The Time Course of Muscle Nuclear Content of Transcription Factors Regulating the MyHC I(β) Expression in the Rat Soleus Muscle under Gravitational Unloading

2020 ◽  
Vol 14 (3) ◽  
pp. 242-248
Author(s):  
I. I. Paramonova ◽  
K. A. Sharlo ◽  
N. A. Vilchinskaya ◽  
B. S. Shenkman
Keyword(s):  
Transcription Factors ◽  
Soleus Muscle ◽  
Time Course ◽  
Gravitational Unloading ◽  
Rat Soleus Muscle ◽  
Nuclear Content

Mechanism of insulin-induced activation of Na(+)-K(+)-ATPase in isolated rat soleus muscle

1991 ◽  
Vol 261 (2) ◽  
pp. C224-C230 ◽  
Author(s):  
E. Weil ◽  
S. Sasson ◽  
Y. Gutman
Keyword(s):  
Soleus Muscle ◽  
Time Course ◽  
Maximal Effect ◽  
Dependent Manner ◽  
Insulin Stimulation ◽  
K Uptake ◽  
Sequence Of Events ◽  
Rat Soleus Muscle ◽  
Isolated Rat ◽  
Stimulation Of

Insulin augments Na(+)-K(+)-ATPase activity in skeletal muscles. It has been proposed that the sequence of events is activation of Na(+)-H+ antiporter, increased intracellular Na+ concentration ( [Na+]i), and stimulation of Na(+)-K+ pump. We have used isolated rat soleus muscles to test this hypothesis. Insulin increased the ouabain-suppressible K+ uptake in a dose- and time-dependent manner. The maximal effect was observed at 50-100 mU/ml insulin. Stimulation of K+ uptake was accompanied by increased specific [3H]ouabain binding and lowered [Na+]i. The ionophore monensin, which promotes Na(+)-H+ exchange, also increased the rate of ouabain-suppressible K+ uptake in soleus muscle, with a maximal effect obtained at 10-100 microM ionophore. However, this increase was accompanied by an elevation of [Na+]i. In the presence of 10-100 microM monensin, addition of 100 mU/ml insulin further increased K+ uptake but reduced [Na+]i. The effect on K+ uptake was additive. Ouabain (10(-3) M) completely suppressed the effect of insulin on [Na+]i. Insulin had no effect on the magnitude or the time course of insulin stimulation of K+ uptake. Thus equal stimulation of Na(+)-K(+)-ATPase by insulin was observed when [Na+]i was elevated (under monensin) or lowered (under amiloride). These data suggest that activation of Na(+)-K(+)-ATPase in soleus muscle by insulin is not secondary to stimulation of Na(+)-H+ antiporter.

Time course of the T3- and T4-induced increase in rat soleus muscle mitochondria

1979 ◽  
Vol 236 (3) ◽  
pp. C132-C138 ◽  
Author(s):  
W. W. Winder
Keyword(s):  
Cytochrome C ◽  
Thyroid Hormones ◽  
Soleus Muscle ◽  
Time Course ◽  
Citrate Synthase ◽  
Creatine Phosphate ◽  
Simultaneous Treatment ◽  
Muscle Mitochondria ◽  
Rat Soleus Muscle ◽  
Motor Nerves

Citrate synthase and cytochrome c increase in soleus muscle of rats in response to excess thyroid hormones. The half times of the increase in the levels of citrate synthase and cytochrome c in soleus muscle during induction are greater than the half times of the decline in enzyme levels after cessation of treatment (15 days vs. 7 days for citrate synthase). Denervation of the soleus does not prevent the increase in citrate synthase in response to thyrotoxicosis. This provides evidence that thyroid hormones affect the muscle directly and not via the motor nerves. ATP concentration is reduced in liver, but not in soleus muscle in response to thyrotoxicosis. Creatine phosphate is not significantly altered in soleus muscle. Cyclic AMP is slightly lower in thyrotoxic soleus muscle. Simultaneous treatment with thyroid hormones and propranolol does not affect the increase in citrate synthase in response to excess thyroid hormones. It is concluded that the increase in muscle mitochondria associated with thyrotoxicosis is not mediated via the nervous system or by a cAMP-regulated process.

Effects of Deafferentation on the Size and Myosin Phenotype of Muscle Fibers on Stretching of the Rat Soleus Muscle in Conditions of Gravitational Unloading

2004 ◽  
Vol 34 (7) ◽  
pp. 755-763 ◽  
Author(s):  
T. L. Nemirovskaya ◽  
B. S. Shenkman ◽  
A. M. Mukhina ◽  
Ya. Yu. Volodkovich ◽  
M. M. Sayapina ◽  
...  
Keyword(s):  
Soleus Muscle ◽  
Muscle Fibers ◽  
Gravitational Unloading ◽  
Rat Soleus Muscle

Glycogen supercompensation in rat soleus muscle during recovery from nonweight bearing

1989 ◽  
Vol 66 (6) ◽  
pp. 2782-2787 ◽  
Author(s):  
E. J. Henriksen ◽  
C. R. Kirby ◽  
M. E. Tischler
Keyword(s):  
Soleus Muscle ◽  
Enzyme Activities ◽  
Glycogen Phosphorylase ◽  
Time Course ◽  
Glycogen Synthase ◽  
Hindlimb Suspension ◽  
Phosphorylase Activity ◽  
Rat Soleus Muscle ◽  
Made In

The time course of glycogen changes in soleus muscle recovering from 3 days of nonweight bearing by hindlimb suspension was investigated. Within 15 min and up to 2 h, muscle glycogen decreased. Coincidentally, muscle glucose 6-phosphate and the fractional activity of glycogen phosphorylase, measured at the fresh muscle concentrations of AMP, increased. Increased fractional activity of glycogen synthase during this time was likely the result of greater glucose 6-phosphate and decreased glycogen. From 2 to 4 h, when the synthase activity remained elevated and the phosphorylase activity declined, glycogen levels increased (glycogen supercompensation). A further increase of glycogen up to 24 h did not correlate with the enzyme activities. Between 24 and 72 h, glycogen decreased to control values, possibly initiated by high phosphorylase activity at 24 h. At 12 and 24 h, the inverse relationship between glycogen concentration and the synthase activity ratio was lost, indicating that reloading transiently uncoupled glycogen control of this enzyme. These data suggest that the activities of glycogen synthase and phosphorylase, when measured at physiological effector levels, likely provide the closest approximation to the actual enzyme activities in vivo. Measurements made in this way effectively explained the majority of the changes in the soleus glycogen content during recovery from nonweight bearing.

Contractile properties of the isolated rat soleus muscle and its single skinned soleus fibers at the early stage of gravitational unloading: Facts and hypotheses

BIOPHYSICS ◽  
2008 ◽  
Vol 53 (6) ◽  
pp. 615-620 ◽  
Author(s):  
E. V. Ponomareva ◽  
V. V. Kravtsova ◽  
E. V. Kachaeva ◽  
E. G. Altaeva ◽  
I. M. Vikhlyantsev ◽  
...  
Keyword(s):  
Soleus Muscle ◽  
Early Stage ◽  
Contractile Properties ◽  
Gravitational Unloading ◽  
Rat Soleus Muscle ◽  
Isolated Rat
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