Molecular diversity and relationships among Elymus trachycaulus, E. subsecundus, E. virescens, E. violaceus, and E. hyperarcticus (Poaceae: Triticeae) as determined by amplified fragment length polymorphism

Genome ◽  
2006 ◽  
Vol 49 (9) ◽  
pp. 1160-1169 ◽  
Author(s):  
Genlou Sun ◽  
Jennifer Shee ◽  
Bjorn Salomon
Keyword(s):  
Molecular Diversity ◽  
Genetic Relationships ◽  
Morphological Characters ◽  
Mean Value ◽  
Aflp Analysis ◽  
Neighbor Joining ◽  
Elymus Trachycaulus ◽  
The Mean ◽  
High Degree ◽  
Mean Similarity

Morphological similarity among E. trachycaulus, E. virescens, E. violaceus, and E. hyperarcticus has often been noted. Taxonomists have tried to discriminate among these taxa using morphological characters and a number of different relationships among them have been suggested. However, the genetic relationships among these taxa are still unknown. AFLP analysis was used to characterize the molecular diversity of these taxa and to examine genetic relationships among them. A high degree of genetic identity was apparent among 7 accessions of E. virescens. The similarity values ranged from 0.90 to 0.99 with an average of 0.94. The mean similarity values among 3 E. hyperarcticus and among 5 E. violaceus accessions were 0.84 (0.81–0.87) and 0.77 (0.66–0.90), respectively. The similarity values among 17 E. trachycaulus accessions ranged from 0.49 to 0.92 with an average of 0.75. The 5 accessions of E. subsecundus displayed high variation, with similarity values between 0.52 and 0.68 and a mean value of 0.59. Both maximum-parsimony (MP) and neighbor-joining (NJ) analyses showed that all 7 accessions of E. virescens formed a clade, indicating a monophyletic origin. On the other hand, Elymus trachycaulus, E. subsecundus, and E. violaceus were each paraphyletic and separated into different genetically distinct groups. Among these 5 taxa, E. virescens was genetically similar to E. trachycaulus, and E. violaceus was genetically similar to E. hyperarcticus.

scholarly journals Assessment of genetic diversity in Indian Barnyard millet (Echinochloa spp. complex) using morphological and molecular markers

2016 ◽  
Vol 8 (3) ◽  
pp. 1643-1648 ◽  
Author(s):  
M. P. Moharil ◽  
Dipti Gawai ◽  
N. Dikshit ◽  
M.S. Dudhare ◽  
P. V. Jadhav
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Genetic Improvement ◽  
Molecular Diversity ◽  
Genetic Relationships ◽  
Barnyard Millet ◽  
Clear Cut ◽  
Cultivated Species ◽  
High Degree

In the present study, morphological and molecular markers (RAPD primers) were used to analyze the genetic diversity and genetic relationships among 21 accessions of Echinochloa spp. complex comprising the wild and cultivated species collected from Melghat and adjoining regions of Vidarbha, Maharashtra. The availability of diverse genetic resources is a prerequisite for genetic improvement of any crop including barnyard millet. A high degree of molecular diversity among the landraces was detected. Among the 21 genotypes, two major groups (A and B) were formed, at 67.28 % similarity, which clearly encompasses 15 accessions of E. frumentacea and 6 accessions of E. colona. Higher similarity was observed in accessions of E. frumentacea. The accessions IC 597322 and IC 597323 also IC 597302 and IC 597304 showed more than 94% similarity among themselves. The classification of genetic diversity has enabled clear-cut grouping of barnyard millet accessions into two morphological races (E. frumentacea and E. colona).

scholarly journals Genetic Characterization and Diversity of Rathayibacter toxicus

2006 ◽  
Vol 96 (11) ◽  
pp. 1270-1277 ◽  
Author(s):  
I. V. Agarkova ◽  
A. K. Vidaver ◽  
E. N. Postnikova ◽  
I. T. Riley ◽  
N. W. Schaad
Keyword(s):  
Fragment Length Polymorphism ◽  
Geographic Origin ◽  
Aflp Analysis ◽  
Pfge Analysis ◽  
Positive Bacterium ◽  
Banding Patterns ◽  
History Of ◽  
Genetic Clusters ◽  
The Mean ◽  
Mean Similarity

