In Vivo Bioimaging Using Transgenic Rats and The Role Of Bone Marrow Cells In Wound Healing

2005 ◽  
Vol 09 (14) ◽  
pp. 652-655
Author(s):  
Takashi Murakami ◽  
Hirokazu Inoue ◽  
Eiji Kobayashi
Keyword(s):  
Bone Marrow ◽  
Wound Healing ◽  
Regenerative Medicine ◽  
Bone Marrow Cells ◽  
Transgenic Rats ◽  
Medicine Research ◽  
Bone Marrow Derived Cells ◽  
Marrow Cells

The article discusses the importance of rats in regenerative medicine research. It touches on wound healing using bone marrow derived cells and the artifical dermis.

Improvement of Chronic Wound Healing by Pre-activated Bone Marrow Cells with Sodium Nitroprusside in Rabbits

Drug Research ◽  
2021 ◽  
Author(s):  
Nazira Fatima ◽  
Muhammad Saleem ◽  
Umar Shahbaz
Keyword(s):  
Oxidative Stress ◽  
Bone Marrow ◽  
Wound Healing ◽  
Chronic Wound ◽  
Bone Marrow Cells ◽  
Matrix Deposition ◽  
Cell Based Therapy ◽  
Diabetic Rabbits ◽  
Marrow Cells

Abstract Aim of study This study investigated whether pre-activated bone marrow cells with sodium nitro prusside have effectiveness in the inhibition of diabetic wound healing in diabetic rabbits. In diabetic skin disorders and conditions involved redox state disturbances. The aim was to determine the effect of two minimum dosages of sodium nitro prusside, and its’ potential with bone marrow cells for chronic wound healing in-vivo. Methods Full-thickness skin dorsal wounds were created on diabetic rabbits. The effects of two minimum concentrations of sodium nitro prusside solution with bone marrow cells on wound healing were studied. The useful combination of sodium nitro prusside with bone marrow cells on wound repair may be attributed to its functional influences on inflammation, angiogenesis, cell proliferation, matrix deposition, and remodeling. Results The in-vivo experiments confirmed that pre-activated bone marrow cells contributed to wound healing by alleviating oxidative stress, increasing proliferation and migration, decreasing apoptosis. In histological results, improved collagen deposition, enhanced re-epithelization, angiogenesis, and decreased inflammatory infiltration were also detected in wound biopsies. Conclusions For the treatment of chronic wounds, cell-based therapy was an attractive approach. Bone marrow cells have a low ability to differentiate various types of cells or late healing without pretreatment. So it was needed to increase their potency of differentiation. The transplantation of pretreated bone marrow cells with a prime quantity of sodium nitro prusside solution improved chronic wound healing with a greater level of growth factors and a minimum level of oxidative stress.

DEFICIENCY IN PROTEINASE-ACTIVATED RECEPTOR-2 (PAR2) ATTENUATES COLONIC INFLAMMATION IN MICE INDEPENDENT OF BONE MARROW DERIVEDCELLS.

2008 ◽  
Vol 31 (4) ◽  
pp. 13
Author(s):  
Eric Hyun ◽  
Patricia Andrade-Gordon ◽  
Nathalie Vergnolle
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Similar Degree ◽  
Colonic Inflammation ◽  
Inflammatory Parameters ◽  
Donor Bone Marrow ◽  
Bowel Diseases ◽  
Bone Marrow Derived Cells ◽  
Marrow Cells

Background andaims: The role of PAR_2 duringintestinal inflammation is unclear, since its activation in the gut could lead to either pro- or anti-inflammatory properties. The aim of this study was to investigate the effects of PAR_2 deficiency (using PAR_2-deficient mice: PAR_2 ^-/-)in an animal model of colitis and to investigate the role of PAR_2 deficiency on bone marrow-derived cells. Methods: Colonic inflammation in PAR_2 ^+/+ and PAR_2 ^-/- mice was induced by 2.5 % dextran sodium sulfate(DSS). Chimeric mice (PAR_2^Ch+/+ or PAR_2^Ch-/-) injected with bone marrow cells (BMC) from either PAR_2 ^+/+ or PAR_2 ^-/- mice, were also given DSS. Results: PAR_2 ^+/+ mice treated with DSS showed a significant increase in leukocyte rolling/adherence, bowel thickness, myeloperoxidase (MPO) activity and macroscopic damage compared to PAR_2 ^-/-. PAR_2^Ch-/- mice, regardless of the source of bone marrow cells injected, showed significantly reduced inflammatory parameters, compared to PAR_2^Ch+/+ injected with PAR_2 ^+/+ bone marrow. A similar degree of DSS-induced inflammation was observed between chimeric PAR_2^Ch+/+ mice injected with either PAR_2 ^-/- or PAR_2 ^+/+bone marrow cells. Conclusions: Since DSS inflammation was reduced in PAR_2^ch-/- compared with PAR_2^ch+/+ mice, irrespective of the source of donor bone marrow cells, we conclude that PAR_2 expression on recipient tissues rather than on donor bone marrow cells plays a key role in the development and maintenance of DSS-induced colitis. PAR_2 thus appears as an important mediator of colonic inflammation and represents apotential target for the treatment of inflammatory bowel diseases. Supported by CIHR and the Crohn's and Colitis Foundation of Canada

