scholarly journals Chromosomal Sequence of Lactobacillus brevis Oregon-R-modENCODE Strain BDGP6, a Lactic Acid Bacterium Isolated from the Gut of Drosophila melanogaster

2020 ◽  
Vol 9 (44) ◽  
Author(s):  
Kenneth H. Wan ◽  
Charles Yu ◽  
Soo Park ◽  
Ann S. Hammonds ◽  
Benjamin W. Booth ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Lactic Acid ◽  
Lactic Acid Bacterium ◽  
Lactobacillus Brevis ◽  
Bacterial Chromosome ◽  
Microbial Interaction ◽  
Circular Dna ◽  
Content Type ◽  
Dna Molecule ◽  
Chromosomal Sequence

ABSTRACT Lactobacillus brevis Oregon-R-modENCODE strain BDGP6 was isolated from the gut of Drosophila melanogaster for functional host-microbial interaction studies. The bacterial chromosome is a single circular DNA molecule of 2,785,111 bp with a G+C content of 46%.

Lactobacillus yonginensis sp. nov., a lactic acid bacterium with ginsenoside converting activity isolated from Kimchi

2013 ◽  
Vol 63 (Pt_9) ◽  
pp. 3274-3279 ◽  
Author(s):  
Eun-Ji Yi ◽  
Jung-Eun Yang ◽  
Jung Min Lee ◽  
YongJin Park ◽  
Sang-Yong Park ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacterium ◽  
Type Species ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
Lactobacillus Brevis ◽  
Fermented Food ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type

A Gram-reaction-positive, non-motile, non-spore-forming, catalase-negative, facultatively anaerobic, rod-shaped, β-glucosidase-producing lactic acid bacterium, designated strain THK-V8T, was isolated from the Korean fermented food, Kimchi, and its taxonomic position was investigated by using a polyphasic approach. Strain THK-V8T was able to grow at 4–40 °C (optimum, 30 °C) and pH 4.0–7.0 (optimum, pH 6.0). Strain THK-V8T had the ability to transform ginsenoside Rb1 to Rd. On the basis of 16S rRNA gene sequence similarity data, strain THK-V8T was shown to belong to the genus Lactobacillus . Strain THK-V8T was related to Lactobacillus koreensis DCY50T (98.8 % sequence similarity), Lactobacillus parabrevis LMG 11984T (97.7 %), Lactobacillus senmaizukei L13T (97.5 %), Lactobacillus hammesii TMW1.1236T (97.3 %) and Lactobacillus brevis ATCC 14687T (97.2 %). Subsequently, sequence analysis of the RNA polymerase alpha subunit gene (rpoA) confirmed that strain THK-V8T showed a maximum rpoA gene sequence similarity value of 93 % with Lactobacillus brevis LMG 6906T. The G+C content of the genomic DNA was 47.8 mol%. The DNA–DNA hybridization values between strain THK-V8T and Lactobacillus parabrevis DCY50T and Lactobacillus parabrevis LMG 11984T were 46.1±4.9 % and 10.6±2.9 %, respectively. The major fatty acids were summed feature 7 (comprised of C19 : 0 cyclo ω10c/19ω6), C14 : 0, C16 : 0 and C18 : 1ω9c. The cell wall peptidoglycan was of the A4α l-Lys–d-Asp type. The phenotypic and molecular properties indicated that strain THK-V8T represents a novel species within the genus Lactobacillus , for which the name Lactobacillus yonginensis sp. nov. is proposed. The type strain is THK-V8T ( = KACC 16236T = JCM 18023T).

scholarly journals Genome Sequence of Leuconostoc citreum DmW_111, Isolated from Wild Drosophila

2017 ◽  
Vol 5 (24) ◽  
Author(s):  
Sage M. Wright ◽  
Courtney Carroll ◽  
Amber Walters ◽  
Peter D. Newell ◽  
John M. Chaston
Keyword(s):  
Drosophila Melanogaster ◽  
Content Analysis ◽  
Lactic Acid ◽  
Lactic Acid Bacterium ◽  
Genome Sequence ◽  
Major Part ◽  
Genome Sequences ◽  
Content Type ◽  
Fermentation Processes ◽  
Leuconostoc Citreum

