Towards developing intervarietal substitution lines in Brassica napus using marker-assisted selection

Genome ◽  
1996 ◽  
Vol 39 (2) ◽  
pp. 348-358 ◽  
Author(s):  
P. M. Howell ◽  
D. J. Lydiate ◽  
D. F. Marshall
Keyword(s):  
Brassica Napus ◽  
Marker Assisted Selection ◽  
General Strategy ◽  
Production Traits ◽  
Rigorous Analysis ◽  
Substitution Lines ◽  
Donor Genome ◽  
Selfed Progeny ◽  
Almost All ◽  
Intervarietal Substitution Lines

Sets of substitution lines have advantages over segregating populations for the rigorous analysis of loci influencing quantitative traits. A general strategy for the rapid production of substitution lines was developed. It involved the systematic application of marker-assisted selection over 2–4 generations of backcrossing. The effectiveness of this strategy was demonstrated by the production of intervarietal substitution lines in Brassica napus. A genetic map containing 158 loci, distributed across all 19 B. napus linkage groups and assayed in 200 B1 individuals, was generated. Six complementary B1 individuals enriched for recurrent genotype and collectively carrying almost all the donor genome were selected. A total of 288 B2 plants derived from the selected B1 individuals were analysed and complementary individuals carrying five or fewer donor segments were identified. Similar selection, carried out on 250 B3 plants from two distinct B1 lineages, identified 74 B3 individuals carrying one or two donor segments. Together, 12 of these isolated segments represented 33% of the mapped genome. Lines homozygous for single substituted segments were derived from selfed progeny of selected B3 plants. A full set of substitution lines will be used to elucidate the genetic control of quantitative production traits in oilseed rape over several environments. Key words : QTL mapping, quantitative genetics, backcross, genetic linkage map, plant breeding, restriction fragment length polymorphism.

scholarly journals Identification and evaluation of intervarietal substitution lines of rapeseed (Brassica napus L.) with donor segments affecting the diploidization rate of isolated microspores

Euphytica ◽  
2016 ◽  
Vol 209 (1) ◽  
pp. 181-198 ◽  
Author(s):  
Anthimos Kampouridis ◽  
Katharina Ziese-Kubon ◽  
Nurhasanah ◽  
Wolfgang Ecke
Keyword(s):  
Brassica Napus ◽  
Substitution Lines ◽  
Brassica Napus L ◽  
Isolated Microspores ◽  
Intervarietal Substitution Lines

scholarly journals Comparison on the Nutrient Plunder Capacity of Orychophragmus violaceus and Brassica napus L. Based on Electrophysiological Information

Horticulturae ◽  
2021 ◽  
Vol 7 (8) ◽  
pp. 206
Author(s):  
Cheng Zhang ◽  
Yue Su ◽  
Yanyou Wu ◽  
Haitao Li ◽  
Ying Zhou ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Orychophragmus Violaceus ◽  
Nutrient Metabolism ◽  
Brassica Napus L ◽  
Adaptive Mechanisms ◽  
Capacitive Reactance ◽  
Time Determination ◽  
Almost All ◽  
First Time

The nutrient metabolism, growth and development of plants are strongly affected by its nutrient plunder, and plants have different adaptive mechanisms to low-nutrient environments. The electrophysiological activities involve almost all life processes of plants. In this study, the active transport flow of nutrient (NAF) and nutrient plunder capacity (NPC) of plants were defined based on leaf intrinsic impedance (IZ), capacitive reactance (IXc), inductive reactance (IXL) and capacitance (IC) to evaluate the nutrient plunder capacity of plants for the first time. The results indicate that Orychophragmus violaceus had higher (p < 0.01) NPC and IC and lower (p < 0.01) IR, IXc, IXL and IZ as compared to Brassica napus L., which supports a superior ion affinity and that it could be better adapted to low-nutrient environments. UAF and NPC of plants exhibited good correlations with crude protein, crude ash and water content, and precisely revealed the plunder capacity and adaptive strategies of plants to nutrients. The present work highlights that O. violaceus had superior NPC and ion affinity compared with B. napus, and provided a novel, rapid, reliable method based on the plant’s electrophysiological information for real-time determination of the nutrient plunder capacity of plants.

scholarly journals Mapping of quantitative trait loci influencing daily body weight gain (DBWG) on chromosome 6 in German Holstein population

