Immersion Vaccination of Sockeye Salmon (Oncorhynchus nerka) with Two Pathogenic Strains of Vibrio anguillarum

1979 ◽  
Vol 36 (2) ◽  
pp. 222-225 ◽  
Author(s):  
R. W. Gould ◽  
R. Antipa ◽  
D. F. Amend

Sockeye salmon (Oncorhynchus nerka) were immersion-vaccinated in suspensions containing 5 × 107, 5 × 106, 5 × 105, or 5 × 104 bacteria/mL of bivalent or monovalent, formalin-killed Vibrio anguillarum, Types I and II. The fish were split into two lots and held for 54 d. At that time one lot was challenged with living, virulent V. anguillarum, Type I, and one with living, virulent V. anguillarum, Type II. Immunization with bivalent bacterin effectively protected the fish from vibriosis, but monovalent vaccine was effective only against the homologous challenge. Immunization with the highest concentration of Type I monovalent bacterin resulted in 0% Type I and 58% Type II challenge mortality. Immunization with the highest concentration of Type II monovalent bacterin resulted in 41% Type I and 0% Type II challenge mortality. Immunization with the highest concentration of bivalent Type I/Type II bacterin resulted in 2% mortality in both challenges. Protective bacterins were effective at concentrations down to 5 × 105 bacteria/mL. Key words: immersion vaccination, bivalent vaccines, Vibrio anguillarum, vibriosis

1978 ◽  
Vol 35 (1) ◽  
pp. 148-154 ◽  
Author(s):  
Paul W. Reno ◽  
Marie Philippon-Fried ◽  
Bruce L. Nicholson ◽  
Stuart W. Sherburne

Erythrocytes of PEN-positive Atlantic herring (Clupea harengus harengus) were examined to determine their ultrastructure. Cytoplasmic inclusions were of two types when observed under the electron microscope. The first type (type I) appeared coarsely granular, electron dense, round, and up to 1.5 μm in diameter. Virions were closely associated with this type of inclusion. The second type of inclusion (type II) had approximately the same appearance as the surrounding cytoplasm, from which it was separated by a discrete membrane, and was variable in size. Virions were not intimately associated with type II inclusions. Virions occurred singly or in clusters within the cytoplasm or in association with type I inclusions and were hexagonal and 145 nm in diameter. Virions were composed of a rigid hexagonal capsid 8 nm wide, a lighter 16-nm region, and a core 100 nm in diameter. The virus of PEN is presumptively classified as an Iridovirus. Key words: ultrastructure, erythrocytes, virology


2002 ◽  
Vol 59 (4) ◽  
pp. 707-716 ◽  
Author(s):  
Marci L Koski ◽  
Brett M Johnson

In laboratory experiments, fingerling kokanee salmon (Oncorhynchus nerka, 3–8 g) were presented with varying densities of zooplankton prey (Daphnia spp.) ranging from 3 to 55 Daphnia·L–1, under three light intensities (30, 15, and 0.1 lx). Kokanee exhibited a type I functional response at 0.1 lx (Daphnia consumption·min–1 = 1.74 prey·L–1), a light level typical of moonlit epilimnetic conditions, but shifted to a type II functional response at higher light levels. Both 15 and 30 lx light levels occur during crepuscular periods when kokanee feeding is maximal in the wild, and consumption rates at these light levels were not significantly different (Daphnia consumption·min–1 = (163.6 prey·L–1)(42.2 prey·L–1)–1). The shift from the type I to type II functional response may be attributed to a foraging mode switch and the incorporation of search time instead of random encounters with prey. Using these models to simulate feeding rates in a Colorado reservoir, attenuation of light intensity and prey density between the epilimnion and hypolimnion resulted in a 100-fold increase in predicted feeding duration. Functional responses that incorporate environmental characteristics like light are important components of foraging models that seek to understand fish consumption, growth, and behavior.


1979 ◽  
Vol 36 (9) ◽  
pp. 1145-1149 ◽  
Author(s):  
Richard E. Thorne

Hydroacoustic techniques were used on Lake Washington from 1972 to 1975 to estimate the potential escapement of sockeye salmon (Oncorhynchus nerka). Target strength measurements were used to establish a threshold which would separate the larger adult sockeye salmon from smaller resident fish. The acoustic estimates of escapement were very similar to those obtained from visual observations at the Hiram M. Chittenden ship canal locks, observations on the Cedar River, and spawning ground surveys. Key words: hydroacoustic, acoustic, methodology, sockeye salmon, escapement


2020 ◽  
Vol 8 (4) ◽  
pp. 657
Author(s):  
Nur Shafika ◽  
Bustanul Arifin ◽  
Teguh Endaryanto

