Glucose metabolism and pyruvate excretion by Streptomyces alboniger

1985 ◽  
Vol 31 (8) ◽  
pp. 702-706 ◽  
Author(s):  
Kenneth G. Surowitz ◽  
Robert M. Pfister
Keyword(s):  
Carbon Source ◽  
High Performance ◽  
Sole Carbon Source ◽  
Tricarboxylic Acid Cycle ◽  
Carbon Sources ◽  
Aerial Mycelium ◽  
Tricarboxylic Acid ◽  
Acid Metabolite ◽  
Acid Cycle ◽  
Glycolytic Activity

The formation of aerial mycelia and spores by Streptomyces alboniger has been observed to be inhibited by glucose supplied in the growth medium as the sole carbon source or supplied in combination with other utilizable carbon sources. Analysis of the metabolism of radiolabelled mannose and sucrose in the presence and absence of glucose demonstrated that glucose functions as the preferred carbon source, inhibiting the uptake and oxidation of the sugars within 15 min of its addition. The inhibition of aerial mycelium formation was shown to result from the excretion of an acidic metabolite, and could be overcome by the addition of a buffering system. The acid metabolite was identified as pyruvic acid by high-performance liquid chromatography and by paper chromatography. Acid was not produced in substantial quantities in dextrin broth or in glucose broth supplemented with 5 mM adenine. Analysis of the pathway of pyruvate overproduction demonstrated that growth on glucose resulted in increased glycolytic activity, relative to the activity of the tricarboxylic acid cycle on this substrate, while growth on dextrin or glucose supplemented with 5 mM adenine resulted in balanced glycolytic and tricarboxylic acid cycle activities.

scholarly journals Characteristics of alanine: glyoxylate aminotransferase from Saccharomyces cerevisiae, a regulatory enzyme in the glyoxylate pathway of glycine and serine biosynthesis from tricarboxylic acid-cycle intermediates

1985 ◽  
Vol 231 (1) ◽  
pp. 157-163 ◽  
Author(s):  
Y Takada ◽  
T Noguchi
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Tricarboxylic Acid Cycle ◽  
Tricarboxylic Acid ◽  
Acid Cycle ◽  
Tricarboxylic Acid Cycle Intermediates ◽  
Glyoxylate Aminotransferase ◽  
Serine Biosynthesis ◽  
Glyoxylate Pathway

Alanine: glyoxylate aminotransferase (EC 2.6.1.44), which is involved in the glyoxylate pathway of glycine and serine biosynthesis from tricarboxylic acid-cycle intermediates in Saccharomyces cerevisiae, was highly purified and characterized. The enzyme had Mr about 80 000, with two identical subunits. It was highly specific for L-alanine and glyoxylate and contained pyridoxal 5′-phosphate as cofactor. The apparent Km values were 2.1 mM and 0.7 mM for L-alanine and glyoxylate respectively. The activity was low (10 nmol/min per mg of protein) with glucose as sole carbon source, but was remarkably high with ethanol or acetate as carbon source (930 and 430 nmol/min per mg respectively). The transamination of glyoxylate is mainly catalysed by this enzyme in ethanol-grown cells. When glucose-grown cells were incubated in medium containing ethanol as sole carbon source, the activity markedly increased, and the increase was completely blocked by cycloheximide, suggesting that the enzyme is synthesized de novo during the incubation period. Similarity in the amino acid composition was observed, but immunological cross-reactivity was not observed among alanine: glyoxylate aminotransferases from yeast and vertebrate liver.

Utilization of fructose and glutamate by Rhodospirillum rubrum

1968 ◽  
Vol 14 (5) ◽  
pp. 493-498 ◽  
Author(s):  
Margaret S. Gibson ◽  
Chih H. Wang
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
Rhodospirillum Rubrum ◽  
Tricarboxylic Acid Cycle ◽  
Tricarboxylic Acid ◽  
Glycolytic Pathway ◽  
Light Conditions ◽  
Acid Cycle

Fructose served as sole carbon source for the growth of Rhodospirillum rubrum anaerobically under light or aerobically in the dark while glucose did not. Glucose was not utilized by the organism at all. Radiorespirometric studies, using 14C specifically labelled fructose as substrate, revealed that fructose is catabolized exclusively via the Embden–Meyerhof–Parnas (EMP) glycolytic pathway. Both L-glutamic and D-glutamic acids can be utilized by this organism, via the tricarboxylic acid cycle (TCA) pathway, under either aerobic-dark or anaerobic-light conditions.

