Interspecific and intraspecific variation in pollen morphology in four species of Salix

1990 ◽  
Vol 68 (7) ◽  
pp. 1497-1501 ◽  
Author(s):  
K. H. Kim ◽  
L. Zsuffa ◽  
A. Kenney ◽  
A. Mosseler
Keyword(s):  
Intraspecific Variation ◽  
Pollen Morphology ◽  
Polar Axis ◽  
Interspecific Variation ◽  
Pollen Grain ◽  
Equatorial Diameter ◽  
Specific Variation ◽  
Axis Length ◽  
Interspecific And Intraspecific Variation ◽  
Morphology Parameters

A study of inter- and intra-specific variation in pollen grain polar axis length, pollen equatorial diameter, colpus length, and colpus width in Salix discolor, S. eriocephala, S. lucida, and S. petiolaris demonstrated significant interspecific variation, unequal distances between the species, and various degrees of intraspecific variation. The taxonomic value of the pollen morphology parameters measured was found to vary according to species. A relationship between the pollen parameters measured to the ploidy level of the species is hypothesized. Key words: Salix, pollen, taxonomy.

scholarly journals Pollen Morphology of Six Indonesian Begonia (Begoniaceae) Species

2021 ◽  
pp. 7-13
Author(s):  
Hartutiningsih M. Siregar ◽  
. Sudarmono ◽  
Joko Ridho Witono ◽  
Hary Wawangningrum
Keyword(s):  
Pollen Morphology ◽  
Polar Axis ◽  
Pollen Grain ◽  
Laboratory Research ◽  
Equatorial Diameter ◽  
Fresh Pollen ◽  
Morphological Differences ◽  
Micrometer Scale

Aims: This study aims to determine pollen morphological differences in six Indonesian Begonia species. Study Design: All fresh pollen grain of six Indonesian Begonia species were collected using a standard method and observed at SEM (Scanning Electron Microscope) Laboratory. Place and Duration of Study: The pollen is collected from Bogor Botanic Gardens, then observed using an SEM in The Museum Zoologicum Laboratory, Research Center for Biology, Indonesian Institute of Sciences (LIPI), on August 2018. Methodology: The results of SEM pollen ultrastructure observations were analyzed descriptively by describing images of size, P/E index, aperture, endoaperture, and ornamentation of pollen. The size of pollen morphology is calculated based on the length of the polar axis and the equatorial axis on a micrometer scale. Determination of the shape of pollen can be determined by comparing the length of the polar axis with the equatorial axis. Results: All species examined share common pollen grain characters, i.e. isopolar and three zonocolporate. The longest polar axis (P) and equatorial diameter (E) presented by B. puspitae, whereas the shortest of P is shown by B. kudoensis and the shortest of E by B. sudjanae. Only B. hooveriana has prolate – perprolate aperture, whereas other species perprolate. Endoaperture types of B. puspitae, B. sudjanae, and B. hooveriana is lalongate, while the other species is lolongate. The coarsely striate ornamentation forms are presented by B. holosericeoides and B. natunaensis, whereas other species has fine striate ornamentation. Conclusion: The size, P/E index, aperture, endoaperture, and ornamentation of pollen are not useful for section classification of six Indonesian Begonia species. Pollen morphology should be incorporated to other characters, such as morphological, cytological, and molecular characters for making delimitation of Begonia species.

Scanning electron microscopy observations of pollen morphology in common vetch (Vicia sativa) subspecies

2021 ◽  
pp. 1-14
Author(s):  
Rui Dong ◽  
Zhongjie Lu ◽  
Zhengyu Yang ◽  
Yanrong Wang ◽  
Chao Chen
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Morphology ◽  
Polar View ◽  
Polar Axis ◽  
Vicia Sativa ◽  
Common Vetch ◽  
Plant Phylogeny ◽  
Axis Length ◽  
Scanning Electron

