Organisation de la variabilité enzymatique des populations marocaines de luzerne (Medicago sativa L.): structures géniques et génotypiques

Genome ◽  
1989 ◽  
Vol 32 (1) ◽  
pp. 120-128 ◽  
Author(s):  
A. Birouk ◽  
Y. Dattée
Keyword(s):  
Medicago Sativa ◽  
Significant Role ◽  
Allelic Diversity ◽  
Low Frequency ◽  
The Other ◽  
Good Discrimination ◽  
Mountainous Regions ◽  
Hardy Weinberg Equilibrium ◽  
Medicago Sativa L ◽  
Fixation Indices

Five enzymatic loci have been analyzed electrophoretically to study the genic and genotypic structures of 20 populations of alfalfa (Medicago sativa L.) from Morocco. Five different geographical origins were considered: Ziz, Drâa and Dadès (three oases in the Sahara), Demnate, and oases from mountainous regions. For comparison, three cultivars (African, Moapa, Europe) and four populations (one from Sudan, two from Spain, and one from Provence) of foreign origin were included in the study. The genic structures of the above 27 populations or cultivars allowed a good discrimination among populations and origins. The alleles with low frequency played a significant role in the discrimination and the discrimination was better when established on the basis of allelic diversity than on genotypic structures. The Flemish cultivar Europe differed from all other mediterranean material (African, Moapa, Provence, Morocco populations) but closely resembled two Spanish ecotypes from Mielga. The cultivars African and Moapa proved to be different from the Morocco populations. The latter could be divided into two different genic pools: one consisted of the Demnate populations, the other grouped populations of the Sahara and mountainous oases with no clear distinction between geographical origins. Genotypic structures based on the analyses of 30 plants from each population or cultivar did not allow to discriminate among populations. To study fixation indices and verify the panmictic equilibrium hypothesis, the number of specimen per population or cultivar had to be increased for a few populations. All the populations or cultivars analyzed showed a lack of heterozygous plants and the hypothesis of a Hardy–Weinberg equilibrium could not be accepted. Frequencies of monogenic individuals and digenic duplexes were high, but tetragenic individuals were infrequent.Key words: alfalfa, isozymes, genic structures, genotypic structures, infrequent alleles.[Journal translation]

Phosphorus seed coatings for pasture species. II. Comparison of effectiveness of phosphorus applied as seed coatings, drilled or broadcast, in promoting early growth of phalaris (Phalaris aquatica L.) and lucerne (Medicago sativa L.)

1988 ◽  
Vol 39 (3) ◽  
pp. 447 ◽  
Author(s):  
JM Scott ◽  
GJ Blair
Keyword(s):  
Medicago Sativa ◽  
Plant Height ◽  
Early Growth ◽  
The Other ◽  
Fertilizer Treatment ◽  
Seed Coating ◽  
Phalaris Aquatica ◽  
Medicago Sativa L ◽  
Application Methods ◽  
P Sources

The relative efficiency of phosphorus (P) seed coatings and drilled and broadcast applications of P was investigated in a glasshouse experiment conducted with phalaris (Phalaris aquatica L.) and lucerne (Medicago sativa L.) in P-deficient soil. Two P sources (monocalcium phosphate [MCP] and dicalcium phosphate [DCP]) were used at three rates in seed coatings (0, 5 and 10 kg P ha-1) or at six rates each of drilled or broadcast applications (0, 2.5, 5, 10, 20 and 40 kg P ha-1). MCP coatings severely reduced lucerne emergence and hence yield, but had little effect on phalaris emergence. No other fertilizer treatment affected emergence. The height of phalaris plants at 27 days after sowing (d.a.s.) was increased as much by seed coatings containing MCP at 5 kg P ha-1 as by drill or broadcast applications of MCP at 20 kg P ha-1. Plant height of the MCP seed coating treatments was also less variable than the other application methods, suggesting that the coatings provided more uniform access to P. Seed coatings containing DCP at 10 kg P ha-1 increased phalaris plant height more than did drilled or broadcast applications at 40 kg P ha-1, but there was no effect of any DCP treatment on lucerne growth at 27 d.a.s. The relatively large differences observed in plant height between different application methods at 27 d.a.s. diminished over time until, at 57 d.a.s., only small differences were evident. The dry matter yield and P content of both species were increased by increasing rates of MCP, but DCP had little effect at any rate. The reduced differences between the seed coating treatments and the other application methods at harvest may have been due to the limited soil volume of the pots used; further work is necessary in larger soil volumes to determine the significance of the early growth advantage conferred on phalaris by the phosphorus seed coatings, and to find ways of protecting lucerne from soluble P sources during germination.

