Behavior of colorless appressoria in an albino mutant of Colletotrichum lagenarium

1982 ◽  
Vol 28 (11) ◽  
pp. 1210-1213 ◽  
Author(s):  
K. Suzuki ◽  
Y. Kubo ◽  
I. Furusawa ◽  
N. Ishida ◽  
M. Yamamoto
Keyword(s):  
Nuclear Division ◽  
Temperature Sensitive ◽  
Colletotrichum Lagenarium ◽  
Albino Mutant ◽  
And Migration ◽  
Appressoria Formation

Spores of an albino mutant of Colletotrichum lagenarium formed colorless appressoria. The appressoria germinated laterally and formed secondary appressoria after further incubation. Appressorial formation was accompanied by nuclear division and migration of the spore contents into the primary appressorium. When the primary appressorium was mature, the nucleus in the spore had disappeared and the spore appeared empty. Similar phenomena were observed in the formation of secondary appressoria from primary appressoria. Formation of both primary and secondary appressoria was temperature sensitive. When spores were incubated in the presence of cycloheximide after 1 h of incubation, formation of primary and secondary appressoria was not affected. Secondary appressoria pigmented by addition of 3,4-dihydroxyphenylalanine could penetrate nitrocellulose membranes.

scholarly journals A Screen for Genes Involved in the Anaphase Proteolytic Pathway Identifies tsm1+, a Novel Schizosaccharomyces pombe Gene Important for Microtubule Integrity

Genetics ◽  
1998 ◽  
Vol 149 (3) ◽  
pp. 1251-1264
Author(s):  
Ekaterina L Grishchuk ◽  
James L Howe ◽  
J Richard McIntosh
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Nuclear Division ◽  
Mitotic Division ◽  
Temperature Sensitive ◽  
Permissive Temperature ◽  
Anaphase Promoting Complex ◽  
Chloromethyl Ketone ◽  
Proteolytic Pathway ◽  
Functional Involvement ◽  
Spindle Elongation

Abstract The growth of several mitotic mutants of Schizosaccharomyces pombe, including nuc2-663, is inhibited by the protease inhibitor N-Tosyl-L-Phenylalanine Chloromethyl Ketone (TPCK). Because nuc2+ encodes a presumptive component of the Anaphase Promoting Complex, which is required for the ubiquitin-dependent proteolysis of certain proteins during exit from mitosis, we have used sensitivity to TPCK as a criterion by which to search for novel S. pombe mutants defective in the anaphase-promoting pathway. In a genetic screen for temperature-sensitive mitotic mutants that were also sensitive to TPCK at a permissive temperature, we isolated three tsm (TPCK-sensitive mitotic) strains. Two of these are alleles of cut1+, but tsm1-512 maps to a novel genetic location. The tsm1-512 mutation leads to delayed nuclear division at restrictive temperatures, apparently as a result of an impaired ability to form a metaphase spindle. After shift of early G2 cells to 36°, tsm1-512 arrests transiently in the second mitotic division and then exits mitosis, as judged by spindle elongation and septation. The chromosomes, however, often fail to segregate properly. Genetic interactions between tsm1-512 and components of the anaphase proteolytic pathway suggest a functional involvement of the Tsm1 protein in this pathway.

Relation between pigment intensity and penetrating ability in appressoria of Colletotrichum lagenarium

1987 ◽  
Vol 33 (10) ◽  
pp. 870-873 ◽  
Author(s):  
Yasuyuki Kubo ◽  
Iwao Furusawa ◽  
Jiko Shishiyama
Keyword(s):  
Parent Strain ◽  
Colletotrichum Lagenarium ◽  
Melanin Biosynthesis ◽  
Glass Slides ◽  
Albino Mutant

