A lipopolysaccharide-specific bacteriophage for Aeromonas salmonicida

1983 ◽  
Vol 29 (10) ◽  
pp. 1458-1461 ◽  
Author(s):  
E. E. Ishiguro ◽  
Teresa Ainsworth ◽  
D. H. Shaw ◽  
W. W. Kay ◽  
T. J. Trust
Keyword(s):  
Cell Wall ◽  
Lipid A ◽  
Aeromonas Salmonicida ◽  
Receptor Activity ◽  
Core Oligosaccharide ◽  
The Core ◽  
Phage Adsorption ◽  
Specific Bacteriophage

Cell wall lipopolysaccharide (LPS) was identified as the receptor for the Aeromonas salmonicida bacteriophage strain 55R-1. Mutants of A. salmonicida resistant to phage 55R-1 were unable to adsorb phage 55R-1 and were shown to be defective in LPS structure. Purified A. salmonicida LPS inactivated phage 55R-1, but the O-polysaccharide and the core oligosaccharide portions of the LPS were ineffective. These results suggest that lipid A was required for receptor activity. Antibodies directed against LPS also inhibited phage adsorption.

scholarly journals The Activity of Lipid A and Core Components of Bacterial Lipopolysaccharides in the Prevention of the Hypersensitive Response in Pepper

1997 ◽  
Vol 10 (7) ◽  
pp. 926-928 ◽  
Author(s):  
Mari-Anne Newman ◽  
Michael J. Daniels ◽  
J. Maxwell Dow
Keyword(s):  
Hypersensitive Response ◽  
Xanthomonas Campestris ◽  
Lipid A ◽  
Enteric Bacteria ◽  
Core Oligosaccharide ◽  
The Core ◽  
Minimal Structure ◽  
Core Components ◽  
Pre Treatment ◽  
Bacterial Lipopolysaccharides

Pre-treatment of leaves of pepper (Capsicum annuum) with lipopolysaccharide (LPS) preparations from enteric bacteria and Xanthomonas campestris could prevent the hypersensitive response caused by an avirulent X. campestris strain. By use of a range of deep-rough mutants, the minimal structure in Salmonella LPS responsible for the elicitation of this effect was determined to be lipid A attached to a disaccharide of 2-keto-3-deoxyoctulosonate; lipid A alone and the free core oligosaccharide from a Salmonella Ra mutant were not effective. For Xanthomonas, the core oligosaccharide alone had activity although lipid A was not effective. The results suggest that pepper cells can recognize different structures within bacterial LPS to trigger alterations in plant response to avirulent pathogens.

scholarly journals Interactions of Pulmonary Collectins with Bordetella bronchiseptica and Bordetella pertussis Lipopolysaccharide Elucidate the Structural Basis of Their Antimicrobial Activities

2004 ◽  
Vol 72 (12) ◽  
pp. 7124-7130 ◽  
Author(s):  
Lyndsay M. Schaeffer ◽  
Francis X. McCormack ◽  
Huixing Wu ◽  
Alison A. Weiss
Keyword(s):  
Bordetella Pertussis ◽  
Lipid A ◽  
Antimicrobial Activities ◽  
Innate Immune ◽  
Bordetella Bronchiseptica ◽  
Structural Basis ◽  
Wild Type ◽  
Core Oligosaccharide ◽  
The Core ◽  
O Antigen

ABSTRACT Surfactant proteins A (SP-A) and D (SP-D) play an important role in the innate immune defenses of the respiratory tract. SP-A binds to the lipid A region of lipopolysaccharide (LPS), and SP-D binds to the core oligosaccharide region. Both proteins induce aggregation, act as opsonins for neutrophils and macrophages, and have direct antimicrobial activity. Bordetella pertussis LPS has a branched core structure and a nonrepeating terminal trisaccharide. Bordetella bronchiseptica LPS has the same structure, but lipid A is palmitoylated and there is a repeating O-antigen polysaccharide. The ability of SP-A and SP-D to agglutinate and permeabilize wild-type and LPS mutants of B. pertussis and B. bronchiseptica was examined. Previously, wild-type B. pertussis was shown to resist the effects of SP-A; however, LPS mutants lacking the terminal trisaccharide were susceptible to SP-A. In this study, SP-A was found to aggregate and permeabilize a B. bronchiseptica mutant lacking the terminal trisaccharide, while wild-type B. bronchiseptica and mutants lacking only the palmitoyl transferase or O antigen were resistant to SP-A. Wild-type B. pertussis and B. bronchiseptica were both resistant to SP-D; however, LPS mutants of either strain lacking the terminal trisaccharide were aggregated and permeabilized by SP-D. We conclude that the terminal trisaccharide protects Bordetella species from the bactericidal functions of SP-A and SP-D. The O antigen and palmitoylated lipid A of B. bronchiseptica play no role in this resistance.

