scholarly journals Polarimetry feature parameter deriving from Mueller matrix imaging and auto-diagnostic significance to distinguish HSIL and CSCC

2021 ◽  
Author(s):  
Anli Hou ◽  
Xingjian Wang ◽  
Yujuan Fan ◽  
Wenbin Miao ◽  
Yang Dong ◽  
...  
Keyword(s):  
Squamous Epithelium ◽  
Imaging Techniques ◽  
Mueller Matrix ◽  
Pathological Diagnosis ◽  
Pathological Examination ◽  
Epithelial Tissue ◽  
Cervical Lesions ◽  
Diagnostic Significance ◽  
Polarization Imaging ◽  
Linear Discriminant

High-grade squamous intraepithelial lesion (HSIL) is regarded as a serious precancerous state of cervix, and it is easy to progress into cervical invasive carcinoma which highlights the importance of earlier diagnosis and treatment of cervical lesions. Pathologists examine the biopsied cervical epithelial tissue through a microscope. The pathological examination will take a long time and sometimes results in high inter- and intra-observer variability in outcomes. Polarization imaging techniques have broad application prospects for biomedical diagnosis such as breast, liver, colon, thyroid and so on. In our team, we have derived polarimetry feature parameters (PFPs) to characterize microstructural features in histological sections of breast tissues, and the accuracy for PFPs ranges from 0.82 to 0.91. Therefore, the aim of this paper is to distinguish automatically microstructural features between HSIL and cervical squamous cell carcinoma (CSCC) by means of polarization imaging techniques, and try to provide quantitative reference index for pathological diagnosis which can alleviate the workload of pathologists. Polarization images of the H&E stained histological slices were obtained by Mueller matrix microscope. The typical pathological structure area was labeled by two experienced pathologists. Calculate the polarimetry basis parameter (PBP) statistics for this region. The PBP statistics (stat_PBPs) are screened by mutual information (MI) method. The training method is based on a linear discriminant analysis (LDA) classifier which finds the most simplified linear combination from these stat_PBPs and the accuracy remains constant to characterize the specific microstructural feature quantitatively in cervical squamous epithelium. We present results from 37 clinical patients with analysis regions of cervical squamous epithelium. The accuracy of PFP for recognizing HSIL and CSCC was 83.8% and 87.5%, respectively. This work demonstrates the ability of PFP to quantitatively characterize the cervical squamous epithelial lesions in the H&E pathological sections. Significance: Polarization detection technology provides an efficient method for digital pathological diagnosis and points out a new way for automatic screening of pathological sections.

scholarly journals Prevalence of human papillomavirus genotypes and precancerous cervical lesions in a screening population in Beijing, China: Analysis of results from China’s Top 3 Hospital, 2009–2019

2020 ◽  
Author(s):  
Yidi Liu ◽  
Huan Wu ◽  
Jingjiang Xu ◽  
Defu Chen ◽  
Hongyou Zhao ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Intraepithelial Neoplasia ◽  
Northern China ◽  
Pathological Diagnosis ◽  
Pathological Examination ◽  
Low Grade ◽  
Cervical Lesions ◽  
Hpv Genotyping ◽  
Squamous Intraepithelial Neoplasia ◽  
Hpv Genotypes

Abstract Background: Cervical cancer is the fourth most common cancer in women. Early detection and diagnosis play an important role in secondary prevention of cervical cancer. This study aims to provide more information to develop an effective strategy for the prevention and control of cervical cancer in northern China. Methods: A retrospective single-centre descriptive cross-sectional study was conducted in Chinese PLA General Hospital located in Beijing, covering the period from January 2009 to June 2019. The patients who underwent a polymerase chain reaction (PCR)-based HPV genotyping test and cervical pathological diagnosis were included. Furthermore, we limited the interval between the two examination within 180 days for the purpose of making sure their correlation to analyse their relationship. Moreover, the relationship between different cervical lesions and age as well as single/multiple HPV infection was assessed.Results: A total of 3,134 patients were eligible in this study after HPV genotyping test and pathological diagnosis. Most of the patients (95%) were from northern China. Among the patients, 1,745(55.68%） had high-grade squamous intraepithelial neoplasia (HSIL), 1,354 (43.20%) had low-grade squamous intraepithelial neoplasia (LSIL) and 35 (1.12%) were Normal. The mean age was 42.06 ± 10.82(range, 17–79 years). The women aged 35-49 years accounted for the highest incidence rate. The top five most commonly identified HPV genotypes in each lesion class were as follows: HPV16, 58, 52, 31 and 51 in the class of HSIL; HPV16, 52, 58, 56 and 51 in the class of LSIL; HPV16, 31, 6,11, 52 and 58 in the class of normal. The frequencies of HPV single genotype infection and multiple genotypes infection were 55.26% and 34.18%, respectively. There was no difference in the attributable proportions of multiple genotypes infection amongst HSIL, LSIL and Normal.Conclusions: In Northern China, HPV16 was the most dominant genotype in the patients with pathological examination. The peak age of the onset of HSIL was between 35 and 49 years of age. Infection with multiple HPV genotypes did not increase the risk of HSIL. Type-specific HPV prevalence and attribution proportion to cervical precancerous lesions should be taken into consideration in the development of vaccines and strategy for screening in this population.

