Construction of Nano-Carriers Coated with Platelet Membrane and Its Application in Targeted Therapy of Inflammation

In this study, poly (lactic acid-glycolic acid) (PLGA) nanoparticles loaded with anti-inflammatory drug ketoprofen (KET) were prepared and then coated with platelet membrane (PLTM) to form KET@PLTM-PLGA nano-particles (NPs). The particle size of the KET@PLTM-PLGA NPs is 176[Formula: see text]nm and the surface protein is the same as that of PLTM. The results of confocal microscopy and flow cytometry showed that the KET@PLTM-PLGA NPs uptake of RAW264.7 induced by LPS was significantly higher than that of KET@PLGA NPs, without PLTM, which was due to the binding of P-selectin to CD44 receptors on the surface of RAW264.7 cells induced by LPS on the surface of PLTM. Compared with other KET preparations, KET@PLTM-PLGA NPs have better anti-inflammatory effect.

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A Novel Sustained Anti-Inflammatory Effect of Atorvastatin—Calcium PLGA Nanoparticles: In Vitro Optimization and In Vivo Evaluation

Pharmaceutics ◽

10.3390/pharmaceutics13101658 ◽

2021 ◽

Vol 13 (10) ◽

pp. 1658

Author(s):

Dalia H. Abdelkader ◽

Ahmed Kh. Abosalha ◽

Mohamed A. Khattab ◽

Basmah N. Aldosari ◽

Alanood S. Almurshedi

Keyword(s):

Particle Size ◽

Zeta Potential ◽

In Vitro Release ◽

In Vivo Evaluation ◽

Water Emulsion ◽

Atorvastatin Calcium ◽

Anti Inflammatory ◽

Inflammatory Effect

Atorvastatin Calcium (At-Ca) has pleiotropic effect as anti-inflammatory drug beside its main antihyperlipidemic action. Our study was conducted to modulate the anti-inflammatory effect of At-Ca to be efficiently sustained for longer time. Single oil-water emulsion solvent evaporation technique was used to fabricate At-Ca into polymeric nanoparticles (NPs). In vitro optimization survey was performed on Poly(lactide-co-glycolide) (PLGA) loaded with At-Ca regrading to particle size, polydispersity index (PDI), zeta potential, percent entrapment efficiency (% EE), surface morphology and in vitro release pattern. In vitro drug-polymers interactions were fully scanned using Fourier-Transform Infrared Spectroscopy (FTIR) and Differential Scanning calorimetry (DSC) proving that the method of fabrication is an optimal strategy maintaining the drug structure with no interaction with polymeric matrix. The optimized formula with particle size (248.2 ± 15.13 nm), PDI (0.126 ± 0.048), zeta potential (−12.41 ± 4.80 mV), % EE (87.63 ± 3.21%), initial burst (39.78 ± 6.74%) and percent cumulative release (83.63 ± 3.71%) was orally administered in Male Sprague–Dawley rats to study the sustained anti-inflammatory effect of At-Ca PLGA NPs after carrageenan induced inflammation. In vivo results demonstrate that AT-Ca NPs has a sustained effect extending for approximately three days. Additionally, the histological examination revealed that the epidermal/dermal layers restore their typical normal cellular alignment with healthy architecture.

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Anti-Inflammatory Evaluation of NLC (Nanostructured Lipid Carriers) Meloxicam In-Vivo

International Journal of Drug Delivery Technology ◽

10.25258/ijddt.v7i04.10657 ◽

2017 ◽

Vol 7 (04) ◽

Author(s):

Widyaningrum I. ◽

Hariyadi D. M. ◽

Hendradi E.

Keyword(s):

Particle Size ◽

Research Result ◽

Entrapment Efficiency ◽

Solid Lipid ◽

Rat Paw Edema ◽

Auc Value ◽

Anti Inflammatory ◽

Rat Paw ◽

Inflammatory Effect

Objective: The aim of this research study was to investigate the anti-inflammatory effect of NLC meloxicam. NLC contains solid and liquid lipid. Monostearin as solid lipid and Miglyol 808 as liquid lipid. Methods : NLC meloxicam was repared using emulsification methodwith three different lipid ratio. . NLC meloxicam was prepared and characterized for measuring the pH, viscocity, particle size, and entrapment efficiency. The rat paw edema test was performed to evaluate the antiinflammatory activity of three formulations NLC meoxicam. Results : based on research result shows that the smaller the solid lipid concentrations, particle size is the larger, the greater viscosity, thus increasing occlusive NLC to the skin. The third formula hasthe greatest solid lipid concentration shows the smallest AUC value but once in a statistical test known to be significantly different from the three formulas. Conclusions : NLC meloxicam showed that it had anti-inflammatory effectiveness

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AB0175 INNOVATIVE PREPARATION OF CURCUMIN NANOPARTICLES TO IMPROVE ANTI-INFLAMMATORY EFFECT IN RHEUMATIC DISEASE

Annals of the Rheumatic Diseases ◽

10.1136/annrheumdis-2020-eular.5157 ◽

2020 ◽

Vol 79 (Suppl 1) ◽

pp. 1387.1-1387

Author(s):

