The study of RNA markers in blood of patients with malignant tumors of gastrointestinal tract

2010 ◽  
Vol 4 (2) ◽  
pp. 209-212
Author(s):  
A. G. Globa ◽  
V. S. Demidova ◽  
O. N. Dikova ◽  
V. A. Vishnevskii ◽  
A. I. Schegolev
Keyword(s):  
Gastrointestinal Tract ◽  
Malignant Tumors

Application of Immunohistochemistry to Liver and Gastrointestinal Neoplasms: Liver, Stomach, Colon, and Pancreas

2008 ◽  
Vol 132 (3) ◽  
pp. 490-499 ◽  
Author(s):  
Stephen A. Geller ◽  
Deepti Dhall ◽  
Randa Alsabeh
Keyword(s):  
Gastrointestinal Tract ◽  
Personal Experience ◽  
Recent Literature ◽  
Malignant Tumors ◽  
Chemotherapeutic Agents ◽  
Gastrointestinal System ◽  
Data Sources ◽  
Gastrointestinal Neoplasms ◽  
Metastatic Neoplasm ◽  
Selection Of

Abstract Context.—Immunohistochemistry has become an integral component of the practice of pathology. Newer antibodies allow for increasingly precise diagnoses for tumors that previously could not be easily identified. Recently, immunohistochemical evaluations have begun to allow pathologists to actively assist in determining prognosis and even in selecting therapies. Objective.—To summarize the usefulness of currently available immunostains for the study of liver and gastrointestinal system neoplasms and to make recommendations for panels of immunostains that can be particularly helpful. Data Sources.—Information has been collected from recent literature as well as from personal experience and practice. Conclusions.—Many immunostains are now available for the practicing pathologist that allow for increasing accuracy in diagnosis of liver and gastrointestinal tract neoplasms. Panels of immunostains can be used to differentiate between various tumors and also to identify site of origin in the case of a metastatic neoplasm. Immunostains that allow for prognostic determinations and for guidance in the selection of chemotherapeutic agents can also be used by pathologists to assist in the management of patients with malignant tumors affecting the liver and gastrointestinal tract.

scholarly journals Value of artificial ascites to assist thermal ablation of liver cancer adjacent to the gastrointestinal tract in patients with previous abdominal surgery

2020 ◽  
Author(s):  
Qiannan Huang ◽  
Jianguo Li ◽  
Qingjing Zeng ◽  
Lei Tan ◽  
Rongqin Zheng ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Liver Cancer ◽  
Abdominal Surgery ◽  
Thermal Ablation ◽  
Malignant Tumors ◽  
Intestinal Fistula ◽  
Previous Abdominal Surgery ◽  
Artificial Ascites ◽  
Major Complications ◽  
Technical Efficacy

Abstract Background To evaluate the feasibility and effectiveness of artificial ascites to assist thermal ablation of liver cancer adjacent to the gastrointestinal tract in patients with previous abdominal surgery. Methods Thirty-nine patients with a total of 40 liver malignant tumors were enrolled between January 2016 and June 2019. All had histories of hepatectomy, splenectomy, cholecystectomy, and intestinal surgery. The distance between the tumor and the gastrointestinal tract was < 5 mm. Normal saline was used as artificial ascites to protect the gastrointestinal tract during thermal ablation. The success rate of the procedure, incidence of major complications, and the technical efficacy of ablation were recorded. Patients were followed for local tumor progression (LTP), and overall survival (OS). Results The use of artificial ascites was successful in 38 of the 40 procedures (95%). Major complications occurred in two of the 39 patients (5.1%) following the procedure. One was an intestinal fistula that occurred in a failed case and was associated with an infection. The other was a liver abscess that occurred in a successful case. The technical efficacy of ablation was 100% (40/40 procedures). The median follow-up was 16 months. The 1-, 2-, and 3-year LTP rates were 2.9%, 5.7% and 5.7%. The 1-, 2-, and 3-year OS rates were 97.1%, 86.8% and 69.5%. Conclusion In patients with previous abdominal surgery, artificial ascites is feasible and effective for assisting thermal ablation of liver cancer adjacent to the gastrointestinal tract.

