Chloroplast microsatellites as markers for paternity analysis in Abies alba

This study describes the application of previously characterized chloroplast microsatellites as markers for paternity analysis in a conifer species. The investigations were performed on silver fir (Abies alba Mill.) relic trees in an endangered population of the Ore Mountains (Germany). Two relatively isolated adult trees about 30 m apart, as well as 24 naturally regenerated young trees in their direct neighborhood, were analyzed at two chloroplast microsatellite loci. Results reveal the potential usefulness of the markers for paternity analysis.

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Chloroplast microsatellites as markers for paternity analysis in Abies alba

Canadian Journal of Forest Research ◽

10.1139/cjfr-28-2-317 ◽

1998 ◽

Vol 28 (2) ◽

pp. 317-321 ◽

Cited By ~ 8

Author(s):

B. Ziegenhagen ◽

F. Scholz ◽

A. Madaghiele ◽

G.G. Vendramin

Keyword(s):

Abies Alba ◽

Paternity Analysis ◽

Chloroplast Microsatellites

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Variability of silver fir (Abies alba Mill.) progeny from the Tisovik Reserve expressed in needle traits and chloroplast microsatellite DNA

Biodiversity Research and Conservation ◽

10.1515/biorc-2017-0001 ◽

2017 ◽

Vol 45 (1) ◽

pp. 1-16

Author(s):

Ewa M. Pawlaczyk ◽

Maria A. Bobowicz

Keyword(s):

Genetic Variation ◽

Microsatellite Dna ◽

Abies Alba ◽

Silver Fir ◽

Experimental Plot ◽

Range Limit ◽

Chloroplast Microsatellite ◽

Natural Range ◽

Resin Canals ◽

Białowieża Primeval Forest

AbstractProgeny from nineteen family lines of silver fir (Abies alba Mill.) from the Tisovik Reserve growing in an experimental plot were analyzed based on 4 chloroplast microsatellite DNA loci and 12 morphological and anatomical needle traits. The Tisovik Reserve is located in Białowieża Primeval Forest, 120 km north of the natural range limit of this species, and embraces a small and isolated natural population of silver fir. The aim of this study was to determine genetic variation within and between progeny lines. Analysis of phenotypic variation showed that the traits which differed most among individuals were the needle width and the distance from resin canals to vascular bundle. Those traits, which differed most between the progeny lines, were the number of endodermic cells around the vascular bund and the weight of hypodermic cells. In Tisovik progeny, we detected 107 different haplotypes. In progeny lines, we detected more haplotypes than in maternal trees, and most haplotypes did not exist in maternal trees. This may be the result of pollen influx from other silver fir stands. Progeny from Tisovik showed a higher level of variability in comparison with maternal trees.

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High level of variation at Abies alba chloroplast microsatellite loci in Europe

Molecular Ecology ◽

10.1046/j.1365-294x.1999.00666.x ◽

1999 ◽

Vol 8 (7) ◽

pp. 1117-1126 ◽

Cited By ~ 113

Author(s):

G. G. Vendramin ◽

B. Degen ◽

R. J. Petit ◽

M. Anzidei ◽

A. Madaghiele ◽

...

Keyword(s):

Microsatellite Loci ◽

Abies Alba ◽

Chloroplast Microsatellite ◽

High Level

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Effect of earthworms on mycorrhization, root morphology and biomass of silver fir seedlings inoculated with black summer truffle (Tuber aestivum Vittad.)

Scientific Reports ◽

10.1038/s41598-021-85497-8 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Tina Unuk Nahberger ◽

Gian Maria Niccolò Benucci ◽

Hojka Kraigher ◽

Tine Grebenc

Keyword(s):

