Sucrose Synthase (SuSy) Gene Expression: An Indicator for Cotton Fiber Initiation and Early Development

2019 ◽  
Vol 66 (1) ◽  
pp. 41-49 ◽  
Author(s):  
M. Ahmed ◽  
S. Akhtar ◽  
M. Fanglu ◽  
M. M. Hasan ◽  
A. A. Shahid ◽  
...  
Plants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2226
Author(s):  
Jorge Xool-Tamayo ◽  
Yahaira Tamayo-Ordoñez ◽  
Miriam Monforte-González ◽  
José Armando Muñoz-Sánchez ◽  
Felipe Vázquez-Flota

The synthesis of the benzylisoquinoline alkaloids, sanguinarine and berberine, was monitored in Argemone mexicana L. (Papaveracea) throughout the early stages of its hypocotyl and seedling development. Sanguinarine was detected in the cotyledons right after hypocotyl emergence, and it increased continuously until the apical hook unbent, prior to the cotyledonary leaves unfolding, when it abruptly fell. In the cotyledonary leaves, it also remained at low levels. Throughout development, berberine accumulation required the formation of cotyledonary leaves, whereas it was quickly detected in the hypocotyl from the time it emerged. Interestingly, the alkaloids detected in the cotyledons could have been imported from hypocotyls, because no transcriptional activity was detected in there. However, after turning into cotyledonary leaves, important levels of gene expression were noted. Taken together, these results suggest that the patterns of alkaloid tissue distribution are established from very early development, and might require transport systems.


PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4615 ◽  
Author(s):  
Lan Jiang ◽  
Qingqing Wang ◽  
Jue Yu ◽  
Vinita Gowda ◽  
Gabriel Johnson ◽  
...  

The budgerigar (Melopsittacus undulatus) is one of the most widely studied parrot species, serving as an excellent animal model for behavior and neuroscience research. Until recently, it was unknown how sexual differences in the behavior, physiology, and development of organisms are regulated by differential gene expression. MicroRNAs (miRNAs) are endogenous short non-coding RNA molecules that can post-transcriptionally regulate gene expression and play a critical role in gonadal differentiation as well as early development of animals. However, very little is known about the role gonadal miRNAs play in the early development of birds. Research on the sex-biased expression of miRNAs in avian gonads are limited, and little is known aboutM. undulatus. In the current study, we sequenced two small non-coding RNA libraries made from the gonads of adult male and female budgerigars using Illumina paired-end sequencing technology. We obtained 254 known and 141 novel miRNAs, and randomly validated five miRNAs. Of these, three miRNAs were differentially expressed miRNAs and 18 miRNAs involved in sexual differentiation as determined by functional analysis with GO annotation and KEGG pathway analysis. In conclusion, this work is the first report of sex-biased miRNAs expression in the budgerigar, and provides additional sequences to the avian miRNAome database which will foster further functional genomic research.


Ecotoxicology ◽  
2003 ◽  
Vol 12 (6) ◽  
pp. 469-474 ◽  
Author(s):  
P. R. Hoyt ◽  
M. J. Doktycz ◽  
K. L. Beattie ◽  
M. S. Greeley

Development ◽  
1998 ◽  
Vol 125 (18) ◽  
pp. 3689-3698
Author(s):  
X. Mu ◽  
B. Lee ◽  
J.M. Louis ◽  
A.R. Kimmel

Major stages of Dictyostelium development are regulated by secreted, extracellular cAMP through activation of a serpentine receptor family. During early development, oscillations of extracellular cAMP mobilize cells for aggregation; later, continuous exposure to higher extracellular cAMP concentrations downregulates early gene expression and promotes cytodifferentiation and cell-specific gene expression. The cAMP receptor 1 gene CAR1 has two promoters that are differentially responsive to these extracellular cAMP stimuli. The early CAR1 promoter is induced by nM pulses of cAMP, which in turn are generated by CAR1-dependent activation of adenylyl cyclase (AC). Higher, non-fluctuating concentrations of cAMP will adapt this AC stimulus-response, repress the activated early promoter and induce the dormant late promoter. We now identify a critical element of the pulse-induced CAR1 promoter and a nuclear factor with sequence-specific interaction. Mutation of four nucleotides within the element prevents both in vitro protein binding and in vivo expression of an otherwise fully active early CAR1 promoter and multimerization of the wild-type, but not mutant, sequence will confer cAMP regulation to a quiescent heterologous promoter. These cis and trans elements, thus, constitute a part of the molecular response to the cAMP transmembrane signal cascade that regulates early development of Dictyostelium.


Development ◽  
1994 ◽  
Vol 1994 (Supplement) ◽  
pp. 163-168
Author(s):  
J. Richard Hinchliffe

New insights into the origin of the tetrapod limb, and its early development and patterning, are emerging from a variety of fields. A wide diversity of approaches was reported at the BSDB Spring Symposium on `The Evolution of Developmental Mechanisms' (Edinburgh, 1994); here I review the contributions these various approaches have made to understanding the evolutionary developmental biology of the tetrapod limb. The fields covered included palaeontology, descriptive embryology, experimental embryological analysis of interactions within developing limbs plus description and manipulation of homeobox gene expression in early limb buds. Concepts are equally varied, sometimes conflicting, sometimes overlapping. Some concern the limb `archetype' (can the palaeontologists and morphologists still define this with precision? how far is there a limb developmental bauplan?); others are based on identification of epigenetic factors (eg secondary inductions), as generating pattern; while yet others assume a direct gene-morphology relationship. But all the contributors ask the same compelling question: can we explain both the similarity (homology) and variety of tetrapod limbs (and the fins of the Crossopterygians) in terms of developmental mechanisms?


Sign in / Sign up

Export Citation Format

Share Document