The main purposes of this research is to find out the characteristic of visitors, local workforces and business owners, the factors that affect the frequency of tourist visits, and to analyze the economic impact caused by the Youth Camp tourism activities. The location for the research is intentionally chosen Youth Camp in Taman Hutan Raya Wan Abdul Rachman. Contributing toward this research consists of 40 visitors, 10 people of local workforce, and 15 business owners. The data for the research were generated in December 2018 to February 2019 and analyzed using methods of descriptive qualitative and descriptive quantitative. The research findings showed that the Youth Camp visitors’ages range between 17-35 years old, originally came from Lampung Province, and on their final year in SMA or equivalent. Majority of them are students employed with income above IDR2,000,000.00, single and no liabilities responsibilities. The frequency of visitors who came to visit was mostly influenced by the distance, age and well known of the tourism place. The value impact obtained from the value of Keynesian Income Multiplier is 5.33, value of Ratio Income Multiplier Type I is 2.70, and value of Ratio Income Multiplier Type II is 3.83. Key words: economic impact, Keynesian multiplier, Youth Camp


2000 ◽  
Vol 80 (4) ◽  
pp. 749-752 ◽  
Author(s):  
P. J. Kononoff ◽  
A. F. Mustafa ◽  
D. A. Christensen ◽  
J. J. McKinnon

A study was conducted to determine the effects of two theoretical lengths of particle (TLP) of barley silage on physically effective neutral detergent fiber (peNDF) content and on yield and composition of milk in dairy cows. Type I peNDF uses the neutral detergent fiber (NDF) of forage materials retained on a 1.18-mm screen while Type II peNDF is based on the NDF content of three different particle size fractions. Cows received diets consisting of 55% concentrate and 45% barley silage of either a 9.5-mm or 4.8-mm TLP. Results showed no difference in Type I peNDF between the two barley silage TLP. However, barley silage with a 9.5 mm TLP contained more (P < 0.05) Type II peNDF than barley silage with a 4.8 mm TLP. Differences in particle size or Type II peNDF content of barley silage had no effect on yield or composition of milk in dairy cows. Key words: Particle size, physically effective fiber, dairy cows


1977 ◽  
Vol 34 (5) ◽  
pp. 706-709 ◽  
Author(s):  
Norbert P. Boyce ◽  
S. Behrens Yamada

Most research to date on the tolerance of natural populations to physical and chemical stressors appears to have overlooked that parasites may themselves be stressors, and that they may be capable of modifying the resistance of the host to other applied stressors. Sockeye salmon (Oncorhynchus nerka) smolts trapped at the outlet of Babine Lake, central British Columbia, were subjected to 1 mg/ℓ dissolved zinc under laboratory conditions. Smolts infected with the intestinal cestode Eubothrium salvelini proved to be significantly more susceptible to zinc than were noninfected smolts. We recommend that future investigations of environmental effects, especially on wild populations, include consideration of the state of parasitism of the test organisms. Key words: Eubothrium salvelini, cestode, sockeye salmon, Babine Lake, zinc, toxicity


1980 ◽  
Vol 37 (4) ◽  
pp. 561-566 ◽  
Author(s):  
Randall M. Peterman

Basic components of predation processes are briefly reviewed and data on these components are presented for several native Indian food fisheries. Depensatory mortality emerges as a common effect of these fisheries, which supports findings of earlier workers on Skeena River sockeye salmon (Oncorhynchus nerka). Native food fisheries therefore operate like natural "Type II" predators, and descriptive equations are provided for use by salmon managers and modelers.Key words: fishermen as predators, native, Indian, food, fisheries, salmon, depensation


Author(s):  
Ronald S. Weinstein ◽  
N. Scott McNutt

The Type I simple cold block device was described by Bullivant and Ames in 1966 and represented the product of the first successful effort to simplify the equipment required to do sophisticated freeze-cleave techniques. Bullivant, Weinstein and Someda described the Type II device which is a modification of the Type I device and was developed as a collaborative effort at the Massachusetts General Hospital and the University of Auckland, New Zealand. The modifications reduced specimen contamination and provided controlled specimen warming for heat-etching of fracture faces. We have now tested the Mass. General Hospital version of the Type II device (called the “Type II-MGH device”) on a wide variety of biological specimens and have established temperature and pressure curves for routine heat-etching with the device.


Author(s):  
G. D. Gagne ◽  
M. F. Miller ◽  
D. A. Peterson

Experimental infection of chimpanzees with non-A, non-B hepatitis (NANB) or with delta agent hepatitis results in the appearance of characteristic cytoplasmic alterations in the hepatocytes. These alterations include spongelike inclusions (Type I), attached convoluted membranes (Type II), tubular structures (Type III), and microtubular aggregates (Type IV) (Fig. 1). Type I, II and III structures are, by association, believed to be derived from endoplasmic reticulum and may be morphogenetically related. Type IV structures are generally observed free in the cytoplasm but sometimes in the vicinity of type III structures. It is not known whether these structures are somehow involved in the replication and/or assembly of the putative NANB virus or whether they are simply nonspecific responses to cellular injury. When treated with uranyl acetate, type I, II and III structures stain intensely as if they might contain nucleic acids. If these structures do correspond to intermediates in the replication of a virus, one might expect them to contain DNA or RNA and the present study was undertaken to explore this possibility.


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