The ectomycorrhizal status of Calostoma cinnabarinum determined using isotopic, molecular, and morphological methods

2007 ◽  
Vol 85 (4) ◽  
pp. 385-393 ◽  
Author(s):  
Andrew W. Wilson ◽  
Erik A. Hobbie ◽  
David S. Hibbett
Keyword(s):  
Ectomycorrhizal Fungi ◽  
Internal Transcribed Spacer ◽  
Morphological Characters ◽  
Soil Cores ◽  
Fruiting Bodies ◽  
Root Tips ◽  
Specific Primers ◽  
Saprotrophic Fungi ◽  
Plant Associations ◽  
Ectomycorrhizal Basidiomycetes

Calostoma cinnabarinum Corda belongs to the suborder Sclerodermatineae (Boletales), which includes many well-known ectomycorrhizal basidiomycetes, but the genus Calostoma has been described as saprotrophic. This study combines isotopic, molecular, and morphological techniques to determine the mode of nutrition of C. cinnabarinum. δ13C and δ15N measurements were compared among co-occurring C. cinnabarinum, ectomycorrhizal fungi, saprotrophic fungi, and ectomycorrhizal plants. Isotopic profiles of C. cinnabarinum resembled those of ectomycorrhizal fungi but not those of saprotrophic fungi. For molecular analyses, ectomycorrhizal root tips were extracted from soil cores collected beneath C. cinnabarinum fruit bodies. Nuclear ribosomal internal transcribed spacer (nrITS) sequences were obtained from ectomycorrhizal root tips using fungal-specific primers and screened against C. cinnabarinum nrITS sequences. Ectomycorrhizal root tips had nrITS sequences that matched C. cinnabarinum fruiting bodies. Root tips colonized by C. cinnabarinum were also described morphologically. Several morphological characters were shared between fruiting bodies and ectomycorrhizal root tips of C. cinnabarinum. Results of isotopic, molecular, and morphological analyses indicate that C. cinnabarinum is ectomycorrhizal. Molecular analysis and observations of plant associations suggest that C. cinnabarinum forms ectomycorrhizae with Quercus .

scholarly journals Ectomycorrhizal Fungi on South Kalimantan Serpentine Soil

2020 ◽  
Vol 4 (1) ◽  
Author(s):  
Rudy Hermawan ◽  
Witiyasti Imaningsih ◽  
Badruzsaufari Badruzsaufari
Keyword(s):  
Heavy Metals ◽  
Ectomycorrhizal Fungi ◽  
Morphological Characters ◽  
Ectomycorrhizal Fungus ◽  
Serpentine Soil ◽  
Fruiting Bodies ◽  
Genus Identification ◽  
Manganese Chromium ◽  
Cobalt And Nickel

Serpentine soil contains highly heavy metals, such as manganese, chromium, cobalt, and nickel,which could bean inappropriate growthmediaofmostplants. Someplants thatfound able to grow optimally on South Kalimantan serpentine soil have been known to do association with ectomycorrhizal fungi. This research aimed to obtain and characterize mushrooms assumed as ectomycorrhizal fungi indigenous South Kalimantan serpentine soil. This study used field exploration of fungal fruiting bodies and identified the genus based on morphological characters of fruiting bodies such as shape, size, and ornamentation, which are unique for the genus identification, then compared the characteristics on mushroomexpert.com. The mushrooms were also confirmed of genera assumed as ectomycorrhizal fungi based on mycorrhizas.info. Seven fruiting bodies were obtained and classified as Cantharellus (Ct), Chlorophyllum (Ch1 and Ch2), Lycoperdon (Ly), Ramaria (Rm1 and Rm2), and Thelephora (Tp). The results showed that all of those fruiting bodies belong to Basidiomycetes. There were 4 genera of Cantharellus, Lycoperdon, Ramaria, and Thelephora, assumed as ectomycorrhizal fungi. But Chlorophyllum genus was never reported as ectomycorrhizal fungus

