scholarly journals Complete Genome Sequences of Bordetella pertussis Clinical Isolate FR5810 and Reference Strain Tohama from Combined Oxford Nanopore and Illumina Sequencing

2018 ◽  
Vol 7 (19) ◽  
Author(s):  
Valérie Bouchez ◽  
Sarah Louise Baines ◽  
Sophie Guillot ◽  
Sylvain Brisse
Keyword(s):  
Clinical Isolate ◽  
Bordetella Pertussis ◽  
Complete Genome ◽  
Reference Strain ◽  
Sequence Data ◽  
Hybrid Approach ◽  
Genome Sequences ◽  
Content Type ◽  
Oxford Nanopore ◽  
Oxford Nanopore Technologies

Here, we describe the complete genome sequences of two Bordetella pertussis strains, FR5810, a clinical isolate recovered from the respiratory tract of an infant, and Tohama, a key reference strain for the species. Sequences were obtained using a hybrid approach combining Oxford Nanopore Technologies MinION and Illumina NextSeq 500 sequence data.

scholarly journals Complete Genome Sequences for Pseudomonas sp. Strains 29A and 43A

2020 ◽  
Vol 9 (39) ◽  
Author(s):  
David A. Baltrus ◽  
Meara Clark ◽  
Parris T. Humphrey ◽  
Noah K. Whiteman
Keyword(s):  
Complete Genome ◽  
Hybrid Approach ◽  
Flow Cell ◽  
Pseudomonas Sp ◽  
Genome Sequences ◽  
Content Type ◽  
Oxford Nanopore ◽  
Cardamine Cordifolia

ABSTRACT Pseudomonas sp. strains 29A and 43A were originally isolated from the phyllosphere of individual plants of Cardamine cordifolia (Brassicaceae). Here, we report complete genome sequences for these two closely related strains, assembled using a hybrid approach combining Illumina paired-end reads and longer reads sequenced on an Oxford Nanopore MinION flow cell.

scholarly journals Closed Genome Sequences of Clinical Neisseria gonorrhoeae Strains Obtained from Combined Oxford Nanopore and Illumina Sequencing

2019 ◽  
Vol 8 (9) ◽  
Author(s):  
Richard C. White ◽  
Manolito Torralba ◽  
Kelly Colt ◽  
Frank Harrison ◽  
Karrie Goglin ◽  
...  
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Hybrid Approach ◽  
Illumina Miseq ◽  
The United States ◽  
Sequencing Data ◽  
Notifiable Disease ◽  
Genome Sequences ◽  
Content Type ◽  
Oxford Nanopore ◽  
Oxford Nanopore Technologies

Neisseria gonorrhoeae is the etiological agent of gonorrhea, the second most common notifiable disease in the United States. Here, we used a hybrid approach combining Oxford Nanopore Technologies MinION and Illumina MiSeq sequencing data to obtain closed genome sequences of nine clinical N. gonorrhoeae isolates.

scholarly journals Complete Genome Sequences of Four Putatively Antibiotic-Producing Bacteria Isolated from Soil in Arkansas, USA

2022 ◽  
Author(s):  
Ruth Plymale ◽  
Griffin Hopkins ◽  
Taylor Johnson ◽  
Taylor Savage ◽  
Danielle Schaal
Keyword(s):  
Complete Genome ◽  
Soil Bacteria ◽  
Hybrid Approach ◽  
Antimicrobial Compounds ◽  
Research Experience ◽  
Genome Sequences ◽  
Valuable Source ◽  
Oxford Nanopore ◽  
Complete Genomes ◽  
Oxford Nanopore Technologies

Soil bacteria can be a valuable source of antimicrobial compounds. Here, we report the complete genomes of four soil bacteria that were isolated by undergraduate microbiology students as part of a course-based research experience. These genomes were assembled using a hybrid approach combining paired-end Illumina reads with Oxford Nanopore Technologies MinION reads.

scholarly journals Complete Circular Genome Sequences of Three Bacillus cereus Group Strains Isolated from Positive Blood Cultures from Preterm and Immunocompromised Infants Hospitalized in France

2021 ◽  
Vol 10 (41) ◽  
Author(s):  
Mariem Ben Khedher ◽  
Fredrik Nindo ◽  
Alicia Chevalier ◽  
Stéphane Bonacorsi ◽  
Gregory Dubourg ◽  
...  
Keyword(s):  
Intensive Care ◽  
Bacillus Cereus ◽  
Complete Genome ◽  
Blood Cultures ◽  
Genome Sequences ◽  
Bacillus Cereus Group ◽  
Illumina Hiseq ◽  
Content Type ◽  
Long Reads ◽  
Oxford Nanopore

We report here the complete genome sequences of three Bacillus cereus group strains isolated from blood cultures from premature and immunocompromised infants hospitalized in intensive care units in three French hospitals. These complete genome sequences were obtained from a combination of Illumina HiSeq X Ten short reads and Oxford Nanopore MinION long reads.

scholarly journals Complete Genome Sequence of the Type Strain Pectobacterium punjabense SS95, Isolated from a Potato Plant with Blackleg Symptoms

2020 ◽  
Vol 9 (32) ◽  
Author(s):  
Sohaib Sarfraz ◽  
Saïd Oulghazi ◽  
Jérémy Cigna ◽  
Shahbaz Talib Sahi ◽  
Kashif Riaz ◽  
...  
Keyword(s):  
Complete Genome ◽  
Type Strain ◽  
Gc Content ◽  
Illumina Miseq ◽  
Blackleg Disease ◽  
Circular Chromosome ◽  
Content Type ◽  
Potato Plants ◽  
Oxford Nanopore ◽  
Oxford Nanopore Technologies