Rathayibacter toxicus is a nematode-vectored gram-positive bacterium responsible for a gumming disease of grasses and production of a highly potent animal and human toxin that is often fatal to livestock and has a history of occurring in unexpected circumstances. DNA of 22 strains of R. toxicus from Australia were characterized using amplified fragment length polymorphism (AFLP) and pulsed-field gel electrophoresis (PFGE). AFLP analysis grouped the 22 strains into three genetic clusters that correspond to their geographic origin. The mean similarity between the three clusters was 85 to 86%. PFGE analysis generated three different banding patterns that enabled typing the strains into three genotypic groups corresponding to the same AFLP clusters. The similarity coefficient was 63 to 81% for XbaI and 79 to 84% for SpeI. AFLP and PFGE analyses exhibited an analogous level of discriminatory power and produced congruent results. PFGE analysis indicated that the R. toxicus genome was represented by a single linear chromosome, estimated to be 2.214 to 2.301 Mb. No plasmids were detected.

scholarly journals A CHANGE OF THE LENGTH OF THE ANTERO-POSTERIOR AXIS OF THE EYE IN THE CHILDREN PRESENTING WITH HYPEROPIA FOLLOWING FEMTOSECOND LASER-ASSISTED IN SITU KERATOMILEUSIS: 3 YEAR FOLLOW-UP

2018 ◽  
Vol 13 (1) ◽  
pp. 31-37
Author(s):  
I. L Kulikova ◽  
N. P Pashtaev ◽  
Anastasiya Vladimirovna Gagloeva ◽  
O. V Shlenskaya ◽  
N. V Chapurin
Keyword(s):  
Femtosecond Laser ◽  
Mean Value ◽  
Minimal Growth ◽  
The Mean ◽  
Group 2 ◽  
Contralateral Eye ◽  
High Degree ◽  
Positive Results

Aim. The objective of the present study was the analysis of the changes in the length of the antero-posterior axis of the eye (AL) in the children and adolescents presenting with hyperopia and anisometropia in 3 years after femtolaser-assisted in situ keratomileusis (FS-LASIK). Material and methods. The study included 33 patients at the age from 5 to 16 years divided into two groups. Group 1 was comprised of the patients having initially the mean spherical refractive equivalent (SE) +3.69 D. Those of group 2 had initial SE +5.88 D. In all the patients, FS-LASIK was carried out at the amblyopic eye in the absence of the positive results of the conventional conservative treatment. Results. During three years after FS-LASIK, AL of the treated patients in the first group increased by 0.41 mm and reached the mean value of 22.41 mm while the spherical refractive equivalent became equal to 0.25 D. During the same period, AL in the contralateral eye increased by 0.92 mm and became equal to 23.47 mm on the average with SE equaling +0.81 D. AL of the patients in the second group increased by 0.29 mm and reached the mean value of 21.59 mm while the spherical refractive equivalent became equal to 0.25 D. During the same period, AL in the contralateral eye increased by 0.97 mm and became equal to 23.32 mm on the average with SE equaling +0.62 D. Conclusion. During three years after hyperopic S-LASIK, the minimal growth of the antero-posterior axis of the eye was documented in the children presenting with hyperopia (+5 D) whereas its length increased by more than 0.29 mm. In the children with hyperopia of less than +5 D, it increased by 0.41 mm. It is concluded that prognosis of the results of femtosecond laser-assisted in situ keratomileusis leading to the alteration of the refraction in the amblyopic eye with the initially moderate or high-degree hyperopia should be made taking into consideration that AL continues to change in the cource of further growth of the child.

scholarly journals PROPORSI MIKROSPORA UNINUKLEAT PADA EMPAT KLON TEBU (Saccharum spp.)

2007 ◽  
Vol 12 (2) ◽  
pp. 145-152
Author(s):  
Suaib Suaib ◽  
Woerjono Mangoendidjojo ◽  
PDN Mirzawan ◽  
Ari Indrianto
Keyword(s):  
Tissue Culture ◽  
Standard Deviation ◽  
Flag Leaf ◽  
Pollen Grains ◽  
Morphological Characters ◽  
Mean Value ◽  
Microspore Development ◽  
Saccharum Spp ◽  
The Mean ◽  
Uninucleate Microspore

