scholarly journals Development of a poly(arylene sulfide sulfone) antibacterial electrospun film as a skin wound dressing application

2021 ◽  
Author(s):  
Xuzhi Lang ◽  
Zhurong Tang ◽  
Zhimei Wei ◽  
Xiaojun Wang ◽  
Shengru Long ◽  
...  
Keyword(s):  
Mechanical Property ◽  
Zno Nanoparticles ◽  
Wound Dressing ◽  
Skin Wound ◽  
Dead Cell ◽  
Skin Wound Healing ◽  
High Mechanical Property ◽  
Water Contact ◽  
Transmission Electron ◽  
Dispersion Solution

Tissue engineering has become a hot issue for skin wound healing because it can be used as an alternative treatment to traditional grafts. Nanofibrous films have been widely used due to their excellent properties. In this work, an organic/inorganic composite poly(arylene sulfide sulfone)/ZnO/graphene oxide (PASS/ZnO/GO) nanofibrous film was fabricated with the ZnO nanoparticles blending in an electrospun solution and post-treated with the GO deposition. The optimal PASS/ZnO/GO nanofibrous film was prepared by 2% ZnO nanoparticles, 3.0[Formula: see text]g/mL PASS electrospun solution, and 1% GO dispersion solution. The morphology, hydrophilicity, mechanical property, and cytotoxicity of the PASS/ZnO/GO nanofibrous film were characterized by using scanning electron microscopy, transmission electron microscope, water contact angle, tensile testing, and a Live/Dead cell staining kit. It is founded that the PASS/ZnO/GO nanofibrous film has outstanding mechanical properties and no cytotoxicity. Furthermore, the PASS/ZnO/GO nanofibrous film exhibits excellent antibacterial activity to both Escherichia coli and Staphylococcus aureus. Above all, this high mechanical property in the non-toxic and antibacterial nanofibrous film will have excellent application prospects in skin wound dressing.

scholarly journals Keratin Scaffolds Containing Casomorphin Stimulate Macrophage Infiltration and Accelerate Full-Thickness Cutaneous Wound Healing in Diabetic Mice

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2554
Author(s):  
Marek Konop ◽  
Anna K. Laskowska ◽  
Mateusz Rybka ◽  
Ewa Kłodzińska ◽  
Dorota Sulejczak ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Dressing ◽  
Healing Process ◽  
Skin Wound ◽  
Wound Healing Process ◽  
Diabetic Mice ◽  
Full Thickness ◽  
Skin Wound Healing ◽  
Impaired Wound Healing

Impaired wound healing is a major medical challenge, especially in diabetics. Over the centuries, the main goal of tissue engineering and regenerative medicine has been to invent biomaterials that accelerate the wound healing process. In this context, keratin-derived biomaterial is a promising candidate due to its biocompatibility and biodegradability. In this study, we evaluated an insoluble fraction of keratin containing casomorphin as a wound dressing in a full-thickness surgical skin wound model in mice (n = 20) with iatrogenically induced diabetes. Casomorphin, an opioid peptide with analgesic properties, was incorporated into keratin and shown to be slowly released from the dressing. An in vitro study showed that keratin-casomorphin dressing is biocompatible, non-toxic, and supports cell growth. In vivo experiments demonstrated that keratin-casomorphin dressing significantly (p < 0.05) accelerates the whole process of skin wound healing to the its final stage. Wounds covered with keratin-casomorphin dressing underwent reepithelization faster, ending up with a thicker epidermis than control wounds, as confirmed by histopathological and immunohistochemical examinations. This investigated dressing stimulated macrophages infiltration, which favors tissue remodeling and regeneration, unlike in the control wounds in which neutrophils predominated. Additionally, in dressed wounds, the number of microhemorrhages was significantly decreased (p < 0.05) as compared with control wounds. The dressing was naturally incorporated into regenerating tissue during the wound healing process. Applied keratin dressing favored reconstruction of more regular skin structure and assured better cosmetic outcome in terms of scar formation and appearance. Our results have shown that insoluble keratin wound dressing containing casomorphin supports skin wound healing in diabetic mice.