Root System ◽

Root Biomass ◽

Fine Root ◽

Abies Alba ◽

Fine Root Biomass ◽

Root Tip ◽

Silver Fir ◽

Tuber Aestivum ◽

Root Tips ◽

Root Parameters

AbstractSpecies of the genus Tuber have gained a lot of attention in recent decades due to their aromatic hypogenous fruitbodies, which can bring high prices on the market. The tendency in truffle production is to infect oak, hazel, beech, etc. in greenhouse conditions. We aimed to show whether silver fir (Abies alba Mill.) can be an appropriate host partner for commercial mycorrhization with truffles, and how earthworms in the inoculation substrate would affect the mycorrhization dynamics. Silver fir seedlings inoculated with Tuber. aestivum were analyzed for root system parameters and mycorrhization, how earthworms affect the bare root system, and if mycorrhization parameters change when earthworms are added to the inoculation substrate. Seedlings were analyzed 6 and 12 months after spore inoculation. Mycorrhization with or without earthworms revealed contrasting effects on fine root biomass and morphology of silver fir seedlings. Only a few of the assessed fine root parameters showed statistically significant response, namely higher fine root biomass and fine root tip density in inoculated seedlings without earthworms 6 months after inoculation, lower fine root tip density when earthworms were added, the specific root tip density increased in inoculated seedlings without earthworms 12 months after inoculation, and general negative effect of earthworm on branching density. Silver fir was confirmed as a suitable host partner for commercial mycorrhization with truffles, with 6% and 35% mycorrhization 6 months after inoculation and between 36% and 55% mycorrhization 12 months after inoculation. The effect of earthworms on mycorrhization of silver fir with Tuber aestivum was positive only after 6 months of mycorrhization, while this effect disappeared and turned insignificantly negative after 12 months due to the secondary effect of grazing on ectomycorrhizal root tips.

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Cross-species microsatellite amplification in Vasconcellea and related genera and their use in germplasm classification

Genome ◽

10.1139/g06-035 ◽

2006 ◽

Vol 49 (7) ◽

pp. 786-798 ◽

Cited By ~ 13

Author(s):

T Kyndt ◽

B Van Droogenbroeck ◽

A Haegeman ◽

I Roldán-Ruiz ◽

G Gheysen

Keyword(s):

Population Genetics ◽

Chloroplast Microsatellites ◽

Microsatellite Data ◽

Chloroplast Microsatellite ◽

Diversity Assessment ◽

Ssr Primers ◽

Polymorphism Rate ◽

Nuclear Ssr ◽

Simple Sequence ◽

Future Population

To generate inexpensive and efficient DNA markers for addressing a number of population genetics problems and identification of wild hybrids in Vasconcellea, we have evaluated the use of simple sequence repeat (SSR) primers previously developed for other species. A set of 103 Vasconcellea accessions and some individuals of the related genera Carica and Jacaratia were analyzed with 10 primer pairs directing amplification of chloroplast microsatellites in Nicotiana tabacum and 9 nuclear SSR primer pairs recently identified in Vasconcellea × heilbornii. Heterologous amplification of chloroplast SSRs was successful for 8 of the 10 loci, of which 6 showed polymorphism. Seven of the 9 nuclear SSR primer pairs were useful in Vasconcellea and often also in Jacaratia and Carica, all revealing polymorphism. Exclusive haplotypes for each described taxon were identified based on chloroplast microsatellite data. Clustering based on separate nuclear and chloroplast data resulted in a clear grouping per taxon, but only low resolution was obtained above species level. The codominancy of nuclear SSRs and the general high polymorphism rate of SSR markers will make them more useful in future population genetics studies and diversity assessment in conservation programs.Key words: Carica, Jacaratia, Vasconcellea, simple sequence repeats, cross-species amplification, classification, interspecific hybrids.

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Impact of Industrial Pollution on Radial Growth of Conifers in a Former Mining Area in the Eastern Carpathians (Northern Romania)

Forests ◽

10.3390/f12050640 ◽

2021 ◽

Vol 12 (5) ◽

pp. 640

Author(s):

Cristian Gheorghe Sidor ◽

Radu Vlad ◽

Ionel Popa ◽

Anca Semeniuc ◽

Ecaterina Apostol ◽

...

Keyword(s):

Air Pollution ◽

Industrial Pollution ◽

Radial Growth ◽

Abies Alba ◽

Mining Operation ◽

Mining Area ◽

Silver Fir ◽

Local Pollution ◽

Eastern Carpathians ◽

The Impact

The research aims to evaluate the impact of local industrial pollution on radial growth in affected Norway spruce (Picea abies (L.) Karst.) and silver fir (Abies alba Mill.) stands in the Tarnița study area in Suceava. For northeastern Romania, the Tarnița mining operation constituted a hotspot of industrial pollution. The primary processing of non-ferrous ores containing heavy metals in the form of complex sulfides was the main cause of pollution in the Tarnița region from 1968 to 1990. Air pollution of Tarnița induced substantial tree growth reduction from 1978 to 1990, causing a decline in tree health and vitality. Growth decline in stands located over 6 km from the pollution source was weaker or absent. Spruce trees were much less affected by the phenomenon of local pollution than fir trees. We analyzed the dynamics of resilience indices and average radial growth indices and found that the period in which the trees suffered the most from local pollution was between 1978 and 1984. Growth recovery of the intensively polluted stand was observed after the 1990s when the environmental condition improved because of a significant reduction in air pollution.

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