scholarly journals Role of IL-4 in bone marrow driven dysregulated angiogenesis and age-related macular degeneration

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Takashi Baba ◽  
Dai Miyazaki ◽  
Kodai Inata ◽  
Ryu Uotani ◽  
Hitomi Miyake ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Macular Degeneration ◽  
Bone Marrow Cells ◽  
Tube Formation ◽  
Age Related Macular Degeneration ◽  
Sterile Inflammation ◽  
Age Related ◽  
Marrow Cells

Age-associated sterile inflammation can cause dysregulated choroidal neovascularization (CNV) as age-related macular degeneration (AMD). Intraocular fluid screening of 234 AMD patients identified high levels of IL-4. The purpose of this study was to determine the functional role of IL-4 in CNV formation using murine CNV model. Our results indicate that the IL-4/IL-4 receptors (IL4Rs) controlled tube formation and global proangiogenic responses of bone marrow cells. CCR2+ bone marrow cells were recruited to form very early CNV lesions. IL-4 rapidly induces CCL2, which enhances recruitment of CCR2+ bone marrow cells. This in vivo communication, like quorum-sensing, was followed by the induction of IL-4 by the bone marrow cells during the formation of mature CNVs. For CNV development, IL-4 in bone marrow cells are critically required, and IL-4 directly promotes CNV formation mainly by IL-4R. The IL-4/IL-4Rα axis contributes to pathological angiogenesis through communications with bone marrow cells leading to retinal degeneration.

Role of clozapine in the occurrence of chromosomal abnormalities in human bone-marrow cells in vivo and in cultured lymphocytes in vitro

1977 ◽  
Vol 38 (1) ◽  
pp. 77-89 ◽  
Author(s):  
Sakari Knuutila ◽  
Eila Helminen ◽  
Leena Knuutila ◽  
Seppo Leisti ◽  
Martti Siimes ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Chromosomal Abnormalities ◽  
Bone Marrow Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Marrow Cells

scholarly journals CD34+ human marrow cells that express low levels of Kit protein are enriched for long-term marrow-engrafting cells

Blood ◽  
1996 ◽  
Vol 87 (10) ◽  
pp. 4136-4142 ◽  
Author(s):  
I Kawashima ◽  
ED Zanjani ◽  
G Almaida-Porada ◽  
AW Flake ◽  
H Zeng ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Marrow Cell ◽  
In Utero ◽  
Fetal Sheep ◽  
Hematopoietic Stem ◽  
Show Evidence ◽  
Marrow Cells

Using in utero transplantation into fetal sheep, we examined the capability of human bone marrow CD34+ cells fractionated based on Kit protein expression to provide long-term in vivo engraftment. Twelve hundred to 5,000 CD34+ Kit-, CD34+ Kit(low), and CD34+ Kit(high) cells were injected into a total of 14 preimmune fetal sheep recipients using the amniotic bubble technique. Six fetuses were killed in utero 1.5 months after bone marrow cell transplantation. Two fetuses receiving CD34+ Kit(low) cells showed signs of engraftment according to analysis of CD45+ cells in their bone marrow cells and karyotype studies of the colonies grown in methylcellulose culture. In contrast, two fetuses receiving CD34+ Kit(high) cells and two fetuses receiving CD34+ Kit- cells failed to show evidence of significant engraftment. Two fetuses were absorbed. A total of six fetuses receiving different cell populations were allowed to proceed to term, and the newborn sheep were serially examined for the presence of chimerism. Again, only the two sheep receiving CD34+ Kit(low) cells exhibited signs of engraftment upon serial examination. Earlier in studies of murine hematopoiesis, we have shown stage-specific changes in Kit expression by the progenitors. The studies of human cells reported here are in agreement with observations in mice, and indicate that human hematopoietic stem cells are enriched in the Kit(low) population.

