Pollen morphology of Rosa gallica L. (Rosaceae) from Southern Poland

The morphology of pollen grains of Rosa gallica was studied on the basis of material from 15 natural localities in southern Poland. It was ascertained that the diagnostic features of pollen grains of R. gallica were: the presence of deep, often Y-shaped striae in a linear arrangement; numerous perforations of various, rather big diameters on the bottom of striae; the occurrence of operculum on the membrane of ectocolpus; the presence of costae ectocolpi and fastigium in the area of endopori. The most significant differences among the particular samples studied concern the shape of grains. The differences between pollen grains of 15 individuals of Rosa gallica from southern Poland are generally slight. The most discriminating measured character is length of equatorial axis of pollen grain and length of ectocolpi and thickness of exine along polar axis. The remaining differentiate the individuals with smaller significance. The differences between Silesian samples and all the other examined ones may result from origin of different Pleistocene refugia.

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Pollen Morphology and Variability of Abies alba Mill. Genotypes from South-Western Poland

Forests ◽

10.3390/f11111125 ◽

2020 ◽

Vol 11 (11) ◽

pp. 1125

Author(s):

Dorota Wrońska-Pilarek ◽

Monika Dering ◽

Jan Bocianowski ◽

Kacper Lechowicz ◽

Wojciech Kowalkowski ◽

...

Keyword(s):

Pollen Morphology ◽

Geographical Location ◽

Abies Alba ◽

Intraspecific Variability ◽

Pollen Grains ◽

Polar Axis ◽

Seed Orchard ◽

Diagnostic Features ◽

Pollen Shape ◽

First Time

The objective of this study was to investigate pollen morphology and ranges of intraspecific variability of Abies alba Mill. Pollen grains were collected from nine clonal seed orchards of A. alba in the Sudety Mountains, (South-Western Poland). At each seed orchard, 4–6 grafts were selected. Each individual (graft) was represented by 30 pollen grains and 1440 pollen grains were measured totally. Eight quantitative and four qualitative features of pollen grains were analysed. The diagnostic features of pollen grains for the studied species were: Exine surface of pollen corpus (cappa and leptoma) and sacci, the length of the polar axis (P), pollen shape (P/E ratio), and a new trait—saccus shape (A/B ratio — saccus width (A) to his length (B)). Pollen features made possible to differentiate seven individual genotypes (samples). To our knowledge, this is the first time that the intraspecific and interindividual variability of pollen grains of A. alba were investigated. The most different were the pollen grains from samples—genotypes 13 (Bystrzyca Kłodzka) and 18 (Jugów), and also (although to a lesser extent) genotypes—11 (Kamienna Góra), 30, 31 (Jugów), and 44 (Szklarska Poręba). No significant relationships were observed between the pollen grain traits and the geographical location of the collection sites.

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Pollen morphology of Polish native species of the Rosa genus (Rosaceae) and its relation to systematics

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.2011.031 ◽

2011 ◽

Vol 80 (3) ◽

pp. 221-232 ◽

Cited By ~ 7

Author(s):

Dorota Wrońska-Pilarek

Keyword(s):

Native Species ◽

Pollen Morphology ◽

Pollen Grains ◽

Pollen Grain ◽

Morphological Features ◽

Diagnostic Features ◽

Qualitative Traits ◽

Outline Shape ◽

Analytical Results ◽

Exine Sculpturing

The morphology of pollen grains of 16 species from the Rosa L. genus were studied (i.e. R. agrestis, R. canina, R. dumalis, R. gallica, R. inodora, R. jundzillii, R. kostrakiewiczii, R. majalis, R. micrantha, R. mollis, R. pendulina, R. rubiginosa, R. sherardii, R. tomentosa, R. villosa, and R. zalana). The material came from 16 native localities of those species in Poland. The measurements are based on at least 30-50 randomly selected, fully developed pollen grains per specimen. In total, 500 pollen grains were examined. They were analysed for 13 quantitative features of pollen grains and exine sculpturing and the following qualitative traits: outline, shape, "operculum" structure. The diagnostic features of pollen grains of studied species were: length of polar and equatorial axes and length of ectocolpi. The above-mentioned pollen grain morphological features make isolation of one species possible: R. gallica. R. gallica is distinguished for its highest values of the length of polar and equatorial axes, and the length of ectocolpi. The obtained analytical results of operculum and exine sculpture features, considered as diagnostic, corroborated only slightly their priority significance for the isolation of the examined species and sections. The collected data failed to confirm fully the current taxonomical division of the Rosa genus into sections (only section Gallicanae from R. gallica is isolated) as well as consanguinity relationships between the examined species from the Caninae section. On the dendrogram, both species closely related with each other as well as those from other developmental lines were found in the same group. These equivocal results are by no means surprising because the Caninae section is the most polymorphic group in the Rosa genus, and contemporary Caninae are of the nature of a swarm of R. canina hybrids as a link combining all taxons of the section.

