Effect of Mongolian warm acupuncture on the gene expression profile of rats with insomnia

2019 ◽  
Vol 37 (5) ◽  
pp. 301-311
Author(s):  
Gula A ◽  
Xian Li ◽  
Budao Su ◽  
Hua Lian ◽  
Manjie Bao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Rat Model ◽  
Quantitative Polymerase Chain Reaction ◽  
Control Group ◽  
Expression Of Genes ◽  
Pathways Analysis ◽  
Micro Array ◽  
Analysis System

Background: The mechanism of Mongolian warm acupuncture (MWA) for the treatment of insomnia has not been previously reported. Objective: To investigate the effect of MWA on gene expression profile in the p-chlorophenylalanine (PCPA)-induced rat model of insomnia. Methods A rat model of insomnia was established and the animals were divided into five groups: control, PCPA (untreated), PCPA+estazolam, PCPA+MA (manual acupuncture), and PCPA+MWA. The rats were euthanased at 7 days after treatment, and hypothalamic tissue was harvested to extract total RNA for the analysis of gene expression profile. Micro-array and Partek Genomics Suite analysis system were used to analyse differential expression of genes between groups. Furthermore, ingenuity pathways analysis was used to analyse the main regulators. Results: After treatment, in rats with improved sleep, micro-array data from the follow-up phase compared with baseline showed that MWA down-regulated 11 genes compared with the control group and 16 genes compared with the PCPA group. Six genes were selected following the micro-array detection to perform quantitative polymerase chain reaction (qPCR) verification, and the results showed that the coincidence rate was up to 90%, which verified the reliability of the microarray results. Compared with the PCPA group, transcription levels of Egr 1, Btg2 and BDNF in the PCPA+MWA group were up-regulated (P<0.05). Conclusion: In combination, the findings of this study suggests that MWA is efficacious at improving sleep in an experimental rat model of insomnia.

scholarly journals Estudio post-mortem de las alteraciones en la expresión de RNA en el cerebro de pacientes suicidas

2020 ◽  
Author(s):  
Brenda Cabrera-Mendoza
Keyword(s):  
Gene Expression ◽  
Prefrontal Cortex ◽  
Gene Expression Profile ◽  
Dual Diagnosis ◽  
Expression Profile ◽  
Suicidal Behavior ◽  
Suicide Risk ◽  
Dual Pathology ◽  
Expression Of Genes ◽  
The Brain

Despite individuals with substance use disorder (SUD) have a high suicide risk, most of gene expression studies in suicide have excluded individuals with this disorder. Thus, little is known about the gene expression profile in suicides with SUD. The identification of altered biological processes in the brain of suicides with SUD is crucial in the comprehension of the SUD and suicidal behavior comorbidity. This dissertation describes the evaluation of gene expression differences in the dorsolateral prefrontal cortex of suicides and non-suicides with and without SUD.Sixty-six brain tissue samples were collected and classified in the following groups: i) 23 suicides with SUD, ii) 20 suicides without SUD, iii) 9 non-suicides with SUD and iv) 14 non-suicides without SUD. The results of this study suggest that suicides with SUD have a gene expression profile in the prefrontal cortex different from that of individuals with only one of these conditions, presenting differences in the expression of genes involved in cell proliferation and glutamatergic neurotransmission.We performed a re-analysis of the gene expression data of 38 suicides focused on dual diagnosis and suicide. Dual diagnosis is the concurrence of at least one SUD and one or more mental disorders in a given individual. Although this comorbidity is highly prevalent and is associated with adverse clinical outcomes, its neurobiology has not been elucidated. In addition, patients with dual pathology have a higher suicide risk compared to patients with only one disorder.The objective of this re-analysis was to evaluate the differences in the gene expression profile in the prefrontal cortex of suicides with dual pathology compared to suicides with a single disorder. Our results suggest an alteration in the expression of genes involved in glutamatergic neurotransmission, GABAergic neurotransmission and neurogenesis in suicides with dual diagnosis compared to suicides with a single disorder and suicides without mental comorbidities.The observed differences in gene expression in the prefrontal cortex between suicides with and without SUD, as well as suicides with dual diagnosis and a single disorder may contribute to the phenotypic and clinical discrepancies observed among these patients. The identification of molecular characteristics in the brain of individuals with suicidal behavior and psychiatric comorbidities will allow the design of preventive and therapeutic measures aimed at the adequate treatment of each comorbidity.