Complete Lipopolysaccharide ofPlesiomonas shigelloidesO74:H5 (Strain CNCTC 144/92). 2. Lipid A, Its Structural Variability, the Linkage to the Core Oligosaccharide, and the Biological Activity of the Lipopolysaccharide†,‡

Biochemistry ◽  
2006 ◽  
Vol 45 (35) ◽  
pp. 10434-10447 ◽  
Author(s):  
Jolanta Lukasiewicz ◽  
Monika Dzieciatkowska ◽  
Tomasz Niedziela ◽  
Wojciech Jachymek ◽  
Anna Augustyniuk ◽  
...  
Keyword(s):  
Biological Activity ◽  
Lipid A ◽  
Core Oligosaccharide ◽  
The Core ◽  
Structural Variability

Lipopolysaccharides of the motile aeromonads; core oligosaccharide analysis as an aid to taxonomic classification

1978 ◽  
Vol 24 (7) ◽  
pp. 864-868 ◽  
Author(s):  
Derek H. Shaw ◽  
H. J. Hodder
Keyword(s):  
Cell Wall ◽  
Numerical Analysis ◽  
Core Region ◽  
Taxonomic Classification ◽  
Core Oligosaccharide ◽  
The Core ◽  
Computer Based

Twelve motile Aeromonas strains have been examined with respect to the hexose and heptose monosaccharide residues present in the core region of their cell wall lipopolysaccharides. These strains were divided into three distinctly separate groups on the basis of the various combinations of hexose and heptose residues. The assignment of a strain to any one of the three groups furnishes a distribution which is substantially the same as that recently reported in a computer-based numerical analysis. All strains tested which were previously named A. liquefaciens fall into the same group.

The core oligosaccharide component from Mannheimia (Pasteurella) haemolytica serotype Al lipopolysaccharide contains L-glycero-D-manno- and D-glycero-D-manno-heptoses: Analysis of the structure and conformation by high-resolution NMR spectroscopy

2002 ◽  
Vol 80 (8) ◽  
pp. 949-963 ◽  
Author(s):  
Jean-Robert Brisson ◽  
Ellen Crawford ◽  
Dušan Uhrín ◽  
Nam Huan Khieu ◽  
Malcolm B Perry ◽  
...  
Keyword(s):  
Lipid A ◽  
Inner Core ◽  
Chemical Shifts ◽  
Coupling Constants ◽  
Pasteurella Haemolytica ◽  
Core Oligosaccharide ◽  
The Core ◽  
Proton Coupling ◽  
Structure Formula ◽  
Nmr Methods

Previous studies from our laboratory have indicated that the lipopolysaccharide (LPS) from Mannheimia haemolytica serotype A1 contains both L-glycero-D-manno-heptose and D-glycero-D-manno-heptose residues. NMR methods making use of 1D 1H selective excitation and 2D (1H, 13C) and (1H, 31P) heteronuclear experiments were used for the structural determination of the major core oligosaccharide components of the deacylated low-molecular-mass LPS obtained following sequential treatment with anhydrous hydrazine and aq KOH. The core oligosaccharide region was found to be composed of a branched octasaccharide linked to the deacylated lipid A moiety via a 3-deoxy-4-phospho-D-manno-oct-2-ulosonate residue having the structure,[Formula: see text]Heterogeneity was found to be present at several linkages. NMR methods were devised to distinguish between the diastereomeric forms of the heptose residues. Synthesized monosaccharides of L-D- and D-D-heptose were used as model compounds for analysis of the 1H and 13C NMR chemical shifts and proton coupling constants. Molecular modeling using a Monte Carlo method for conformational analysis of saccharides was used to determine the conformation of the inner core of the oligosaccharide and to establish the stereochemical relationships between the heptoses.Key words: LPS, NMR, conformation, oligosaccharide, heptose.

The Chemical Composition and Serological Reactions of Lipopolysaccharides from Serogroups A, B, X, and Y Neisseria meningitidis

1973 ◽  
Vol 51 (10) ◽  
pp. 1347-1354 ◽  
Author(s):  
H. J. Jennings ◽  
G. B. Hawes ◽  
G. A. Adams ◽  
C. P. Kenny
Keyword(s):  
Fatty Acids ◽  
Cell Wall ◽  
Sialic Acid ◽  
Chemical Composition ◽  
Neisseria Meningitidis ◽  
Main Part ◽  
Lipid A ◽  
The Core ◽  
Serological Studies ◽  
The Individual

Analyses of the cell wall lipopolysaccharides prepared from Neisseria meningitidis serogroups A, B, X, and Y, indicated that they all contained glucose, galactose, glucosamine, heptose, lipid A, ethanolamine, fatty acids, phosphate, and protein. Some minor compositional differences in the sugar components did occur in that sialic acid (6–7%) was found only in serogroups B and Y, and galactosamine (2–3%) in serogroups B and X. The individual fatty acid and amino acid components in the four serogroups were also qualitatively similar. The Neisseria lipopolysaccharides studied have the same components as the core structure of the Enterobacterioceae but appear to lack the characteristic components of their O-antigen side chains. Serological studies indicated that the lipopolysaccharides were homogeneous and were for the main part group specific in nature, despite their compositional similarity. On the basis of compositional differences alone it is not possible to account for the group specificity that they exhibit.

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