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Pollen morphology of Polish species from the genus Rubus L. (Rosaceae) and its systematic importance

10.1101/734202 ◽

2019 ◽

Author(s):

Kacper Lechowicz ◽

Dorota Wrońska-Pilarek ◽

Jan Bocianowski ◽

Tomasz Maliński

Keyword(s):

Pollen Morphology ◽

Pollen Grains ◽

Polar Axis ◽

The Other ◽

Individual Species ◽

Bridge Structure ◽

Interspecific Variability ◽

Exine Ornamentation ◽

First Time ◽

Systematic Importance

AbstractThe genus Rubus L. (Rosaceae) has as yet not been investigated satisfactorily in terms of palynology. This genus is taxonomically very difficult due to the large number of species and problems with their delimitation, as well as very different distribution areas of particular species. The aim of this study was to investigate pollen morphology and for the first time the ranges of intrageneric and interspecific variability of Rubus species, as well as verify the taxonomic usefulness of these traits in distinguishing studied taxa from this genus. They were analysed for 11 quantitative pollen characteristics and the following qualitative ones: exine ornamentation, pollen outline and shape, as well as bridge structure. Analyses were conducted on a total of 1740 pollen grains, which represent 58 blackberry species belonging to a majority of subgenera and all the sections and series found in Poland. The diagnostic characters included exine ornamentation (exine ornamentation type, width and direction of grooves and striae, number and diameter of perforations) and length of the polar axis (P). The arrangement of the examined species on the dendrogram does not corroborate division of the genus Rubus into subgenera, sections and series currently adopted in taxonomy. The lack of dependence may result from apomixis observed in Rubus, which could reduce natural variability. Pollen features should be treated in taxonomy as auxiliary, because they fail to differentiate several (10) individual species, while the other ones create groups with similar pollen traits.

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Pollen characters as taxonomic evidence in some species of Dipsacaceae from Iran

Bangladesh Journal of Plant Taxonomy ◽

10.3329/bjpt.v24i2.35108 ◽

2017 ◽

Vol 24 (2) ◽

pp. 129-136 ◽

Cited By ~ 1

Author(s):

Ebadi-Nahari Mostafa ◽

Nikzat-Siahkolaee Sedigheh ◽

Eftekharian Rosa

Keyword(s):

Pollen Morphology ◽

Pollen Grains ◽

Pollen Grain ◽

Pollen Type ◽

Upgma Tree ◽

Pollen Characters ◽

Exine Ornamentation ◽

The Family ◽

The World ◽

Plant Taxon

Pollen morphology of nine species representing four genera: Cephalaria Schrad, Dipsacus L., Pterocephalus Vaill. and Scabiosa L. of the family Dipsacaceae in Iran has been investigated by means of scanning electron microscopy (SEM). The results showed that pollen grains were triporate and tricolpate. The pollen type of Scabiosa rotata Bieb. (tri- and tetraporate) is the first report in the world. The sizes of pollen grains fall into the classification group magna (pollen grain diameter 50–100 μm). Pollen shapes vary from preoblate to prolate and their polar views were triangulate and lobate. The exine ornamentation varies from gemmate in S. rotata to spinulate in the rest studied species. Species of Scabiosa have been dispersed in UPGMA tree that this confirmed the previous studies about taxonomic problems and species complexity in this genus. These results show the transfer of the some Scabisoa species to Lomelosia Raf. based on palynological characters. Pollen morphology of the family is helpful at the generic and specific level.Bangladesh J. Plant Taxon. 24(2): 129–136, 2017 (December)

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Pollen morphology of the Polish species of the genus Ribes L.

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.1998.036 ◽

2014 ◽

Vol 67 (3-4) ◽

pp. 275-285 ◽

Cited By ~ 7

Author(s):

Dorota Wrońska-Pilarek

Keyword(s):

Native Species ◽

Pollen Morphology ◽

Pollen Grains ◽

Morphological Characters ◽

Prolate Spheroidal ◽

Diagnostic Features ◽

Grain Diameter

This paper presents results of studies on pollen morphology of 6 Polish species of the genus Ribes L. (family Grossulariaceae). Observations were carried out with LM and SEM. Pollen diagnoses were illustrated by series of microphotographs. Grains of examined species are small to medium-sized and prolate spheroidal or subprolate in shape, rarely prolate. They represent three pollen classes: zonocolporate, pantoaperturate and pantoporate. Three types of ectoapertures have been observed: colpi, pori and colpoids. Endoapertures are pori, in number (4-) 6-7-8 (-14) per grain. Ornamentation is psilate or fossulate, with the exception of the ectoaperture areas, which are granulate. According to the author, the polarity or apolarity, type of pollen class and ectoaperture, number of endopori and ratio of exine thickness to grain diameter are the main diagnostic features of the pollen grains of the native species of Ribes. The key to Polish species, basing on the morphological characters of grains is given too.

