The Physiological Basis of Genotypic Variations in Low-Oxygen Stress Tolerance in the Vegetable Sweet Potato

Waterlogging (WL), excess water in the soil, is a phenomenon often occurring during plant cultivation causing low oxygen levels (hypoxia) in the soil. The aim of this study was to identify candidate genes involved in long-term waterlogging tolerance in cucumber using RNA sequencing. Here, we also determined how waterlogging pre-treatment (priming) influenced long-term memory in WL tolerant (WL-T) and WL sensitive (WL-S) i.e., DH2 and DH4 accessions, respectively. This work uncovered various differentially expressed genes (DEGs) activated in the long-term recovery in both accessions. De novo assembly generated 36,712 transcripts with an average length of 2236 bp. The results revealed that long-term waterlogging had divergent impacts on gene expression in WL-T DH2 and WL-S DH4 cucumber accessions: after 7 days of waterlogging, more DEGs in comparison to control conditions were identified in WL-S DH4 (8927) than in WL-T DH2 (5957). Additionally, 11,619 and 5007 DEGs were identified after a second waterlogging treatment in the WL-S and WL-T accessions, respectively. We identified genes associated with WL in cucumber that were especially related to enhanced glycolysis, adventitious roots development, and amino acid metabolism. qRT-PCR assay for hypoxia marker genes i.e., alcohol dehydrogenase (adh), 1-aminocyclopropane-1-carboxylate oxidase (aco) and long chain acyl-CoA synthetase 6 (lacs6) confirmed differences in response to waterlogging stress between sensitive and tolerant cucumbers and effectiveness of priming to enhance stress tolerance.

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Response of pomegranate arils (cv. Wonderful) to low oxygen stress under active modified atmosphere condition

Journal of the Science of Food and Agriculture ◽

10.1002/jsfa.9276 ◽

2018 ◽

Vol 99 (3) ◽

pp. 1088-1097 ◽

Cited By ~ 1

Author(s):

Zinash A Belay ◽

Oluwafemi J Caleb ◽

Pramod V Mahajan ◽

Umezuruike L Opara

Keyword(s):

Modified Atmosphere ◽

Low Oxygen ◽

Oxygen Stress ◽

Pomegranate Arils

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Differential response of Daphnia genotypes to oxygen stress: respiration rates, hemoglobin content and low-oxygen tolerance

Oecologia ◽

10.1007/bf00379661 ◽

1985 ◽

Vol 65 (4) ◽

pp. 487-491 ◽

Cited By ~ 87

Author(s):

Lawrence J. Weider ◽

Winfried Lampert

Keyword(s):

Differential Response ◽

Low Oxygen ◽

Hemoglobin Content ◽

Oxygen Stress ◽

Oxygen Tolerance ◽

Respiration Rates

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Low-Oxygen Stress in Plants

10.1007/978-3-7091-1254-0 ◽

2014 ◽

Cited By ~ 7

Keyword(s):

Low Oxygen ◽

Oxygen Stress

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Pyrophosphate as an alternative energy currency in plants

Biochemical Journal ◽

10.1042/bcj20200940 ◽

2021 ◽

Vol 478 (8) ◽

pp. 1515-1524

Author(s):

Abir U. Igamberdiev ◽

Leszek A. Kleczkowski

Keyword(s):

Metabolic Pathways ◽

Energy Efficient ◽

Alternative Energy ◽

Plant Cells ◽

Atp Synthesis ◽

Continuous Operation ◽

Low Oxygen ◽

Pyruvate Phosphate Dikinase ◽

Oxygen Stress ◽

Membrane Bound

In the conditions of [Mg2+] elevation that occur, in particular, under low oxygen stress and are the consequence of the decrease in [ATP] and increase in [ADP] and [AMP], pyrophosphate (PPi) can function as an alternative energy currency in plant cells. In addition to its production by various metabolic pathways, PPi can be synthesized in the combined reactions of pyruvate, phosphate dikinase (PPDK) and pyruvate kinase (PK) by so-called PK/PPDK substrate cycle, and in the reverse reaction of membrane-bound H+-pyrophosphatase, which uses the energy of electrochemical gradients generated on tonoplast and plasma membrane. The PPi can then be consumed in its active forms of MgPPi and Mg2PPi by PPi-utilizing enzymes, which require an elevated [Mg2+]. This ensures a continuous operation of glycolysis in the conditions of suppressed ATP synthesis, keeping metabolism energy efficient and less dependent on ATP.

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Mechanisms and evolution of resistance to environmental extremes in animals

EvoDevo ◽

10.1186/s13227-019-0143-4 ◽

2019 ◽

Vol 10 (1) ◽

Cited By ~ 3

Author(s):

Thomas C. Boothby

Keyword(s):

Environmental Stress ◽

Stress Tolerance ◽

Molecular Mechanisms ◽

Low Oxygen ◽

The Third ◽

Environmental Extremes ◽

Oxygen Conditions ◽

Health Related ◽

Evolution Of Resistance ◽

Intense Heat

AbstractWhen animals are exposed to an extreme environmental stress, one of three possible outcomes takes place: the animal dies, the animal avoids the environmental stress and survives, or the animal tolerates the environmental stress and survives. This review is concerned with the third possibility, and will look at mechanisms that rare animals use to survive extreme environmental stresses including freezing, desiccation, intense heat, irradiation, and low-oxygen conditions (hypoxia). In addition, an increasing understanding of the molecular mechanisms involved in environmental stress tolerance allows us to speculate on how these tolerances arose. Uncovering the mechanisms of extreme environmental stress tolerance and how they evolve has broad implications for our understanding of the evolution of early life on this planet, colonization of new environments, and the search for novel forms of life both on Earth and elsewhere, as well as a number of agricultural and health-related applications.

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