Expression of angiogenesis-related genes in a group of Iranian cases of breast cancer

2020 ◽  
Vol 17 ◽  
Author(s):  
Seyyed Amir Yasin Ahmadi ◽  
Soheila Sayad ◽  
Farhad Shahsavar ◽  
Reza Nekouian ◽  
Mahshid Panahi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Targeted Therapy ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Quantitative Polymerase Chain Reaction ◽  
Molecular Profile ◽  
Benign Tumors ◽  
Study Gene Expression ◽  
Molecular Etiology

Aims: To design an angiogenesis gene expression profile; to study angiogenesis gene expression profile in breast cancer; to map angiogenesis gene expression profile in individual participants. Background: In molecular etiology of each disease, there are some important molecules involved in the related pathways. From the viewpoint of precision medicine, molecular etiology of a disease is different person by person because genetic variations of the genes involved in these pathways. This point of view intend researchers of drug development to design novel drugs for targeted therapy based on the exact etiology. In the case of angiogenesis, there is a drug profile parallel to the molecular profile. Bevacizumab, sunitinib and aflibercept are the examples of anti-angiogenic drugs. Objectives: A hallmark of solid tumors is sustained angiogenesis. Vascular endothelial growth factors (VEGF), VEGF receptors (VEGFR) and placental growth factor (PlGF) are involved in angiogenesis. We aimed to study gene expression profile of angiogenesis including VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGFR-1, VEGFR-2, VEGFR-3 and PlGF in an Iranian group of patients undergoing breast surgery due to breast cancer and breast fibroadenoma. Methods: Tumor tissue samples of a group of patients with invasive ductal carcinoma (IDC) and a group of patients with fibroadenoma (Fib) were used. Gene expression was studied by real-time quantitative polymerase chain reaction (q-PCR) and fold changes (FC) with their 95% confidence intervals (CI) were reported based on calibration with normal breast tissue. Results: All the genes showed significant up regulation in IDC group. The most up regulation was for VEGFR-2 (FC=52.68; 95% CI=17.96-154.47; P<0.001). In Fib group, PlGF showed a significant up regulation (FC=10.41; 95% CI=5.35-20.26; P=0.002). Comparison of IDC group with Fib group showed significant up regulation of VEGFR-1, VEGFR-2 and VEGFR-3 in IDC group (P<0.05). Conclusion: Malignancy of breast tumors is associated with over expression of all the genes of this profile. However, only VEGFRs showed up regulation in comparison to benign tumors. Individualized targeted therapy according to this profile should be studied in future.

scholarly journals Global Gene Expression Profile of the Hippocampus in a Rat Model of Vascular Dementia

2015 ◽  
Vol 237 (1) ◽  
pp. 57-67 ◽  
Author(s):  
Lin Wu ◽  
Xiao-Tao Feng ◽  
Yue-Qiang Hu ◽  
Nong Tang ◽  
Qing-Shan Zhao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Vascular Dementia ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Rat Model ◽  
Global Gene Expression ◽  
Global Gene Expression Profile

Age-dependent gene expression profile and protein expression in a transgenic rat model of Huntington's disease

2008 ◽  
Vol 2 (12) ◽  
pp. 1638-1650 ◽  
Author(s):  
Huu Phuc Nguyen ◽  
Silke Metzger ◽  
Carsten Holzmann ◽  
Dirk Koczan ◽  
Hans-Jürgen Thiesen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Protein Expression ◽  
Huntington's Disease ◽  
Huntington’S Disease ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Rat Model ◽  
Transgenic Rat ◽  
Age Dependent ◽  
Transgenic Rat Model

scholarly journals Effects of histamine and various histamine receptor antagonists on gene expression profiles of macrophages during compressive strain

