark ◽  
Parris T. Humphrey ◽  
Noah K. Whiteman
Keyword(s):  
Complete Genome ◽  
Hybrid Approach ◽  
Flow Cell ◽  
Pseudomonas Sp ◽  
Genome Sequences ◽  
Content Type ◽  
Oxford Nanopore ◽  
Cardamine Cordifolia

ABSTRACT Pseudomonas sp. strains 29A and 43A were originally isolated from the phyllosphere of individual plants of Cardamine cordifolia (Brassicaceae). Here, we report complete genome sequences for these two closely related strains, assembled using a hybrid approach combining Illumina paired-end reads and longer reads sequenced on an Oxford Nanopore MinION flow cell.

scholarly journals Closed Genome Sequences of Clinical Neisseria gonorrhoeae Strains Obtained from Combined Oxford Nanopore and Illumina Sequencing

2019 ◽  
Vol 8 (9) ◽  
Author(s):  
Richard C. White ◽  
Manolito Torralba ◽  
Kelly Colt ◽  
Frank Harrison ◽  
Karrie Goglin ◽  
...  
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Hybrid Approach ◽  
Illumina Miseq ◽  
The United States ◽  
Sequencing Data ◽  
Notifiable Disease ◽  
Genome Sequences ◽  
Content Type ◽  
Oxford Nanopore ◽  
Oxford Nanopore Technologies

Neisseria gonorrhoeae is the etiological agent of gonorrhea, the second most common notifiable disease in the United States. Here, we used a hybrid approach combining Oxford Nanopore Technologies MinION and Illumina MiSeq sequencing data to obtain closed genome sequences of nine clinical N. gonorrhoeae isolates.

scholarly journals Complete Genome Sequences of Six Novel Macaca mulatta Papillomavirus Types Isolated from Genital Sites of Rhesus Monkeys in Hong Kong SAR, China

2018 ◽  
Vol 7 (22) ◽  
Author(s):  
Teng Long ◽  
Po Yee Wong ◽  
Wendy C. S. Ho ◽  
Robert D. Burk ◽  
Paul K. S. Chan ◽  
...  
Keyword(s):  
Hong Kong ◽  
Macaca Mulatta ◽  
Complete Genome ◽  
Rhesus Monkeys ◽  
Genome Sequences ◽  
Content Type ◽  
Complete Genomes ◽  
Hong Kong Sar

The complete genomes of six Macaca mulatta papillomavirus types isolated from genital sites of rhesus monkeys were characterized, and less than 72% identity with the complete L1 genes of known papillomaviruses was found. Macaca mulatta papillomavirus type 2 (MmPV2), MmPV3, and MmPV6 cluster into the genus Alphapapillomavirus, and MmPV4, MmPV5, and MmPV7 cluster into the genus Gammapapillomavirus.

scholarly journals Complete Genome Sequences of Five Burkholderia Strains with Biocontrol Activity against Various Lettuce Pathogens

2022 ◽  
Author(s):  
Adrien Biessy ◽  
Marie Ciotola ◽  
Mélanie Cadieux ◽  
Daphné Albert ◽  
Martin Filion
Keyword(s):  
Complete Genome ◽  
Burkholderia Cepacia ◽  
Gene Clusters ◽  
Antimicrobial Compounds ◽  
Bacterial Strains ◽  
Genome Sequences ◽  
Biosynthetic Gene Clusters ◽  
Content Type ◽  
Biocontrol Activity ◽  
Complex Display

Numerous bacterial strains from the Burkholderia cepacia complex display biocontrol activity. Here, we report the complete genome sequences of five Burkholderia strains isolated from soil. Biosynthetic gene clusters responsible for the production of antimicrobial compounds were found in the genome of these strains, which display biocontrol activity against various lettuce pathogens.

scholarly journals Complete Circular Genome Sequences of Three Bacillus cereus Group Strains Isolated from Positive Blood Cultures from Preterm and Immunocompromised Infants Hospitalized in France

2021 ◽  
Vol 10 (41) ◽  
Author(s):  
Mariem Ben Khedher ◽  
Fredrik Nindo ◽  
Alicia Chevalier ◽  
Stéphane Bonacorsi ◽  
Gregory Dubourg ◽  
...  
Keyword(s):  
Intensive Care ◽  
Bacillus Cereus ◽  
Complete Genome ◽  
Blood Cultures ◽  
Genome Sequences ◽  
Bacillus Cereus Group ◽  
Illumina Hiseq ◽  
Content Type ◽  
Long Reads ◽  
Oxford Nanopore

We report here the complete genome sequences of three Bacillus cereus group strains isolated from blood cultures from premature and immunocompromised infants hospitalized in intensive care units in three French hospitals. These complete genome sequences were obtained from a combination of Illumina HiSeq X Ten short reads and Oxford Nanopore MinION long reads.