Molecular phylogenetic analysis reveals two new species of Discosia from Italy

Phytotaxa ◽  
2015 ◽  
Vol 203 (1) ◽  
pp. 37 ◽  
Author(s):  
Wenjing Li ◽  
Jian-kui Liu ◽  
D. Jayarama Bhat ◽  
Erio Camporesi ◽  
Dong-QING DAI ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Internal Transcribed Spacer ◽  
Large Subunit ◽  
Morphological Characters ◽  
Fruiting Bodies ◽  
Forensic Dna ◽  
Two New Species ◽  
Molecular Phylogenetic ◽  
Large Subunit Rdna

Two fresh collections of Discosia were made from dead leaves of Fagus sylvatica in Italy. As these collections could not be cultured, the fruiting bodies were directly used for sequencing using a Forensic DNA Extraction Kit. Based on analyses of the concatenated internal transcribed spacer regions of the nrDNA operon (ITS) and large subunit rDNA (LSU) gene sequences, as well as morphological characters, the fresh collections are introduced as two new species, namely D. italica and D. fagi. Phylogenetically, these two species are distinct from all other Discosia species. Morphologically, D. italica is somewhat similar with D. fagi, but can be distinguished using dimension of conidiomata and conidiogenous cells. Descriptions and illustrations of the new taxa are provided herein.

scholarly journals Ectomycorrhizal Fungi Associated with Pinus densiflora Seedlings under Flooding Stress

2021 ◽  
Vol 13 (8) ◽  
pp. 4367
Author(s):  
Yoonhee Cho ◽  
Shinnam Yoo ◽  
Myung Soo Park ◽  
Ji Seon Kim ◽  
Chang Sun Kim ◽  
...  
Keyword(s):  
Ectomycorrhizal Fungi ◽  
Internal Transcribed Spacer ◽  
Pinus Densiflora ◽  
Red Pine ◽  
Fungal Communities ◽  
Thelephora Terrestris ◽  
Suillus Luteus ◽  
Flooding Stress ◽  
Pine Seedlings ◽  
Rhizopogon Luteolus

Flooding is an environmental stress for plants that not only limits aeration and nutrient acquisition, but also disturbs underground plant-associated fungal communities. Despite frequent flooding, red pine (Pinus densiflora) seedlings thrive in streamside environments. However, whether the compatible ectomycorrhizal fungi (EMF) of red pine are affected by natural flooding is unclear. As EMF are vital symbionts for the development of many trees and allow them to overcome various environmental stresses, in this study, the EMF species associated with red pine seedlings in a streamside environment in Korea were investigated after flooding. The EMF species in 47 seedlings collected from the streamside site were identified by observing their different morphotypes using internal transcribed spacer sequence analysis, and a total of 10 EMF species were identified. The EMF species diversity was lower than that in samples collected from a nearby forest analyzed as a control. The dominant EMF species of streamside seedlings included Amphinema spp., Rhizopogon luteolus, Suillus luteus, and Thelephora terrestris. This study could serve as a basis for investigating the mechanisms by which advantageous EMF aid plant development under flooding stress.

Ectomycorrhizal fungi with edible fruiting bodies 3.Tuber magnatum, tuberaceae

1998 ◽  
Vol 52 (2) ◽  
pp. 192-200 ◽  
Author(s):  
I. R. Hall ◽  
A. Zambonelli ◽  
F. Primavera
Keyword(s):  
Ectomycorrhizal Fungi ◽  
Fruiting Bodies ◽  
Tuber Magnatum

Fungal composition of lichen thalli assessed by single strand conformation polymorphism

2010 ◽  
Vol 42 (4) ◽  
pp. 461-473 ◽  
Author(s):  
Lucia MUGGIA ◽  
Martin GRUBE
Keyword(s):  
Direct Sequencing ◽  
Its Region ◽  
Single Strand Conformation Polymorphism ◽  
Morphological Characters ◽  
Single Strand ◽  
Specific Primers ◽  
Lichenicolous Fungi ◽  
Large Numbers ◽  
Total Dna ◽  
Conformation Polymorphism