Abstract Common vetch (Vicia sativa) is an important forage and green manure crop that is widely cultivated around the world. However, the large number of subspecies and accessions of common vetch has made taxonomic research on this species difficult. Pollen morphology data can provide important evidence in the study of plant phylogeny. Therefore, in this research, light microscopy and scanning electron microscopy were used to observe seven morphological traits of pollens from 22 common vetch accessions, and residual maximum likelihood and pattern analysis was conducted. The results showed that the pollen grains of the 22 accessions were all monad pollen and the polar view revealed three-lobed circular and triangular shapes, while the equatorial view mainly revealed an oblate shape; only one accession showed an oblate spherical shape. All accessions were 3-zonocolporate and the colpus length extended close to the poles. The polar axis length was (19.39 ± 0.97)–(42.12 ± 0.76) μm and the equatorial axis length was (35.97 ± 1.27)–(45.25 ± 0.81) μm. We found that qualitative traits of pollen shape, aperture polar view and ornamentation were highly stable. Among them, polar axis length, equatorial axis length and colpus length and width had significant differences among the accessions. The ratios of the equatorial and polar axes had significant differences among the subspecies. This trait information could be used for the classification and identification of common vetch accessions and subspecies.

scholarly journals MORFOLOGI POLEN VEGETASI MANGROVE DI DESA SALO PALAI KEC. MUARA BADAK KAB. KUTAI KARTANEGARA

2018 ◽  
Vol 9 (1) ◽  
pp. 40
Author(s):  
Titin Yuliana ◽  
Syafrizal Syafrizal ◽  
Nova Hariani
Keyword(s):  
Pollen Morphology ◽  
Polar Axis ◽  
Equatorial Diameter ◽  
Mangrove Vegetation ◽  
Qualitative Descriptive ◽  
Descriptive Method

This study aims to identified pollen morphology of Mangrove vegetation in Salo Palai Village, Muara Badak Sub District, Kutai Kartanegara District was done in May until July. This study using acetolisis method. Data obtained were analyzed with qualitative descriptive method to observed the shape, ornaments and also aperture. This method is measuring size, the length of the polar axis to the equatorial diameter. The result obtained shape and pollen unit is monad subspheroidal. There are size medium pollen and size large pollen. The ornaments including of 7 psilate, 2 reticulate and 2 ekinate. The aperture including of 4 inaperture, 2 tricolporate, 1 tricolpate, 1 triporate, 1 tetraporate, 1 monoporate and 1 stephanocolporate.Keywords: Vegetation, Mangrove, Pollen, Association, Identification.

scholarly journals Pollen morphology of Malvaceae genera from Saudi Arabia and its taxonomic significance

2021 ◽  
pp. 725-742
Author(s):  
Kadry Abdel khalik ◽  
Suad Al- Ruzayza ◽  
Abdullah Assiri ◽  
Ahmed Elkordy
Keyword(s):  
Saudi Arabia ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Polar Axis ◽  
Morphological Characters ◽  
Average Height ◽  
Prolate Spheroidal ◽  
Oblate Spheroidal ◽  
Equatorial Diameter ◽  
Pollen Shape

Pollen morphology of 20 species belong to seven genera (Abutilon, Althaea, Hibiscus, Malva, Pavonia, Senra and Sida) of Malvaceae from Saudi Arabia were studied by using light microscope (LM) and scanning electron microscope (SEM). Quantitative and qualitative pollen morphological characters which vary among investigated taxa are found in the pollen polarity, symmetry, size, shape, polar axis, equatorial diameter, P/E ratio, average height and width of spine, aperature character and spine index. The pollen grains vary from spheroidal, prolate spheroidal, oblate spheroidal to suboblate. All taxa were characterized by relatively large to medium sized pollen grains, numerous pores scattered irregularly all over the grain, and echinate sculpturing. Sida ovata is the largest size pollen grain (138.95) µm. On the other hand, Malva parviflora showed the smallest pollen size (52.28 µm). The average height and width of spine varied greatly among studied taxa. The highest spines (20.65µm) found in Sida ovata, while the shortest (3.19 µm) was found in Abutilon pannosum. Results of the pollen shape, size, and exine sculpture characters offered useful data for evaluating the taxonomy of Malvaceae both on subgeneric and sectional levels. A key for the identification of the investigated taxa based on pollen grains characters is also provided

scholarly journals Spore morphology of Vaginularia Fée species (Pteridaceae) from South-Eastern Asia A.