INTERRELATIONSHIPS BETWEEN THE TRICARBOXYLIC ACID AND GLYOXYLATE CYCLES STUDIED WITH BACTERIAL AUXOTROPHS

1962 ◽  
Vol 8 (2) ◽  
pp. 241-247 ◽  
Author(s):  
Henry C. Reeves ◽  
Samuel J. Ajl
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
Tricarboxylic Acid Cycle ◽  
Tricarboxylic Acid ◽  
Mineral Salts ◽  
Glyoxylate Bypass ◽  
Acid Cycle ◽  
E Coli ◽  
Acetate Medium ◽  
Metabolic Significance

An autotroph of Escherichia coli, E26-6, which is unable to grow aerobically in a simple mineral-salts medium with either acetate, glutamate, isocitrate, or any one of the C4 dicarboxylic acid intermediates of the tricarboxylic acid cycle as sole carbon source, has been investigated. The mutant is able to grow, however, in a mineral-salts acetate medium supplemented with any one of the above acids. The specific activities of the tricarboxylic acid cycle and glyoxylate bypass enzymes, with the exception of alpha-ketoglutaric dehydrogenase, which is greatly impaired in the auxotroph, were found to be essentially the same in both the parent and the mutant. Thus, the glyoxylate bypass alone is not capable of supplying sufficient C4 intermediates to allow the growth of E. coli on acetate. Further, there appear to be no other metabolic pathways leading to C4 production, which are of major metabolic significance during growth on acetate, other than the tricarboxylic and glyoxylate cycles. Finally, in conjunction with the tricarboxylic acid cycle, the malate synthetase and isocitritase reactions provide a mechanism which enables E. coli to grow on a medium containing acetate as the sole carbon source.

Saccharomyces cerevisiae contains two functional citrate synthase genes

1986 ◽  
Vol 6 (6) ◽  
pp. 1936-1942
Author(s):  
K S Kim ◽  
M S Rosenkrantz ◽  
L Guarente
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Carbon Source ◽  
Citrate Synthase ◽  
Tricarboxylic Acid Cycle ◽  
Nuclear Gene ◽  
Tricarboxylic Acid ◽  
Yeast Genome ◽  
Gene Encoding ◽  
Acid Cycle ◽  
Nonfermentable Carbon Source

The tricarboxylic acid cycle occurs within the mitochondria of the yeast Saccharomyces cerevisiae. A nuclear gene encoding the tricarboxylic acid cycle enzyme citrate synthase has previously been isolated (M. Suissa, K. Suda, and G. Schatz, EMBO J. 3:1773-1781, 1984) and is referred to here as CIT1. We report here the isolation, by an immunological method, of a second nuclear gene encoding citrate synthase (CIT2). Disruption of both genes in the yeast genome was necessary to produce classical citrate synthase-deficient phenotypes: glutamate auxotrophy and poor growth on rich medium containing lactate, a nonfermentable carbon source. Therefore, the citrate synthase produced from either gene was sufficient for these metabolic roles. Transcription of both genes was maximally repressed in medium containing both glucose and glutamate. However, transcription of CIT1 but not of CIT2 was derepressed in medium containing a nonfermentable carbon source. The significance of the presence of two genes encoding citrate synthase in S. cerevisiae is discussed.

The assimilation of bicarbonate by Neocosmospora vasinfecta

1969 ◽  
Vol 15 (5) ◽  
pp. 389-398 ◽  
Author(s):  
K. Budd
Keyword(s):  
Protein Synthesis ◽  
Carbon Source ◽  
Tricarboxylic Acid Cycle ◽  
Carbon Sources ◽  
Dry Weight ◽  
Insoluble Fraction ◽  
Tricarboxylic Acid ◽  
End Products ◽  
Almost All ◽  
Exogenous Nitrogen

