Relation of age of cultures to yield of mycelium, hydrogen cyanide production, peroxidases, and proteins from mycelium of a low-temperature basidiomycete

1968 ◽  
Vol 14 (11) ◽  
pp. 1169-1172 ◽  
Author(s):  
Awatar S. Sekhon ◽  
Nicholas Colotelo

Changes in dry weight, hydrogen cyanide production, peroxidase, and soluble proteins of mycelium of a low-temperature basidiomycete with age of cultures were studied.Hydrogen cyanide was detected only after there was a decrease in growth of mycelium as determined by dry-weight measurements. Polyacrylamide gel electrophoretic analyses showed that the pattern and numbers of peroxidase and other soluble-protein bands varied with the age of the culture. Concomitant with decreases in yield of mycelium, there was a decrease in the numbers of peroxidase and soluble-protein bands.

1985 ◽  
Vol 57 (3) ◽  
pp. 147-154
Author(s):  
Tuula Sontag ◽  
Hannu Salovaara ◽  
Osmo Ulvinen

The polyacrylamide gel electrophoretic (PAGE) patterns of soluble proteins and esterases of six Finnish potato cultivars (Jaakko, Pito, Hankkijan Timo, Hankkijan Tuomas, Hankkijan Tanu and Puikula) were determined. All cultivars are commonly grown in Finland. The PAGE procedure used yielded highly reproducible protein separation and good resolution. Samples studied had specific soluble protein and esterase PAGE patterns, indicating that electrophoregrams can be used for identifying Finnish potato cultivars. Only two cultivars, Hankkijan Tanu and Hankkijan Tuomas, which are close relatives, possessed very similar PAGE patterns. The electrophoretic pattern of Puikula was very similar to that of the Swedish cultivar Mandel when compared with the reference presented in the literature.Therefore a hypothesis is presented suggesting that these two local cultivars would be representatives of the same cultivar.


2019 ◽  
Vol 32 (1) ◽  
pp. 143-151 ◽  
Author(s):  
Luma Rayane de Lima Nunes ◽  
Paloma Rayane Pinheiro ◽  
Charles Lobo Pinheiro ◽  
Kelly Andressa Peres Lima ◽  
Alek Sandro Dutra

ABSTRACT Salinity is prejudicial to plant development, causing different types of damage to species, or even between genotypes of the same species, with the effects being aggravated when combined with other types of stress, such as heat stress. The aim of this study was to evaluate the tolerance of cowpea genotypes (Vigna unguiculata L. Walp.) to salt stress at different temperatures. Seeds of the Pujante, Epace 10 and Marataoã genotypes were placed on paper rolls (Germitest®) moistened with different salt concentrations of 0.0 (control), 1.5, 3.0, 4.5 and 6.0 dS m-1, and placed in a germination chamber (BOD) at temperatures of 20, 25, 30 and 35°C. The experiment was conducted in a completely randomised design, in a 3 × 4 × 5 scheme of subdivided plots, with four replications per treatment. The variables under analysis were germination percentage, first germination count, shoot and root length, and total seedling dry weight. At temperatures of 30 and 35°C, increases in the salt concentration were more damaging to germination in the Epace 10 and Pujante genotypes, while for the Marataoã genotype, damage occurred at the temperature of 20°C. At 25°C, germination and vigour in the genotypes were higher, with the Pujante genotype proving to be more tolerant to salt stress, whereas Epace 10 and Marataoã were more tolerant to high temperatures. Germination in the cowpea genotypes was more sensitive to salt stress when subjected to heat stress caused by the low temperature of 20°C or high temperature of 35°C.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Adam Yousif Adam Ali ◽  
Muhi Eldeen Hussien Ibrahim ◽  
Guisheng Zhou ◽  
Nimir Eltyb Ahmed Nimir ◽  
Aboagla Mohammed Ibrahim Elsiddig ◽  
...  

