scholarly journals The Effect of Intermittent Exercise on Carbohydrate Metabolism in Rainbow Trout, Salmo gairdneri

1966 ◽  
Vol 23 (4) ◽  
pp. 471-485 ◽  
Author(s):  
E. Don Stevens ◽  
Edgar C. Black
Keyword(s):  
Rainbow Trout ◽  
Carbohydrate Metabolism ◽  
Blood Lactate ◽  
Short Duration ◽  
Muscle Glycogen ◽  
Intermittent Exercise ◽  
Liver Glycogen ◽  
Salmo Gairdneri ◽  
Muscle Lactate ◽  
Severe Exercise

The effect of intermittent severe exercise of short duration on carbohydrate metabolism was examined using unanesthetized, intact rainbow trout. The levels of muscle glycogen, muscle lactate, blood lactate, and liver glycogen were determined in fish sampled immediately after severe exercise of 3 sec to 5 min, after recovery of 3 min to 60 min, and after re-exercise of 3 sec to 5 min. It appears that rainbow trout are not well adapted to tolerate frequent exercise of short duration.

Changes in Glycogen, Pyruvate and Lactate in Rainbow Trout Salmo gairdneri) During and Following Muscular Activity

1962 ◽  
Vol 19 (3) ◽  
pp. 409-436 ◽  
Author(s):  
Edgar C. Black ◽  
Anne Robertson Connor ◽  
Kwok-Cheung Lam ◽  
Wing-Gay Chiu
Keyword(s):  
Rainbow Trout ◽  
Water Content ◽  
Muscle Glycogen ◽  
Whole Blood ◽  
Muscular Activity ◽  
Liver Glycogen ◽  
Salmo Gairdneri ◽  
Moderate Activity ◽  
Experimental Conditions ◽  
Severe Exercise

Experiments upon muscular fatigue in [Formula: see text]-year-old rainbow trout (Salmo gairdneri) were carried out at the trout hatchery at Summerland, British Columbia during the summers of 1958 and 1959. Observations were made on hemoglobin, water content of muscle and whole blood, muscle and liver glycogen, and pyruvate and lactate in muscle, blood and liver. Experimental conditions included 30 min moderate activity, 2–15 min severe exercise, and recovery up to 24 hr following 15 min severe exercise. The results for hemoglobin, muscle and liver glycogen and blood lactate were similar to those reported earlier. Muscle glycogen was depleted 50% or more in the first 2 min of severe activity. Correlated with this rapid depletion of glycogen was a sudden accumulation of muscle pyruvate and lactate. Pyruvate and lactate likewise increased in the blood. While the disappearance of both pyruvate and lactate from muscle began immediately upon cessation of activity, resting levels were not approached until the 8th hr of recovery. During recovery, the levels of pyruvate and lactate in the blood after severe exercise continued to increase for the first hour, remained elevated for at least 8 hr and did not return to the resting level until the 12th–24th hr. Muscle glycogen was not restored above half the resting level at the end of 24 hr. Interpretations of the data in relation to metabolism in rainbow trout are discussed.

Alterations in Glycogen, Glucose and Lactate in Rainbow and Kamloops Trout, Salmo gairdneri, Following Muscular Activity

1960 ◽  
Vol 17 (4) ◽  
pp. 487-500 ◽  
Author(s):  
Edgar C. Black ◽  
Anne C. Robertson ◽  
Arthur R. Hanslip ◽  
Wing-Gay Chiu
Keyword(s):  
Blood Lactate ◽  
Muscle Glycogen ◽  
Muscular Activity ◽  
Liver Glycogen ◽  
Strenuous Exercise ◽  
Salmo Gairdneri ◽  
Moderate Activity ◽  
Starvation Period ◽  
Exercise Muscle ◽  
Lactate Levels