An experiment to study the two different morphological characters of four clones of sugarcane (Saccharum spp.) panicles containing more than 50 percent of uninucleate microspore development was conducted in Tissue Culture Laboratory at Biology Faculty, Gadjah Mada University, Yogyakarta, since March until May 2006. Morphological characters of both kinds of panicles i.e. unsheated- and sheated flowers from sheat flag leaf were observed. Mean, percentage, and standard deviation from the mean value of the six different stages of microspore development e.g. tetrad, early- and late-uninucleate, early- and late-binucleate, and multinucleate or pollen grains were statistically used in this calculation. All data percentages were analyzed by variance analysis through General Linier Model Procedure, and comparisons between means of the uninucleate microspore development based on the two different morphological characters of four clones was calculated by Least Significance Difference method. Comparisons between the two different panicles characteristics in accordance with the proportion of the uninucleate microspore development, however, were analyzed by T-student procedure. All calculations were done by using SAS program of computer statistics package. Result of the research showed that: (1) the unsheated panicles were contained less than 50 percent of uninucleate (early- and late-uninucleate) microspore development; (2) the sheated panicles tend to be in high proportion of early- and late-uninucleate microspore development, and multinucleate or pollen grains, and (3) the more away of spikelets or anthers positioned in the panicle or sub-panicle, the more number or percentage of uninucleate microspores development were tend to be gradually decreased.

scholarly journals Genetic diversity and relationships of Chinese donkeys using microsatellite markers

2019 ◽  
Vol 62 (1) ◽  
pp. 181-187 ◽  
Author(s):  
Lulan Zeng ◽  
Ruihua Dang ◽  
Hong Dong ◽  
Fangyu Li ◽  
Hong Chen ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Genetic Relationships ◽  
Mean Value ◽  
Principal Coordinates Analysis ◽  
Expected Heterozygosity ◽  
Principal Coordinates ◽  
Baseline Information ◽  
Grouping Pattern ◽  
The Mean

Abstract. Donkeys are one important livestock in China because of their nourishment and medical values. To investigate the genetic diversity and phylogenetic relationships of Chinese donkey breeds, a panel of 25 fluorescently labeled microsatellite markers was applied to genotype 504 animals from 12 Chinese donkey breeds. A total of 226 alleles were detected, and the expected heterozygosity ranged from 0.6315 (Guanzhong) to 0.6999 (Jiami). The mean value of the polymorphism information content, observed number of alleles, and expected number of alleles for all the tested Chinese donkeys were 0.6600, 6.890, and 3.700, respectively, suggesting that Chinese indigenous donkeys have relatively abundant genetic diversity. Although there were abundant genetic variations found, the genetic differentiation between the Chinese donkey breeds was relatively low, which displayed only 5.99 % of the total genetic variance among different breeds. The principal coordinates analysis clearly splits 12 donkey breeds into two major groups. The first group included Xiji, Xinjiang, Liangzhou, Kulun, and Guanzhong donkey breeds. In the other group, Gunsha, Dezhou, Biyang, Taihang, Jiami, Qingyang, and Qinghai donkeys were clustered together. This grouping pattern was further supported by structure analysis and neighbor-joining tree analysis. Furthermore, genetic relationships between different donkey breeds identified in this study were corresponded to their geographic distribution and breeding history. Our results provide comprehensive and precise baseline information for further research on preservation and utilization of Chinese domestic donkeys.

scholarly journals A redetermination of the velocities of α particles from radium C, thorium C and C'

1928 ◽  
Vol 118 (780) ◽  
pp. 549-557 ◽  
Keyword(s):  
Electric Fields ◽  
Experimental Error ◽  
Atomic Weight ◽  
Mean Value ◽  
Experimental Result ◽  
Slow Moving ◽  
The Mean ◽  
High Degree ◽  
Active Substances ◽  
Α Particles

1. The first accurate determinations of the velocity with which an α particle is expelled from a radio-active substance and of the value of E/M, the ratio of the charge to the mass, were made by Rutherford and Robinson by measuring the deflections in magnetic and electric fields. An α particle moving with velocity V perpendicular to the direction of a magnetic field H describes a circle of radius ρ where H ρ = MV/E. Rutherford and Robinson found for α particles expelled from radium C Hρ = 3·983 X 10 5 E. M. U. and V = 1·922 X 10 9 cm. per second. The mean value of E/M for α particles from radium emanation, radium A and radium C was 4820 E. M. U., which agreed to within the limits of experimental error with the value 4826 deduced from electrochemical data taking the atomic weight of helium as 3·998 and the value of the faraday as 9647. This value of the velocity of α particles from radium C has served as a standard from which the velocities of α particles from other radio-active substances have been calculated from the Geiger relation V 3 = k R. The present paper gives an account of a redetermination of the quantity Hρ for α particles from radium C by a method which is essentially similar to those used in previous determinations of this kind. From the value of Hρ the velocity has been calculated using the theoretical value of E/M which can be found to a high degree of accuracy from more recent determinations of the atomic weight of helium and the value of the faraday, taking into account the relativity correction for the increase in mass of the α particle. For the atomic weight of helium we may take 4·000, the value deduced by Van Laar from a consideration of the density determinations of Watson, Heuse, Taylor, and in particular of Guye’s discussion of Taylor’s results. Taking the value of the faraday given by recent determinations 9649·4 E. M. U. the value of E/M for a slow moving α particle is 4824·7 and on applying the correction for the relativity change of mass this becomes 4814·8 for the α particle from radium C, neglecting the mass of the lost electrons. It may be noted that Rutherford and Robinson’s experimental result, 4820 agrees as well with this latter value as with the value 4826 which they calculated.