ABSTRACT Pectobacterium punjabense is a newly described species causing blackleg disease in potato plants. Therefore, by the combination of long (Oxford Nanopore Technologies, MinION) and short (Illumina MiSeq) reads, we sequenced the complete genome of P. punjabense SS95T, which contains a circular chromosome of 4.793 Mb with a GC content of 50.7%.

scholarly journals Complete Genome Sequences of Pseudomonas alkylphenolica Neo and Variovorax sp. Strain CSUSB, Obtained in Undergraduate Microbiology Courses Using a Hybrid Assembly Approach

2020 ◽  
Vol 9 (9) ◽  
Author(s):  
Christopher Ne Ville ◽  
Dylan Enright ◽  
Ivan Hernandez ◽  
Jeremey Dodsworth ◽  
Paul Orwin
Keyword(s):  
Complete Genome ◽  
Hybrid Approach ◽  
Gram Negative Bacteria ◽  
Hybrid Assembly ◽  
Genome Sequences ◽  
Gram Negative ◽  
Content Type ◽  
Pesticide Tolerance

Two Gram-negative bacteria with a high G+C content were isolated from soil in undergraduate microbiology classes by enriching for low nutrient growth and neonicotinoid pesticide tolerance. DNA from these isolates was purified and sequenced using a hybrid approach. Here we report the genome sequences of Pseudomonas alkylphenolica strain Neo and Variovorax sp. strain CSUSB.

scholarly journals Complete Genome Sequence of Pasteurella multocida HuN001, a Capsular Type A Strain from a Human

2021 ◽  
Vol 10 (26) ◽  
Author(s):  
Lin Lin ◽  
Chunhui Li ◽  
Fei Wang ◽  
Xueying Wang ◽  
Yue Zhang ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Pasteurella Multocida ◽  
Sputum Sample ◽  
Capsular Type ◽  
Circular Chromosome ◽  
Content Type ◽  
Oxford Nanopore ◽  
Oxford Nanopore Technologies

Here, we report the complete genome sequence of clinical Pasteurella multocida strain HuN001, which was cultured from a sputum sample from a patient with pneumonia. Oxford Nanopore Technologies sequencing provided a complete genome sequence of P. multocida HuN001, which contains a 2,287,216-bp circular chromosome with an average G+C content of 40.33%.

scholarly journals Draft Genome Sequence of Pandrug-Resistant Pseudomonas aeruginosa SPA03, Isolated from a Patient with Benign Prostatic Hyperplasia

2021 ◽  
Vol 10 (22) ◽  
Author(s):  
Chanakya Pachi Pulusu ◽  
Balaram Khamari ◽  
Manmath Lama ◽  
Arun Sai Kumar Peketi ◽  
Prakash Kumar ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Benign Prostatic Hyperplasia ◽  
Sequence Data ◽  
Draft Genome ◽  
Prostatic Hyperplasia ◽  
Illumina Hiseq ◽  
Content Type ◽  
Oxford Nanopore ◽  
Long Read ◽  
Oxford Nanopore Technologies

The draft genome of pandrug-resistant Pseudomonas aeruginosa strain SPA03, which belongs to global high-risk sequence type 357 (ST357) and was isolated from a patient with benign prostatic hyperplasia, is presented in this report. The genome assembly was generated by combining short-read Illumina HiSeq-X Ten and long-read Oxford Nanopore Technologies MinION sequence data using the Unicycler assembler.

scholarly journals Complete Genome Sequence of Campylobacter armoricus CA639, Which Carries Two Plasmids, Compiled Using Oxford Nanopore and Illumina Sequencing Technologies

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Amine M. Boukerb ◽  
Julien Schaeffer ◽  
Joëlle Serghine ◽  
Gregory Carrier ◽  
Françoise S. Le Guyader ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Illumina Sequencing ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Sequence Data ◽  
Hybrid Approach ◽  
Circular Chromosome ◽  
Sequencing Technologies ◽  
Oxford Nanopore

As determined by a hybrid approach combining Oxford Nanopore MinION and Illumina MiniSeq sequence data, Campylobacter armoricus strain CA639 harbored a circular chromosome of 1,688,169 bp with a G+C content of 28.47% and two plasmids named pCA639-1 and pCA639-2, with lengths of 51,123 and 28,139 bp, and G+C contents of 26.5% and 28.45%, respectively.

scholarly journals Complete and Circularized Genome Assemblies of the Kroppenstedtia eburnea Genus Type Strain and the Kroppenstedtia pulmonis Species Type Strain with MiSeq and MinION Sequence Data

2020 ◽  
Vol 9 (44) ◽  
Author(s):  
Christopher A. Gulvik ◽  
Dhwani Batra ◽  
Lori A. Rowe ◽  
Milli Sheth ◽  
Ben W. Humrighouse ◽  
...  
Keyword(s):  
Type Strain ◽  
Sequence Data ◽  
Gene Clusters ◽  
Illumina Miseq ◽  
Putative Gene ◽  
Content Type ◽  
Oxford Nanopore ◽  
Species Type ◽  
Genome Assemblies ◽  
Oxford Nanopore Technologies

ABSTRACT Kroppenstedtia eburnea DSM 45196T and Kroppenstedtia pulmonis W9323T are aerobic, Gram-positive, filamentous, chemoorganotrophic thermoactinomycetes. Here, we report on the complete and circular genome assemblies generated using Illumina MiSeq and Oxford Nanopore Technologies MinION reads. Putative gene clusters predicted to be involved in the production of secondary metabolites were also identified.

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