A. Soltani ◽

N. Abdolahi ◽

Y. Ghanbari

Keyword(s):

Particle Size ◽

Rheumatic Disease ◽

Polyvinyl Alcohol ◽

Cell Line ◽

Cytotoxic Effect ◽

Colloidal Suspension ◽

Great Promise ◽

Curcumin Nanoparticles ◽

Anti Inflammatory ◽

Inflammatory Effect

Background:Curcumin (Cur) as a natural compound can be used in the wide spectrum of healthy functions and pharmacological activities [1-4]. It shows great promise for medication of various pro-inflammatory chronic illnesses [5]. In this study, we evaluate the ability of poly(lactide-co-glycolide)(PLGA) and different grads of PVA (polyvinyl alcohol) and lecithin as a drug delivery system for poorly soluble CurObjectives:The goal of this study was to prepare and characterize Cur encapsulated PLGA and different grads of PVA and lecithin as an efficient nanocarrier for improve anti-inflammatory effect in rheumatic diseaseMethods:The PLGA nanospheres were formulated and then characterized for percent yield, encapsulation efficiency, surface morphology, and in vitro drug release profiles. At first, 6 mg of Cur was added to the organic phase including 24 mg of polymer dissolved in 5 mL of dichloromethane to constitute 1:4 (drug-to-polymer) ratios. Then, a mixture of PVA-lecithin (at about 5 cc) was added to maintain the stability of double emulsion droplets. The emulsion was continuously stirred at 300 rpm for 24 hours (at temperature of 37.5 ˚C) to evaporate the solvent, leaving behind the colloidal suspension of the drug-encapsulated nanoparticle in aqueous phase. The encapsulation of Cur into PLGA was characterized by Fourier transform infrared spectroscopy (FT-IR) and Transmission electron microscopy (TEM).Results:Our studies achieved the successful formation of smooth surface and spherical shape Cur encapsulated into PLGA nanoparticles by the TEM image confirmed. The particle size distribution demonstrated a range of 30 nm to 100 nm, with the mean particle size being 45 nm. FTIR study implies successful loading of Cur into the nanoparticles. We show high drug-loading efficiency about 98 ± 0.5% for 6% of Cur weight in total ingredients weight of PLGA (w/w). It was also seen that a slower sustained release of 10% CUR in 48 hours is observed with biocompatible PLGA in phosphate buffered saline (pH = 7.4). The MTT assay of the Cur-PLGA exhibited no cytotoxic effect on Normal mouse fibroblast cells (L-929) cell line. IC50 of Cur -PLGA increased 99.5% against Cur nanoparticles (33.57 ±0.62 µM) (P < 0.05).Conclusion:In this study, we constructed a novel preparation of curcumin nanoparticles with PLGA and different grads of PVA (polyvinyl alcohol) and lecithin to improve the bioavailability of CUR and PLGA exhibited no cytotoxic effect on L-929 cell lineReferences:In this study, we constructed a novel preparation of curcumin nanoparticles with PLGA and different grads of PVA (polyvinyl alcohol) and lecithin to improve the bioavailability of CUR and PLGA exhibited no cytotoxic effect on L-929 cell lineDisclosure of Interests:None declared

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Preparation of Poly[lactic-co-glycolic] Acid Nanospheres and Its Role in Hepatoma Cells

Journal of Nanoscience and Nanotechnology ◽

10.1166/jnn.2021.18627 ◽

2021 ◽

Vol 21 (2) ◽

pp. 977-986

Author(s):

Zhongxing Shi ◽

Jinping Li ◽

Hongwei Liang ◽

Hongbo Hu ◽

Huijie Jiang

Keyword(s):

Particle Size ◽

Release Rate ◽

Hepg2 Cells ◽

Glycolic Acid ◽

Drug Loading ◽

Average Particle Size ◽

Plga Nanoparticles ◽

Toxin Gene ◽

Average Particle

Poly[lactic-co-glycolic] acid (PLGA) targeting nanoparticles AFP/PLGA/Dt386, loaded with Dt386 plasmid of diphtheria toxin gene, modified by Alpha fetoprotein (AFP) monoclonal antibody, is prepared. Its physical and chemical properties and its effect on HepG2 cells are studied. Firstly, Dt386 expression plasmid pET11a/Dt386 is constructed and PLGA nanoparticles are prepared by emulsion solvent evaporation (ESE). Scanning electron microscope (SEM) is used to observe its morphology. Laser Particle Sizer is used to measure the particle size. In addition, the encapsulation efficiency, drug loading and in vitro release rate of PLGA nanoparticles are measured. Carboxy fluorescein and rhodamine fluorescein are used to double label IgG/PLGA/Dt386 and AFP/PLGA/Dt386 nanospheres, respectively, the entry of nanospheres into HepG2 cells are observed at 3 h and 12 h. The effect of AFP/PLGA/Dt386 nanospheres on the migration of HepG2 cells is examined by wounding healing assay. Transwell chamber experiment is used to detect the effect of AFP/PLGA/Dt386 nanospheres on the invasion of HepG2 cells. MTT method is utilized to determine the inhibitory activity of nanoparticles on HepG2 cell proliferation. After treated with IgG/PLGA/Dt386 and AFP/PLGA/Dt386 nanoparticles for 48 hours, flow cytometry is used to detect the apoptosis rate and cell cycle of HepG2 cells in each group. The results show that the prepared nanospheres have regular morphology, flat surface, average particle size of 265.72±12.46 nm, zeta potential of −18.15 mV. The average entrapment efficiency and drug loading are 78.48±1.71% and 3.16±0.35%, respectively. The nanoparticles release slowly and stably in vitro. At the 10th day, the release rate reaches 75.13%. PLGA nanospheres can effectively protect DNA from nuclease degradation. The results show that AFP/PLGA/Dt386 nanospheres have biological targeting effect and can be enriched in cells. AFP/PLGA/Dt386 nanoparticles can significantly inhibit the migration, invasion and proliferation of HepG2 cells, and promote apoptosis.