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Pollen morphology of the Balkan-Carpathian endemic Campanula lingulata Waldst. & Kit. (Campanulaceae)

Zbornik Matice srpske za prirodne nauke ◽

10.2298/zmspn1630075m ◽

2016 ◽

pp. 75-82

Author(s):

Marina Macukanovic-Jocic ◽

Snezana Jaric

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Light Microscopy ◽

Endemic Species ◽

Pollen Morphology ◽

Average Length ◽

Pollen Grains ◽

Polar Axis ◽

First Time ◽

Scanning Electron

Palynomorphological characteristics of Campanula lingulata, the Balkan-Carpathian endemic species growing in Serbia, have been investigated using light microscopy and scanning electron microscopy for the first time, in order to provide some information helpful for a better understanding of the taxonomic position of this species within the genus, as well as to contribute to the pollen atlas of Serbian apiflora. The pollen grains are radially symmetrical, isopolar, 3-zonoporate and medium-sized monads oblate-sphaeroidal in shape. Mean of the polar axis (P) is 27.6?1.9 ?m, while the average length of the equatorial axis (E) is 28.8?1.6 ?m. The apertures are operculate. The sculpturing pattern of the exine is microre?ticulate-microechinatae. The exine surface is covered with evenly distributed supratectal spinules of variable length and sparse granules. The longest supratectal spinules are 0.64?0.05 ?m in length and the smallest sculptural elements are less than 0.2 ?m high. The microechinae density per sample area of 5 ?m x 5 ?m averages 17.4?2.4.

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Pollen morphology of Sabinaria magnifica (Cryosophileae, Coryphoideae, Arecaceae)

Phytotaxa ◽

10.11646/phytotaxa.207.1.9 ◽

2015 ◽

Vol 207 (1) ◽

pp. 135 ◽

Cited By ~ 1

Author(s):

Giovanni Raul Bogota ◽

Carina Hoorn ◽

Wim Star ◽

Rob Langelaan ◽

Hannah Banks ◽

...

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Transmission Electron Microscopy ◽

Light Microscopy ◽

Pollen Morphology ◽

Pollen Grains ◽

The Other ◽

Transmission Electron ◽

Palm Species ◽

Scanning Electron

Sabinaria magnifica is so far the only known species in the recently discovered tropical palm genus Sabinaria (Arecaceae). Here we present a complete description of the pollen morphology of this palm species based on light microscopy (LM), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). We also made SEM-based comparisons of Sabinaria with other genera within the tribe Cryosophileae. Pollen grains of Sabinaria magnifica resemble the other genera in the heteropolar, slightly asymmetric monads, and the monosulcate and tectate exine with perforate surface. Nevertheless, there are some clear differences with Thrinax, Chelyocarpus and Cryosophila in terms of aperture and exine. S. magnifica differs from its closest relative, Itaya amicorum, in the exine structure. This study shows that a combination of microscope techniques is essential for the identification of different genera within the Cryosophileae and may also be a necessary when working with other palynologically less distinct palm genera.

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Palynology of the Genus Stachytarpheta Vahl. (Verbenaceae)

Notulae Scientia Biologicae ◽

10.15835/nsb244816 ◽

2010 ◽

Vol 2 (4) ◽

pp. 27-33

Author(s):

Olubukola ADEDEJI

Keyword(s):

