The study of the bioenergetic characteristics of the red blood cells of Black Sea fish: the common stingray (Dasyatis pastinaca L.) and black scorpionfish (Scorpaena porcus L.)

BIOPHYSICS ◽  
2017 ◽  
Vol 62 (3) ◽  
pp. 434-439 ◽  
Author(s):  
Yu. A. Silkin ◽  
S. M. Korotkov ◽  
E. N. Silkina
Keyword(s):  
Red Blood Cells ◽  
Black Sea ◽  
Blood Cells ◽  
The Common ◽  
Sea Fish ◽  
Scorpaena Porcus

Effect of proteinate or sulphate mineral sources on trace elements in blood and liver of piglets

2000 ◽  
Vol 71 (1) ◽  
pp. 131-139 ◽  
Author(s):  
S. Schiavon ◽  
L. Bailoni ◽  
M. Ramanzin ◽  
R. Vincenzi ◽  
A. Simonetto ◽  
...  
Keyword(s):  
Trace Elements ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Plasma Levels ◽  
Natural Food ◽  
Blood Samples ◽  
Mineral Supplements ◽  
The Common ◽  
Cu And Zn

AbstractFour hundred piglets were housed in 20 pens and offered for 42 days a pre-starter and then a starter compound supplemented with trace elements given as sulphates (SULF) or proteinates (PROT) at a common level (100) or at a reduced level (20) of inclusion. The common level supplied 278, 148, 315 and 98 mg/kg and the reduced level supplied 128, 38, 135 and 50 mg/kg of iron (Fe), copper (Cu), zinc (Zn) and manganese (Mn), respectively, taking into account the natural food contents. Proteinates used in the trial were analysed and described in terms of content and quality of different potential ligands. Piglet growth was not affected by any treatment. At the end of the trial blood samples were collected from eight pigs for each treatment. These animals were slaughtered and their livers were removed, weighed and analysed. Compared with SULF, PROT increased significantly plasma levels of Fe (25·1 v. 15·7 μmol/l), haemoglobin (10·9 v. 10·4 g/dl) and the number of red blood cells (6·4 v. 6·1 millions per μl) but the liver recovery of Fe was not affected by any treatment. In piglets receiving PROT the liver content of Cu and Zn increased significantly compared with those receiving SULF. On reducing the dosage, Cu in the liver significantly decreased with SULF but not with PROT and the amount of Zn decreased more with SULF than with PROT. The results may reflect a better availability of Cu and Zn when proteinates rather than sulphates were used as mineral supplements.

Rapid renewal of red blood cells in the common carp following prolonged exposure to air

2014 ◽  
Vol 81 (2) ◽  
pp. 255-265 ◽  
Author(s):  
Yen-Hua Chen ◽  
Hong-Han Chen ◽  
Sen-Shyong Jeng
Keyword(s):  
Red Blood Cells ◽  
Common Carp ◽  
Blood Cells ◽  
Prolonged Exposure ◽  
The Common

scholarly journals Comparative characteristics of the ultrastructure of nephron cells in some species of pelagic, epibenthic, and demersal fish (the Karantinnaya Bay, the Black Sea)

2021 ◽  
Vol 6 (2) ◽  
pp. 95-109
Author(s):  
E. A. Flerova ◽  
G. M. Chuyko
Keyword(s):  
Epithelial Cells ◽  
Black Sea ◽  
Demersal Fish ◽  
Tissue Level ◽  
The Black Sea ◽  
Type I ◽  
Single Structure ◽  
Different Depths ◽  
Sea Fish ◽  
Scorpaena Porcus

The ultrastructure of the main sections of the mesonephros nephron in Black Sea teleost fish is studied. The species investigated are as follows: pelagic Trachurus mediterraneus (Steindachner, 1868) and Chelon auratus (Risso, 1810); epibenthic Diplodus annularis (Linnaeus, 1758) and Spicara flexuosa Rafinesque, 1810; and demersal Scorpaena porcus Linnaeus, 1758, Gobius niger Linnaeus, 1758, and Mullus barbatus ponticus Essipov, 1927. It is shown that in Black Sea fish, which inhabit different depths and are under different conditions of environmental osmotic pressure, nephrons at the tissue level of organization have a single structure and form glomerular kidneys. Fish adaptability to the habitat at certain depths is primarily manifested in an increase in the number and size of mitochondria of all types of nephron epithelial cells. A decrease in the renal corpuscles area, the length of podocytes, and height of tubular epithelial cells, as well as the brush border length of type I proximal tubules is also recorded. Nephron cytological peculiarities of pelagic, epibenthic, and demersal fish characterize a high adaptive capacity of the mesonephros cellular structures.