scholarly journals The study of RNA markers in blood of patients with malignant tumors of gastrointestinal tract

2010 ◽  
Vol 56 (4) ◽  
pp. 510-516 ◽  
Author(s):  
A.G. Globa ◽  
V.S. Demidova ◽  
O.N. Dikova ◽  
V.A. Vishnevskiy ◽  
A.I. Shchegolyev
Keyword(s):  
Gastrointestinal Tract ◽  
Malignant Tumors ◽  
Capillary Blood ◽  
Marker Genes ◽  
Mrna Levels ◽  
Cytokeratin 20 ◽  
Reaction Products ◽  
Specificity And Sensitivity ◽  
Primary Diagnostics

In order to develop a diagnostic panel, mRNA levels of tumor marker genes have been evaluated in capillary blood of patients with various malignant tumors of the gastrointestinal tract (GIT) by means of the method of reverse transcription combined with real-time PCR with detection of reaction products using TaqMan probes. Use of small volumes of capillary blood did not decrease sensitivity of this method. RNA expression of telomerase (mhTERT), alpha-fetoprotein (mAFP), carcinoembryonic antigen (mCEA) and cytokeratin-20 (mCK-20) was higher in most patients with tumors. Blood of donors or non-oncological patients contained much lower (trace) amounts of the RNA markers. The RNA markers are characterized by reasonably high specificity and sensitivity acceptable for diagnostic application. The mhTERT marker was the most universal one and exhibited the highest specificity and sensitivity. Combined determination of several RNA markers increased sensitivity of this method. It is concluded that determination of RNA markers in small volumes of capillary blood may be used for screening, primary diagnostics, and postoperative monitoring.

THE INFORMATION CONTENT AND CORRELATION MODEL OF INTERLEUKINS, INDICATORS OF OXIDATIVE AND ANTIOXIDATIVE SYSTEMS IN MALIGNANT TUMORS OF THE GASTROINTESTINAL TRACT

2019 ◽  
Vol 65 (1) ◽  
pp. 126-130
Author(s):  
Nikolay Agarkov ◽  
Kristina Makkonen ◽  
Pavel Tkachenko ◽  
Vitaliy Aksenov ◽  
Aleksandr Ivanov ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Glutathione Peroxidase ◽  
Information Content ◽  
Malignant Tumors ◽  
Cytokine Profile ◽  
Antioxidant Systems ◽  
Correlation Model ◽  
Healthy Individuals ◽  
Glutathione S Transferase ◽  
Antioxidative Systems

According to the results of a survey of 76 patients in malignant tumors of the gastrointestinal tract and 30 nearly healthy individuals found that the most informative in the diagnostic of this pathology have IL-2, IL-4, IL-12, glutathione-S-transferase and glutathione peroxidase. These indicators of cytokine profile and antioxidant systems in malignant tumors of the gastrointestinal tract characterized by the presence of a significant number of reliable intersystem relations. Development in malignant tumors of the gastrointestinal tract is accompanied by increased intra-conjugacy parameters of various interleukins and antioxidant systems.

scholarly journals Usage characteristics and adverse event rates ​of the direct puncture and pull techniques for percutaneous endoscopic gastrostomy in patients with malignant tumors of the upper aerodigestive tract

2018 ◽  
Vol 06 (01) ◽  
pp. E29-E35 ◽  
Author(s):  
Niels Teich ◽  
Lars Selig ◽  
Susanne Liese ◽  
Franziska Schiefke ◽  
Alexander Hemprich ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Adverse Events ◽  
Percutaneous Endoscopic Gastrostomy ◽  
Malignant Tumors ◽  
Short Term ◽  
Direct Puncture ◽  
Endoscopic Gastrostomy ◽  
Upper Gastrointestinal

