Production de plantes haploïdes de Gerbera jamesonii par culture in vitro d'ovules

1986 ◽  
Vol 64 (10) ◽  
pp. 2355-2357 ◽  
Author(s):  
Mamar Ahmim ◽  
Joachim Vieth
Keyword(s):  
Acetic Acid ◽  
Indole Acetic Acid ◽  
Gerbera Jamesonii ◽  
Optimal Conditions ◽  
Benzyl Adenine ◽  
Culture In Vitro ◽  
Callus Production ◽  
Unfertilized Ovules ◽  
Haploid Plants

Unfertilized ovules of Gerbera jamesonii were cultivated in vitro and haploid plants were successfully regenerated. Five concentrations of sugar, and four hormonal combinations containing indole acetic acid and benzyl adenine were tested. The best results were obtained with 0.1 mg indole acetic acid/L and 0.2 mg benzyl adenine/L; 1% sugar in the medium was sufficient for callus production and regeneration of plantlets from the clone of the experimental cultivar, 'Super Gerbera'. One hundred and fifty ovules per capitulum can be cultivated and under optimal conditions a frequency of 5% haploid plantlets can be obtained.

scholarly journals In vitro propagation of some tomato (Lycopersicon esculentum Mill.) hybrids

2007 ◽  
Vol 1 (1) ◽  
pp. 52-60
Author(s):  
Kadhum Muhammed Ibrahem ◽  
Hamza Musa Kadhum ◽  
Ali Abadi Manea
Keyword(s):  
Acetic Acid ◽  
Light Intensity ◽  
Lycopersicon Esculentum ◽  
In Vitro Propagation ◽  
Indole Acetic Acid ◽  
Shoot Multiplication ◽  
Ms Medium ◽  
Benzyl Adenine ◽  
Surface Sterilization

Seeds of four tomato hybrids Ginan, Nirtia, Speedy and Rola were cultured on aseptic germination medium after surface sterilization with NaOCl. Shoot tips 1cm length were dissected and grown on MS medium supplemented with Benzyl adenine (BA) and Indole acetic acid (IAA) for multiplication. Callus was induced on hypocotyls cultured on MS medium supplemented with BA under light intensity of 1000 lux and 25 0C. Results showed that NaOCl at 4% completely disinfected seeds and resulted in pathogen free seedlings. Results also showed that a concentration of 4 mg/l of BA was superior in increasing callus fresh and dry weights. Shoot multiplication was achieved on the same medium supplemented with 2 and 0.8 mg/l of BA and IAA respectively.

scholarly journals Effects of different combinations of benzyl adenine and indole acetic acid concentrations on in vitro plant regeneration in hexaploid wheat

2012 ◽  
Vol 3 (2) ◽  
pp. 111-114 ◽  
Author(s):  
Ashraf H. Fahmy ◽  
Jiarui Li ◽  
Walaa Abou El-Wafa ◽  
Soheir El-Saeed El-Khodary ◽  
Osama M. El Shihy
Keyword(s):  
Acetic Acid ◽  
Plant Regeneration ◽  
Hexaploid Wheat ◽  
Indole Acetic Acid ◽  
In Vitro Plant Regeneration ◽  
Benzyl Adenine ◽  
In Vitro Plant

scholarly journals Characterization of Plant Growth-Promoting Traits and Inoculation Effects on Triticum durum of Actinomycetes Isolates under Salt Stress Conditions

Soil Systems ◽  
2021 ◽  
Vol 5 (2) ◽  
pp. 26
Author(s):  
Rihab Djebaili ◽  
Marika Pellegrini ◽  
Massimiliano Rossi ◽  
Cinzia Forni ◽  
Maria Smati ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Salt Stress ◽  
Plant Growth ◽  
Durum Wheat ◽  
Growth And Development ◽  
Indole Acetic Acid ◽  
Greenhouse Experiment ◽  
Hydrocyanic Acid ◽  
In Vitro Assays

This study aimed to characterize the halotolerant capability, in vitro, of selected actinomycetes strains and to evaluate their competence in promoting halo stress tolerance in durum wheat in a greenhouse experiment. Fourteen isolates were tested for phosphate solubilization, indole acetic acid, hydrocyanic acid, and ammonia production under different salt concentrations (i.e., 0, 0.25, 0.5, 0.75, 1, 1.25, and 1.5 M NaCl). The presence of 1-aminocyclopropane-1-carboxylate deaminase activity was also investigated. Salinity tolerance was evaluated in durum wheat through plant growth and development parameters: shoot and root length, dry and ash-free dry weight, and the total chlorophyll content, as well as proline accumulation. In vitro assays have shown that the strains can solubilize inorganic phosphate and produce indole acetic acid, hydrocyanic acid, and ammonia under different salt concentrations. Most of the strains (86%) had 1-aminocyclopropane-1-carboxylate deaminase activity, with significant amounts of α-ketobutyric acid. In the greenhouse experiment, inoculation with actinomycetes strains improved the morpho-biochemical parameters of durum wheat plants, which also recorded significantly higher content of chlorophylls and proline than those uninoculated, both under normal and stressed conditions. Our results suggest that inoculation of halotolerant actinomycetes can mitigate the negative effects of salt stress and allow normal growth and development of durum wheat plants.