ABSTRACT Isolates of the lactic acid bacterium Leuconostoc citreum are a major part of fermentation processes, especially in Korean kimchi. Here, we present the genome of L. citreum DmW_111, isolated from wild Drosophila melanogaster; analysis of this genome will expand the diversity of genome sequences for non-Lactobacillus spp. isolated from D. melanogaster.

scholarly journals Complete Genome Sequence of Enterococcus durans Oregon-R-modENCODE Strain BDGP3, a Lactic Acid Bacterium Found in the Drosophila melanogaster Gut

2017 ◽  
Vol 5 (40) ◽  
Author(s):  
Kenneth H. Wan ◽  
Charles Yu ◽  
Soo Park ◽  
Ann S. Hammonds ◽  
Benjamin W. Booth ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Lactic Acid ◽  
Lactic Acid Bacterium ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Content Type ◽  
Enterococcus Durans ◽  
Interaction Studies ◽  
Single Plasmid

ABSTRACT Enterococcus durans Oregon-R-modENCODE strain BDGP3 was isolated from the Drosophila melanogaster gut for functional host-microbe interaction studies. The complete genome is composed of a single circular genome of 2,983,334 bp, with a G+C content of 38%, and a single plasmid of 5,594 bp.

scholarly journals Draft Genome Sequence of Enterococcus faecalis DD14, a Bacteriocinogenic Lactic Acid Bacterium with Anti-Clostridium Activity

2017 ◽  
Vol 5 (30) ◽  
Author(s):  
Yanath Belguesmia ◽  
Valérie Leclère ◽  
Matthieu Duban ◽  
Eric Auclair ◽  
Djamel Drider
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacterium ◽  
Enterococcus Faecalis ◽  
Genome Sequence ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Coding Sequences ◽  
Content Type ◽  
Gram Positive Bacteria ◽  
Healthy Newborn

ABSTRACT We report the draft genome sequence of Enterococcus faecalis DD14, a strain isolated from meconium of a healthy newborn at Roubaix Hospital (France). The strain displayed antagonism against a set of Gram-positive bacteria through concomitant production of lactic acid and bacteriocin. The genome has a size of 2,893,365 bp and a 37.3% G+C ratio and is predicted to contain at least 2,755 coding sequences and 62 RNAs.

scholarly journals Analysis of Transcriptomic Response to SO 2 by Oenococcus oeni Growing in Continuous Culture

2021 ◽  
Author(s):  
Cristobal A. Onetto ◽  
Peter J. Costello ◽  
Radka Kolouchova ◽  
Charlotte Jordans ◽  
Jane McCarthy ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacterium ◽  
High Sensitivity ◽  
Malolactic Fermentation ◽  
Oenococcus Oeni ◽  
Low Ph ◽  
Transcriptomic Response ◽  
Content Type ◽  
Bacterium Species ◽  
High Ethanol

Malolactic fermentation is an indispensable step in the elaboration of most wines and is generally performed by Oenococcus oeni , a Gram-positive heterofermentative lactic acid bacterium species. While O. oeni is tolerant to many of the wine stresses, including low pH and high ethanol concentrations, it has high sensitivity to SO 2 , an antiseptic and antioxidant compound regularly used in winemaking.

scholarly journals Draft Genome Sequences of 12 Leuconostoc carnosum Strains Isolated from Cooked Ham Packaged in a Modified Atmosphere and from Fresh Sausages

2020 ◽  
Vol 9 (2) ◽  
Author(s):  
Francesco Candeliere ◽  
Stefano Raimondi ◽  
Gloria Spampinato ◽  
Moon Yue Feng Tay ◽  
Alberto Amaretti ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacterium ◽  
Draft Genome ◽  
Modified Atmosphere ◽  
Genome Sequences ◽  
Content Type ◽  
Cooked Ham ◽  
Leuconostoc Carnosum ◽  
Bacteriocin Genes