Abstract Background and study aims Patients with malignant tumors of the upper gastrointestinal tract are at risk of weight loss. Early supportive nutrition therapy is therefore recommended and usually requires placement of a percutaneous endoscopic gastrostomy (PEG). The aim of this study was to compare adverse events and usage characteristics of the direct puncture technique with those of the traditional pull technique when used in patients with endoscopically passable tumors. The primary endpoint was the rate of inflammatory adverse events (AEs) at the gastrostomy fistula. The secondary endpoint was the long-term rate of puncture-site metastases. Patients and methods One hundred twenty patients (median age 56; IQR 36, 86 years) were randomized and treated per protocol in this prospective open randomized single-center study. Follow-ups were conducted on the third and seventh post-interventional days, after 1, 3 and 6 months and the last follow-up 5 years after intervention. Results Within the short-term follow-up period of 6 months after PEG placement, AEs were noted in 47 patients (39.2 %). These included 22 inflammations and 16 device dislocations and were mainly found in the puncture group (33 vs. 14 in the pull group) with a significantly increased incidence in the first month after PEG insertion (P = 0.001). Evaluation of the 5-year data did not reveal any significant differences. The gastrostomy tube was used in 101 patients (84.2 %) (range 18 days to 5 years). Conclusions Our results favor the pull technique for patients with endoscopically passable tumors of the upper gastrointestinal tract due to less short-term adverse events. Both systems contributed equally to secure long-term use.

scholarly journals A Survival Analysis of Chinese Patients with Gastrointestinal Mucosal Melanomas: A Single-Centre Retrospective Study

2021 ◽  
pp. 1-7
Author(s):  
Manxia Lin ◽  
Jiayao Huang ◽  
Junrong Chen ◽  
Xiaoying Lou ◽  
Yi Lu ◽  
...  
Keyword(s):  
Multivariate Analysis ◽  
Retrospective Study ◽  
Gastrointestinal Tract ◽  
Proportional Hazards Model ◽  
Malignant Tumors ◽  
Metastatic Tumor ◽  
Tumor Location ◽  
Cox Proportional Hazards Model ◽  
Chinese Patients ◽  
Primary Tumors

Aim: Mucosal melanomas are highly malignant tumors that are of great interest for their aggressive behaviour and unfavourable prognosis, which could be the result of many reasons. In this paper, we describe a retrospective study performed to investigate the characteristics and prognoses of gastrointestinal mucosal melanomas in a Chinese population to help future clinicians recognize the prognosis of this disease. Methods: We retrospectively studied 49 patients diagnosed with gastrointestinal mucosal melanomas. Multivariate analysis of prognosis and overall survival (OS) was performed using the Cox proportional hazards model. Results: The multivariate analysis showed that the condition of the primary or metastatic tumor and tumor location were independent factors that affected the prognosis of patients with gastrointestinal mucosal melanomas. Patients with metastatic tumors had a better prognosis than patients with primary tumors, and tumors that occurred in the lower gastrointestinal tract had a better prognosis than those that occurred in the upper gastrointestinal tract. Conclusion: Tumor location and the condition of the primary or metastatic tumor may be independent factors that affect the prognosis of patients with gastrointestinal mucosal melanomas.

scholarly journals Value of artificial ascites to assist thermal ablation of liver cancer adjacent to the gastrointestinal tract in patients with previous abdominal surgery

2020 ◽  
Author(s):  
Qiannan Huang ◽  
Jianguo Li ◽  
Qingjing Zeng ◽  
Lei Tan ◽  
Rongqin Zheng ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Liver Cancer ◽  
Abdominal Surgery ◽  
Thermal Ablation ◽  
Malignant Tumors ◽  
Intestinal Fistula ◽  
Previous Abdominal Surgery ◽  
Artificial Ascites ◽  
Major Complications ◽  
Technical Efficacy