Rainbow trout [Formula: see text] years old (fall spawners) raised in the hatchery at Summerland, B.C., and 2-year-old mature spawning Kamloops trout (spring spawners) captured from Lake Okanagan, were subjected to 15 minutes strenuous exercise. Muscle glycogen was depleted in both groups. Following 30 minutes of moderate activity, muscle glycogen remained high in the [Formula: see text]-year-old trout. Liver glycogen levels were not significantly lowered during either strenuous or moderate exercise. Blood lactate levels were markedly elevated during 15 min of strenuous exercise and continued to rise for 2 hours of post-exercise recovery in both groups of fish. In the [Formula: see text]-year-old trout, blood lactate declined to resting levels at about the 8th hour of recovery, and was increased 3-fold following 30 min of moderate activity. Blood glucose and hemoglobin were not significantly altered during either strenuous or moderate activity.In the [Formula: see text]-year-old trout, starvation of up to 7 days duration resulted in a marked depletion of liver glycogen. There was little change in muscle glycogen, blood lactate, glucose or hemoglobin, regardless of whether or not the fish had been exercised at the beginning of the starvation period. Feeding during the period of recovery from 15 min of strenuous exercise resulted in increases in both muscle and liver glycogen levels.

Changes in Glycogen and Lactate Levels in Migrating Salmonid Fishes Ascending Experimental "Endless" Fishways

1964 ◽  
Vol 21 (2) ◽  
pp. 255-290 ◽  
Author(s):  
Anne R. Connor ◽  
Carl H. Elling ◽  
Edgar C. Black ◽  
Gerald B. Collins ◽  
Joseph R. Gauley ◽  
...  
Keyword(s):  
Muscle Glycogen ◽  
Sockeye Salmon ◽  
Oncorhynchus Tshawytscha ◽  
Liver Glycogen ◽  
Individual Performance ◽  
Control Fish ◽  
Salmo Gairdneri ◽  
Upstream Migration ◽  
Salmonid Fishes ◽  
Muscle Lactate

Certain aspects of the performance and biochemistry of adult migrating salmonid fishes were investigated during ascents of 1:16- and 1:8-slope experimental fishways which employed locking devices permitting the simulation of fishways of any length. Fish tested were chinook salmon (Oncorhynchus tshawytscha), sockeye salmon (O. nerka) and steelhead trout (Salmo gairdneri) diverted from their upstream migration on the Columbia river at the Bonneville dam fishways during the summer of 1959. Blood and muscle lactate, and muscle and liver glycogen levels were determined in control fish, and in fish following three types of activity. Records of individual performance were kept.All species were willing to enter and capable of ascending fishways of either slope up to 1000 ft in height under favourable hydraulic conditions. Blood lactate and muscle glycogen determinations revealed the degree of exercise to be moderate even during prolonged ascents. Moderately high ascents (about 100 ft) in the steeper fishway apparently required the expenditure of some muscle glycogen whereas similar ascents in the 1:16 fishway did not. When muscle glycogen was utilized during prolonged ascents the expenditure appeared to be progressive. All species were apparently able to adapt to prolonged ascents of either fishway. Certain fish of each species tended to stop moving of their own volition in both fishways. After a 60-min volitional stop some evidence of recovery from the effects of exercise was observed. Discussion of the above data is presented.

Catecholamine and Carbohydrate Concentrations in Rainbow Trout (Salmo gairdneri) in Relation to Physical Disturbance

1967 ◽  
Vol 24 (8) ◽  
pp. 1701-1715 ◽  
Author(s):  
T. Nakano ◽  
N. Tomlinson
Keyword(s):  
Skeletal Muscle ◽  
Rainbow Trout ◽  
Blood Plasma ◽  
Muscle Glycogen ◽  
Plasma Concentrations ◽  
Liver Glycogen ◽  
Salmo Gairdneri ◽  
Maximum Plasma ◽  
Physical Disturbance ◽  
High Liver

In rainbow trout (Salmo gairdneri) blood plasma concentrations of adrenaline (AD) and noradrenaline (NAD), and liver and heart concentrations of AD increased in response to severe physical disturbance. Skeletal muscle and anterior kidney concentrations of AD and NAD did not change detectably. Maximum plasma concentrations observed during disturbance of the fish were 0.20–0.36 μg AD and 0.05–0.09 μg NAD/ml. These plasma concentrations decreased relatively rapidly during recovery of the fish.Plasma glucose concentrations increased in response to disturbance, the magnitude and duration of the hyperglycemia being greater in those fish with initially high liver glycogen reserves. Liver glycogen concentrations in those fish with initially high (ca. 7%) concentrations apparently decreased in response to disturbance and increased during recovery of the fish, but no change was detected in liver glycogen concentrations in fish in which they were initially low (ca. 2.5%). Heart and skeletal muscle glycogen concentrations decreased in response to disturbance and increased during recovery.In skeletal muscle, the concentration of adenosine 3′,5′-phosphate and the proportion of phosphorylase in the a form increased in response to disturbance of the fish and decreased thereafter.

