scholarly journals 937 Advanced understanding of the tumor microenvironment with multiplex analysis: an automated 7-color multiplex assay using Akoya’s opal technology

2021 ◽  
Vol 9 (Suppl 3) ◽  
pp. A983-A983
Author(s):  
Bhavika Patel ◽  
Stephanie Allen ◽  
Tania Eliseeva ◽  
Najiba Mammadova ◽  
Agnes Haggerty ◽  
...  
Keyword(s):  
Image Analysis ◽  
Immune System ◽  
Tumor Microenvironment ◽  
Cancer Therapy ◽  
Precision Medicine ◽  
Quantitative Data ◽  
Multiplex Assay ◽  
Multiplex Immunoassay ◽  
Adaptive Immune ◽  
Insight Into

BackgroundImmunotherapy and precision medicine are rapidly developing approaches to cancer therapy. Biomarkers that detect the tumor and tumor microenvironment allow for the development of strategies that accelerate the development of treatments that enhance a patient‘s immune system. Akoya’s MOTiF™ PD-1/PD-L1 Panel is a validated, multiplex immunoassay enabling detection of the 6 most clinically relevant immuno-oncology and spatial markers: PD-1, PD-L1, FoxP3, CD8, CD68, and PanCK. This panel provides unparalleled quantitative data for pre-clinical and translational IO research.MethodsThe MOTiF™ PD-1/PD-L1 Panel was used to stain normal and tumor lung tissues. Stained slides were analyzed using the InForm® and Visiopharm® image analysis platforms.ResultsWe introduce the workflow and image analysis solutions using InForm® and Visiopharm® software to provide robust, quantifiable data.ConclusionsThis data provides insight into the innate and adaptive immune environment for targeted design of new immunotherapies. These new targeted immunotherapies could potentially improve efficacy and reduce toxicity.

scholarly journals SARS-COV-2 acts as a test of Air Pollution, Lifestyle Disorders and Immune Tolerance dysregulation in human body

2021 ◽  
Author(s):  
bose Karthik
Keyword(s):  
Immune System ◽  
Immune Tolerance ◽  
Adaptive Immune System ◽  
Innate Immune ◽  
Human Immune System ◽  
Adaptive Immune ◽  
Human Immune ◽  
Humoral Responses ◽  
Skeletal Mechanism ◽  
Insight Into

SARS-COV-2 is reported to be associated with severe immune dysregulation, delayed humoral responses and accelerated innate immune response mediated damages. As the pandemic is turning the world upside down, In order to address this disease we should first get an insight into the mechanism of action through which SARS-COV-2 is achieving the above said dysregulating or modulating effects on human immune system. T his article presents the basic or skeletal mechanism through which SARS-COV-2 dysregulates immune system by targeting innate immune system, adaptive immune system and different immune tolerance check points by dysregulating different miRNA’s and the preexisting conditions or comorbidities of the patients. This article comprises of the comparative and comprehensive literature review targeting all topics with the data available/reported till date in the scientific community.

scholarly journals The innate and adaptive infiltrating immune systems as targets for breast cancer immunotherapy

2017 ◽  
Vol 24 (4) ◽  
pp. R123-R144 ◽  
Author(s):  
Andrew M K Law ◽  
Elgene Lim ◽  
Christopher J Ormandy ◽  
David Gallego-Ortega
Keyword(s):  
Breast Cancer ◽  
Immune System ◽  
Cancer Therapy ◽  
Cancer Cell ◽  
Innate Immune System ◽  
Cancer Biology ◽  
Adaptive Immune System ◽  
Cell Types ◽  
Innate Immune ◽  
Adaptive Immune