scholarly journals Transferrin variation and genetic structure of reindeer populations in Scandinavia

Rangifer ◽  
1987 ◽  
Vol 7 (1) ◽  
pp. 12 ◽  
Author(s):  
Knut H. Røed ◽  
T. Mossing ◽  
M. Nieminen ◽  
A. Rydberg
Keyword(s):  
Genetic Distance ◽  
Polyacrylamide Gel Electrophoresis ◽  
Mean Value ◽  
Allele Frequency Distribution ◽  
Wild Populations ◽  
Allele Distribution ◽  
The Mean ◽  
Different Origins ◽  
High Degree ◽  
Wild Reindeer

<p>Polyacrylamide gel electrophoresis was used to analyse transferrin variation in herds of semi-domestic reindeer from Scandinavia. The results are compared with previously reported values for other populations of both semi-domestic and wild reindeer using the same techniques as in the present study. In all populations the number of alleles was high, ranging from seven to eleven, and the heterozygosity was correspondingly high, with a mean of 0.749. This high genetic variation in all populations suggests that inbreeding is not widespread among Scandinavian reindeer. The pattern of allele frequency distribution indicates a high degree of genetic heterogeneity in the transferrin locus, both between the different semi-domestic herds and between the different wild populations. The mean value of genetic distance was 0.069 between semi-domestic herds and 0.091 between wild populations. Between semi-domestic and wild populations the genetic distance was particularly high, with a mean of 0.188. This high value was mainly due to a different pattern in the distribution of the two most common transferrin alleles: Tfu was most common among semi-domestic herds, while TfEI was most common among wild populations. These differences in transferrin allele distribution are discussed in relation to possible different origins of semi-domestic and wild reindeer in Scandinavia, or alternatively, to different selection forces acting on transferrin genotypes in semi-domestic and wild populations.</p><p>Transferrin-variasjon og genetisk struktur hos rein i Skandinavia.</p><p>Abstact in Norwegian / Sammendrag: Transferrin-variasjon i tamreinflokker ble analysert ved hjelp av polyacrylamid gel elektroforese. Resultatene er sammenlignet med verdier som tidligere er beskrevet for b&aring;de tamrein og villrein hvor det ble benyttet samme metode som i denne unders&oslash;kelsen. I alle populasjonene ble det registrert et h&oslash;yt antall alleler (7-11) og heterozygositeten var tilsvarende h&oslash;y med en middelverdi p&aring; 0.749. Denne h&oslash;ye graden av genetisk variasjon i alle unders&oslash;kte populasjoner indikerer at det ikke foreg&aring;r mye innavl blant rein i Skandinavia. Utbredelsen av de enkelte allelene viste h&oslash;y grad av genetisk oppdeling i transferrin-locuset mellom flokker av b&aring;de tamrein og villrein. Middelverdien for genetisk avstand var 0.069 mellom tamreinflokker og 0.091 mellom villreinflokker. S&aelig;rlig stor genetisk avstand (middelverdi 0.188) ble funnet mellom tamrein og villrein. Denne store forskjellen skyldes i stor grad forskjellig m&oslash;nster i utbredelsen av de to vanligste allelene: Tf' var mest vanlig blant tamrein og Tf1' var mest vanlig blant villrein. Denne forskjellen er diskutert i relasjon til forskjellig opprinnelse av tamrein og villrein og alternativt, i relasjon til forskjellig seleksjonskrefter som virker p&aring; transferrin genotyper i tamrein og villrein.</p>

