Étude de divers paramètres en vue de la culture in vitro des ovules de Capsella bursa-pastoris
Some criteria useful for the analysis of the development of embryos in fertilized ovules cultured in vitro were examined in this preliminary study. At first, it was necessary, in order to know the initial length of the embryo at time of inoculation without opening the ovule, to establish a relation between the size of the ovule and the length of the embryo. The study of the variation of length of the embryo according to the size of ovule in situ is expressed by an exponential curve whose equation is established. The choice of ovules for inoculation was made considering both the graph and the survival of embryos in ovules cultivated in vitro. The 6-day period of culture was determined by examining ovule growth. Study of ovule development, embryo growth and survival led to the choice of an optimal sucrose concentration of 80 g/L.