scholarly journals A Novel Multilayer Composite Membrane for Wound Healing in Mice Skin Defect Model

Polymers ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 573 ◽  
Author(s):  
Yuyu Qiu ◽  
Qingqing Wang ◽  
Yajun Chen ◽  
Shufang Xia ◽  
Wei Huang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Dressing ◽  
Healing Process ◽  
Animal Experiments ◽  
Skin Wound ◽  
Control Group ◽  
Skin Defect ◽  
Multilayer Composite ◽  
Defect Model ◽  
Skin Wound Healing

To develop a wound dressing material that conforms to the healing process, we prepared a multilayer composite (MC) membrane consisting of an antibacterial layer (ABL), a reinforcement layer (RFL), and a healing promotion layer (HPL). Biocompatible zein/ethyl cellulose (zein/EC) electrospun nanofibrous membranes with in situ loaded antibacterial photosensitizer protoporphyrin (PPIX) and healing promotion material vaccarin (Vac) were, respectively, chosen as the ABL on the surface and the HPL on the bottom, between which nonwoven incorporated bacterial cellulose (BC/PETN) as the HPL was intercalated to enhance the mechanical property. Photodynamic antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa was confirmed by the enlarged inhibition zones; meanwhile, satisfactory biocompatibility of the HPL was verified by scanning electronic microscopy (SEM) of L929 cells cultured on its surface. The potential effects on wound healing in a mice skin defect model of the MC membranes were also evaluated. The animal experiments demonstrated that the wound healing rate in the MC group was significantly increased compared with that in the control group (p < 0.05). Histopathological observation revealed an alleviated inflammatory response, accompanied with vascular proliferation in the MC group. The MC membranes significantly promoted wound healing by creating an antibacterial environment and promoting angiogenesis. Taken together, this MC membrane may act as a promising wound dressing for skin wound healing.

scholarly journals Electrospun PCL-Surgihoney meshes for skin wound healing applications

2020 ◽  
Vol 318 ◽  
pp. 01029
Author(s):  
Enes Aslan
Keyword(s):  
Wound Dressing ◽  
Cell Attachment ◽  
Positive Impact ◽  
Dermal Fibroblast ◽  
Healing Process ◽  
Human Dermal Fibroblast ◽  
Skin Wound ◽  
Regulatory Function ◽  
Skin Wound Healing ◽  
Skin Healing

Skin is a complex and very important tissue, playing a significant protective and regulatory function. It is also prone to a large number of wounds and defects due to external factors such as temperature, chemical agents, and radiation. Accelerating the skin healing process and minimizing the risk of infection is a relevant research challenge. This paper investigates a novel wound dressing based on polycaprolactone (PCL), a synthetic biocompatible and biodegradable polymer, and honey-Surgihoney® (SH). Solution electrospinning was used to produce the wound dressing meshes. Different polymer solutions were prepared by mixing PCL and SH with acetic acid. Human dermal fibroblast were used to assess the biological characteristics of the electrospun meshes. Results show that the presence of SH1 has a positive impact on cell attachment and proliferation.

scholarly journals Wound Dressing Model of Human Umbilical Cord Mesenchymal Stem Cells-Alginates Complex Promotes Skin Wound Healing by Paracrine Signaling

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Song Wang ◽  
Huachao Yang ◽  
Zhenrui Tang ◽  
Gang Long ◽  
Wen Huang
Keyword(s):  
Wound Healing ◽  
Wound Dressing ◽  
Healing Rate ◽  
Skin Wound ◽  
Human Umbilical Cord ◽  
Paracrine Signaling ◽  
Skin Wound Healing ◽  
Alginate Gel ◽  
Calcium Alginate Gel ◽  
Wound Healing Rate