AbstractFungi that are unrelated to the mycobiont species frequently colonize lichens. Some of these fungal colonists are described lichenicolous fungi, lichen parasites and pathogens that produce recognizable morphological characters, while others apparently produce no noticeable structures. Here we apply the single strand conformation polymorphism (SSCP) technique to directly assess the abundance of different fungi in lichens. Twenty-eight lichen thalli were chosen, some with and some without externally visible symptoms of parasite infection, and these were subjected to total DNA extraction. PCR was conducted with fungal-specific primers for the ITS region of ribosomal DNA. Single strands of the products were separated on native acrylamide gels. The majority of lichen specimens, both infected and those without symptoms, displayed more than one band in the stained gels. In one case, 14 bands were detected using SSCP. Some of these bands apparently represent other neighbouring lichens in the habitat, but many are apparently non-lichen-forming. Since few lichen-associated fungi have been cultured and sequenced, it is difficult to know if SSCP bands represent obligate lichenicolous fungi, other asymptomatic lichen parasites, or fungi not obligately associated with lichens, but our results indicate that large numbers of non-lichen-forming fungi commonly co-occur with lichens in nature. For specimens of the filamentous lichens Cystocoleus ebeneus and Racodium rupestre we used cloned sequences to compare the number of sequences obtained by the SSCP method to the number obtained by direct sequencing of thallus extracts, and we generally found that more sequences could be detected by SSCP than could be seen by direct sequencing.

scholarly journals Screening of homokaryotic protoclones of Agaricus bisporus (J. Lge) Imbach by colony characters and ISSR markers

2010 ◽  
Vol 39 (1) ◽  
pp. 119-122 ◽  
Author(s):  
Mahmudul Islam Nazrul ◽  
Fan Xiao Lin ◽  
Bian Yin-Bing
Keyword(s):  
Simple Sequence Repeat ◽  
Agaricus Bisporus ◽  
Morphological Characters ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Fruiting Bodies ◽  
Sequence Repeat ◽  
Slow Growing ◽  
Simple Sequence ◽  
Issr Primers

Among ten slow-growing protoclones of Agaricus bisporus (J. Lge) Imbach, all appressed colonies showed slower growth rate and spawn run, and inability to produce fruiting bodies in substrate. Seven of 40 inter-simple sequence repeat (ISSR) primers amplified 78 reproducible fragments, 48.93% were polymorphic, each producing 7 to 16 bands ranging from 0.10 to 2.10 kbp, sufficient to differentiate the protoclones from each other. Appressed protoclones were homoallelic at a number of loci that were heteroallelic in the parent, suggesting that they represented rare homokaryons. Thus, using morphological characters along with ISSR, polymorphisms could be useful for quick, easy, and accurate in distinguishing homo- and heterokaryotic isolates. Key words: Agaricus bisporus (J. Lge) Imbach; Homokaryon; ISSR; Protoclone DOI: 10.3329/bjb.v39i1.5537Bangladesh J. Bot. 39(1): 119-122, 2010 (June)

scholarly journals Development of specific markers for the detection of Tolypella canadensis eDNA in water samples

2021 ◽  
Vol 4 ◽  
Author(s):  
Christina Wiebe ◽  
Petra Nowak ◽  
Hendrik Schubert
Keyword(s):  
Rare Species ◽  
Environmental Samples ◽  
Large Scale ◽  
Morphological Characters ◽  
Its2 Region ◽  
Specific Marker ◽  
Specific Primers ◽  
Species Specific ◽  
Taxonomic Groups ◽  
Scale Integration