Genetics, cytology, and crossing behavior of an alfalfa (Medicago sativa) mutant resulting in failure of the postmeiotic cytokinesis

1983 ◽  
Vol 25 (4) ◽  
pp. 390-397 ◽  
Author(s):  
T. J. McCoy ◽  
L. Y. Smith
Keyword(s):  
Medicago Sativa ◽  
Low Frequency ◽  
Recessive Gene ◽  
Microspore Mother Cell ◽  
Developmental Sequence ◽  
Single Recessive Gene ◽  
Single Nucleus ◽  
Medicago Sativa L ◽  
Jumbo Pollen ◽  
Is Failure

During pollen investigations on diploid alfalfa (Medicago sativa L.) several plants were identified which produced "jumbo" pollen. The cause of the jumbo pollen is failure of the postmeiotic cytokinesis. These plants produce a single, four-nucleate microspore from one microspore mother cell (MMC) rather than the normal four, single-nucleate microspores from one MMC. Subsequent gametophyte development is characterized by fusion of the four nuclei into a single nucleus in most cases (range of 80 to 100%), followed by a developmental sequence comparable to normal alfalfa. Mature 4n male gametophytes are thus formed from 2n sporophytes. Genetic control of the postmeiotic cytokinesis failure is by a single recessive gene, designated jp. Although a low frequency of jumbo pollen does germinate (range of 3.1 to 37.8%), crossing studies demonstrate jumbo pollen is incapable of effecting fertilization. The use of the jp mutant in breeding studies, and interspecific hybridization research, is discussed.

Evaluation of Rhizobium inoculant formulations for alfalfa yield and N fixation

2007 ◽  
Vol 87 (2) ◽  
pp. 267-272 ◽  
Author(s):  
J. Diane Knight
Keyword(s):  
High Risk ◽  
Medicago Sativa ◽  
Sinorhizobium Meliloti ◽  
The Other ◽  
N Fixation ◽  
Biological N Fixation ◽  
Small Seed ◽  
Alfalfa Seed ◽  
Medicago Sativa L

Because of its small seed size, alfalfa (Medicago sativa L.) typically is seeded at a shallow depth, putting Rhizobium inoculated onto the seed coat at a high risk of desiccation. Granular inoculants may provide a superior delivery formulation for Rhizobium because the inoculant can be placed deeper in the soil than the seed, where it is protected from desiccation. Sinorhizobium meliloti cv. Beaver delivered as (1) pre-inoculated alfalfa seed from the inoculant manufacturer, (2) commercial peat-based, self-sticking inoculant applied on-site, and (3) granular inoculant placed with the seed or (4) banded below and to the side of the seed was evaluated against uninoculated alfalfa controls at three field sites in Saskatchewan. Overall, alfalfa inoculated with the granular formulation placed below and to the side of the alfalfa seed was among the highest biomass producer in the establishment year, although it did not exhibit superior nodulation or biological N fixation compared with the other treatments. Any advantage conferred by the granular inoculant in the establishment year did not persist through the first and second production years. Under the conditions of this study, the delivery formulation had no long-term affect on alfalfa productivity, nodulation and biological N fixation. Furthermore, none of the inoculant treatments were consistently superior to the uninoculated controls. Key words: Alfalfa, granular inoculant, peat inoculant, pre-inoculated seed, Medicago sativa L., Sinorhizobium meliloti

EFFECT OF GENERATION AND LOCATION OF SEED PRODUCTION ON GROWTH HABIT AND ANTHRACNOSE RESISTANCE IN ARC ALFALFA

1986 ◽  
Vol 66 (3) ◽  
pp. 585-589
Author(s):  
J. H. ELGIN Jr. ◽  
C. M. RINCKER
Keyword(s):  
Medicago Sativa ◽  
Seed Production ◽  
Growth Habit ◽  
The Other ◽  
Anthracnose Resistance ◽  
Field Growth ◽  
Medicago Sativa L ◽  
Growth Habits ◽  
Fall Growth

Thirty-one seedlots of Arc alfalfa (Medicago sativa L.), produced between 1971 and 1974 at Prosser, Wash.; Stanfield, Oreg.; and Shafter and Tehachapi, Calif., were evaluated for shifts in field growth habits (regrowth after cutting, winter injury, and date of flowering) and greenhouse anthracnose resistance. Seedlots represented one (Syn 2), two (Syn 3), and three (Syn 4) generations of increase beyond the original Syn 1 lot, grown under four diverse environments. Results indicated a tendency for shift in regrowth rate among the Syn 3 and Syn 4 generation seedlots produced at Shafter, Calif. No shift in regrowth rate was observed for the other locations and no significant trends away from the original lot were observed for winter injury, date of flowering or anthracnose resistance.Key words: Medicago sativa L., seed production, genetic shifts, anthracnose resistance, fall growth, regrowth