scholarly journals Mesenteric Cysts: A Retrospective Review. Its Not All That Simple

2020 ◽  
Vol 154 (Supplement_1) ◽  
pp. S117-S118
Author(s):  
M Bourgeau ◽  
V Avadhani
Keyword(s):  
Cyst Wall ◽  
Imaging Techniques ◽  
Case Reports ◽  
Lymphatic Vessels ◽  
Mucinous Cystadenoma ◽  
Final Diagnosis ◽  
Cystic Mass ◽  
Pathological Examination ◽  
Urachal Cyst ◽  
Mesenteric Cysts

Abstract Introduction/Objective Mesenteric cysts are rare intra-abdominal lesions in adults. However, with the advanced imaging techniques and laparoscopic techniques, they are more often being identified and resected when clinically significant. There is a lack of detailed information in histopathology (except as case reports) since mesentery is generally neglected in our organ-based textbooks. The aim of our study is to highlight the importance of identifying and classifying mesenteric cystic lesions; they are not all that simple. Methods We performed a retrospective search on all mesenteric cysts submitted as excisions in our electronic database from 2013-2019. We classified them as per the de Perrot (PMID: 11053936) classification with modification. Results Our search showed: A. Lymphatic origin-11 (lymphangioma-10, Lymphangioma hamartomatous-1, associated with LAM-0), B. Mesothelial origin-68 (Benign mesothelial cysts-57, multilocular mesothelial cyst-11), C. Enteric origin- 3, D. Urogenital origin (Urachal cyst, mullerian inclusion cyst)-9, E. Mature cystic teratom-2, F. Pseudocyst-12, G. Epithelial cyst (not urogenital)- 11 (a/w LAMN-3, MCN-4, Mucinous cystadenoma-4), H. Associated with carcinoma-2. Case illustration: A 61-year-old male presented with worsening dysphagia, emesis and hiccups. A CT scan showed a 21.2 cm cystic mass with at least one septation (Fig 1). The cyst was resected. On gross pathological examination, the cyst measured 18 cm in greatest dimension with a thick, rough, tan-brown capsule. Microscopic examination showed a fibrous capsule, and cyst wall composed of numerous lymphatic vessels (CD31 positive) and prominent smooth muscle proliferation (Desmin positive). Scattered lymphoid aggregates were also present throughout the cyst wall. No definite epithelial lining was identified and was suspected to have been denuded. HMB-45 immunostain was negative, ruling out association with LAM. The final diagnosis of a Lymphangiomyoma, hamartomatous was rendered. Conclusion Though most of the mesenteric cysts are benign, some of them are significantly important such as Lymphangiomyoma (esp secondary to LAM), MCN, those associated with LAMN etc. and identifying and differentiating from their mimics has distinct clinical implications.

scholarly journals Oesophageal squamous cell carcinoma mimicking submucosal tumour

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Wen Wang ◽  
Dazhou Li ◽  
Linfu Zheng ◽  
Hongli Zhan
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Squamous Epithelium ◽  
Pathological Examination ◽  
Muscularis Mucosa ◽  
Case Presentation ◽  
Duct Epithelium ◽  
Tumour Spread ◽  
Positron Emission