Leuconostoc carnosum is a lactic acid bacterium that preferentially colonizes meat. In this work, we present the draft genome sequences of 12 Leuconostoc carnosum strains isolated from modified-atmosphere-packaged cooked ham and fresh sausages. Three strains harbor bacteriocin genes.

scholarly journals GlnR Negatively Regulates Glutamate-Dependent Acid Resistance in Lactobacillus brevis

2020 ◽  
Vol 86 (7) ◽  
Author(s):  
Luchan Gong ◽  
Cong Ren ◽  
Yan Xu
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Glutamate Decarboxylase ◽  
Wild Type Strain ◽  
Acid Resistance ◽  
Lactobacillus Brevis ◽  
Deletion Strain ◽  
Content Type ◽  
Gaba Production ◽  
Encoding Gene

ABSTRACT Lactic acid bacteria often encounter a variety of multiple stresses in their natural and industrial fermentation environments. The glutamate decarboxylase (GAD) system is one of the most important acid resistance systems in lactic acid bacteria. In this study, we demonstrated that GlnR, a nitrogen regulator in Gram-positive bacteria, directly modulated γ-aminobutyric acid (GABA) conversion from glutamate and was involved in glutamate-dependent acid resistance in Lactobacillus brevis. The glnR deletion strain (ΔglnR mutant) achieved a titer of 284.7 g/liter GABA, which is 9.8-fold higher than that of the wild-type strain. The cell survival of the glnR deletion strain was significantly higher than that of the wild-type strain under the condition of acid challenge and was positively correlated with initial glutamate concentration and GABA production. Quantitative reverse transcription-PCR assays demonstrated that GlnR inhibited the transcription of the glutamate decarboxylase-encoding gene (gadB), glutamate/GABA antiporter-encoding gene (gadC), glutamine synthetase-encoding gene (glnA), and specific transcriptional regulator-encoding gene (gadR) involved in gadCB operon regulation. Moreover, GABA production and glutamate-dependent acid resistance were absolutely abolished in the gadR glnR deletion strain. Electrophoretic mobility shift and DNase I footprinting assays revealed that GlnR directly bound to the 5′-untranslated regions of the gadR gene and gadCB operon, thus inhibiting their transcription. These results revealed a novel regulatory mechanism of GlnR on glutamate-dependent acid resistance in Lactobacillus. IMPORTANCE Free-living lactic acid bacteria often encounter acid stresses because of their organic acid-producing features. Several acid resistance mechanisms, such as the glutamate decarboxylase system, F1Fo-ATPase proton pump, and alkali production, are usually employed to relieve growth inhibition caused by acids. The glutamate decarboxylase system is vital for GAD-containing lactic acid bacteria to protect cells from DNA damage, enzyme inactivation, and product yield loss in acidic habitats. In this study, we found that a MerR-type regulator, GlnR, was involved in glutamate-dependent acid resistance by directly regulating the transcription of the gadR gene and gadCB operon, resulting in an inhibition of GABA conversion from glutamate in L. brevis. This study represents a novel mechanism for GlnR's regulation of glutamate-dependent acid resistance and also provides a simple and novel strategy to engineer Lactobacillus strains to elevate their acid resistance as well as GABA conversion from glutamate.

scholarly journals The Antisense RNA Approach: a New Application forIn VivoInvestigation of the Stress Response of Oenococcus oeni, a Wine-Associated Lactic Acid Bacterium

2015 ◽  
Vol 82 (1) ◽  
pp. 18-26 ◽  
Author(s):  
Maud Darsonval ◽  
Tarek Msadek ◽  
Hervé Alexandre ◽  
Cosette Grandvalet
Keyword(s):  
Gene Expression ◽  
Lactic Acid ◽  
Stress Response ◽  
Lactic Acid Bacterium ◽  
Antisense Rna ◽  
Expression Vector ◽  
Stress Proteins ◽  
Oenococcus Oeni ◽  
Content Type