THE STAGES AT WHICH FROST INJURY OCCURS IN ALFALFA

1966 ◽  
Vol 44 (11) ◽  
pp. 1471-1483 ◽  
Author(s):  
C. G. Greenham
Keyword(s):  
Medicago Sativa ◽  
Cell Membranes ◽  
Low Frequency ◽  
Maximum Resistance ◽  
Frost Injury ◽  
Medicago Sativa L ◽  
Frozen State ◽  
Minimum Resistance

This investigation deals with the injury caused to four varieties of alfalfa (Medicago sativa L.), under freezing–thawing conditions comparable with those of Australian tableland regions.Evidence is presented to show that the intactness of cell membranes influences the magnitude of the low-frequency resistance attained in frozen tissues.Measurements of the maximum resistance attained during freezing, the proportionate drop in resistance after freezing, and the minimum resistance attained after freezing, are considered in relation to two subsequently determined criteria of injury. The results are interpreted as showing that injury occurs in tissue during freezing, in the frozen state, and during thawing.Brief comments follow concerning causes of injury at the various stages, factors responsible for hardiness, and the procedure necessary for selecting the most hardy genotype.

HEXAPLOID ALFALFA, MEDICAGO SATIVA L.: ORIGIN, FERTILITY AND CYTOLOGY

1969 ◽  
Vol 11 (2) ◽  
pp. 359-366 ◽  
Author(s):  
E. T. Bingham ◽  
A. Binek
Keyword(s):  
Medicago Sativa ◽  
Low Frequency ◽  
Field Testing ◽  
Pollen Stainability ◽  
Ovule Number ◽  
Medicago Sativa L ◽  
Seed Increase ◽  
Genetic Bases

Two spontaneously occurring hexaploids, 6-1 and 6-2, 2n = 6x = 48, were discovered among tetraploid plants of the variety 'Saranac'. They were selfed, crossed with each other and with diploid (2x), triploid (3x), and tetraploid (4x) alfalfa. Self and cross progeny of 6-1 and 6-2 were all hexaploid; 2x-6x crosses produced a plant with 32 chromosomes; 3x-6x crosses produced hexaploids probably due to unreduced triploid gametes; and 4x-6x crosses produced plants with 32, 34, 35, 36, 37, 38, 40, and 56 chromosomes. Thus many additional hexaploids, two pentaploids, 2n = 40, and a septaploid, 2n = 56, were obtained.Chromosome associations were mostly bivalents, with a low frequency of univalents and quadrivalents in hexaploids with six sativa genomes or five sativa and one falcata genome. Pollen stainability was in excess of 95%, ovule number averaged 12 per carpel, and several grams of hexaploid seed were produced from hand crosses. Five different hexaploid synthetics with narrow and wide genetic bases were produced for future seed increase and field testing. The value of hexaploids in breeding and cytogenetic research was discussed.

scholarly journals The Antioxidant, Anti-Inflammatory, Pathological, and Behavioural Effects of Medicago sativa L. (Alfalfa) Extract on Brain Injury Caused by Nicotine in Male Rats

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
M. Raeeszadeh ◽  
P. Mortazavi ◽  
R. Atashin-Sadafi
Keyword(s):  
Medicago Sativa ◽  
Inflammatory Responses ◽  
The Other ◽  
Male Rats ◽  
Control Group ◽  
Elevated Plus Maze Test ◽  
Male Wistar Rats ◽  
Medicago Sativa L ◽  
Histopathological Studies ◽  
The Brain