Effect of a Partial Loss of Scales and Mucous on Carbohydrate Metabolism in Rainbow Trout (Salmo gairdneri)

1967 ◽  
Vol 24 (5) ◽  
pp. 939-953 ◽  
Author(s):  
Edgar C. Black ◽  
S. J. Tredwell
Keyword(s):  
Rainbow Trout ◽  
Blood Glucose ◽  
Carbohydrate Metabolism ◽  
Liver Glycogen ◽  
Salmo Gairdneri ◽  
Red Cell ◽  
Partial Loss ◽  
Fourfold Increase ◽  
Control Value ◽  
Cell Volumes

This study was undertaken to determine what part partial scaling and partial desliming might play in the survival of trout. Carbohydrate metabolism in these trout was studied and in addition, hemoglobin levels and red cell volumes were studied to serve as indicators of osmotic shifts of water.Increases in lactate were minimal and occurred during the first few hours of holding after the removal of [Formula: see text] to [Formula: see text] of the scales. There were slight changes in hemoglobin levels and red cell volumes which appeared to coincide with activity as reflected by lactate levels. There was a three- to fourfold increase in the blood glucose level of partially scaled fish. Liver glycogen levels dropped to [Formula: see text] of the air-dipped control value for fish that had been [Formula: see text] scaled and held in holding baskets for a period of 140 hr. Only one mortality occurred in all the experiments.The following conclusions were reached. Partial loss of scales or mucous does not result in the death of rainbow trout (Salmo gairdneri). Partial scaling or desliming of the fish was accompanied by a significant increase in blood glucose (p < 0.05).

LACTATE METABOLISM IN RAINBOW TROUT

1993 ◽  
Vol 180 (1) ◽  
pp. 175-193 ◽  
Author(s):  
C. L. Milligan ◽  
S. S. Girard
Keyword(s):  
Rainbow Trout ◽  
Blood Lactate ◽  
Muscle Glycogen ◽  
Lactate Clearance ◽  
Exhaustive Exercise ◽  
Muscle Lactate ◽  
Lactate Levels ◽  
Hepatic Portal

We have investigated the metabolic fate of blood lactate in resting rainbow trout and in fish recovering from a bout of exhaustive exercise. At rest and during recovery from exercise, the majority of blood lactate was oxidized, the proportion increasing with increasing oxygen consumption. It is estimated that, during recovery from exhaustive exercise, lactate released from the muscle has the potential to fuel a significant portion of oxidative metabolism. The bulk of the remaining blood lactate reappeared in the muscle lactate pool, probably via direct uptake by the muscle. There was a significant incorporation of blood lactate into the muscle glycogen pool, providing strong evidence for in situ glycogenesis as the mode for muscle glycogen replenishment. To investigate the role of the liver in blood lactate clearance, trout were functionally hepatectomized by ligation of the hepatic portal circulation. The exercise performance of hepatectomized fish was equal to that of sham- operated fish and controls, indicating that muscle relies primarily on endogenous fuel stores. Furthermore, blood lactate levels immediately after exercise were greater and muscle metabolic recovery was faster in hepatectomized fish than in sham-operated fish and controls. These observations suggest that glycogen resynthesis in trout muscle may be retarded because of a non- recoverable loss of substrate (i.e. lactate) from the muscle, because the lactate released is utilized by the liver. These results are discussed in view of what is known about these processes in other ectothermic vertebrates.

