Diazotroph establishment and maintenance in the Sorghum – Glomus – Azospirillum association

1989 ◽  
Vol 35 (11) ◽  
pp. 977-981 ◽  
Author(s):  
Raymond S. Pacovsky

Sorghum (Sorghum bicolor (L.) Moench cv. Bok 8) plants were grown in either a high peat or a low organic matter substrate and inoculated with a pigmented strain of Azospirillum brasilense (Cd). Half of the plants were also inoculated with the vesicular–arbuscular mycorrhizal (VAM) fungus Glomus etunicatum, while the remaining plants were fertilized with levels of phosphorus that had been found to be comparable to P input resulting from mycorrhizal colonization. Plants were harvested 1, 3, 5, and 7 weeks postemergence. Total plant dry weight was statistically similar (p > 0.05) between the two treatments for each substrate at all harvests. For each substrate, Azospirillum counts per gram of root were significantly (p < 0.05) higher in mycorrhizal roots at all harvests than in P-fertilized roots, and also higher in the high peat substrate compared with the low organic matter substrate. The ratio of the total number of inoculated A. brasilense cells in the Glomus-colonized compared to the P-fertilized roots was positively and significantly correlated (r = 0.81; p < 0.05) with % VAM colonization. Colonization of sorghum roots by G. etunicatum thus enhanced the establishment and persistence of A. brasilense in the endorhizosphere of sorghum.Key words: Azospirillum brasilense, endomycorrhizae, Glomus etunicatum, rhizosphere, Sorghum bicolor.

2019 ◽  
Vol 7 (2) ◽  
Author(s):  
Nur Edy Suminarti ◽  
A.Y. Edy Guntoro ◽  
A. N. Fajrin

Suminarti et al, 2018. Effect of Source and Dosage of Organic Materials on Changes in Soil Chemical Properties, Growth and Yield of Sorghum Plants (Sorghum bicolor L.Moench) var. KD4 in Dry Land Jatikerto, Malang. JLSO 7(2): Agricultural extensification is the right step to anticipate conditions of food insecurity. This refers to two reasons, namely (1) proliferation of land conversion activities, and (2) sorghum is a carbohydrate-producing plant that is quite tolerant when planted on dry land. The objective of this study was to obtain information about the sources and doses of organic matter that are appropriate to changes in soil chemical properties, growth and yield of sorghum plants, and has been carried out in the dry land of Jatikerto, Malang. A split plot designs with three replications were used in this study, sources of organic material (blothong, UB compost and cow dung) as the main plot, and doses of organic matter (125%, 100% and 75%) as a subplot. Soil analysis was carried out 3 times, i.e. before planting, after application of organic matter and at harvest. The agronomic observations were carried out destructively at 80 days after planting (DAP) including the components of growth (root dry weight, leaf area, and total dry weight of the plant) and harvest at the age of 90 DAP.F test at 5% level was used to test the effect of treatment, while the difference between treatments was based on LSD level of 5%.The results showed that there was a significant interaction between the source and dosage of organic matter on the leaf area and total dry weight, the highest yield was obtained in blothong at various doses. Higher yields of seeds per hectare were also found in blotong: 1.76 tons ha-1, and 1.73 tons ha-1 on 125% doses of organic matter. Blotong application is able to provide elements of N, P and K soil respectively 18.3%, 85.68% and 8.42% for plant.


1993 ◽  
Vol 118 (6) ◽  
pp. 896-901 ◽  
Author(s):  
H. Wang ◽  
S. Parent ◽  
A. Gosselin ◽  
Y. Desjardins

Micropropagated plantlets of Gerbera jamesonii H. Bolus ex Hook. F. `Terra Mix', Nephrolepis exaltata (L.) Schott `Florida Ruffles', and Syngonium podophyllum Schott `White Butterfly' were inoculated with two vesicular-arbuscular mycorrhizal (VAM) fungi, Glomus intraradices Schenck and Smith and G. vesiculiferum Gerderman and Trappe. They were potted in three peat-based media to determine the effects of mycorrhizal peat substrate on acclimatization and subsequent growth of micropropagated plantlets under greenhouse conditions. Symbiosis was established between the three ornamental species and VAM fungi within 4 to 8 weeks of culture in the greenhouse, but not during acclimatization. Mortality of Gerbera and Nephrolepis mycorrhizal plantlets was reduced at week 8 compared to the noninoculated control. A peat-based substrate low in P and with good aeration improved VAM fungi spread and efficiency. Mycorrhizal substrates had a long-term benefit of increasing leaf and root dry weight of Gerbera and Nephrolepis. Mycorrhizal Gerbera plants flowered significantly faster than non-mycorrhizal plants.