Long-period modulated superstructures in Pd-12.5 at.%Ce and Pd-15 at.%Ce alloys

1990 ◽  
Vol 48 (4) ◽  
pp. 164-165
Author(s):  
Noriyuki Kuwano ◽  
Masaru Itakura ◽  
Kensuke Oki
Keyword(s):  
Electron Microscopy ◽  
Mean Value ◽  
Heavy Elements ◽  
Arc Furnace ◽  
X Ray ◽  
Long Period ◽  
Ultra High Purity ◽  
Heat Treated ◽  
Atomic Scattering ◽  
The Mean

Pd-Ce alloys exhibit various anomalies in physical properties due to mixed valences of Ce, and the anomalies are thought to be strongly related with the crystal structures. Since Pd and Ce are both heavy elements, relative magnitudes of (fcc-fpd) are so small compared with <f> that superlattice reflections, even if any, sometimes cannot be detected in conventional x-ray powder patterns, where fee and fpd are atomic scattering factors of Ce and Pd, and <f> the mean value in the crystal. However, superlattices in Pd-Ce alloys can be analyzed by electron microscopy, thanks to the high detectability of electron diffraction. In this work, we investigated modulated superstructures in alloys with 12.5 and 15.0 at.%Ce.Ingots of Pd-Ce alloys were prepared in an arc furnace under atmosphere of ultra high purity argon. The disc specimens cut out from the ingots were heat-treated in vacuum and electrothinned to electron transparency by a jet method.

Partition Coefficient Ratios and Tumour Perfusion Studied with 85mKr and 133Xe

1987 ◽  
Vol 26 (06) ◽  
pp. 253-257
Author(s):  
M. Mäntylä ◽  
J. Perkkiö ◽  
J. Heikkonen
Keyword(s):  
Partition Coefficient ◽  
Partition Coefficients ◽  
Regional Blood Flow ◽  
Blood Perfusion ◽  
Compartmental Analysis ◽  
Malignant Tumour ◽  
Mean Value ◽  
Perfusion Rate ◽  
Biological Variables ◽  
The Mean

The relative partition coefficients of krypton and xenon, and the regional blood flow in 27 superficial malignant tumour nodules in 22 patients with diagnosed tumours were measured using the 85mKr- and 133Xe-clearance method. In order to minimize the effect of biological variables on the measurements the radionuclides were injected simultaneously into the tumour. The distribution of the radiotracers was assumed to be in equilibrium at the beginning of the experiment. The blood perfusion was calculated by fitting a two-exponential function to the measuring points. The mean value of the perfusion rate calculated from the xenon results was 13 ± 10 ml/(100 g-min) [range 3 to 38 ml/(100 g-min)] and from the krypton results 19 ± 11 ml/(100 g-min) [range 5 to 45 ml/(100 g-min)]. These values were obtained, if the partition coefficients are equal to one. The equations obtained by using compartmental analysis were used for the calculation of the relative partition coefficient of krypton and xenon. The partition coefficient of krypton was found to be slightly smaller than that of xenon, which may be due to its smaller molecular weight.

Detection of Soluble Fibrin Monomer Complexes in Blood by Means of Protamine Sulphate Test

1968 ◽  
Vol 20 (01/02) ◽  
pp. 044-049 ◽  
Author(s):  
B Lipiński ◽  
K Worowski
Keyword(s):  
Human Subjects ◽  
Coronary Thrombosis ◽  
Mean Value ◽  
Healthy Human ◽  
Protamine Sulphate ◽  
Soluble Fibrin ◽  
Fibrin Monomer ◽  
Dog Plasma ◽  
The Mean ◽  
Precipitable Protein

SummaryIn the present paper described is a simple test for detecting soluble fibrin monomer complexes (SFMC) in blood. The test consists in mixing 1% protamine sulphate with diluted oxalated plasma or serum and reading the optical density at 6190 Å. In experiments with dog plasma, enriched with soluble fibrin complexes, it was shown that OD read in PS test is proportional to the amount of fibrin recovered from the precipitate. It was found that SFMC level in plasma increases in rabbits infused intravenously with thrombin and decreases after injection of plasmin with streptokinase. In both cases PS precipitable protein in serum is elevated indicating enhanced fibrinolysis. In healthy human subjects the mean value of OD readings in plasma and sera were found to be 0.30 and 0.11, while in patients with coronary thrombosis they are 0.64 and 0.05 respectively. The origin of SFMC in circulation under physiological and pathological conditions is discussed.

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