Distribution of sister chromatid exchanges on the mouse chromosomes in vivo with reference to the replication properties of the X chromosome

1984 ◽  
Vol 26 (2) ◽  
pp. 152-157
Author(s):  
S. M. Singh ◽  
D. L. Reimer
Keyword(s):  
Bone Marrow ◽  
X Chromosome ◽  
Sister Chromatid ◽  
Bone Marrow Cells ◽  
Relative Length ◽  
Chromosome Length ◽  
Sister Chromatid Exchanges ◽  
Chromatid Exchanges ◽  
Marrow Cells

Frequency of sister chromatid exchanges (SCE) were recorded separately for different chromosomes from bone marrow cells of female mice of the two genetic strains (C3H/S and C57BL/6J). SCEs were evaluated following different doses of 5-bromo-2′deoxyuridine (BrdU) as nine hourly i.p. injections. The SCE per cell increased with increasing BrdU doses which was slightly higher in C3H/S than in the C57BL/6J. SCEs per cell were variable at every treatment – strain combination, possibly reflecting the heterogeneous nature of the bone marrow cells. In general, there is a positive correlation between SCE per chromosome and the relative chromosome length. Total SCEs on one of the large chromosomes (most likely the X chromosome), however, are significantly higher than expected on the basis of relative length alone. Most of this increase is attributable to one of the homologues of this chromosome, which is not in synchrony with the rest of the chromosomes and may represent the late-replicating X. These results when viewed in the light of replication properties of the heterochromatinized X, suggest a direct involvement of DNA replication in SCE formation and may argue against the replication point as the sole site for the SCEs.Key words: sister chromatid exchange, BrdU, recombination, replication, X chromosome.

Transplantation of Bone Marrow Cells Preactivated With Sodium Nitroprusside Improves Acute Wound Healing in Rabbits

2021 ◽  
pp. 153473462110290
Author(s):  
Nazira Fatima ◽  
Muhammad Saleem
Keyword(s):  
Bone Marrow ◽  
Wound Healing ◽  
Sodium Nitroprusside ◽  
Bone Marrow Cells ◽  
Healing Process ◽  
Wound Healing Process ◽  
Inflammatory Infiltration ◽  
Acute Wound ◽  
High Concentrations ◽  
Marrow Cells

The development of wound healing impairment mainly represents challenging clinical problems. The less and high concentrations of nitric oxide can influence angiogenesis, remodeling, and proliferation of skin cells. Delayed acute wounds generally have failed to progress via the normal stages of healing. Such wounds usually enter a state of pathological inflammation due to a postponed, incomplete, and uncoordinated healing process. This study aimed to investigate the effect of normal bone marrow cells (BMCs) and preconditioning of BMCs with minimum concentrations of sodium nitroprusside (NaNP) solution for acute wound healing. For acute wound healing, full-thickness dorsal wounds were created on rabbits. The acute wound of rabbits was treated with BMCs and preactivated BMCs with NaNP. Histological results showed that BMCs preactivated with NaNP could improve collagen deposition, enhanced reepithelization, and decreased inflammatory infiltration. Overall, BMCs treated with NaNP can help to improve acute wound healing in rabbits. The result strongly confirmed the beneficial effect in augmenting the wound healing process. The combination of BMCs with NaNP was safe and convenient for acute wound healing.

scholarly journals AML cells are differentially sensitive to chemotherapy treatment in a human xenograft model

Blood ◽  
2013 ◽  
Vol 121 (12) ◽  
pp. e90-e97 ◽  
Author(s):  
Mark Wunderlich ◽  
Benjamin Mizukawa ◽  
Fu-Sheng Chou ◽  
Christina Sexton ◽  
Mahesh Shrestha ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Induction Therapy ◽  
Bone Marrow Cells ◽  
Xenograft Model ◽  
Chemotherapy Treatment ◽  
Key Points ◽  
Increased Sensitivity ◽  
Total Bone Marrow ◽  
Marrow Cells

Key Points A relevant xenograft chemotherapy model was developed by using standard AML induction therapy drugs and primary human AML patient samples. Human AML cells show significantly increased sensitivity to in vivo chemotherapy treatment compared with murine LSK and total bone marrow cells.

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