2021 ◽  
Vol 10 (2) ◽  
pp. e16710212321
Author(s):  
Larissa Luísa Schumacher ◽  
Julio Viégas ◽  
Tiago João Tonin ◽  
Stela Näetzold Pereira ◽  
Fernando Reimann Skonieski ◽  
...  

A inoculação com Azospirillum brasilense apresenta potencial de uso na cultura do sorgo (Sorghum bicolor [L.] Moench) pela possibilidade de alterar a fisiologia e morfologia das plantas. Neste sentido, o presente estudo tem por objetivo avaliar a influência da inoculação via semente com a bactéria promotora de crescimento da estirpe Azospirillum brasilense sobre a composição morfológica, parâmetros fermentativos, perdas nutricionais, frações nitrogenadas e de carboidratos em silagem de sorgo do híbrido AG 2005. O delineamento experimental foi o inteiramente casualizado, constituindo-se de dois tratamentos (com e sem inoculação), com quatro repetições de silos. O fracionamento nitrogenado, foi determinado conforme descrito por Licitra et al. (1996) e para mensurar o fracionamento dos carboidratos, foi avaliado de acordo com a metodologia proposta por Lanzas et al. (2007). As plantas inoculadas apresentaram menor porcentagem de colmo, mas houve maior participação de panícula mais grãos. O híbrido inoculado produziu silagem com menor teor de nitrogênio insolúvel em detergente ácido (NIDA) e nitrogênio insolúvel em detergente neutro (NIDN). Com relação ao fracionamento de carboidratos, as silagens de sorgo com inoculação diferiram significativamente em CHO (carboidratos totais) e CC (fibra indigestível) em relação ao controle. Sobre o fracionamento proteico, a fração   indigestível (PC), a inoculação apresentou redução de 0,29 unidade percentuais quando comparado ao controle. Desta forma, a inoculação de sementes com A. brasilense pode ser uma alternativa viável, pois apresenta potencial de reduzir a fração de proteína e glicídios insolúvel e indigestível no trato gastrointestinal em silagens de sorgo.


2017 ◽  
Vol 16 (2) ◽  
Author(s):  
Andrian Isro Rahmadi ◽  
Tundjung Tripeni Handayan ◽  
Martha L Lande

The aim of this experiment was to determine the effect of the addition of ash and sand-compost proportion in the media on the growth of sorghum (Sorghum bicolor L.). Theexperiment was conducted on December 2015 to January 2016 in the Botanical Laboratory,Biology Department, Faculty of Mathematic and Natural Science, Lampung University, andwas conducted in a complete randomized design by using two treatments. The first treatmentwas the addition of ash in two equivalents (with and without ash), the second treatment wasthe addition of sand-compost proportion in four equivalents (2 kg of sand, 1¾ kg of sand +¼ kg of compost, 1½ kg of sand + ½ kg of compost, 1¼ kg of sand + ¾ kg of compost). Theobserved variables of the growth were the number of the roots, the crown’s length, the wetweight, and the dry weight. The number of the roots was determined by observing thenumber of the roots present at the base of the stalk of each plants. The length of the crown(cm) was determined by measuring it from the stalk’s base to the edge of the longest leaf.The wet weight (g) was determined by measuring the whole part of the plant using ananalytical balance. The dry weight (g) was measured after the plants were stored in an ovenfor three hours in the temperature of 70o-80oC. The result showed that the best growth wasobtained from the media with the sand equivalent of 1¼ kg of sand added with ¾ kg ofcompost without ash.Key words: Ash, compost, growth of sorghum (Sorghum bicolor L.), sand


1992 ◽  
Vol 1 (5) ◽  
pp. 527-535 ◽  
Author(s):  
Mauritz Vestberg

Ten strawberry cultivars, four early maturing, three late maturing and three “special” cultivars, were inoculated with six strains of vesicular-arbuscular mycorrhizal (VAM) fungi in a pot experiment. Growth effects and colonization of the VAM fungi were studied. Three strains, Glomus macrocarpum V3, G. mosseae Rothamsted and G. sp. V4, were highly efficient, causing significant growth increases in most cultivars. ’Jonsok’ showed the highest mycorrhizal dependency index, 648, and ’Ostara’ the lowest, 269, for the mean response of all six fungi. The fungal strains which increased shoot growth the most also increased the runner plant formation the most. Early cultivars showed higher colonization percentages than late maturing cultivars. Sporulation of the introduced VAM fungi was on average more abundant in early and special cultivars than in late cultivars. Root colonization and strawberry shoot dry weight correlated significantly in most cultivars, but the correlation between colonization and runner formation was generally poor.


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