Purpose. To probe growth characteristics of human umbilical cord mesenchymal stem cells (hUCMSCs) cultured with alginate gel scaffolds, and to explore feasibility of wound dressing model of hUCMSCs-alginates compound.Methods. hUCMSCs were isolated, cultured, and identified in vitro. Then cells were cultivated in 100 mM calcium alginate gel, and the capacity of proliferation and migration and the expression of vascular endothelial growth factors (VEGF) were investigated regularly. Wound dressing model of hUCMSCs-alginate gel mix was transplanted into Balb/c mice skin defects. Wound healing rate and immunohistochemistry were examined.Results. hUCMSCs grew well but with little migration ability in the alginate gel. Compared with control group, a significantly larger cell number and more VEGF expression were shown in the gel group after culturing for 3–6 days (P< 0.05). In addition, a faster skin wound healing rate with more neovascularization was observed in the hUCMSCs-alginate gel group than in control groups at 15th day after surgery (P< 0.05).Conclusion. hUCMSCs can proliferate well and express massive VEGF in calcium alginate gel porous scaffolds. Wound dressing model of hUCMSCs-alginate gel mix can promote wound healing through paracrine signaling.

scholarly journals Keratin Biomaterials in Skin Wound Healing, an Old Player in Modern Medicine: A Mini Review

Pharmaceutics ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 2029
Author(s):  
Marek Konop ◽  
Mateusz Rybka ◽  
Adrian Drapała
Keyword(s):  
Wound Healing ◽  
Wound Dressing ◽  
Chronic Wounds ◽  
Biomedical Application ◽  
Medical Problem ◽  
Modern Medicine ◽  
Skin Wound ◽  
Wound Dressings ◽  
Skin Wound Healing ◽  
Impaired Wound Healing

Impaired wound healing is a major medical problem. To solve it, researchers around the world have turned their attention to the use of tissue-engineered products to aid in skin regeneration in case of acute and chronic wounds. One of the primary goals of tissue engineering and regenerative medicine is to develop a matrix or scaffold system that mimics the structure and function of native tissue. Keratin biomaterials derived from wool, hair, and bristle have been the subjects of active research in the context of tissue regeneration for over a decade. Keratin derivatives, which can be either soluble or insoluble, are utilized as wound dressings since keratins are dynamically up-regulated and needed in skin wound healing. Tissue biocompatibility, biodegradability, mechanical durability, and natural abundance are only a few of the keratin biomaterials’ properties, making them excellent wound dressing materials to treat acute and chronic wounds. Several experimental and pre-clinical studies described the beneficial effects of the keratin-based wound dressing in faster wound healing. This review focuses exclusively on the biomedical application of a different type of keratin biomaterials as a wound dressing in pre-clinical and clinical conditions.

scholarly journals Hydrogel Preparation Methods and Biomaterials for Wound Dressing

Life ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1016
Author(s):  
Jingjing Su ◽  
Jiankang Li ◽  
Jiaheng Liang ◽  
Kun Zhang ◽  
Jingan Li
Keyword(s):  
Wound Dressing ◽  
Wound Repair ◽  
Chronic Wounds ◽  
Healing Process ◽  
3D Structure ◽  
Skin Wound ◽  
Wound Dressings ◽  
Wound Healing Process ◽  
Skin Wound Healing ◽  
Preparation Methods

Wounds have become one of the causes of death worldwide. The metabolic disorder of the wound microenvironment can lead to a series of serious symptoms, especially chronic wounds that bring great pain to patients, and there is currently no effective and widely used wound dressing. Therefore, it is important to develop new multifunctional wound dressings. Hydrogel is an ideal dressing candidate because of its 3D structure, good permeability, excellent biocompatibility, and ability to provide a moist environment for wound repair, which overcomes the shortcomings of traditional dressings. This article first briefly introduces the skin wound healing process, then the preparation methods of hydrogel dressings and the characteristics of hydrogel wound dressings made of natural biomaterials and synthetic materials are introduced. Finally, the development prospects and challenges of hydrogel wound dressings are discussed.

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