scholarly journals Erythrocytes of circulating blood of scorpionfish Scorpaena porcus L. 1758 under acute experimental hypoxia

2018 ◽  
Vol 3 (4) ◽  
pp. 92-100
Author(s):  
A. A. Soldatov ◽  
T. A. Kukhareva ◽  
A. Yu. Andreeva ◽  
I. A. Parfenova
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Hematopoietic Tissue ◽  
Short Term ◽  
Saturation Control ◽  
Oxygen Saturation Level ◽  
Oxygen Capacity ◽  
Abnormal Cells ◽  
Blood Characteristics ◽  
Scorpaena Porcus

The effect of short-term hypoxia on the black scorpionfish (Scorpaena porcus L.) red blood cells has been investigated. Fish were exposed to 8.5–8.7 mg O2·l-1 (100 % saturation, control), 2.6 mg O2·l-1 (30 % saturation) and 1.3 mg O2·l-1 (15 % saturation) for 90 minutes. At 30 % saturation all parameters were kept at the control values. At oxygen-saturation level of 15 % we observed inhibition of erythropoietic processes in hematopoietic tissue, as number of immature erythroid forms (basophilic normoblasts) in the blood has reduced. This process was accompanied with circulating red blood cells swelling and lysis of osmotically fragile erythocytes and abnormal cells. Thus, blood oxygen capacity declined, and osmotic resistance of red blood cells increased. It is supposed, that such changes of blood characteristics should induce the production of erythropoietin in fish kidneys and also enhance proliferation of erythroid cells in hematopoietic tissue.

scholarly journals (More than) doubling down: Effective fibrinolysis at a reduced rt-PA dose for catheter-directed thrombolysis combined with histotripsy

PLoS ONE ◽  
2022 ◽  
Vol 17 (1) ◽  
pp. e0261567
Author(s):  
Samuel A. Hendley ◽  
Aarushi Bhargava ◽  
Christy K. Holland ◽  
Geoffrey D. Wool ◽  
Osman Ahmed ◽  
...  
Keyword(s):  
Deep Vein Thrombosis ◽  
Thrombolytic Therapy ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Vein Thrombosis ◽  
Clinical Dose ◽  
Infusion Catheter ◽  
Catheter Directed Thrombolysis ◽  
The Common ◽  
Deep Vein

Deep vein thrombosis is a major source of morbidity and mortality worldwide. For acute proximal deep vein thrombosis, catheter-directed thrombolytic therapy is an accepted method for vessel recanalization. Thrombolytic therapy is not without risk, including the potential for hemorrhagic bleeding that increases with lytic dose. Histotripsy is a focused ultrasound therapy that generates bubble clouds spontaneously in tissue at depth. The mechanical activity of histotripsy increases the efficacy of thrombolytic therapy at doses consistent with current pharmacomechanical treatments for venous thrombosis. The objective of this study was to determine the influence of lytic dose on histotripsy-enhanced fibrinolysis. Human whole blood clots formed in vitro were exposed to histotripsy and a thrombolytic agent (recombinant tissue plasminogen activator, rt-PA) in a venous flow model perfused with plasma. Lytic was administered into the clot via an infusion catheter at concentrations ranging from 0 (control) to 4.54 μg/mL (a common clinical dose for catheter-directed thrombolysis). Following treatment, perfusate samples were assayed for markers of fibrinolysis, hemolysis, and intact red blood cells and platelets. Fibrinolysis was equivalent between the common clinical dose of rt-PA (4.54 μg/mL) and rt-PA at a reduction to one-twentieth of the common clinical dose (0.23 μg/mL) when combined with histotripsy. Minimal changes were observed in hemolysis for treatment arms with or without histotripsy, potentially due to clot damage from insertion of the infusion catheter. Likewise, histotripsy did not increase the concentration of red blood cells or platelets in the perfusate following treatment compared to rt-PA alone. At the highest lytic dose, a refined histotripsy exposure scheme was implemented to cover larger areas of the clot. The updated exposure scheme improved clot mass loss and fibrinolysis relative to administration of lytic alone. Overall, the data collected in this study indicate the rt-PA dose can be reduced by more than a factor of ten and still promote fibrinolysis when combined with histotripsy.