ABSTRACTOenococcus oeniis a wine-associated lactic acid bacterium mostly responsible for malolactic fermentation in wine. In wine,O. oenigrows in an environment hostile to bacterial growth (low pH, low temperature, and ethanol) that induces stress response mechanisms. To survive,O. oeniis known to set up transitional stress response mechanisms through the synthesis of heat stress proteins (HSPs) encoded by thehspgenes, notably a unique small HSP named Lo18. Despite the availability of the genome sequence, characterization ofO. oenigenes is limited, and little is known about thein vivorole of Lo18. Due to the lack of genetic tools forO. oeni, an efficient expression vector inO. oeniis still lacking, and deletion or inactivation of thehsp18gene is not presently practicable. As an alternative approach, with the goal of understanding the biological function of theO. oenihsp18genein vivo, we have developed an expression vector to produce antisense RNA targeting ofhsp18mRNA. Recombinant strains were exposed to multiple stresses inducinghsp18gene expression: heat shock and acid shock. We showed that antisense attenuation ofhsp18affectsO. oenisurvival under stress conditions. These results confirm the involvement of Lo18 in heat and acid tolerance ofO. oeni. Results of anisotropy experiments also confirm a membrane-protective role for Lo18, as previous observations had already suggested. This study describes a new, efficient tool to demonstrate the use of antisense technology for modulating gene expression inO. oeni.

scholarly journals Arabinoxylan Oligosaccharide Hydrolysis by Family 43 and 51 Glycosidases from Lactobacillus brevis DSM 20054

2013 ◽  
Vol 79 (21) ◽  
pp. 6747-6754 ◽  
Author(s):  
Herbert Michlmayr ◽  
Johannes Hell ◽  
Cindy Lorenz ◽  
Stefan Böhmdorfer ◽  
Thomas Rosenau ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Functional Food ◽  
Specific Activity ◽  
High Specific Activity ◽  
Lactobacillus Brevis ◽  
Glycoside Hydrolases ◽  
Content Type ◽  
Food And Feed ◽  
Oligosaccharide Hydrolysis

ABSTRACTDue to their potential prebiotic properties, arabinoxylan-derived oligosaccharides [(A)XOS] are of great interest as functional food and feed ingredients. While the (A)XOS metabolism ofBifidobacteriaceaehas been extensively studied, information regarding lactic acid bacteria (LAB) is still limited in this context. The aim of the present study was to fill this important gap by characterizing candidate (A)XOS hydrolyzing glycoside hydrolases (GHs) identified in the genome ofLactobacillus brevisDSM 20054. Two putative GH family 43 xylosidases (XynB1 and XynB2) and a GH family 43 arabinofuranosidase (Abf3) were heterologously expressed and characterized. While the function of XynB1 remains unclear, XynB2 could efficiently hydrolyze xylooligosaccharides. Abf3 displayed high specific activity for arabinobiose but could not release arabinose from an (A)XOS preparation. However, two previously reported GH 51 arabinofuranosidases fromLb. breviswere able to specifically remove α-1,3-linked arabinofuranosyl residues from arabino-xylooligosaccharides (AXHm3 specificity). These results imply thatLb. brevisis at least genetically equipped with functional enzymes in order to hydrolyze the depolymerization products of (arabino)xylans and arabinans. The distribution of related genes inLactobacillalesgenomes indicates that GH 43 and, especially, GH 51 glycosidase genes are rare among LAB and mainly occur in obligately heterofermentativeLactobacillusspp.,Pediococcusspp., members of theLeuconostoc/Weissellabranch, andEnterococcusspp. Apart from the prebiotic viewpoint, this information also adds new perspectives on the carbohydrate (i.e., pentose-oligomer) metabolism of LAB species involved in the fermentation of hemicellulose-containing substrates.

Sign in / Sign up

Export Citation Format

Share Document