The assimilation of 14C-bicarbonate under controlled conditions was examined in midlog-phase mycelium grown on dextrose as sole carbon source. Sustained assimilation depended on the presence of exogenous nitrogen and carbon sources. When these were provided, assimilation rates of 20–30 μmoles/hour per 100 mg dry weight were maintained for at least 4 hours. After the second hour, almost all of the assimilated bicarbonate-C entered the 80% ethanol-insoluble fraction. Amino acids, especially aspartic and glutamic, were the main destination of assimilated bicarbonate-C; nucleic acids and acids of the tricarboxylic acid cycle accounted for smaller amounts of this carbon. The apparent Km for overall assimilation was 1.4 – 2.2 × 10−4 M with respect to bicarbonate.Assimilation was inhibited by inhibitors of protein synthesis, especially actidione and p-fluorophenylalanine. Evidence was obtained for regulation of assimilation by its end products, and also by the carbon source on which the mycelium was grown. It is concluded that assimilation of bicarbonate or CO2 has an anaplerotic function during protein synthesis in this organism.

scholarly journals Metabolic Engineering of a Novel Propionate-Independent Pathway for the Production of Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) in Recombinant Salmonella enterica Serovar Typhimurium

2002 ◽  
Vol 68 (8) ◽  
pp. 3848-3854 ◽  
Author(s):  
Ilana S. Aldor ◽  
Seon-Won Kim ◽  
Kristala L. Jones Prather ◽  
Jay D. Keasling
Keyword(s):  
Carbon Source ◽  
Salmonella Enterica ◽  
Sole Carbon Source ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Tricarboxylic Acid Cycle ◽  
Expression System ◽  
Tricarboxylic Acid ◽  
Serovar Typhimurium ◽  
Commercial Applications ◽  
Coenzyme B

ABSTRACT A pathway was metabolically engineered to produce poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), a biodegradable thermoplastic with proven commercial applications, from a single, unrelated carbon source. An expression system was developed in which a prpC strain of Salmonella enterica serovar Typhimurium, with a mutation in the ability to metabolize propionyl coenzyme A (propionyl-CoA), served as the host for a plasmid harboring the Acinetobacter polyhydroxyalkanoate synthesis operon (phaBCA) and a second plasmid with the Escherichia coli sbm and ygfG genes under an independent promoter. The sbm and ygfG genes encode a novel (2R)-methylmalonyl-CoA mutase and a (2R)-methylmalonyl-CoA decarboxylase, respectively, which convert succinyl-CoA, derived from the tricarboxylic acid cycle, to propionyl-CoA, an essential precursor of 3-hydroxyvalerate (HV). The S. enterica system accumulated PHBV with significant HV incorporation when the organism was grown aerobically with glycerol as the sole carbon source. It was possible to vary the average HV fraction in the copolymer by adjusting the arabinose or cyanocobalamin (precursor of coenzyme B12) concentration in the medium.

Changes in the oxidative metabolism during maturation of amphibian oocytes

Development ◽  
1980 ◽  
Vol 59 (1) ◽  
pp. 175-186
Author(s):  
Arnaldo H. Legname ◽  
Hortensia Salomón De Legname
Keyword(s):  
Oxidative Metabolism ◽  
Tricarboxylic Acid Cycle ◽  
Mature Oocyte ◽  
Tricarboxylic Acid ◽  
Pentose Phosphate ◽  
Winter Period ◽  
Acid Cycle ◽  
Glycolytic Activity ◽  
Cytoplasmic Maturation ◽  
Respiratory Stimulation

Some aspects of oxidative metabolism during cytoplasmic maturation of the Bufo arenarum oocyte have been studied. During the autumn-winter period (immature oocyte), carbohydrates are degraded through the glycolytic pathway, followed by the classical tricarboxylic acid cycle. During the spring—summer period (mature oocyte), carbohydrates are mainly used through the pentose phosphate cycle, while the tricarboxylic acid cycle operates as the glutamicaspartic cycle. The oxidative phosphorylation of ADP does not seem to change during oocyte maturation. Although maturation does not alter the absolute values of ATP and citrate in the oocyte, it determines their different compartmentalization, which, through phosphofructokinase, in turn regulates the glycolytic activity of the oocyte. Oxygen uptake decreases by about 40 % during maturation, while simultaneously, a marked increment in respiratory stimulation by 2,4-dinitrophenol is observed.

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