Cluster Analysis ◽

Grain Size ◽

Evolutionary History ◽

Pollen Grains ◽

Pollen Grain ◽

The Other ◽

Pollen Grain Size ◽

Pollen Diameter ◽

Stachytarpheta Cayennensis ◽

Exine Morphology

The exine morphology of pollen grains of Stachytarpheta indica (Linn.) Vahl, Stachytarpheta cayennensis (Rich.) Vahl and Stachytarpheta angustifolia (Mill.) Vahl is reported. This study was carried out with a light microscope. Pollen grains from fresh anthers were collected and aceolysed. Statistical analysis used to analyse the data collected include cluster analysis, correlation analysis, similarity and distance indices. The pollen grains are spheroidal to oblate to sub-oblate in shape. They are aperturate, both colpate and porate. Tricolpate types occur most frequently, acolpate, monocolpate, bicolpate and tetracolpate types less frequently. The multicolpate and multiporate attributes in all the species indicate that the genus is not primitive in evolutionary history and this species probably, evolved around in the same time. According to the size, the pollen grains of the genus falls into groups permagna (pollen diameter 100-200 μm) and giganta (pollen diameter greater than 200 μm). S. cayennensis and S. anguistifolia belong to group permagna and S. indica only in the group giganta. This separates S. indica from the other two species. The large pollen grain size in the genus clearly supports the fact that the flowers in the genus are more insect-and-bird pollinated than wind pollinated. The similarity and distance indices of the species showed that S. cayennensis and S. angustifolia are the closest. S. indica is closer to S. angustifolia but farther from S. cayennensis.

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Pollen morphology of the Rosaceae of western Canada. IV. Luetkea, Oemleria, Physocarpus, Prunus

Canadian Journal of Botany ◽

10.1139/b91-322 ◽

1991 ◽

Vol 69 (12) ◽

pp. 2583-2596 ◽

Cited By ~ 13

Author(s):

R. J. Hebda ◽

C. C. Chinnappa ◽

B. M. Smith

Keyword(s):

Pollen Morphology ◽

Pollen Grains ◽

Pollen Grain ◽

Western Canada ◽

Prunus Virginiana ◽

Prunus Spinosa ◽

Pore Area ◽

Pollen Grain Size ◽

Triple Points ◽

Scanning Electron

Pollen grains of 12 species of western Canadian rosaceous genera, Luetkea, Oemleria, Physocarpus, and Prunus, were studied in the light microscope and scanning electron microscope. All pollen grains are produced as isopolar, radially symmetrical, usually tricolporate monads. Grains are predominantly spheroidal with a circular to triangular amb. Most of the species exhibit a well-developed chambered aperture complex at the equator, formed by a pair of sexinal flaps whereas in Prunus spinosa, Prunus americana, and Prunus virginiana the chamber is absent or weakly developed. Oemleria cerasiformis has no pore flaps. Apertures of Prunus emarginata and Prunus virginiana often have an equatorial bridge over the aperture complex. The exine of Prunus, Physocarpus, and Luetkea pollen is tectate perforate, like that of many Rosaceae. Sculpturing consists of ridges and valleys that form a striate to rugulate pattern. Ridges and valleys tend to be mainly parallel to the colpus but often loop near the poles. Pollen grains of Prunus spinosa are always rugulate or vermiculate with ridges arranged randomly. Oemleria cerasiformis pollen appears tectate imperforate. Ridges and valleys intersect at triple points in the subpolar region and curve in a semicircle around the pore area. On this basis Oemleria pollen is distinct from all other western Canadian Rosaceae. Pollen grain size ranges from small for Luetkea pectinata (17 μm in diameter) to medium for Prunus spinosa (36 × 33 μm). Key words: pollen morphology, Rosaceae, Western Canada, systematics.

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Pollen morphology of Carapa species (Meliaceae) from the Brazilian Amazon

Acta Amazonica ◽

10.1590/1809-4392201505964 ◽

2016 ◽

Vol 46 (3) ◽

pp. 333-336

Author(s):

Alexsandro ZIDKO ◽

Luiz Antônio RODRIGUES ◽

Cláudia Barbieri Ferreira MENDONÇA ◽

Maria Lucia ABSY ◽

Marcos Gonçalves FERREIRA ◽

...

Keyword(s):

Pollen Morphology ◽

Pollen Grains ◽

Polar View ◽

Brazilian Amazon ◽

Microscopy Study ◽

The Other ◽

Carapa Guianensis ◽

Equatorial Diameter ◽

Taxonomic Implications ◽

Scanning Electron

Abstract The crabwood (Carapa) represents an important source of natural resources. The objective of this work was to study the pollen morphology of the three species of Carapa (Meliaceae) registered in the Brazilian Amazon. The pollen grains were obtained from floral buds, pistillate and staminate flowers of Carapa guianensis, C. surinamensis and C. vasquezii. In order to explore the taxonomic implications of the pollen study for these species of Carapa, lactic acetolysis method was used for light microscopy study. Furthermore, the non-acetolyzed material was used in the study of the scanning electron microscopy. It was found that the three Carapa species present little variations in pollen morphology. Compared to the other species this study, Carapa vasquezii has higher values for the length and width of endoaperture; however, it has lower values for polar and equatorial diameter, equatorial diameter in polar view and apocolpium side.

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