Physiological and behaviour changes in common lizards parasitized by haemogregarines

Parasitology ◽  
1996 ◽  
Vol 113 (5) ◽  
pp. 433-438 ◽  
Author(s):  
A. Oppliger ◽  
M. L. Célérier ◽  
J. Clobert
Keyword(s):  
Oxygen Consumption ◽  
Body Temperature ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Haemoglobin Concentration ◽  
Reproductive Costs ◽  
Multiplication Rate ◽  
Common Lizard ◽  
The Common ◽  
Behaviour Changes

SUMMARYThe effect of haemoparasites on the physiology and behaviour traits of their hosts was examined using Haemogregarina sp., a parasite of the common lizard, Lacerta vivipara, from the south of France. Infection with haemogregarines was associated with a reduced haemoglobin concentration and an increased number of immature red blood cells. Parasitized individuals also showed a reduced oxygen consumption at rest and a lower locomotor speed. We also found that the multiplication rate of the parasite depended on the temperature at which the lizard was maintained. Between 21 and 28 °C the multiplication rate of the parasite was significantly lower than between 29 and 35 °C. This suggests that the parasites may suffer reproductive costs when hosts reduce their body temperature.

scholarly journals Phototheranostics Using Erythrocyte-Based Particles

Biomolecules ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 729
Author(s):  
Taylor Hanley ◽  
Raviraj Vankayala ◽  
Chi-Hua Lee ◽  
Jack C. Tang ◽  
Joshua M. Burns ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Immune Evasion ◽  
Blood Cells ◽  
Delivery Systems ◽  
Clinical Translation ◽  
Exciting Field ◽  
Future Directions ◽  
Recent Increase ◽  
The Common ◽  
Insight Into

There has been a recent increase in the development of delivery systems based on red blood cells (RBCs) for light-mediated imaging and therapeutic applications. These constructs are able to take advantage of the immune evasion properties of the RBC, while the addition of an optical cargo allows the particles to be activated by light for a number of promising applications. Here, we review some of the common fabrication methods to engineer these constructs. We also present some of the current light-based applications with potential for clinical translation, and offer some insight into future directions in this exciting field.

Hemoglobin crystals of the red blood cells in the human renal cortex: electron microscopic observations of the renal lithiasis

1978 ◽  
Vol 36 (2) ◽  
pp. 480-481
Author(s):  
Kosuke Ueda ◽  
Hiroto Washida ◽  
Nakazo Watari
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Renal Cortex ◽  
Uranyl Acetate ◽  
Osmic Acid ◽  
Renal Lithiasis ◽  
Electron Microscopic ◽  
Hemoglobin C ◽  
First Case ◽  
Ultrathin Sections

IntroductionHemoglobin crystals in the red blood cells were electronmicroscopically reported by Fawcett in the cat myocardium. In the human, Lessin revealed crystal-containing cells in the periphral blood of hemoglobin C disease patients. We found the hemoglobin crystals and its agglutination in the erythrocytes in the renal cortex of the human renal lithiasis, and these patients had no hematological abnormalities or other diseases out of the renal lithiasis. Hemoglobin crystals in the human erythrocytes were confirmed to be the first case in the kidney.Material and MethodsTen cases of the human renal biopsies were performed on the operations of the seven pyelolithotomies and three ureterolithotomies. The each specimens were primarily fixed in cacodylate buffered 3. 0% glutaraldehyde and post fixed in osmic acid, dehydrated in graded concentrations of ethanol, and then embedded in Epon 812. Ultrathin sections, cut on LKB microtome, were doubly stained with uranyl acetate and lead citrate.

Densitometric Identification of Primitive Erythroblasts After Reaction With Diaminobenzidine

1973 ◽  
Vol 31 ◽  
pp. 328-329
Author(s):  
John A. Trotter
Keyword(s):  
Red Blood Cells ◽  
Analytical Method ◽  
Blood Cells ◽  
Guinea Pigs ◽  
Specific Protein ◽  
Visual Examination ◽  
Hemoglobin Synthesis ◽  
Peroxidatic Activity ◽  
Small Density

Hemoglobin is the specific protein of red blood cells. Those cells in which hemoglobin synthesis is initiated are the earliest cells that can presently be considered to be committed to erythropoiesis. In order to identify such early cells electron microscopically, we have made use of the peroxidatic activity of hemoglobin by reacting the marrow of erythropoietically stimulated guinea pigs with diaminobenzidine (DAB). The reaction product appeared as a diffuse and amorphous electron opacity throughout the cytoplasm of reactive cells. The detection of small density increases of such a diffuse nature required an analytical method more sensitive and reliable than the visual examination of micrographs. A procedure was therefore devised for the evaluation of micrographs (negatives) with a densitometer (Weston Photographic Analyzer).

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