Identification of Pathways of A498 Human Kidney Carcinoma Cell Death under the Action of Gratiola officinalis L. Extract and Green Tea Flavonoids Using Fluorescence Imaging Techniques

Cell death occurring in embryoid bodies derived from the embryonal carcinoma cell line, PSA4, which undergo cavitation, and in those from the related cell line S2, which do not undergo cavitation, was classified as apoptosis or necrosis by ultrastructural criteria. Both modes of cell death were seen in PSA4 embryoid bodies while apoptosis alone was seen in S2 embryoid bodies. No significant difference was found between PSA4 and S2 embryoid bodies either in apoptotic incidence score or in the spatial distribution of apoptotic events. We therefore conclude that although apoptosis and tissue modelling coexist in PSA4 embryoid bodies, necrosis rather than apoptosis is causally related to formation of the cavity.

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Knockdown of β-catenin controls both apoptotic and autophagic cell death through LKB1/AMPK signaling in head and neck squamous cell carcinoma cell lines

Cellular Signalling ◽

10.1016/j.cellsig.2012.12.020 ◽

2013 ◽

Vol 25 (4) ◽

pp. 839-847 ◽

Cited By ~ 36

Author(s):

Hyo Won Chang ◽

Yoon Se Lee ◽

Hae Yun Nam ◽

Myoung Wol Han ◽

Hyo Jung Kim ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Cell Death ◽

Cell Carcinoma ◽

Head And Neck ◽

Cell Lines ◽

Squamous Cell ◽

Carcinoma Cell ◽

Squamous Cell Carcinoma Cell ◽

Ampk Signaling ◽

Carcinoma Cell Lines

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Fluorescence imaging with tailored light

Nanophotonics ◽

10.1515/nanoph-2019-0227 ◽

2019 ◽

Vol 8 (12) ◽

pp. 2111-2128 ◽

Cited By ~ 3

Author(s):

Jialei Tang ◽

Jinhan Ren ◽

Kyu Young Han

Keyword(s):

Fluorescence Imaging ◽

Imaging Techniques ◽

Super Resolution ◽

Optical Parameters ◽

Propagation Angle ◽

Resolution Imaging ◽

Time Observation ◽

Living Organisms ◽

Real Time Observation

AbstractFluorescence microscopy has long been a valuable tool for biological and medical imaging. Control of optical parameters such as the amplitude, phase, polarization, and propagation angle of light gives fluorescence imaging great capabilities ranging from super-resolution imaging to long-term real-time observation of living organisms. In this review, we discuss current fluorescence imaging techniques in terms of the use of tailored or structured light for the sample illumination and fluorescence detection, providing a clear overview of their working principles and capabilities.

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Tumor cell death visualization of renal cell carcinoma under the combined effect of the Gratiola officinalis extract and cyclophosphamide using fluorescent staining methods

Journal of Innovative Optical Health Sciences ◽

10.1142/s1793545821420049 ◽

2021 ◽

pp. 2142004

Author(s):

Artyom Mylnikov ◽

Nikita Navolokin ◽

Dmitry Mudrak ◽

Natalya Polukonova ◽

Alla Bucharskaya ◽

...

Keyword(s):

Cell Death ◽

Tumor Cells ◽

Human Kidney ◽

Combined Effect ◽

Tumor Cell Death ◽

Staining Methods ◽

Apoptotic Activity ◽

Apoptosis And Necrosis ◽

Number Of Cells

Objective of the study: We used fluorescence imaging methods of apoptosis and necrosis in human renal carcinoma A498 tumor cells in vitro to reveal the indicated forms of cell death under the combined effect of flavonoid-containing extract of Gratiola officinalis and cytostatic (cyclophosphamide). Materials and methods: The dyes were propidium iodide and acridine orange, which were used in the “alive and dead” test. This test helped us to identify the total number of dead cells in the forms of necrosis and apoptosis and the number of cells in which apoptosis had started, it was characterized by the appearance of apoptotic bodies or nucleus pyknosis. Results: We found the most pronounced cytotoxic activity at the ratio of extract of Gratiola officinalis and cyclophosphamide concentrations of 1:1. The number of living cells decreased when exposed to the ratio of extract and cytostatic concentrations of 2:1. When the ratio of concentration of the extract relative to the cytostatic increased to 3:1, the cytostatic activity of the extract began to appear, the total number of tumor cells decreased. The number of cells with nucleus pyknosis and the number of cells with apoptosis signs significantly increased at a 3:1 ratio of extract and cytostatic concentrations, which confirms the presence of pro-apoptotic activity of the studied combination. This trend indicates the dependence of a certain form of cell death (apoptosis, necrosis) on the ratio of extract and cytostatic doses, and it also demonstrates the cytostatic and cytotoxic effects of this combination. Conclusion: Fluorescence methods of investigation in the “alive and dead” test allowed us to visualize the forms of cell death of human kidney carcinoma A498 by combined exposure to the flavonoid-containing extract of Gratiola officinalis and cytostatic (cyclophosphamide) 24 h after exposure. We found that the combination with a concentration ratio of the extract and cyclophosphamide of 3:1 has the greatest effectiveness due to stimulation of the cytostatic effect and cytotoxic effect.

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