2006 ◽  
Vol 22 (1-2) ◽  
pp. 35-46 ◽  
Author(s):  
M. Reißmann ◽  
P. Reinecke ◽  
U. Müller ◽  
S. Abdel-Rahman
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Fine Mapping ◽  
Marker Assisted Selection ◽  
Growth Traits ◽  
Body Weight Gain ◽  
Chromosome 6 ◽  
Production Traits ◽  
Daily Body Weight ◽  
Holstein Population

Twelve microsatellite markers on chromosome 6 were analyzed in German Holstein population to detect and locate QTL affecting daily body weight gain (DBWG). The results indicate promising location for QTL controlling daily body weight gain trait on chromosome 6. Where, three markers BMS2508 BM3026 and TGLA37 at three different positions in a distance 15.2 cM on BTA6 were associated with significant effects for daily body weight gain trait (DBWG). Comparison between this finding and previously identified QTL support the location of a QTL for growth traits on chromosome 6, where a significant QTL for birth and yearling weight was previously identified on chromosome 6 tightly close to marker BM3026. Finding from this study could be used in subsequent fine-mapping work and applied to marker-assisted selection (MAS) of production traits.

scholarly journals QTL analysis of an intervarietal set of substitution lines in Brassica napus: (i) Seed oil content and fatty acid composition

Heredity ◽  
2003 ◽  
Vol 90 (1) ◽  
pp. 39-48 ◽  
Author(s):  
M J Burns ◽  
S R Barnes ◽  
J G Bowman ◽  
M H E Clarke ◽  
C P Werner ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Brassica Napus ◽  
Acid Composition ◽  
Qtl Analysis ◽  
Oil Content ◽  
Seed Oil ◽  
Seed Oil Content ◽  
Substitution Lines

scholarly journals Chromosomal effects on In Vitro morphogenesis in Wheat intervarietal Substitution Lines

2011 ◽  
Vol 42 (No. 1) ◽  
pp. 15-19 ◽  
Author(s):  
N.D. Tyankova ◽  
N. Zagorska ◽  
V. Chardakov ◽  
A. Dryanova ◽  
B. Dimitrov
Keyword(s):  
Chinese Spring ◽  
Regeneration Ability ◽  
Substitution Lines ◽  
In Vitro Morphogenesis ◽  
Chromosome Substitutions ◽  
Allele Variation ◽  
Two Parameters ◽  
Wheat Lines ◽  
Intervarietal Substitution Lines

The effect of individual chromosomes on in vitro morphogenic ability of immature embryos was studied using a Chinese Spring/Timstein substitution series. For this purpose 20 wheat lines of Chinese Spring with consequently substituted chromosome pairs from the cultivar Timstein together with the two parental cultivars were investigated. The regeneration ability of the studied lines was quantified by two parameters: frequency of regenerative calli and coefficient of propagation. The influence of the 5A, 1B and 4D chromosomes on in vitro morphogenesis was determined and the effect of 2A, 3A, 3B, 4B, 6B and 1D chromosomes, reported by other authors, was confirmed. The data indicated that the intervarietal substitutions of certain chromosomes caused significant variation in the number of morphogenic calli and recovered regenerants, in spite of the fact that the two parental cultivars were of similar morphogenic ability. This probably reflects the allele variation in a given locus on these chromosomes and/or background effect. The data suggest that the chromosome substitutions may have different compensatory effects depending on the similarity of the corresponding chromosomes and also on the possibilities of their expression in an alien genetic background. &nbsp;

scholarly journals Marker assisted selection of new high oleic and low linolenic winter oilseed rape (Brassica napus L.) inbred lines revealing good agricultural value

PLoS ONE ◽  
2020 ◽  
Vol 15 (6) ◽  
pp. e0233959 ◽  
Author(s):  
Stanisław Spasibionek ◽  
Katarzyna Mikołajczyk ◽  
Hanna Ćwiek–Kupczyńska ◽  
Teresa Piętka ◽  
Krystyna Krótka ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Marker Assisted Selection ◽  
Inbred Lines ◽  
Winter Oilseed Rape ◽  
Brassica Napus L ◽  
High Oleic ◽  
Low Linolenic ◽  
Selection Of

scholarly journals Using intervarietal substitution lines for the identification of wheat chromosomes involved in early responses to water-deficit stress

PLoS ONE ◽  
2019 ◽  
Vol 14 (8) ◽  
pp. e0221849
Author(s):  
Karolina Dudziak ◽  
Piotr Bulak ◽  
Magdalena Zapalska ◽  
Andreas Börner ◽  
Hubert Szczerba ◽  
...  
Keyword(s):  
Water Deficit ◽  
Water Deficit Stress ◽  
Substitution Lines ◽  
Intervarietal Substitution Lines
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