Nicotine is one of the most important compounds in cigarette which can cause changes in the concentration of neurotransmitters and damage to the nervous system. The aim of this study was to investigate the effect of the hydroalcoholic extract of Medicago Sativa L. (alfalfa) on controlling nicotine-induced brain damage and anxiety behaviour in rats. Forty-two male Wistar rats were randomly divided into six equal groups and treated daily as follows: a control group, T1 and T2 groups where animals were subcutaneously injected 250 and 500 mg/kg alfalfa extract, respectively, T3 and T4 groups where animals were injected subcutaneously 0.2 mg/kg nicotine and 250 and 500 mg/kg alfalfa extract, and T5 group in which only nicotine at the dose of 0.2 mg/kg was injected. At the end of the period after weighing, the elevated plus-maze test was taken from the animals. Serum assay was conducted to measure TCA, IL-1, and TNFα, and half of the brain tissue was used to measure oxidative stress parameters (GPx, SOD, TAC, and MDA) and the other parts were used for histopathological studies. Body weight in the T5 group was significantly different from that of the other groups. The time and number of open arms reduced in the T5 group. The duration and number of times in the open arm significantly decreased in the treated groups in a dose-depended manner. Malondialdehyde concentration was the highest in the nicotine group and the lowest in T2. The concentration of GPx and SOD was significantly increased in the presence of alfalfa extract in nicotine groups. TNFα and IL-1 in the T5 group showed a significant increase compared to the other groups. Moreover, the number of neurons and the level of necrotic neurons and gliosis significantly decreased and increased in the nicotine group, respectively, while these histopathological damages improved by treatment with alfalfa extract in T3 and T4 groups. Alfalfa extract can have a significant dose-dependent therapeutic effect on inducing oxidative damage and inflammatory responses of nicotine in the brain and reducing anxiety behaviours.

Further studies on the determination of cold hardiness in Trifolium repens L. and Medicago sativa L.

1960 ◽  
Vol 11 (1) ◽  
pp. 1 ◽  
Author(s):  
CG Greenham ◽  
H Daday
Keyword(s):  
Medicago Sativa ◽  
Trifolium Repens ◽  
Cold Hardiness ◽  
Cold Treatment ◽  
Low Frequency ◽  
Resistance Index ◽  
Medicago Sativa L ◽  
Trifolium Repens L ◽  
Probe Measurements

This paper deals with probe measurements of low frequency resistance (RLF), high frequency resistance (RHF), and resistance index (= 100 x RLF/RHF), for the determination of cold hardiness or the injury caused by cold treatments. Populations of white clover (Trifolium repens L.) were subjected to different periods of freezing. Discrimination between treatments could be made in terms of RLF, RHF, and resistance index, though discrimination in terms of RHF is poor. The rest of the paper deals with lucerne (Medicago sativa L.). Measurements were made after a cold treatment on field plants consisting of four commercial strains and their F1 crosses, and also on F2 seedlings. RLF and the resistance index appear to be equally good predictors for the recovery of strains or of individual plants from such populations. The Appendix deals with measurements on commercial (tetraploid) plants, diploids, and colchicine-produced tetraploids.

THE BIOGENESIS OF ALKALOIDS: XVII. FURTHER STUDY OF THE ROLE OF ORNITHINE IN THE BIOGENESIS OF STACHYDRINE

1956 ◽  
Vol 34 (12) ◽  
pp. 1704-1708 ◽  
Author(s):  
Arthur Morgan ◽  
Léo Marion
Keyword(s):  
Amino Acids ◽  
Glutamic Acid ◽  
Medicago Sativa ◽  
Aspartic Acid ◽  
The Other ◽  
Feeding Experiment ◽  
Seed Formation ◽  
Keto Acids ◽  
Medicago Sativa L

Earlier attempts to feed ornithine-2-C14 to Medicago sativa L. Grimm had failed to produce any labelled stachydrine. Repetition of the work has confirmed this result, and a study of the amino acids present in the plant has shown that the proline also was inactive. Feeding pyridoxine with ornithine-2-C14 to the plant did not produce active stachydrine, but gave rise to radioactive proline. Of the other amino acids present, glycine, aspartic acid, and glutamic acid were also active. A similar feeding experiment with radioactive ornithine and pyridoxine, using older plants, also failed to produce active stachydrine although, as before, the proline was active and so was the keto acids fraction.The results seem to indicate that ornithine may be the precursor of stachydrine, but that the transformation to proline and the methylation to the alkaloid occur at a later stage of growth, possibly at seed formation.

RESPONSE OF SEEDS TO DRY HEAT

1977 ◽  
Vol 57 (1) ◽  
pp. 305-307 ◽  
Author(s):  
M. A. MAUN
Keyword(s):  
Medicago Sativa ◽  
Bromus Tectorum ◽  
Kentucky Bluegrass ◽  
The Other ◽  
Downy Brome ◽  
Barnyard Grass ◽  
Echinochloa Crusgalli ◽  
Dry Heat ◽  
Medicago Sativa L

The seeds of alfalfa (Medicago sativa L.) were more sensitive to heat than the other species tested. The germination of barnyard grass (Echinochloa crusgalli (L.) Beauv.) caryopses was significantly reduced by exposure for 1 h to temperatures of 100 C or over. Downy brome (Bromus tectorum L.) and Kentucky bluegrass could withstand temperatures of up to 120 C for 1 h without any loss in germination.

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