Mutant 8004 of Colletotrichum lagenarium formed slightly pigmented appressoria. The appressoria germinated laterally on glass slides, but could penetrate nitrocellulose membranes. The slightly pigmented appressoria of the albino mutant 79215 were observed to behave in a similar manner when treated with 100 μM scytalone. The appressorial pigmentation of mutant 8004 was increased by the application of scytalone, and mature appressorial pigmentation, indistinguishable from that of the parent strain 104-T, was observed when appressoria were treated with 100 μM scytalone, a lower concentration than that needed for mature pigmentation of the albino mutant. The sensitivity to tricyclazole of appressorial pigmentation of mutant 8004 was higher than that of the parent strain 104-T. These results suggest that, although melanin biosynthesis is essential for appressorial penetration, slight pigmentation is sufficient.

scholarly journals The Highly Conserved tRNAHis Guanylyltransferase Thg1p Interacts with the Origin Recognition Complex and Is Required for the G2/M Phase Transition in the Yeast Saccharomyces cerevisiae

2005 ◽  
Vol 4 (4) ◽  
pp. 832-835 ◽  
Author(s):  
Terri S. Rice ◽  
Min Ding ◽  
David S. Pederson ◽  
Nicholas H. Heintz
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Origin Recognition Complex ◽  
Nuclear Division ◽  
Permissive Temperature ◽  
Yeast Saccharomyces Cerevisiae ◽  
M Phase ◽  
And Migration ◽  
Origin Recognition

ABSTRACT Here we show that the Saccharomyces cerevisiae tRNAHis guanylyltransferase Thg1p interacts with the origin recognition complex in vivo and in vitro and that overexpression of hemagglutinin-Thg1p selectively impedes growth of orc2-1(Ts) cells at the permissive temperature. Studies with conditional mutants indicate that Thg1p couples nuclear division and migration to cell budding and cytokinesis in yeast.

Biochemical and molecular analysis of a temperature-sensitive albino mutant in kale named “White Dove”

2013 ◽  
Vol 71 (3) ◽  
pp. 281-294 ◽  
Author(s):  
Shuang Zhou ◽  
Zongli Hu ◽  
Mingku Zhu ◽  
Bin Zhang ◽  
Lei Deng ◽  
...  
Keyword(s):  
Molecular Analysis ◽  
Temperature Sensitive ◽  
Albino Mutant

scholarly journals Overproduction of discoidin I by a temperature-sensitive motility mutant of Dictyostelium discoideum.

1984 ◽  
Vol 4 (6) ◽  
pp. 1035-1041 ◽  
Author(s):  
S Biswas ◽  
S C Kayman ◽  
M Clarke
Keyword(s):  
Dictyostelium Discoideum ◽  
Gel Electrophoresis ◽  
Peptide Mapping ◽  
Temperature Sensitive ◽  
Restrictive Temperature ◽  
Axenic Medium ◽  
Two Dimensional Gel Electrophoresis ◽  
Motility Mutant ◽  
And Migration ◽  
Mutant Cells

Dictyostelium discoideum MC2 is a temperature-sensitive motility mutant of AX3. Mutant cells are incapable of growth, phagocytosis, and migration under restrictive conditions (Kayman et al., J. Cell Biol. 92:705-711, 1982). We show here that at the restrictive temperature MC2 cells grown axenically or on bacteria synthesized excessive quantities of the lectin discoidin I. By two-dimensional gel electrophoresis and peptide mapping, the proteins overproduced by MC2 cells were indistinguishable from discoidin I synthesized at lower levels in AX3 cells. At least two of the three species of discoidin I were overproduced. This protein family constituted 9% of the total protein in cells that were incubated overnight at 27 degrees C in axenic medium. Although MC2 cells are defective in nutrient uptake under restrictive conditions, the overproduction of discoidin I did not appear to be part of a pleiotropic response to starvation. We propose that transcription of the coordinately regulated discoidin I genes is altered in mutant cells. This alteration may be related to the motility defects manifested by MC2.

scholarly journals A novel histone H4 mutant defective in nuclear division and mitotic chromosome transmission.