scholarly journals Diversity of Pectobacteriaceae Species in Potato Growing Regions in Northern Morocco

2020 ◽  
Vol 8 (6) ◽  
pp. 895 ◽  
Author(s):  
Saïd Oulghazi ◽  
Mohieddine Moumni ◽  
Slimane Khayi ◽  
Kévin Robic ◽  
Sohaib Sarfraz ◽  
...  
Keyword(s):  
Species Diversity ◽  
Complete Genome ◽  
Draft Genome ◽  
Epidemiological Studies ◽  
Gene Marker ◽  
Oxford Nanopore ◽  
Causative Agents ◽  
Genome Analyses ◽  
Oxford Nanopore Technologies

Dickeya and Pectobacterium pathogens are causative agents of several diseases that affect many crops worldwide. This work investigated the species diversity of these pathogens in Morocco, where Dickeya pathogens have only been isolated from potato fields recently. To this end, samplings were conducted in three major potato growing areas over a three-year period (2015–2017). Pathogens were characterized by sequence determination of both the gapA gene marker and genomes using Illumina and Oxford Nanopore technologies. We isolated 119 pathogens belonging to P. versatile (19%), P. carotovorum (3%), P. polaris (5%), P. brasiliense (56%) and D. dianthicola (17%). Their taxonomic assignation was confirmed by draft genome analyses of 10 representative strains of the collected species. D. dianthicola were isolated from a unique area where a wide species diversity of pectinolytic pathogens was observed. In tuber rotting assays, D. dianthicola isolates were more aggressive than Pectobacterium isolates. The complete genome sequence of D. dianthicola LAR.16.03.LID was obtained and compared with other D. dianthicola genomes from public databases. Overall, this study highlighted the ecological context from which some Dickeya and Pectobacterium species emerged in Morocco, and reported the first complete genome of a D. dianthicola strain isolated in Morocco that will be suitable for further epidemiological studies.

scholarly journals Complete Genome Sequence of the Type Strain Pectobacterium punjabense SS95, Isolated from a Potato Plant with Blackleg Symptoms

2020 ◽  
Vol 9 (32) ◽  
Author(s):  
Sohaib Sarfraz ◽  
Saïd Oulghazi ◽  
Jérémy Cigna ◽  
Shahbaz Talib Sahi ◽  
Kashif Riaz ◽  
...  
Keyword(s):  
Complete Genome ◽  
Type Strain ◽  
Gc Content ◽  
Illumina Miseq ◽  
Blackleg Disease ◽  
Circular Chromosome ◽  
Content Type ◽  
Potato Plants ◽  
Oxford Nanopore ◽  
Oxford Nanopore Technologies

ABSTRACT Pectobacterium punjabense is a newly described species causing blackleg disease in potato plants. Therefore, by the combination of long (Oxford Nanopore Technologies, MinION) and short (Illumina MiSeq) reads, we sequenced the complete genome of P. punjabense SS95T, which contains a circular chromosome of 4.793 Mb with a GC content of 50.7%.

scholarly journals Complete Genome Sequences of Two Human Oral Microbiome Commensals: Streptococcus salivarius ATCC 25975 and S. salivarius ATCC 27945

2017 ◽  
Vol 5 (24) ◽  
Author(s):  
Robert R. Butler ◽  
Jahna T. A. Soomer-James ◽  
Michel Frenette ◽  
Jean-François Pombert
Keyword(s):  
Complete Genome ◽  
Oral Microbiome ◽  
Streptococcus Salivarius ◽  
Genome Sequences ◽  
Content Type ◽  
Complete Genomes

ABSTRACT Streptococcus salivarius strains are significant contributors to the human oral microbiome. Some possess unique fimbriae that give them the ability to coaggregate and colonize particular oral structures. We present here the complete genomes of Streptococcus salivarius Lancefield K−/K+ strains ATCC 25975 and ATCC 27945, which can and cannot, respectively, produce fimbriae.

scholarly journals Complete Genome Sequences of Vibrio cholerae-Specific Bacteriophages 24 and X29

2017 ◽  
Vol 5 (46) ◽  
Author(s):  
Sudhakar G. Bhandare ◽  
Andrew Warry ◽  
Richard D. Emes ◽  
Steven P. T. Hooton ◽  
Paul A. Barrow ◽  
...  
Keyword(s):  
Vibrio Cholerae ◽  
Genome Size ◽  
Complete Genome ◽  
Phage Therapy ◽  
Genome Sequences ◽  
Content Type ◽  
Potential Interest ◽  
Complete Genomes

ABSTRACT The complete genomes of two Vibrio cholerae bacteriophages of potential interest for cholera bacteriophage (phage) therapy were sequenced and annotated. The genome size of phage 24 is 44,395 bp encoding 71 putative proteins, and that of phage X29 is 41,569 bp encoding 68 putative proteins.

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