2017 ◽  
Vol 7 (4) ◽  
pp. 231-233
Author(s):  
A. V. Vaganov ◽  
I. I. Gureyeva ◽  
A. A. Kuznetsov ◽  
R. S. Romanets
Keyword(s):  
Comparative Study ◽  
Smooth Surface ◽  
Polar Axis ◽  
Spore Morphology ◽  
Scanning Electronic Microscopy ◽  
Eastern Asia ◽  
Electronic Microscopy ◽  
South Eastern ◽  
Equatorial Diameter ◽  
Axis Length

A comparative study of spores of two species of Vaginularia (Vittarioideae, Pteridaceae) from South-Eastern – Vaginularia paradoxa (Fée) Mett. ex Miq. and V. trichoidea Fée – was performed by the method of scanning electronic microscopy (SEM). Spores are tetrahedral, trilete; they are very similar to each other in smooth surface and relatively narrow laesura arms. Differences between species are in shape of spores in proximal position (triangular with narrowly-rounded corners and concave sides in V. paradoxa and triangular with broadly-rounded corners and slightly concave sides in V. trichoidea), in length of laesura arms relative to the radius of the spore (laesura arms are about 0.75 of spore radius in V. paradoxa, and riches corners of spore in V. trichoidea) and in size of spore (spore of V. paradoxa are about 1.5 times more in equatorial diameter and 1.7 times more in polar axis length).

scholarly journals Pollen Morphology of Herbaceous Peonies with Different Ploidy Levels

2016 ◽  
Vol 141 (3) ◽  
pp. 275-284 ◽  
Author(s):  
Lihong Hao ◽  
Hui Ma ◽  
Jaime A. Teixeira da Silva ◽  
XiaoNan Yu
Keyword(s):  
Pollen Morphology ◽  
Pollen Grains ◽  
Polar View ◽  
Polar Axis ◽  
Hybrid Breeding ◽  
Ploidy Levels ◽  
Herbaceous Peony ◽  
Group I ◽  
Surface Ornamentation ◽  
Axis Length

The pollen morphology of 26 cultivars of herbaceous peony (Paeonia L.) was examined by scanning electron microscopy (SEM). Pollen grains of the cultivars were mostly subprolate to perprolate, tricolporate, or ellipsoidal in equatorial view, but rounded-trifid in polar view. The poles of pollen grains were mainly flat when viewed in cross-section and only a few appeared to have a circular or arc shape. In addition, the surface of the pollen grains was psilate, and the muri were partly or fully protuberant with a reticulate surface sculpture. However, the shapes and sizes of pollen grains as well as surface ornamentation varied significantly among cultivars with different chromosome numbers. The pollen grains from diploid cultivars were regular and full, and the reticulation holes were evenly distributed, while most of the pollen grains from triploid cultivars were empty flat shells with some heteromorphic pollen whose surface ornamentation was mostly rugulate-reticulate exine. The pollen grains from tetraploid cultivars were the largest although pollen morphology and surface ornamentation differed between Athena and Cream Delight. Six indices [polar axis length (P), equator axis length (E), P/E, pollen perforation diameter (D), ridge width (W), and D/W] were used in Q cluster analysis, which divided the 26 cultivars into three groups. Group I included eight diploid, four triploid, and one tetraploid cultivar. Group II included 12 triploid cultivars with complex genetic backgrounds. Group III included only one tetraploid cultivar Cream Delight. This work may provide an important palynological basis for studying taxonomy and hybrid breeding of herbaceous peony cultivars.

scholarly journals Morphology of pollen grains of 25 species of Salix (Salicaceae) of the Asian part of Russia according to electron microscopy

2019 ◽  
pp. 94-101 ◽  
Author(s):  
A. A. Petruk
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
Pollen Grains ◽  
Grain Morphology ◽  
Polar Axis ◽  
Pollen Grain ◽  
Equatorial Diameter ◽  
Different Shapes ◽  
Pollen Grain Morphology ◽  
Scanning Electron

This is a study of pollen grain morphology of 25 species of the genus Salix L. (Salicaceae) from of the Asian part of Russia was carried out with the use of scanning electron microscope. All species had 3-colpate pollen grains. The length of the polar axis varied from 14 to 30 mkm, the length of the equatorial diameter - from 9.5 to 17.4 mkm. This study has shown the different shapes of the pollen grains, such as the elliptic, broadly and narrowly elliptic.