1996 ◽  
Vol 16 (3) ◽  
pp. 1017-1026 ◽  
Author(s):  
M M Smith ◽  
P Yang ◽  
M S Santisteban ◽  
P W Boone ◽  
A T Goldstein ◽  
...  
Keyword(s):  
Mitotic Chromosome ◽  
Nuclear Division ◽  
Dna Recombination ◽  
Chromosome Loss ◽  
Temperature Sensitive ◽  
Restrictive Temperature ◽  
Histone H4 ◽  
Eukaryotic Chromosome ◽  
Chromosome Transmission

The histone proteins are essential for the assembly and function of th e eukaryotic chromosome. Here we report the first isolation of a temperature-sensitive lethal histone H4 mutant defective in mitotic chromosome transmission Saccharomyces cerevisiae. The mutant requires two amino acid substitutions in histone H4: a lethal Thr-to-Ile change at position 82, which lies within one of the DNA-binding surfaces of the protein, and a substitution of Ala to Val at position 89 that is an intragenic suppressor. Genetic and biochemical evidence shows that the mutant histone H4 is temperature sensitive for function but not for synthesis, deposition, or stability. The chromatin structure of 2 micrometer circle minichromosomes is temperature sensitive in vivo, consistent with a defect in H4-DNA interactions. The mutant also has defects in transcription, displaying weak Spt- phenotypes. At the restrictive temperature, mutant cells arrest in the cell cycle at nuclear division, with a large bud, a single nucleus with 2C DNA content, and a short bipolar spindle. At semipermissive temperatures, the frequency of chromosome loss is elevated 60-fold in the mutant while DNA recombination frequencies are unaffected. High-copy CSE4, encoding an H3 variant related to the mammalian CENP-A kinetochore antigen, was found to suppress the temperature sensitivity of the mutant without suppressing the Spt- transcription defect. These genetic, biochemical, and phenotypic results indicate that this novel histone H4 mutant defines one or more chromatin-dependent steps in chromosome segregation.

scholarly journals Nuf2, a spindle pole body-associated protein required for nuclear division in yeast.

1994 ◽  
Vol 125 (4) ◽  
pp. 853-866 ◽  
Author(s):  
M A Osborne ◽  
G Schlenstedt ◽  
T Jinks ◽  
P A Silver
Keyword(s):  
Nuclear Division ◽  
Spindle Pole Body ◽  
Coiled Coil ◽  
Temperature Shift ◽  
Spindle Pole ◽  
Temperature Sensitive ◽  
Yeast Saccharomyces Cerevisiae ◽  
Culture Cells ◽  
Pole Body ◽  
Associated Proteins

The NUF2 gene of the yeast Saccharomyces cerevisiae encodes an essential 53-kd protein with a high content of potential coiled-coil structure similar to myosin. Nuf2 is associated with the spindle pole body (SPB) as determined by coimmunofluorescence with known SPB proteins. Nuf2 appears to be localized to the intranuclear region and is a candidate for a protein involved in SPB separation. The nuclear association of Nuf2 can be disrupted, in part, by 1 M salt but not by the detergent Triton X-100. All Nuf2 can be removed from nuclei by 8 M urea extraction. In this regard, Nuf2 is similar to other SPB-associated proteins including Nufl/SPC110, also a coiled-coil protein. Temperature-sensitive alleles of NUF2 were generated within the coiled-coil region of Nuf2 and such NUF2 mutant cells rapidly arrest after temperature shift with a single undivided or partially divided nucleus in the bud neck, a shortened mitotic spindle and their DNA fully replicated. In sum, Nuf2 is a protein associated with the SPB that is critical for nuclear division. Anti-Nuf2 antibodies also recognize a mammalian 73-kd protein and display centrosome staining of mammalian tissue culture cells suggesting the presence of a protein with similar function.

scholarly journals AN ENDOMITOTIC EFFECT OF A CELL CYCLE MUTATION OF SACCHAROMYCES CEREVISIAE

Genetics ◽  
1981 ◽  
Vol 97 (3-4) ◽  
pp. 551-562 ◽  
Author(s):  
David Schild ◽  
Honnavara N Ananthaswamy ◽  
Robert K Mortimer
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Genetic Analysis ◽  
Nuclear Division ◽  
Pedigree Analysis ◽  
Visual Observation ◽  
Temperature Sensitive ◽  
Cell Stage ◽  
Haploid Strain ◽  
A Cell ◽  
Dna Measurements