2021 ◽  
Author(s):  
Agnes Schröder ◽  
Catharina Petring ◽  
Anna Damanaki ◽  
Jonathan Jantsch ◽  
Peter Proff ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Periodontal Ligament ◽  
Tooth Movement ◽  
Expression Profiles ◽  
Quantitative Polymerase Chain Reaction ◽  
Compressive Strain ◽  
Orthodontic Tooth Movement ◽  
The Impact

Abstract Purpose Tissue hormone histamine can accumulate locally within the periodontal ligament via nutrition or may be released during allergic reactions by mast cells, which may have an impact on orthodontic tooth movement. In addition to periodontal ligament fibroblasts, cells of the immune system such as macrophages are exposed to compressive strain. The aim of this study was thus to investigate the impact of histamine on the gene expression profile of macrophages in the context of simulated orthodontic compressive strain. Methods Macrophages were incubated with different histamine concentrations (50, 100, 200 µM) for 24 h and then either left untreated or compressed for another 4 h. To assess the role of different histamine receptors, we performed experiments with antagonists for histamine 1 receptor (cetirizine), histamine 2 receptor (ranitidine) and histamine 4 receptor (JNJ7777120) under control and pressure conditions. We tested for lactate dehydrogenase release and analyzed the expression of genes involved in inflammation and bone remodeling by reverse transcription quantitative polymerase chain reaction (RT-qPCR). Results Histamine elevated gene expression of tumor necrosis factor under control conditions and in combination with pressure application. Increased prostaglandin-endoperoxide synthase‑2 mRNA was observed when histamine was combined with compressive force. Interleukin‑6 gene expression was not affected by histamine treatment. In macrophages, compressive strain increased osteoprotegerin gene expression. Histamine further elevated this effect. Most of the observed histamine effects were blocked by the histamine 1 receptor antagonist cetirizine. Conclusions Histamine has an impact on the gene expression profile of macrophages during compressive strain in vitro, most likely having an impairing effect on orthodontic tooth movement by upregulation of osteoprotegerin expression.

The Oncocytic Variant of Poorly Differentiated Thyroid Carcinoma Shows a Specific Immune-Related Gene Expression Profile

2020 ◽  
Vol 105 (12) ◽  
pp. e4577-e4592
Author(s):  
Jasna Metovic ◽  
Chiara Vignale ◽  
Laura Annaratone ◽  
Simona Osella-Abate ◽  
Francesca Maletta ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Adverse Outcome ◽  
Control Group ◽  
Related Gene ◽  
Molecular Features ◽  
Immune Related Gene ◽  
Oncocytic Variant ◽  
Poorly Differentiated

Abstract Background Poorly differentiated thyroid cancer (PDTC) is a rare, follicular cell-derived neoplasm with an unfavorable prognosis. The oncocytic variant of PDTC may be associated with even more adverse outcome than classical PDTC cases, but its specific molecular features are largely unknown. Our aim was to explore the immune-related gene expression profile of oncocytic and classical PDTC, in correlation with clinical and pathological characteristics (including programmed death ligand 1 [PD-L1] expression) and outcome, and in comparison with a control group of well-differentiated follicular carcinomas (WDFCs), including conventional follicular carcinomas (FTCs) and Hürthle cell carcinomas (HCCs). Methods A retrospective series of 48 PDTCs and 24 WDFCs was analyzed by means of NanoString technology employing the nCounter PanCancer Immune Profiling panel. Gene expression data were validated using quantitative real-time polymerase chain reaction. Results Oncocytic PDTCs showed a specific immune-related gene expression profile, with higher expression of LAIR2, CD274, DEFB1, IRAK1, CAMP, LCN2, LY96, and APOE, and lower expression of NOD1, as compared to conventional PDTCs. This molecular signature was associated with increased intratumoral lymphocytic infiltration, PD-L1 expression, and adverse outcome. Three of these genes, CD274, DEFB1, and IRAK1, as well as PD-L1 expression, were also the hallmarks of HCCs as compared to FTCs. By contrast, the panel of genes differentially regulated in PDTCs as compared to WDFCs was unrelated to the oncocytic phenotype. Conclusions Our results revealed a distinctive immune-related gene expression profile of oncocytic PDTC and confirmed a more aggressive outcome in this cancer subtype. These findings may provide guidance when exploring novel immunotherapeutic options for oncocytic PDTC patients.

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