Specificity of Phospholipid Binding to Indole Acetic Acid and Other Auxins

1974 ◽  
Vol 29 (1-2) ◽  
pp. 39-41 ◽  
Author(s):  
H. Veen
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Indole Acetic Acid ◽  
Phospholipid Binding ◽  
Primary Action

Abstract Studies in vitro on the binding of phospholipids with IAA, α-NAA and some of their biologically inactive analogues demonstrate that the observed interaction between IA A and lecithin is not related to the primary action of the hormone in plant growth.

scholarly journals Isolation and screening for plant growth-promoting (PGP) actinobacteria from Araucaria angustifolia rhizosphere soil

2010 ◽  
Vol 67 (6) ◽  
pp. 743-746 ◽  
Author(s):  
Rafael Leandro Figueiredo de Vasconcellos ◽  
Mylenne Calciolari Pinheiro da Silva ◽  
Carlos Marcelo Ribeiro ◽  
Elke Jurandy Bran Nogueira Cardoso
Keyword(s):  
Acetic Acid ◽  
Indole Acetic Acid ◽  
Culture Media ◽  
Araucaria Angustifolia ◽  
In Vitro Production ◽  
Soil Ecosystems ◽  
Plant Growth Promoting ◽  
Phosphate Solubilizing ◽  
Vitro Production

Actinobacteria are capable of playing several different roles in soil ecosystems. These microorganisms affect other organisms by producing secondary metabolites and are responsible for the degradation of different complex and relatively recalcitrant organic compounds. In our survey of actinobacteria isolated from the rhizosphere of Araucaria angustifolia, five culture media (AI, WYE, YCED, MSSC and LNMS) were compared for their effectiveness in isolating these microorganisms. When summing up all the isolates randomly obtained, we got 103 isolates. After isolation, the phosphate-solubilizing ability and the "in vitro" production of indole-acetic acid and chitinases were evaluated. The AI medium was ineffective for actinobacteria isolation, when it was compared with the other four culture media. Indole-acetic acid and chitinase were produced by respectively 36% and 24% of the strains tested. However, only 2% of the 103 strains presented some phosphate-solubilizing ability. These results demonstrate the biotechnological potential of these microorganisms.

scholarly journals PENGARUH VARIASI KONSENTRASI INDOLE ACETID ACID (IAA) TERHADAP PERTUMBUHAN TUNAS PISANG BARANGAN (Musa acuminata L. triploid AAA.) DALAM KULTUR IN VITRO

2017 ◽  
Vol 5 (1) ◽  
Author(s):  
Ummi Lathyfah ◽  
Endah Rita Sulistya Dewi
Keyword(s):  
Acetic Acid ◽  
Indole Acetic Acid ◽  
Musa Acuminata

Penelitian ini bertujuan untuk mengetahui pengaruh variasi konsentrasi IndoleAcetid Acid (IAA) terhadap pertumbuhan tunas pisang Barangan (Musaacuminate L. triploid AAA.) serta konsentrasi yang optimum dalam kultur invitro. Penelitian ini dilakukan di Kebun Benih Holtikultura (KBH) KecamatanSalaman, Magelang. Penelitian dilaksanakan selama enam minggu, dimulaiMaret-April 2014. Penelitian menggunakan Rancangan Acak Lengkap (RAL)dengan 4 perlakuan dan 4 kali ulangan yaitu perlakuan 1 (BP1) menggunakan0 ppm IAA, pelakuan 2 (BP2) menggunakan 0,1 ppm IAA, perlakuan 3 (BP3)menggunakan 0,3 ppm IAA, dan perlakuan 4 (BP4) menggunakan 0,6 ppm.Data yang diperoleh dianalisis dengan menggunakan Uji Jarak Ganda Duncan(UJGD). Hasil Analisis Varians menunjukkan konsentrasi paling optimal untukmeningkatkan jumlah anakan tunas 0,3 ppm IAA dan 0,6 ppm untukmeningkatkan jumlah akar, panjang daun, serta panjang batang semu.Kesimpulan dari penelitian ini yaitu variasi konsentrasi Indole Acetic Acid(IAA) secara umum tidak berpengaruh nyata terhadap pertumbuhan pisang Barangan (Musa acuminata L. triploid AAA), meskipun terdapat satuparameter yang berpengaruh nyata, yaitu jumlah anakan tunas pisangBarangan.Kata kunci : Indole Acetic acid (IAA), kultur in vitro, pisang Barangan.

scholarly journals Influence of Indole acetic acid and Tryptophan on production of Vinblastine and Vincristine of Catharanthus roseous callus cells in the accumulation media of In Vitro tissue culture

2010 ◽  
Vol 7 (3) ◽  
pp. 1113-1119
Author(s):  
Baghdad Science Journal
Keyword(s):  
Tissue Culture ◽  
High Performance Liquid Chromatography ◽  
Acetic Acid ◽  
Liquid Chromatography ◽  
In Vitro Culture ◽  
High Performance ◽  
Indole Acetic Acid ◽  
Culture Technique

This study on the plant of Ain –AL Bason Catharanthus roseous showed the ability of callus cells that is produced by In Vitro culture technique and transformed to the accumulated media (MS 40gm/L sucrose ,2gm/L IAA Indole acetic acid , 0.5gm/L Tryptophan) to produce Vinblastine and Vincristine compounds. Extraction, purification and quantitive determination of Vinblastine and Vincristine compounds using High performance liquid chromatography technique (HPLC)were carried out. The results showed that the highest concentration of Vinblastine and Vincristine compounds were ( 4.653,12.5 (ppm /0.5 dry Wight respectively from transformed callus cells from MS 40 gm /L sucrose , 2 gm / L NAA Naphthaline acetic acid .

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