ABSTRACT A recessive temperature-sensitive mutation of Saccharomyces cerevisiae has been isolated and shown to cause an increase in ploidy in both haploids and diploids. Genetic analysis revealed that the strain carrying the mutation was an aa diploid, although MNNG mutagenesis had been done on an a haploid strain. When the mutant strain was crossed with an aa diploid and the resultant tetraploid sporulated, some of the meiotic progeny of this tetraploid were themselves tetraploid, as shown by both genetic analysis and DNA measurements, instead of diploid as expected of tetraploid meiosis. The ability of these tetraploids to continue to produce tetraploid meiotic progeny was followed for four generations. Homothallism was excluded as a cause of the increase in ploidy; visual pedigree analysis of spore clones to about the 32-cell stage failed to reveal any zygotes, and haploids that diploidized retained their mating type. An extra round of meiotic DNA synthesis was also considered and excluded. It was found that tetraploidization was independent of sporulation temperature, but was dependent on the temperature of germination and the growth of the spores. Increase in ploidy occurred when the spores were germinated and grown at 30°, but did not occur at 23°. Two cycles of sporulation and growth at 23° resulted in haploids, which were shown to diploidize within 24 hr when grown at 30°. Visual observation of the haploid cells incubated at 36° revealed a celldivisioncycle phenotype characteristic of mutations that affect nuclear division; complementation analysis demonstrated that the mutation, cdc31-2, is allelic to cdc31-1, a mutation isolated by HARTWEeLL et al.(1973) and characterized as causing a temperature-sensitive arrest during late nuclear division. The segregation of cdc31-2 in heterozygous diploids was 2:2 and characteristic of a noncentromere-linked gene.

Phenotypic identification and gene mapping of temperature-sensitive green-revertible albino mutant tsa2 in rice (Oryza sativa L.)

2019 ◽  
Vol 45 (5) ◽  
pp. 662
Author(s):  
Li-Na SHANG ◽  
Xin-Long CHEN ◽  
Sheng-Nan MI ◽  
Gang WEI ◽  
Ling WANG ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Gene Mapping ◽  
Oryza Sativa L ◽  
Temperature Sensitive ◽  
Phenotypic Identification ◽  
Albino Mutant

Septum formation in Aspergillus nidulans

1995 ◽  
Vol 73 (S1) ◽  
pp. 396-399 ◽  
Author(s):  
Michelle Momany ◽  
Jennifer L. Morrell ◽  
Steven D. Harris ◽  
John E. Hamer
Keyword(s):  
Aspergillus Nidulans ◽  
Antibody Production ◽  
Nuclear Division ◽  
Temperature Sensitive ◽  
Cellular Processes ◽  
The Third ◽  
Septum Formation ◽  
Chromosome Transmission ◽  
Insight Into

We are investigating septation in Aspergillus nidulans. We have shown that septum formation is dependent on the third nuclear division and actin is involved in this process. We have also characterized nine temperature-sensitive septation (sep) mutants. On the basis of our analysis we have divided these mutants into three phenotypic classes. We are uncovering the order of events in the septation pathway by analysis of double mutants constructed with different pairs of sep mutants. The sepB gene has been cloned and sequenced. Homology with the Saccharomyces cerevisiae CTF4 gene and the phenotype of the sepB mutant support a role in monitoring the fidelity of chromosome transmission. We are also investigating the role of the asp genes (Aspergillus septins). Three asp genes were identified by homology with the S. cerevisiae septins. aspB has been cloned, sequenced, and fused to a biotinylated tag for antibody production. Antibody production and localization studies are now underway. Because septation requires the integration of several cellular processes, our studies should give insight into the cell cycle, cell wall biosnythesis and development of A. nidulans. Key words: septation, cytokinesis, Aspergillus nidulans.

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