Abstract BackgroundTo evaluate the feasibility and effectiveness of artificial ascites to assist thermal ablation of liver cancer adjacent to the gastrointestinal tract in patients with previous abdominal surgery.MethodsThirty-nine patients with a total of 40 liver malignant tumors were enrolled between January 2016 and June 2019. All had histories of hepatectomy, splenectomy, cholecystectomy, and intestinal surgery. The distance between the tumor and the gastrointestinal tract was < 5 mm. Normal saline was used as artificial ascites to protect the gastrointestinal tract during thermal ablation. The success rate of the procedure, incidence of major complications, and the technical efficacy of ablation were recorded. Patients were followed for local tumor progression (LTP), and overall survival (OS).ResultsThe use of artificial ascites was successful in 38 of the 40 procedures (95%). Major complications occurred in two of the 39 patients (5.1%) following the procedure. One was an intestinal fistula that occurred in a failed case and was associated with an infection. The other was a liver abscess that occurred in a successful case. The technical efficacy of ablation was 100% (40/40 procedures). The median follow-up was 20 months. The 1-, 2-, and 3-year LTP rates were 2.9%, 5.7% and 5.7%. The 1-, 2-, and 3-year OS rates were 97.1%, 86.8% and 69.5%.ConclusionIn patients with previous abdominal surgery, artificial ascites is feasible and effective for assisting thermal ablation of liver cancer adjacent to the gastrointestinal tract.Co-first authors: Qiannan Huang #, Jianguo Li # contributed equally to this article and should be considered co-first authors.Co-corresponding authors: Xuqi He *, Kai Li * contributed equally to this article and should be considered co-corresponding authors.

scholarly journals Histopathological Distribution of the Gastrointestinal Tract Lesions

2021 ◽  
Vol 6 (2) ◽  
pp. 1529-1534
Author(s):  
Mrinalini Singh ◽  
Santosh Upadhyaya Kafle ◽  
Neeta Kafle ◽  
Amrita Sinha ◽  
Prasun Rajbhandari
Keyword(s):  
Gastrointestinal Tract ◽  
Malignant Tumors ◽  
Premalignant Lesions ◽  
Medical College ◽  
Gastrointestinal Lesions ◽  
Stomach Pain ◽  
Life Threatening ◽  
Hematoxylin And Eosin Stain ◽  
A Prospective Study ◽  
Clinical Conditions

Introduction: Gastrointestinal (GI) diseases are common and can affect any portion of the gastrointestinal tract from the mouth to the anus.  Diseases can just shows clinical conditions like stomach pain, constipation, diarrhea which can be self- limiting. But sometimes disease may be life-threatening like malignancy. Biopsy is necessary for confirmatory diagnosis and further treatment of the patient. So histopathologic examination is  a must for all surgical procedures for confirmation and categorization of GI disorders. Objectives: This study was done to find out the various patterns of lesions of the gastrointestinal tract with its commonest age group and sex involvement Methodology: This was a prospective study of all the surgically resected GI tissue received in the Department of Pathology Histopathology unit in Birat Medical College and Teaching Hospital (BMCTH) from 1st February 2021 to 30 thApril 2021.The hematoxylin and eosin stain slides of the GI tissue received were studied and the lesions were diagnosed on their histomorphology.  According to organ, age and sex, the lesions were categorized. The data were entered in Microsoft excel and the percentage value was calculated. Results: Out of the total 344 cases 146(42.44%) were male and 198(57.56%) were female patients. The most common age range for GI lesions was 41 to 60 years comprising of 126(36.62%) of total cases. Maximum numbers of cases 160 (46.50%) were of cholecystectomies followed by appendectomies 95(27.60%). Inflammatory and benign lesions comprised 332(96.51%), 9(2.61%) were malignant tumor and 3(0.88%) were premalignant lesions. The most common inflammatory lesions and malignant tumors were chronic cholecystitis and gastric adenocarcinoma respectively Conclusions: The study identifies that gastrointestinal lesions comprise of the most common biopsies received in the histopathology department. Early diagnosis of premalignant and malignant lesions can improve the overall survival rate of patients.

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