Assessing the biodiversity of an ecosystem plays a major role in ecosystem management. However, proper determination on species-level is often tricky when morphological features are scarce and especially rare species require huge sampling efforts to be detected in the aquatic realm. As an alternative to conventional methods, environmental samples can be examined via the eDNA method, allowing for large-scale integration as well as taxa resolution independent from expression of morphological characters. However, to apply this technique genetic markers that are specific to a species or at least a genus are required. Such markers until now have been successfully developed only for a few well studied taxonomic groups like, e.g., fishes and amphibians, but are still missing for others, especially plants and algae (e.g. Bista et al. 2017). This project focusses on the development of species-specific markers for the macrophytic green algae Tolypella canadensis (Characeae, Charophyta), a rare alga preferring deep water and known so far mainly from remote places. Tolypella canadensis is a circumpolar species and prefers oligotrophic lakes, where it grows in depths up to 13 m (Langangen 2002; Romanov and Kopyrina 2016). In addition, proper determination of Tolypella-species is a field of a few specialists, further complicating monitoring or even detection of this rare species. The design of the species-specific primers was based on reference nucleotide sequences of the chloroplast genes rbcL, psbC and atpB and of the ribosomal internal transcribed spacer regions ITS1 and ITS2, obtained from GenBank (Perez et al. 2017). To determine the specificity of the newly designed primers, DNA isolates obtained from T. canadensis specimens collected from the Torneträsk (Sweden, 2018) and other charophyte species were prepared in different proportions. The sensitivity of the primers was experimentally assayed by using serial dilutions of T. canadensis DNA. Additionally, a mock test comprised of a sample with the DNA of several charophyte species was conducted and finally, the markers were tested on environmental samples from the Torneträsk. Tolypella canadensis-specific primers of the ITS2 region yielded positive PCR amplifications of one single band when T. canadensis was present in a sample. Cross-amplification was not found during the mock test; other charophyte species did not yield positive amplification. The eDNA samples from the Torneträsk validated the performance of the ITS2 marker. The T. canadensis-specific marker designed in this project was proven to be sensitive and accurate. It could be recommended as a useful tool to detect the presence of T. canadensis DNA, even at low concentration and in complex samples containing other charophyte species.

scholarly journals KARAKTERISTIK AKAR BEREKTOMIKORIZA PADA Shorea pinanga, Pinus merkusii DAN Gnetum gnemon

PERENNIAL ◽  
2010 ◽  
Vol 6 (1) ◽  
pp. 11
Author(s):  
Melya Riniarti ◽  
Irdika Mansur ◽  
Arum Sekar Wulandari ◽  
Cecep Kusmana
Keyword(s):  
Key Words ◽  
Ectomycorrhizal Fungi ◽  
Host Plants ◽  
Clamp Connection ◽  
The Other ◽  
Branching Pattern ◽  
Root Tips ◽  
Hartig Net ◽  
Gnetum Gnemon ◽  
Pinus Merkusii

Morphology and anatomy characteristics often used to identify ectomycorrhizal fungi. We used three Scleroderma spp. (Scleroderma columnare, S. dictyosporum), and S. sinnamariense) and inoculated to Shorea pinanga, Pinus merkusii, and Gnetum gnemon. After 6,8, and 10 months, each root tips were collected to determined hyphae colour, branching pattern, clamp-connection, hartig net and mantle. This result revealed that S. sinnamariense did not form association with S. pinanga and P. merkusii but form association with G. gnemon. On the other hand, S. columnare and S. dictyosporum could form association with all the host plants. S. columnare and S. dictyosporum formed white hyphae while S. sinnamariense formed yellow hyphae with monopodial branching pattern. The depth of hartig net and mantle was increased by timed. Key words: ectomycorrhizal fungi, hartig net, mantle, Scleroderma

First report of Hericium cirrhatum from Pakistan

Mycotaxon ◽  
2020 ◽  
Vol 135 (4) ◽  
pp. 845-852
Author(s):  
Junaid Khan ◽  
Hassan Sher ◽  
Shah Hussain ◽  
Abdul Nasir Khalid
Keyword(s):  
Dna Sequence ◽  
Internal Transcribed Spacer ◽  
Morphological Characters ◽  
Sequence Analyses ◽  
Internal Transcribed Spacer Region ◽  
First Report ◽  
First Time

Hericium cirrhatum, a widespread but locally rare tooth fungus is reported for the first time from Pakistan. This species is characterized by white to cream semicircular basidiomata (usually arranged in tiers) with a granular to slightly spiny sterile upper surface, a fertile under surface with 10–15 mm long pointed spines, and ellipsoid to subglobose basidiospores. Morphological characters and DNA sequence analyses inferred from the nuclear ribosomal internal transcribed spacer region (nrITS) confirm the identity of the taxon.

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