Muscle glycogen repletion during active postexercise recovery

1987 ◽  
Vol 253 (3) ◽  
pp. E305-E311 ◽  
Author(s):  
E. M. Peters Futre ◽  
T. D. Noakes ◽  
R. I. Raine ◽  
S. E. Terblanche
Keyword(s):  
Blood Lactate ◽  
Muscle Glycogen ◽  
High Intensity ◽  
Active Recovery ◽  
Muscle Lactate ◽  
Glycogen Resynthesis ◽  
Passive Recovery ◽  
Lactate Removal ◽  
Glycogen Repletion ◽  
Lactate Levels

High-intensity intermittent bicycle exercise was used to deplete muscle glycogen levels by 70% and elevate blood lactate levels to greater than 13.0 mmol/l. Thereafter subjects either cycled with one leg for 45 min followed by 45 min of passive recovery (partially active recovery) or rested for 90 min (passive recovery). During the first 45 min of partially active recovery 1) blood lactate (P less than 0.05) and pH levels (P less than 0.05) returned more rapidly to preexercise values than during passive recovery, 2) the rate of net glycogen resynthesis (0.28 mumol . g-1 . min-1) was the same in both legs, and 3) muscle lactate levels were significantly lower (P less than 0.05) in the passive than in the active leg. Thereafter the rate of net muscle glycogen resynthesis was unchanged (0.26 mumol . g-1 . min-1) and lactate removal could theoretically account for only 18% of the glycogen resynthesized. Overall, the rate of muscle glycogen resynthesis and muscle lactate removal was not different from that measured during passive recovery. After high-intensity exercise 1) glycogen repletion is not impeded by light exercise, and 2) blood glucose is an important substrate for glycogen resynthesis.

Changes in plasma insulin and carbohydrate metabolism of zinc-stressed rainbow trout, Salmo gairdneri

1982 ◽  
Vol 60 (9) ◽  
pp. 2079-2084 ◽  
Author(s):  
G. F. Wagner ◽  
B. A. McKeown
Keyword(s):  
Rainbow Trout ◽  
Pancreatic Beta Cells ◽  
Plasma Insulin ◽  
Zinc Sulphate ◽  
Liver Glycogen ◽  
Salmo Gairdneri ◽  
Control Group ◽  
Exposed Fish ◽  
Glucose Levels ◽  
Insulin Radioimmunoassay

An experiment was conducted to determine if the hyperglycemia that is observed in zinc-stressed fish is also accompanied by changes in the levels of plasma insulin and liver glycogen. Juvenile rainbow trout were exposed to three concentrations of zinc sulphate along with a control group over 31 days. Plasma glucose levels were monitored in each group over the course of the experiment. The group demonstrating the most acute and sustained hyperglycemia (0.352 ppm zinc) was then analyzed along with the controls for changes in plasma insulin (using a teleost insulin radioimmunoassay) and liver glycogen levels. Significant depressions in plasma insulin and liver glycogen levels were observed in the zinc-exposed fish when compared with the controls. These changes are discussed with respect to possible influences of epinephrine, which is elevated in stressed fish, and (or) a direct effect of zinc metal on the pancreatic beta cells.

Effects of Epinephrine on Carbohydrate Metabolism in Underfed and ad Libitum-Fed Rats

1957 ◽  
Vol 190 (2) ◽  
pp. 239-242
Author(s):  
B. N. Spirtos ◽  
R. G. Stuelke ◽  
N. S. Halmi
Keyword(s):  
Carbohydrate Metabolism ◽  
Blood Sugar ◽  
Blood Lactate ◽  
Liver Glycogen ◽  
Hepatic Glycogen ◽  
Commercial Diet ◽  
Ad Libitum ◽  
Sugar Levels ◽  
Fasted Rats

Rats fed 10 gm of a commercial diet for 4–5 weeks and fasted for 24 hours showed less rise in liver glycogen and blood sugar levels in response to the injection of epinephrine than did ad libitum-fed-fasted rats. Gastrocnemius glycogen levels were found to be higher in underfed-fasted animals and fell to the same extent as in ad libitum fed-fasted animals when epinephrine was given. Blood lactate concentrations, however, rose less markedly in the underfed-fasted group. This may have been at least partly responsible for the diminished rise in hepatic glycogen and blood sugar.

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