A cancer cell-centric view has long dominated the field of cancer biology. Research efforts have focussed on aberrant cancer cell signalling pathways and on changes to cancer cell DNA. Mounting evidence demonstrates that many cancer-associated cell types within the tumour stroma co-evolve and support tumour growth and development, greatly modifying cancer cell behaviour, facilitating invasion and metastasis and controlling dormancy and sensitivity to drug therapy. Thus, these stromal cells represent potential targets for cancer therapy. Among these cell types, immune cells have emerged as a promising target for therapy. The adaptive and the innate immune system play an important role in normal mammary development and breast cancer. The number of infiltrating adaptive immune system cells with tumour-rejecting capacity, primarily, T lymphocytes, is lower in breast cancer compared with other cancer types, but infiltration occurs in a large proportion of cases. There is strong evidence demonstrating the importance of the immunosuppressive role of the innate immune system during breast cancer progression. A consideration of components of both the innate and the adaptive immune system is essential for the design and development of immunotherapies in breast cancer. In this review, we focus on the importance of immunosuppressive myeloid-derived suppressor cells (MDSCs) as potential targets for breast cancer therapy.

scholarly journals INSIGHT INTO COMPLETE BLOOD COUNT IN MALIGNANCIES

2019 ◽  
pp. 1-2
Author(s):  
Gayathri M. N
Keyword(s):  
Immune System ◽  
Tumor Microenvironment ◽  
Blood Count ◽  
Complete Blood Count ◽  
Metastatic Cancer ◽  
Hematological Parameters ◽  
Statistical Correlation ◽  
Physiological Process ◽  
Host Interaction ◽  
Insight Into

It is very well known that the tumor-host interaction is not limited to the tumor microenvironment but to also alter the entire physiological process to help the development and progression of cancer. Our immune system does not escape from the clutches of the cancer, i.e altered hematological parameters can influence the progression of cancer and vice versa. With this in mind we conducted this study in the Department of Pathology MMC&RI with an attempt to reveal the pattern of complete blood count in malignancies and their difference in localized and metastatic cancer. All the cases included in this study were the ones referred to Cytopatholgy, and diagnosed with cancer. There was significant statistical correlation between the various hematological parameters and the metastatic cancers implying their use to predict the tumor behavior

Morphometric analysis of cellular interactions with the endothelium during the development of arterial lesions using Scanning Electron Microscopy and digital image analysis

1987 ◽  
Vol 45 ◽  
pp. 918-919
Author(s):  
M.E. Rosenfeld ◽  
C. Karboski ◽  
M.F. Prescott ◽  
P. Goodwin ◽  
R. Ross
Keyword(s):  
Electron Microscopy ◽  
Image Analysis ◽  
Quantitative Data ◽  
Digital Image Analysis ◽  
Lower Cost ◽  
Matched Control ◽  
Digital Analysis ◽  
Cellular Interactions ◽  
Arterial Lesions ◽  
Scanning Electron

Previous research documenting the chronology of the cellular interactions that occur on or below the surface of the endothelium during the initiation and progression of arterial lesions, primarily consisted of descriptive studies. The recent development of lower cost image analysis hardware and software has facilitated the collection of high resolution quantitative data from microscopic images. In this report we present preliminary quantitative data on the sequence of cellular interactions that occur on the endothelium during the initiation of atherosclerosis or vasculitis utilizing digital analysis of images obtained directly from the scanning electron microscope. Segments of both atherosclerotic and normal arteries were obtained from either diet-induced or endogenously (WHHL) hypercholesterolemic rabbits following 1-4 months duration of hypercholesterolemia and age matched control rabbits. Vasculitis was induced in rats following placement of an endotoxin soaked thread adjacent to the adventitial surface of arteries.

Evolution and age characteristics of the innate and adaptive immune system

2016 ◽  
Vol 75 (3) ◽  
pp. 74-84 ◽  
Author(s):  
A.E. Abaturov ◽  
◽  
E.A. Agafonova ◽  
N.I. Abaturova ◽  
V.L. Babich ◽  
...  
Keyword(s):  
Immune System ◽  
Adaptive Immune System ◽  
Adaptive Immune
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