AbstractSalinity one of environmental factor that limits the growth and productivity of crops. This research was done to investigate whether GA3 (0, 144.3, 288.7 and 577.5 μM) and nitrogen fertilizer (0, 90 and 135 kg N ha−1) could mitigate the negative impacts of NaCl (0, 100, and 200 mM NaCl) on emergence percentage, seedling growth and some biochemical parameters. The results showed that high salinity level decreased emergence percentage, seedling growth, relative water content, chlorophyll content (SPAD reading), catalase (CAT) and peroxide (POD), but increased soluble protein content, superoxide dismutase (SOD) activity and malondialdehyde (MDA) content. The SOD activity was decreased by nitrogen. However, the other measurements were increased by nitrogen. The interactive impact between nitrogen and salinity was significant in most parameters except EP, CAT and POD. The seedling length, dry weight, fresh weight, emergence percentage, POD, soluble protein and chlorophyll content were significantly affected by the interaction between GA3 and salinity. The GA3 and nitrogen application was successful mitigating the adverse effects of salinity. The level of 144.3 and 288.7 μm GA3 and the rate of 90 and 135 kg N ha−1 were most effective on many of the attributes studied. Our study suggested that GA3 and nitrogen could efficiently protect early seedlings growth from salinity damage.


2017 ◽  
Vol 3 (3) ◽  
pp. 157
Author(s):  
M. Furutani ◽  
Y. Ohta ◽  
M. Nose

<p>Flat low-temperature two-stage flames were established on a Powling burner using rich diethyl-ether/ air or n-heptane/air mixtures, and nitrogen monoxide NO was added into the fuel-air mixtures with a concentration of 240 ppm. The temperature development and chemical-species histories, especially of NO, nitrogen dioxide NO<sub>2</sub> and hydrogen cyanide HCN were examined associated with an emission-spectrum measurement from the low-temperature flames. Nitrogen monoxide was consumed in the cool-flame region, where NO was converted to the NO<sub>2</sub>. The NO<sub>2</sub> generated, however, fell suddenly in the cool-flame degenerate region, in which the HCN superseded. In the blue-flame region the NO came out again and developed accompanied with remained HCN in the post blue-flame region. The NO seeding into the mixture intensified the blue-flame luminescence probably due to the cyanide increase.</p>


Weed Science ◽  
1970 ◽  
Vol 18 (5) ◽  
pp. 565-571
Author(s):  
J. A. Mulliken ◽  
C. A. Kust ◽  
L. E. Schrader

Endosperm dry weight, protein, and fat losses accompanied rapid radicle growth of velvetleaf (Abutilon theophrasti Medic.) between 12 and 36 hr of germination at 31 C. Cotyledonary reserves were mobilized after 36 hr. Isocitrate lyase activity sedimented with a particulate fraction in varying degrees, but maximal activity developed at times coincident with fat mobilization. Respiration of excised endosperms reached maximal rates shortly after radicle emergence. The actions of hydrogen cyanide, carbon monoxide, and 2,4-dinitrolphenol indicated that respiration of endosperms excised from imbibed and germinated seed was due to cytochrome oxidase activity, and was coupled to phosphorylation.


2011 ◽  
Vol 57 (No. 10) ◽  
pp. 459-464 ◽  
Author(s):  
Q.S. Wu

Citrus plants often suffer from temperature stress, which seriously inhibits tree growth and even results in tree death. The present experiment was conducted to evaluate the effects of Glomus mosseae on growth, root morphology, superoxide dismutase (SOD) and catalase (CAT) activities, and soluble protein content of trifoliate orange (Poncirus trifoliata) seedlings at low (15&deg;C), optimum (25&deg;C) and high (35&deg;C) temperatures. Sixty-eight days after temperature stresses, mycorrhizal colonization and number of both entry points and vesicles were significantly inhibited by low or high temperature. Mycorrhizal seedlings recorded significantly higher growth characteristics than non-mycorrhizal seedlings at both optimum and high temperatures, but the beneficial effects were almost lost at low temperature. Generally, mycorrhizal seedlings presented notably higher root traits (projected area, surface area, number of forks and volume) than non-mycorrhizal seedlings regardless of temperature levels. Mycorrhizal colonization significantly increased SOD and CAT activities and soluble protein content at high temperature, increased only SOD activity at optimum temperature, and decreased only soluble protein content at low temperature. It suggests that mycorrhizal alleviation of temperature stress in trifoliate orange seedlings was at high temperature, but the alleviation was obviously weakened at low temperature.


2019 ◽  
Vol 36 (9) ◽  
pp. 1011-1018
Author(s):  
Fei Wang ◽  
You Lv ◽  
Wang Xueqian ◽  
Yixing Ma ◽  
Ping Ning

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