Abstract Background Oesophageal submucosal tumours are usually benign. We report a rare case of esophageal squamous cell carcinoma presenting as a submucosal tumour. Case presentation A 58-year-old man undergoing screening oesophago-gastroduodenoscopy was found to have a smooth-surfaced 0.6-cm sized submucosal tumour in the oesophagus 30 cm from the incisor. Endoscopic ultrasonography showed the tumour to be located in the muscularis mucosa; the lesion was heterogeneously hypoechoic and had a clear boundary. With a provisional diagnosis of leiomyoma, the tumour was removed by endoscopic submucosal dissection. Pathological examination showed it to be a moderately differentiated infiltrating squamous cell carcinoma, with normal overlying squamous epithelium. Immunohistochemistry indicated that it was caused by malignant transformation in mucosal glandular duct epithelium. Positron emission tomography–computer tomography showed no tumour spread to any other site. The patient was treated by oesophageal resection. Conclusion The clinician should be aware that oesophageal submucosal tumours with smooth overlying mucosa may not always be benign; malignancy must be ruled out.

scholarly journals Real-time intraoperative glioma diagnosis using fluorescence imaging and deep convolutional neural networks

2021 ◽  
Author(s):  
Biluo Shen ◽  
Zhe Zhang ◽  
Xiaojing Shi ◽  
Caiguang Cao ◽  
Zeyu Zhang ◽  
...  
Keyword(s):  
Neural Networks ◽  
Real Time ◽  
Fluorescence Imaging ◽  
Convolutional Neural Networks ◽  
Frozen Section ◽  
Fluorescent Imaging ◽  
Pathological Diagnosis ◽  
Pathological Examination ◽  
Deep Convolutional Neural Networks ◽  
Fluorescence Images

Abstract Purpose Surgery is the predominant treatment modality of human glioma but suffers difficulty on clearly identifying tumor boundaries in clinic. Conventional practice involves neurosurgeon’s visual evaluation and intraoperative histological examination of dissected tissues using frozen section, which is time-consuming and complex. The aim of this study was to develop fluorescent imaging coupled with artificial intelligence technique to quickly and accurately determine glioma in real-time during surgery. Methods Glioma patients (N = 23) were enrolled and injected with indocyanine green for fluorescence image–guided surgery. Tissue samples (N = 1874) were harvested from surgery of these patients, and the second near-infrared window (NIR-II, 1000–1700 nm) fluorescence images were obtained. Deep convolutional neural networks (CNNs) combined with NIR-II fluorescence imaging (named as FL-CNN) were explored to automatically provide pathological diagnosis of glioma in situ in real-time during patient surgery. The pathological examination results were used as the gold standard. Results The developed FL-CNN achieved the area under the curve (AUC) of 0.945. Comparing to neurosurgeons’ judgment, with the same level of specificity >80%, FL-CNN achieved a much higher sensitivity (93.8% versus 82.0%, P < 0.001) with zero time overhead. Further experiments demonstrated that FL-CNN corrected >70% of the errors made by neurosurgeons. FL-CNN was also able to rapidly predict grade and Ki-67 level (AUC 0.810 and 0.625) of tumor specimens intraoperatively. Conclusion Our study demonstrates that deep CNNs are better at capturing important information from fluorescence images than surgeons’ evaluation during patient surgery. FL-CNN is highly promising to provide pathological diagnosis intraoperatively and assist neurosurgeons to obtain maximum resection safely. Trial registration ChiCTR ChiCTR2000029402. Registered 29 January 2020, retrospectively registered

scholarly journals Mueller-matrix-based polarization imaging and quantitative assessment of optically anisotropic polycrystalline networks

PLoS ONE ◽  
2019 ◽  
Vol 14 (5) ◽  
pp. e0214494 ◽  
Author(s):  
Mariia Borovkova ◽  
Larysa Trifonyuk ◽  
Volodymyr Ushenko ◽  
Olexander Dubolazov ◽  
Oleg Vanchulyak ◽  
...  
Keyword(s):  
Quantitative Assessment ◽  
Mueller Matrix ◽  
Polarization Imaging

Non-osseous soft tissue metastasis in the foot from renal cell carcinoma

2020 ◽  
Vol 13 (10) ◽  
pp. e236051
Author(s):  
Nusrat Jahan ◽  
Shabnam Rehman
Keyword(s):  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Renal Cell ◽  
Soft Tissues ◽  
Imaging Techniques ◽  
Musculoskeletal Symptoms ◽  
Systematic Evaluation ◽  
Pathological Examination ◽  
Cell Renal Cell Carcinoma ◽  
History Of