Pollen morphology of some Verbascum (Scrophulariaceae) taxa in Turkey

Phytotaxa ◽  
2018 ◽  
Vol 333 (2) ◽  
pp. 209 ◽  
Author(s):  
AYŞE ÖZTÜRK ◽  
KUDRET BETÜL GÜNEY ◽  
BARIŞ BANİ ◽  
KERİM GÜNEY ◽  
FAİK AHMET KARAVELİOĞULLARI ◽  
...  
Keyword(s):  
Pollen Morphology ◽  
Pollen Grains ◽  
Polar Axis ◽  
Prolate Spheroidal ◽  
Oblate Spheroidal ◽  
General Terms ◽  
Surface Ornamentation ◽  
Pollen Shape ◽  
Axis Length ◽  
Scanning Electron

Pollen morphology of six Verbascum (Scrophulariaceae) taxa belonging to five different groups in the genus [V. georgicum (group E), V. nudatum var. nudatum (group F), V. cariense (group F), V. bithynicum (group H), V. pycnostachyum (group K), and V. lasianthum (group L)] was studied. Pollen slides were prepared according to the Woodhouse method and were examined with light-microscopy (LM) and Scanning Electron Microscopy (SEM). All studied pollen grains are generally monads, though tetrads (5 %) were observed in Verbascum nudatum var. nudatum. Pollen grains of all studied taxa were determined as isopolar and radially symmetric. In general terms, aperture types are trizonocolporate, but also trisyncolpate (V. lasianthum 10 %, V. pycnostachum 1 %, and V. georgicum 4 %) and trizonocolpate (V. cariense 10 %, V. bithynicum 10%, V. lasianthum 90%, V. pycnostachyum 9%, and V. georgicum 96 %) aperture types were observed. The shapes of the pollen grains were determined as prolate, subprolate, prolate-spheroidal and oblate-spheroidal. The sculpture was reticulate in all cases. The results of the study corroborate that morphological features, such as pollen shape, polar axis length (P), equatorial axis length (L), aperture type, surface ornamentation, muri thickness, and lumina shape, show differences which can be taken into consideration in the systematic discrimination within the groups, and also for discrimination of the groups in the genus.

scholarly journals Pollen morphology and variability of Polish native species from genus Salix L.

PLoS ONE ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. e0243993
Author(s):  
Irmina Maciejewska-Rutkowska ◽  
Jan Bocianowski ◽  
Dorota Wrońska-Pilarek
Keyword(s):  
Native Species ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Polar Axis ◽  
High Variability ◽  
Interspecific Variability ◽  
Equatorial Diameter ◽  
Exine Ornamentation ◽  
Lower Variability ◽  
Biometric Features

The pollen morphology was studied of 24 Salix species native to Poland, which represented two subgenera, 17 sections and five subsections occurring in Poland. The aim of this study was to discover the taxonomical usefulness of the pollen features under analysis, and to investigate the ranges of their interspecific variability. In total, 720 pollen grains were studied. They were analysed with respect to seven quantitative features (length of the polar axis ˗ P, equatorial diameter ˗ E, length of the ectoaperture ˗ Le, exine thickness ˗ Ex, and P/E, Ex/P and Le/P ratios) and the following qualitative ones: pollen outline and exine ornamentation. The most important features were exine ornamentation (muri, lumina and margo) characters. The pollen features should be treated as auxiliary because they allowed to distinguish eight individual Salix species, and five groups of species. Statistical analysis of the studied traits indicated a high variability among the tested species. The most variable biometric features were P, E and Le, while lower variability occurred in P/E, Le/P and d/E.

scholarly journals Pollen morphology of Rosa gallica L. (Rosaceae) from Southern Poland

2011 ◽  
Vol 74 (4) ◽  
pp. 297-304 ◽  
Author(s):  
Dorota Wrońska-Pilarek ◽  
Krystyna Boratyńska
Keyword(s):  
Pollen Morphology ◽  
Pollen Grains ◽  
Polar Axis ◽  
Pollen Grain ◽  
The Other ◽  
Diagnostic Features ◽  
Linear Arrangement ◽  
Pleistocene Refugia ◽  
Southern Poland ◽  
Rosa Gallica

The morphology of pollen grains of <em>Rosa gallica</em> was studied on the basis of material from 15 natural localities in southern Poland. It was ascertained that the diagnostic features of pollen grains of <em>R. gallica</em> were: the presence of deep, often Y-shaped striae in a linear arrangement; numerous perforations of various, rather big diameters on the bottom of striae; the occurrence of operculum on the membrane of ectocolpus; the presence of costae ectocolpi and fastigium in the area of endopori. The most significant differences among the particular samples studied concern the shape of grains. The differences between pollen grains of 15 individuals of <em>Rosa gallica</em> from southern Poland are generally slight. The most discriminating measured character is length of equatorial axis of pollen grain and length of ectocolpi and thickness of exine along polar axis. The remaining differentiate the individuals with smaller significance. The differences between Silesian samples and all the other examined ones may result from origin of different Pleistocene refugia.

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