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Protective and anti-inflammatory effect of selenium nano-particles against bleomycin-induced pulmonary injury in male rats

Drug and Chemical Toxicology ◽

10.1080/01480545.2018.1560466 ◽

2019 ◽

Vol 44 (1) ◽

pp. 92-100 ◽

Cited By ~ 2

Author(s):

Rana Shahabi ◽

Ali Anissian ◽

Seyed Ali Javadmoosavi ◽

Farinaz Nasirinezhad

Keyword(s):

Nano Particles ◽

Male Rats ◽

Pulmonary Injury ◽

Anti Inflammatory ◽

Inflammatory Effect

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Particle size determines the anti-inflammatory effect of wheat bran in a model of fructose over-consumption: Implication of the gut microbiota

Journal of Functional Foods ◽

10.1016/j.jff.2017.12.035 ◽

2018 ◽

Vol 41 ◽

pp. 155-162 ◽

Cited By ~ 14

Author(s):

Francesco Suriano ◽

Audrey M. Neyrinck ◽

Joran Verspreet ◽

Marta Olivares ◽

Sophie Leclercq ◽

...

Keyword(s):

Particle Size ◽

Gut Microbiota ◽

Wheat Bran ◽

Anti Inflammatory ◽

Inflammatory Effect

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Composite scaffold of micronized porcine cartilage/poly(lactic‑co‑glycolic acid) enhances anti-inflammatory effect

Materials Science and Engineering C ◽

10.1016/j.msec.2018.02.020 ◽

2018 ◽

Vol 88 ◽

pp. 46-52 ◽

Cited By ~ 5

Author(s):

Soomin Kim ◽

Ji Eun Jang ◽

Ju Hee Lee ◽

Gilson Khang

Keyword(s):

Glycolic Acid ◽

Composite Scaffold ◽

Anti Inflammatory ◽

Inflammatory Effect

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Reduction of paw edema and liver oxidative stress in carrageenan-induced acute inflammation by Lobaria pulmonaria and Parmelia caperata, lichen species, in mice

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000620 ◽

2019 ◽

pp. 1-9

Author(s):

Samira Salem ◽

Essaid Leghouchi ◽

Rachid Soulimani ◽

Jaouad Bouayed

Keyword(s):

Time Course ◽

Acute Inflammation ◽

Lichen Species ◽

Paw Edema ◽

Sod Activity ◽

Edema Formation ◽

Lobaria Pulmonaria ◽

Anti Inflammatory ◽

Endogenous Antioxidant ◽

Inflammatory Effect

Abstract. Paw edema volume reduction is a useful marker in determining the anti-inflammatory effect of drugs and plant extracts in carrageenan-induced acute inflammation. In this study, the anti-inflammatory effect of Lobaria pulmonaria (LP) and Parmelia caperata (PC), two lichen species, was examined in carrageenan-induced mouse paw edema test. Compared to the controls in carrageenan-induced inflammation (n = 5/group), our results showed that pretreatment by single oral doses with PC extract (50–500 mg/kg) gives better results than LP extract (50–500 mg/kg) in terms of anti-edematous activity, as after 4 h of carrageenan subplantar injection, paw edema formation was inhibited at 82–99% by PC while at 35–49% by LP. The higher anti-inflammatory effect of PC, at all doses, was also observed on the time-course of carrageenan-induced paw edema, displaying profile closely similar to that obtained with diclofenac (25 mg/kg), an anti-inflammatory drug reference (all p < 0.001). Both LP and PC, at all doses, significantly ameliorated liver catalase (CAT) activity (all p < 0.05). However, superoxide dismutase (SOD) activity, glutathione peroxidase (GPx) activity and glutathione (GSH) levels were found increased in liver of PC- compared to LP-carrageenan-injected mice. Our findings demonstrated on one hand higher preventive effects of PC compared to LP in a mouse carrageenan-induced inflammatory model and suggested, on the other hand, that anti-inflammatory effects elicited by the two lichens were closely associated with the amelioration in the endogenous antioxidant status of liver.

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