Metastatic tumours of the distal extremities, also known as acrometastases, are rare. The majority of the acrometastases involve bones—involvement of the soft tissues of the feet and hands is extremely rare. We report a case of clear cell renal cell carcinoma metastasised to the soft tissues of the foot. The patient presented with pain and swelling in his right foot. Diagnosis of acrometastases frequently gets delayed due to the rarity of this condition and resultant low clinical suspicion. Possibility of metastatic disease should be entertained as an important differential diagnosis when patients with a history of cancer present with musculoskeletal symptoms. A systematic evaluation incorporating thorough clinical assessment, advanced imaging techniques like MRI and pathological examination is critical to establish the diagnosis.

scholarly journals Case report: A Re-operation Case of Asymptomatic Left Giant Pheochromocytoma and Literature Review

2020 ◽  
Author(s):  
Wei Dai ◽  
Xiaoguang Liu ◽  
Jialiang Dai ◽  
Minqiang Chen ◽  
Jian Mo ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Case Report ◽  
Perioperative Period ◽  
Radical Resection ◽  
Pathological Diagnosis ◽  
Maximum Diameter ◽  
Pathological Examination ◽  
Complication Rates ◽  
Ki 67 ◽  
Biochemical Detection

Abstract Introduction: Pheochromocytoma (PCC) is a rare tumor which derives from adrenal medulla, when maximum diameter of pheochromocytoma is greater than 10CM, it is divided into Giant pheochromocytoma(GPCC), which is extremely rare and usually asymptomatic.Clinically, a huge and asymptomatic adrenal pheochromocytoma was usually misdiagnosed as other types of tumors, which result in notable increase of complication rates and death rates.Case presentation:In this case report, we described a clinically asymptomatic GPCC patient. Diagnosis:According to computed tomography (CT) scan, nuclear magnetic resonance(MR) scan, the patient is initially diagnosed as liposarcoma.After laparotomy, biochemical detection of catecholamine (CA) intermediate metabolites methoxyepinephrine (MN) and methoxy norepinephrine (NMN) and the pathological examination , diagnosis of GPCC was obtained. Intervention：The laparotomy was suspended and a diagnosis of pheochromocytoma was confirmed, because of unstable blood pressure and hypertension during separating and moving the mass during surgery. After consultation of multi-disciplinary team, adequate preoperative preparation was conducted according to the procedure of PCC surgical preparation. By the end of the regular phenoxybenzamine and intravenous fluids treatment for three weeks, the blood pressure of the patient was kept at an acceptable average. Therefore, the patient underwent the operationagain for radical resection. Outcome: After the operation, CA was basically normal in rechecking process, and the tumor was successfully removed. Pathological diagnosis of the mass after operation: Immunohistochemical resulted conformed to (epigastrium) pheochromocytoma. Immunohistochemistry: CgA(+), Inhibin-α(-), ki-67(<1%+), Syn(+). Pheochromocytoma had the definite pathological diagnosis. Conclusion: GPCC has the high diagnostic identification difficulty, it should be combined with imaging examination and biochemical measurement to identify. Before operation, the detailed imageological examination provides important reference for excision and surgical planning. Individualized and multi-disciplinary cooperation of management strategy in perioperative period should be recommended. Patients should conduct long-term follow-up.

scholarly journals Mueller Matrix Polarimetric Imaging Analysis of Optical Components for the Generation of Cylindrical Vector Beams

Crystals ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 1155
Author(s):  
Guadalupe López-Morales ◽  
María del Mar Sánchez-López ◽  
Ángel Lizana ◽  
Ignacio Moreno ◽  
Juan Campos
Keyword(s):  
Liquid Crystal ◽  
Imaging Techniques ◽  
Mueller Matrix ◽  
Imaging Analysis ◽  
Optical Components ◽  
Spatial Homogeneity ◽  
Polarimetric Imaging ◽  
Vector Beams ◽  
Generate Vector

In this work, we performed a Mueller matrix imaging analysis of two commercial optical components usually employed to generate and manipulate vector beams—a radial polarizer and a liquid-crystal q-plate. These two elements generate vector beams by different polarization mechanisms—polarizance and retardance, respectively. The quality of the vector beams relies on the quality of the device that generates them. Therefore, it is of interest to apply the well-established polarimetric imaging techniques to evaluate these optical components by identifying their spatial homogeneity in diattenuation, polarizance, depolarization, and retardance, as well as the spatial variation of the angles of polarizance and retardance vectors. For this purpose, we applied a customized imaging Mueller matrix polarimeter based on liquid-crystal retarders and a polarization camera. Experimental results were compared to the numerical simulations, considering the theoretical Mueller matrix. This kind of polarimetric characterization could be very helpful to the manufacturers and users of these devices.

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